| [cross-sectional anatomy of miniature "shiba" goat]. | the anatomical relations of visceral organs and other tissues in the thoracic, abdominal and pelvic cavities of the male miniature "shiba" goat are illustrated as thirteen semi-diagramatic representations of cross sections. | 1979 | 428446 |
| genetic variants of glucose phosphate isomerase (e.c. 5.3.1.9) in canine erythrocytes. | genetic polymorphism of glucose phosphate isomerase (gpi) was found in the erythrocytes of dogs of six japanese breeds by using starch gel electrophoresis. analysis of parentage records of dogs revealed that the phenotypic variation of erythrocyte glucose phosphate isomerase was controlled by one autosomal locus with two codominant alleles, gpia and gpib. the allele gpib was observed in the following breeds: san'in-shiba, shinshu-shiba, shikoku, kai and kishu, but not in hokkaidoes and akitas. a ... | 1977 | 616784 |
| difference in form of sialic acid in red blood cell glycolipids of different breeds of dogs. | hematoside from dog erythrocyte membrane was previously considered to contain a mixture of n-acetyl- and n-glycolyl- neuraminic acids. however, the hematoside preparation used in the previous study was obtained from pooled blood of several dogs, and individual variation in hematoside was not examined. in this work, hematosides of erythrocytes from 31 mongrel dogs and 108 dogs of 23 breeds were examined individually by thin-layer chromatography, and the component sialic acids were analysed by gas ... | 1978 | 659384 |
| abnormal structure of the canine oncogene, related to the human c-yes-1 oncogene, in canine mammary tumor tissue. | cellular oncogenes of genomic dna in 6 canine primary mammary tumors were screened by southern blot analysis, using 7 oncogene probes. a canine genomic oncogene related to the human c-yes-1 oncogene was detected as abnormal bands in solid carcinoma genomic dna digested with ecori, hindiii, hindiii-ecori, or hindiii-bamhi. comparison was made between other tumor specimens and control specimens obtained from 4 clinically normal dogs--1 mixed breed and 3 shiba inu dogs (the same breed as the dog fr ... | 1991 | 1789521 |
| [effect of maternal oxygenation on fetal blood gas status in shiba goat fetuses]. | the purpose of this study is to investigate the effect of maternal oxygen administration on the fetal blood gas status. five shiba goat fetuses were used whose gestational days were 134 +/- 4 days. maternal and fetal ph, po2, pco2, b.e., and hematocrit were measured every 30 minutes during 4 l/min oxygen administration for 60 minutes. maternal arterial blood po2 increased from 95.5 +/- 19.6 mmhg to 262.6 +/- 75.8 mmhg at 30 minutes and to 207.8 +/- 110.1 mmhg at 60 minutes. fetal arterial blood ... | 1990 | 2126025 |
| stereotaxic approach to hypothalamic nuclei of the shiba goat with radiographic monitoring. | practical method was devised for precise approach to hypothalamic nuclei in the shiba goat. a stereotaxic instrument and a brain atlas with stereotaxic coordinates were developed. for an accurate placement of probes into specific hypothalamic regions a radiographic method was employed in which radio-opaque material was injected into the lateral ventricle and the ventricular outline was depicted. a sagittal diagram showing the arrangement of hypothalamic nuclei in relation to the brain ventricula ... | 1990 | 2190033 |
| a positive regulatory sequence of the saccharomyces cerevisiae eno1 gene. | eno1-'lacz fusions with various lengths of the eno1 5'-flanking region were constructed on various types of yeast plasmid vectors. the fully expressed level of beta gal directed by eno1-'lacz fusions differed depending on the type of vector, but on any type of vector, beta gal activity was not greatly influenced by the carbon source in the medium. the 86-bp dna region of eno1 at position -487 to -402 upstream of the initiation codon, in which we had previously delimited the positive regulatory r ... | 1987 | 3117780 |
| outbreak of pneumonia caused by pasteurella haemolytica infection in shiba goats in japan. | | 1988 | 3172608 |
