evaluation of amplified ribosomal dna restriction analysis as a method for the identification of botryosphaeria species.the polymerase chain reaction was used to amplify a rdna fragment containing the internal transcribed spacers (its1-5.8s-its2) and the d1/d2 variable domains of the 28s rdna from 10 species of the genus botryosphaeria (fungi, ascomycota). restriction analysis of the amplicons with frequent-cutting endonucleases (amplified ribosomal dna restriction analysis) allowed the definition of 12 rdna haplotypes. each of the rdna haplotypes could be unambiguously assigned to a single botryosphaeria species ...200515837376
multiple gene genealogies and phenotypic data reveal cryptic species of the botryosphaeriaceae: a case study on the neofusicoccum parvum/n. ribis complex.neofusicoccum parvum and n. ribis (botryosphaeriaceae, ascomycetes) are closely related, plant pathogenic fungi with a world-wide distribution on a wide range of woody hosts. species boundaries in the n. parvum/n. ribis complex have eluded definition, despite the application of various tools for characterisation. in this study, we test the hypothesis that only one species exists amongst isolates from the n. parvum/n. ribis complex, identified from syzygiumcordatum trees across their native distr ...200919152837
molecular and phenotypic characterization of three phylogenetic species discovered within the neofisicoccum parvum/n. ribis complex.neofusicoccum parvum and n. ribis are closely related species whose identities often have been confused. these fungal plant pathogens were identified recently as the most abundant species of botryosphaeriaceae (ascomycetes) isolated from native syzygium cordatum trees in south africa. in another study using multiple gene genealogies from five nuclear loci three undescribed cryptic phylogenetic species as well as n. parvum were identified among 30 of these isolates. the aim of this study was to c ...200919750943
a six locus phylogeny reveals high species diversity in botryosphaeriaceae from california almond.botryosphaeriaceae are important pathogens on a variety of woody hosts, including almond, a major crop in california. almond is susceptible to botryosphaeria dothidea that forms band cankers on almond trunks, and the same fungus was also isolated from cankers of the canopy. to study the diversity and host range of b. dothidea and allied species from almond we used 132 isolates from 36 plant hosts from five continents, including 45 strains from almond in california. species were identified by com ...201020943551
use of the genealogical sorting index (gsi) to delineate species boundaries in the neofusicoccum parvum-neofusicoccum ribis species complex.neofusicoccum is a recently described genus of common endophytes and pathogens of woody hosts, previously placed in the genus botryosphaeria. many morphological characteristics routinely used to describe species overlap in neofusicoccum, and prior to the use of molecular phylogenetics, isolates from different hosts and locations were often misidentified. two cryptic species neofusicoccum ribis and neofusicoccum parvum were initially described from different continents and recently another four s ...201121575732
genetic and pathogenic diversity of neofusicoccum parvum in new zealand vineyards.genetic diversity of 50 isolates of neofusicoccum parvum, the predominant species of the botryosphaeriaceae recovered from grapevines displaying symptoms of dieback and decline in new zealand, was compared to that of isolates from australia, south africa, and california. the eight universally primed polymerase chain reaction (up-pcr) primers distinguished 56 genotypes, with only four clonal pairs found. seven main groups were identified in a neighbour-joining (nj) tree with isolates from differe ...201122289773
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