| photoperiodic modification of negative and positive feedback effects of oestradiol on lh secretion in ovariectomized goats. | ovariectomized shiba goats carrying an oestradiol implant (4-10 pg/ml) were kept under a short-day light regimen (10l:14d; group 1, n = 4) or a long-day regimen (16l:8d; group 2, n = 4). plasma lh concentrations were lower (p less than 0.05) in group 2 than in group 1 between days 40 and 200, suggesting an enhanced negative feedback effect of oestradiol on lh secretion under a long-day regimen. on days 30, 60, 100, 149 and 279, an lh surge was induced by i.v. infusion of oestradiol for 48 h; the ... | 1987 | 3656284 |
| [profile of a nurse. ms, kazumi shiba who assisted in ophthalmic surgery in a village in nepal]. | | 1981 | 6172614 |
| changes in plasma concentrations of lh, progesterone and oestradiol in relation to the occurrence of luteolysis, oestrus and time of ovulation in the shiba goat (capra hircus). | luteolysis in shiba goats was spontaneous (n = 5) or induced by prostaglandin f-2 alpha (n = 5). blood sampling and the test for oestrous behaviour were carried out at 2-h intervals, and the time of follicular rupture (ovulation) was determined by culdoscopic observations performed every 2 h around the periovulatory period. in both groups plasma lh concentrations showed a temporary but significant increase during the abrupt fall in plasma progesterone concentrations at luteolysis. this lh rise m ... | 1984 | 6540807 |
| [gene constitution of miniature "shiba" goats (author's transl)]. | genetic surveys were carried out on the miniature "shiba" goats which have been raised in nagasaki prefecture, japan. recently, in thhe prefecture the number of "shiba" goats have been markedly decreased to totally about 190 which are kept now only in two towns. morphological genetic traits were observed to have been coming homogenous in nagasaki prefecture, and homogeneity of the traits in the colonies kept the national institute of animal industry and in stock farm of university of tokyo, espe ... | 1978 | 720392 |
| genetic polymorphism of eserine resistant esterases in canine plasma. | six plasma eserine resistant esterase phenotypes were observed in a population of 1438 dogs consisting of 38 breeds. analysis of parentage records of the dogs examined revealed that the phenotypic variation of eserine resistant esterases was controlled by 3 codominant alleles esa, esb and esc at one autosomal locus. the gene frequency of esb was high in most of the breeds examined. allele esc was only seen in 5 japanese native breeds, akita, shikoku, hokkaido, shinshu-shiba and mino-shiba, and i ... | 1977 | 603095 |
| placental type of membrane-bound arylamidase (shiba isoenzyme) found in human cancerous lung. | a placental type of membrane-bound arylamidase (shiba isoenzyme) was found in the tissue of adenocarcinoma of the lung. cancerous lung membrane-bound arylamidase had the same electrophoretic mobility, isoelectric point (ph 4.2), and susceptibility to sds inactivation as placental membrane-bound arylamidase. cancerous lung membrane-bound arylamidase had molecular weight and kinetic properties similar to normal lung membrane-bound arylamidase, but differed from the normal lung membrane-bound aryla ... | 1979 | 436290 |
| effects of timed melatonin infusion on prolactin secretion in pineal denervated goat. | the effects of timed melatonin infusion on prolactin secretion were examined in the pineal denervated goat. ovariectomized shiba goats (n = 5) were subjected to bilateral superior cervical ganglionectomy (scgx); this procedure resulted in complete abolition of endogenous melatonin release during the dark phase. scgx goats failed to coordinate their prolactin secretion with the prevailing photoperiod. melatonin was infused (20 micrograms/h, s.c.) daily into these goats either for 8 h (the long-da ... | 1986 | 3958896 |
| nonsurgical collection of embryos in shiba goats. | a method of nonsurgical embryo collection in the shiba goat, a native japanese miniature goat breeding nonseasonally, was developed. the apparatus used for flushing the uterus was made on the model of the two-way catheter for cows. embryo collection was performed on days 5 to 7 in 37 females superovulated with pmsg and hcg and resulted in successful recovery of 69 embryos in 19 females (51.4%). the average number of embryos collected from each successful female was 3.6. the recovery rate of embr ... | 1987 | 3816989 |
| three-dimensional structure of the sertoli cell in the shiba goat. | the three-dimensional structure of the sertoli cell in the miniature "shiba" goat was examined using scanning electron microscopy. in the basal portion of the seminiferous epithelium, spermatogonia and/or spermatocytes were located in compartments enclosed by adjacent sertoli cells. from the basal aspect, they were situated in successive recesses. in the middle portion, early round spermatids halfway embedded in the sertoli cell were recognized. the exposed surfaces of these spermatids were wrap ... | 1987 | 3439849 |
| involvement of melatonin in the seasonal changes of the gonadal function and prolactin secretion in female goats. | ovarian cyclicity in the goat continues under short days (8l: 16d), while it ceases for 150-200 days under long days (16l: 8d). during the anovulatory period under long days, prolactin secretion is enhanced. experiments were conducted to investigate the role of the pineal gland in the photoperiodic control of the gonadal function and prolactin secretion in the goat. firstly, the effects of the abolition of the diurnal change in melatonin secretion on the photoperiodic responses of the gonadal ax ... | 1988 | 3413343 |
| production and immunohistochemical characterization of specific antibody to oviductal fluid of miniature 'shiba' goat. | monoclonal antibodies were produced against the oviductal secretory substances in 'shiba' goats and their nature was characterized. in the ampullary region, very strong immunoreactivity with one clone (ee-1) was localized within the cytoplasm of non-ciliated cells ("secretory cells"), whereas very little or no immunoreactivity was observed in the isthmus region. this reactivity pattern was observed in both estrous and luteal phases. immunohistochemical analysis showed the antigen to be either ab ... | 1995 | 7548393 |
| chemical structure and biologic activity of bacterial and synthetic lipid a. | the chemical structure of the lipid a component of enterobacterial lipopolysaccharide (lps) is now known in some detail. for example, lipid a of escherichia coli consists of a beta(1----6)-linked d-glucosamine disaccharide that carries four (r)-3-hydroxytetradecanoyl groups in positions 2, 3, 2', and 3' and two phosphoryl residues in positions 1 and 4'. the hydroxy fatty acids at positions 2' and 3' are acylated at their 3-hydroxyl groups by dodecanoic acid and tetradecanoic acid, respectively. ... | 1987 | 3317748 |
| immune response of rabbits to lipid a: influence of immunogen preparation and distribution of various lipid a specificities. | sixty-two rabbit anti-lipid a serum samples were compared with respect to the immunogens used (synthetic lipid a and partial structures, natural lipid a, or acid-treated bacteria). immunoglobulin (ig) type-specific differences in rabbit response between liposomal membrane-embedded (lme) and other lipid a immunogens were found: lme lipid a elicited predominantly igm antibodies. previous findings of equally good immune responses to synthetic lipid a and acid-treated bacteria (l. brade, e.t. rietsc ... | 1993 | 7678589 |
| genetic control of glycolipid expression. | a polymorphic variation of sialic acid species of sialosyllactosylceramide was found in dog erythrocytes. the analysis of the glycolipids in the erythrocytes of the individual dogs in a family of a japanese breed of dog, shiba-inu, showed that the expression of sialosyllactosylceramide containing n-glycolylneuraminic acid was an autosomal dominant trait over the expression of that containing n-acetylneuraminic acid. polymorphic variations of major liver gangliosides were also found in various st ... | 1986 | 3103940 |
| the secy protein can act post-translationally to promote bacterial protein export. | conditionally lethal escherichia coli mutants in secy (prla) show defective export of proteins to the periplasm and outer membrane. it has been proposed that this gene and other sec genes must act on pro-ompa at an early stage of protein synthesis in order to allow later translocation to occur. we have described a temperature-sensitive mutation in which the secyts function is impaired at the nonpermissive temperature (ito, k. (1984) mol. gen. genet. 197, 204-208). a plasmid bearing the wild-type ... | 1986 | 3017993 |
| the evidence of carotid body in the carotid rete of the shiba goat. | the carotid body was found in/on the cavernous sinus of the carotid rete of the japanese miniature shiba goat by light and electron microscopies. the carotid body-like aggregated cell group consisted of two types of cells arranged in the lobule. the first type contained membrane-bound granular vesicles; these cells resembled to the type-i cells in the carotid body. the second type contained no cytoplasmic vesicles, and were located in the periphery, investing the type-i cells. these features app ... | 1994 | 7978348 |
| breed differences in the phenotype and gene frequencies in canine d blood group system. | the d system of canine blood groups was studied in 3,191 dogs of many different breeds. the frequencies of the d system phenotypes and genes were measured. these frequencies varied considerably between the breeds native to japan. the frequency of the d1 phenotype was higher in breeds native to japan than in those of non-japanese origin. conversely, non-japanese breeds generally had the d2 phenotype. the dogs described as mongrel in japan had d system frequencies intermediate between native japan ... | 1994 | 7999880 |
| suppressors of the secy24 mutation: identification and characterization of additional ssy genes in escherichia coli. | we previously reported (shiba et al., j. bacteriol. 160:696-701, 1984) the isolation and characterization of the mutation (ssy) that suppresses the protein export defect due to the secy24(ts) mutation and causes cold-sensitive growth of escherichia coli. this report describes more systematic isolation of ssy mutations. among temperature-resistant revertants of the secy24 mutant, 65 mutants were found to be cold sensitive. these cold-sensitive mutations have been classified by genetic mapping. tw ... | 1986 | 3011749 |
| three-dimensional structure of some sertoli cell organelles in the cotton rat and shiba goat. | the three-dimensional structure of some sertoli cell organelles in the cotton rat and shiba goat was observed under a scanning electron microscope using the osmium-dimethyl sulfoxide-osmium method. mitochondria of the sertoli trunk in the scanning electron microscopic (sem) observation appeared much longer and more densely distributed than those in the transmission electron microscopic (tem) observation and had a different shape from oval mitochondria in the basal region of the sertoli cell. the ... | 1989 | 2680060 |
| structural requirements of lipid a for endotoxicity and other biological activities. | for the past ten years, several groups were engaged in synthetic studies of lipid a, namely the lipid portion of bacterial lipopolysaccharides (lps) that has been assumed to be the bioactive center of lps, but has not been unanimously approved. among them, shiba, kusumoto, and colleagues, osaka, japan have synthesized most energetically and successfully a variety of counterparts of lipid as, biosynthetic lipid a precursors, and their analogs. the endotoxic and related bioactivities of these synt ... | 1989 | 2663021 |
| [diurnal rhythms of body temperature, heart rate, and behaviour in the stanchioned adult shiba goats]. | using a data logger system, body temperature (dorsal subcutaneous temperature), heart rate, ingestive or ruminating behaviour and posture in adult shiba goats tied to a stanchion were recorded automatically during 24 hours, to obtain basic information on the biological rhythms. a 600 g of usual ration mixed with hay cube, hay, beet pulp and wheat bran was fed twice a day (morning; 9:00-9:30, evening; 16:00-16:30). animals kept under an artificial photoperiod (12l-12d, light period; 5:30-17:30) a ... | 1990 | 2361524 |
| lectin-binding patterns in the spermatogenic cells of the shiba goat testis. | lectin-binding patterns in the testis of the sexually mature goat were investigated by light and transmission electron microscopy. dolichos biflorus agglutinin (dba) and griffonia simplicifolia agglutinin-i (gs-i) were negative in the seminiferous epithelium, but soybean (glycine max) agglutinin (sba), griffonia simplicifolia agglutinin-ii (gs-ii) and peanut (arachis hypogaea) agglutinin (pna) were positive in the acrosomal vesicle of the golgi-phase spermatids and in the acrosome of the cap-, a ... | 1991 | 1836378 |
| molecular cloning of cdna encoding the c-kit receptor of shiba goats and a novel alanine insertion specific to goats and sheep in the kinase insert region. | the complete open reading frame (orf) of the c-kit cdna was cloned from a cerebellar cdna library of the shiba goat (capra hircus var shiba) with the dominant black-eyed white phenotype. the analysis of the deduced amino acid sequence revealed the presence of a single amino acid insertion (alanine) in the kinase insert (ki) region. while the newly found alanine insertion is not correlated with the coat color phenotype of goats, it appears to be characteristic of the c-kit genes in goats and shee ... | 1997 | 9199245 |
| hereditary high-potassium erythrocytes with high na, k-atpase activity in japanese shiba dogs. | the sodium (na) and potassium (k) concentrations and na, k-atpase activity were examined in erythrocytes from 24 japanese shiba dogs and 79 dogs of 24 other breeds. eleven of the shibas had erythrocytes with high k and low na concentrations, together with high na, k-atpase activity (hk rbcs), while red cells from the remaining shibas and all of the other breeds examined showed low k and high na concentrations, with no enzyme activity (lk rbcs). the concentration of reduced glutathione in hk rbcs ... | 1991 | 1646472 |
| molecular cloning of caprine il-6 cdna and its expression in insect cells. | to analyze the interactions among immunoneuroendocrinological systems in a miniature shiba goat, we attempted to clone and express caprine cdna-encoding interleukin-6 (il-6) which is well-known to exert a variety of effects on neuroendocrinological functions in small experimental animals. the cdna-encoding caprine il-6 was molecularly cloned from a lipopolysaccharide-stimulated adherent splenocyte library by the reverse transcription-polymerase chain reaction. the sequence analysis shows that th ... | 1997 | 9250586 |
| siblings with normal ldl receptor activity and severe hypercholesterolemia. | we report about a brother and sister having clinical symptoms similar to those of homozygous familial hypercholesterolemia (fh) but surprisingly who have normal low density lipoprotein (ldl) receptor activities (m. harada-shiba et al, j jpn atheroscler soc 1991; 19:227-242). the ldl receptor activities in the cultured fibroblasts of the patients were compared with those of fh heterozygotes and homozygotes for the ldl receptor mutation. the ldl receptor activities in the cultured fibroblasts of t ... | 1992 | 1525122 |
| genetic variation and interspecific hybridization among natural populations of zoysiagrasses detected by rflp analyses of chloroplast and nuclear dna. | genetic variations among 17 accessions of zoysiagrasses collected from natural populations in japan were investigated by rflp analyses of chloroplast dna (cpdna) and nuclear dna. these accessions were classified into five species based on morphological characteristics: zoysia japonica, z. matrella, z. tenuifolia, z. sinica, and z. macrostachya. on the basis of eight kinds of rflps in cpdnas detected across accessions, six chloroplast genome types (types a-f) were identified. although type-a cpdn ... | 1997 | 9418258 |
| influence of dietary protein concentrations or of duodenal amino acid infusion on cholecystokinin release in goats. | 1. whether dietary protein levels or duodenal infusion of amino acids alters the release of cholecystokinin (cck) in blood plasma of goats was investigated in three experiments. the cck determination was done by radioimmunoassay with specific cck-8 antibody. the male adult shiba goat, a miniature japanese native goat, was used. 2. in experiment 1, four goats were offered a diet containing 4.94 g crude protein (cp)/kg bw0.75 for the first 7 days. they were then given a diet containing 5.86 g cp/k ... | 1992 | 1348686 |
| [biological characteristics of miniature "shiba" goats (author's transl)]. | practical informations of miniature "shiba" goats, that have since long been bred for meat consumption in kyushu district of japan, were provided for using as laboratory animals. nine years ago, some pairs of the goats, were introduced to stock farm of tokyo university from a national institute. thereafter, they were successfully bred and increased in number and were distributed to many laboratories for researchers. the "shiba" goat is white coloured, horned, and much smaller in size while shari ... | 1977 | 908359 |
| gm1 gangliosidosis in shiba dogs. | a six-month-old shiba dog with a one-month history of progressive motor dysfunction showed clinical signs of a cerebellar disorder, including ataxia, dysmetria and intention tremor of the head. histopathological and ultrastructural studies revealed distended neurons packed with membranous cytoplasmic bodies throughout the central nervous system. the activities of lysosomal acid beta-galactosidase in its leucocytes and liver were less than 2 per cent of the control levels, and the compound accumu ... | 2000 | 10887996 |
| involvement of inhibin in the regulation of follicle-stimulating hormone secretion in the young adult male shiba goat. | the roles of inhibin and testosterone in the regulation of gonadotropin secretion were investigated in young adult male shiba goats (8-12 months of age). plasma levels of inhibin but not testosterone abruptly decreased after hemicastration (75% of the initial level), concomitant with a progressive rise in plasma levels of fsh. removal of the remaining testis at 33 days after the hemicastration quickly decreased plasma levels of both inhibin and testosterone and induced a progressive increase in ... | 2000 | 10901442 |
| a placental type of membrane-bound arylamidase in serum and ascites from a patient with renal cell carcinoma. | a placental type of membrane-bound arylamidase was detected in serum and ascites from a patient with renal cell carcinoma by isoelectric focusing. the enzyme had the same molecular weight, electrophoretic mobility on polyacrylamide gel, isoelectric point and km value as placental membrane-bound arylamidase. the new type of membrane-bound arylamidase was named shiba-isoenzyme after the patient's name. | 1978 | 699325 |
| [the fhr variability of "shiba goats" in acute hypoxic state]. | fetal heart rate variability in an experimentally induced acute hypoxic state was investigated using chronically prepared fetal goats. fhr variability was calculated using a microcomputer with the r-r intervals of the direct fetal ecg signal measured with a peak detector. the accuracy of the peak detector was 0.1 msec. the formula for fhr variability was as follows: (formula: see text) a fetal acute hypoxic state was induced by maternal inhalation of a gas mixture low in o2 concentration. the fe ... | 1984 | 6715936 |
| involvement of inorganic polyphosphate in expression of sos genes. | inorganic polyphosphate (poly(p)) is a linear polymer that has been found in every organism so far examined. to elucidate the functions of poly(p) in the regulation of gene expression, the level of cellular poly(p) in escherichia coli was reduced to a barely detectable concentration by overproduction of exopolyphosphatase (exopoly(p)ase) with a plasmid encoding yeast exopoly(p)ase (shiba et al., proc. natl. acad. sci. usa 94 (1997) 11210-11215). it was found that exopoly(p)ase-overproducing cell ... | 2000 | 10978509 |
| [effects of autonomic blockades, beta-adrenergic agent and cholinergic agent on the fhr variability of "shiba goats"]. | the effects of atropine, propranolol, isoproterenol and acetylcholine on the fetal heart rate variability were studied using chronically prepared fetal goats. when atropine, propranolol and atropine-propranolol were injected the stvs decreased by 45.2%, 43.2% and 57.3%, respectively; ltvs also decreased by 72.3%, 52.2% and 73.3%, respectively. stvs and ltvs increased by 39.5% and 34.5% with the injection of isoproterenol, and decreased by 27.6% and 22.7% with acetylcholine. these results suggest ... | 1984 | 6501966 |
| pituitary chromophobe carcinoma with a low level of serum gonadotropin and an aspermatogenesis in a dog. | a 5-year-old male shiba dog with progressive neurologic signs was examined by computed tomography (ct). a ct image of the brain disclosed a large, spherical high-density lesion in the thalamus and diencephalon. serum lh, fsh and testosterone levels were all low. macroscopically the large mass was connected with the sella turcia, and it was histopathologically diagnosed as a pituitary chromophobe carcinoma. an aspermatogenesis was observed in the testes. therefore, it was suggested that the low l ... | 2001 | 11258457 |
| ghrelin in domestic animals: distribution in stomach and its possible role. | ghrelin, a novel growth-hormone-releasing acylated peptide, was recently isolated from rat and human stomachs. in rat, peripheral or central administration of ghrelin stimulates the secretion of growth hormone (gh) from the pituitary gland. recent work suggests that ghrelin plays an important role in energy homeostasis, body weight, and food intake. we examined the distribution of cells immunoreactive to ghrelin in the stomachs of domestic animals and rats, using a polyclonal antibody for the n- ... | 2001 | 11524171 |
| [on the trip to nagasaki by kokan shiba] (jpn). | | 1984 | 11621035 |
| mechanism of action of arenesulfonylhydrazones of 2-pyridinecarboxaldehyde 1-oxide in l1210 cells. | the arenesulfonylhydrazones of 2-pyridinecarboxaldehyde 1-oxide represent a relatively new class of anticancer agents. the biochemical alterations responsible for antineoplastic activity were investigated using the most potent member of this class synthesized to date, the 3,4-dimethoxybenzenesulfonylhydrazone of 2-pyridinecarboxaldehyde 1-oxide (3,4-dsp), as the prototype compound. the primary biochemical lesion observed was the production of dna single-strand breaks, which were analyzed using a ... | 1984 | 6498834 |
| taxonomy of phototrophic green and purple bacteria: a review. | the presently existing classification for the green and purple bacteria comprises physiological-ecological assemblages of phototrophic bacteria with anoxygenic photosynthesis. the taxonomic units of the different levels were based entirely on common phenotypic traits, including morphological, cytological, physiological and biochemical characteristics. since degrees of resemblance form the basis of the grouping, this classification cannot reflect the genetic or evolutionary relatedness of these b ... | 1983 | 6195952 |
| characterization of gh pulsatility in male shiba goats: effects of postpubertal castration and kp102. | the present study was conducted in order to characterize the secretory pattern of gh in the shiba goat, a native japanese miniature goat, and to examine the effects of castration and kp102, a gh secretagogue, on this pattern. blood samples were taken from an indwelling jugular catheter every 15 min for 24 h, and plasma gh was measured by radioimmunoassay. in intact males, gh was secreted in a pulsatile manner with very regular 5-h periodicity, which consisted of a distinctive gh pulse and a trou ... | 2002 | 12081232 |
| differences of phenotype and gene frequency by c system in canine species. | the canine c blood group was determined by an agglutination reaction with a plant agglutinin extracted from the seeds of clerodendron trichotomum (ctl). a positive agglutination reaction was classified as type c and a negative reaction as type c. in this study, the c phenotype and the gene frequency of c were examined in 377 dogs (224 purebred dogs of 29 breeds and 153 mongrel dogs) that were bred in utsunomiya-city, tochigi prefecture. phenotype c was less frequent than phenotype c in every pur ... | 1995 | 7492670 |
| cloning and primary structure of a new hemolysin gene from bacillus cereus. | a new hemolysin gene from bacillus cereus vkm-b164 was cloned in escherichia coli and sequenced. deduced protein consists of 219 amino acids and has a molecular mass of 24.4 kda. it has been concluded that the hemolysin, named 'hemolysin iii', is distinct from the b. cereus hemolysins reported previously: cereolysin, sphingomyelinase, cereolysin ab, hemolysin ii, and 'cereolysin-like' hemolysin (honda, t., shiba, a., seo, s., yamamoto, j., matsuyama, j. and miwatani, t. (1991) fems microbiol. le ... | 1995 | 7495855 |
| [discriminant analysis of the sexual differences in the skeletons in shiba dogs (canis familiaris)]. | | 1987 | 3618103 |
| influence of the processing of muca protein on the reversion of the frameshift hisd3052 and the base substitution hisg46 mutations by chemical mutagens in salmonella typhimurium. | the correlation between the proficiency at promoting mutagenesis of muca/b proteins and muca processing has been considered to be very high (hauser et al., 1992) on the basis of the results of uv mutagenicity (shiba et al., 1990). here we show that this correlation is only partial. we have assayed the mutagenicity of benzo[a]pyrene (b[a]p) and aflatoxin b1 (afb1) in salmonella typhimurium tester strains containing plasmids which encode muca proteins with an altered cleavage site. reversion of th ... | 1994 | 7508568 |
| [a case of tsutsugamushi disease which occurred in south western shikoku]. | we report a case of tsutsugamushi disease found in south western shikoku. a 64-year-old male who lived in towa village in kochi, developed a fever and headache on april 6, 1994, and was admitted to uwajima city hospital on april 15, with a ten-day history of illness. he had an eschar on the right anterior side of the breast and an enlargement of the right axillary lymph node, without a rash. laboratory data showed mild liver injury and atypical lymphocytes with 6% in peripheral blood. after his ... | 1995 | 7561256 |
| in vivo glycation of aldehyde reductase, a major 3-deoxyglucosone reducing enzyme: identification of glycation sites. | we have reported that the enzyme which reduces 3-deoxyglucosone (3-dg), a major intermediate and a potent cross-linker in the maillard reaction, is identical with aldehyde reductase [takahashi, m., fujii, j., teshima, t., suzuki, k., shiba, t., & taniguchi, n. (1993) gene 127, 249-253]. the enzyme purified from normal rat liver was found to be partially glycated as judged by binding to a boronate column and reactivity to anti-epsilon-hexitol lysine igg. sites of in vivo glycation of rat liver al ... | 1995 | 7827091 |
| melanocytes fail to survive in hair bulbs of the shiba goat (capra hircus) with the dominant black-eyed white phenotype. | dominant black-eyed white phenotypes are one of the most commonly observed traits in domestic animals. their genetic control mechanisms, however, have not been elucidated. as the first step to approach the problem, we examined histologically the patterns of the distribution of pigment cells in shiba goats (two each of day-73-postcoitum and day-112-postcoitum fetuses, and a 15-week-old kid) with the dominant black-eyed white phenotype. melanocytes were present and fully pigmented in the choroid a ... | 1994 | 7971748 |
| a novel mutation in the gene for canine acid beta-galactosidase that causes gm1-gangliosidosis in shiba dogs. | a homozygous recessive mutation, causing gm1-gangliosidosis in shiba dogs, was identified as a deletion of c nucleotide 1668 in the gene for canine acid beta-galactosidase, which was a novel mutation in canine gm1-gangliosidosis. | 2002 | 12555949 |
| inositol monophosphatase activity from the escherichia coli suhb gene product. | the suhb gene is located at 55 min on the escherichia coli chromosome and encodes a protein of 268 amino acids. mutant alleles of suhb have been isolated as extragenic suppressors for the protein secretion mutation (secy24), the heat shock response mutation (rpoh15), and the dna synthesis mutation (dnab121) (k. shiba, k. ito, and t. yura, j. bacteriol. 160:696-701, 1984; r. yano, h. nagai, k. shiba, and t. yura, j. bacteriol. 172:2124-2130, 1990; s. chang, d. ng, l. baird, and c. georgopoulos, j ... | 1995 | 8002619 |
| an ultrastructural study of the sertoli cell in the shiba goat. | the ultrastructure of the shiba goat sertoli cell was observed using light and transmission electron microscopy. in the basal portion of the sertoli cell, the nucleus was located near the basement membrane and was irregular in outline. a number of filaments with intermediate width were observed around the nucleus. a multivesicular nuclear body, composed of numerous vesicles of different sizes and ribosome-like structures, was detected within the nucleus. the majority of cell organelles was situa ... | 1994 | 8204759 |
| ovarian and hormonal response of female goats to active immunization against inhibin. | this study was conducted to evaluate the effect of active immunization against inhibin on hormonal levels and the ovulation rate in goats. ten adult shiba goats (capra hircus) in two groups were used in this study. the first group was injected with inhibin vaccine (immunized, n=5) and the second group was injected with freund's adjuvant (control, n=5) followed by three booster injections at 4-week intervals. after the third booster injection, three consecutive periods of oestrus were induced usi ... | 2003 | 12740017 |
| [effects of land utilization of sewage sludge on grass and soils]. | effects of land disposal of sewage sludge on grass and soil environment were studied. the sewage sludge used was from northern shenyang wastewater treatment plant. the results showed that contents of nutrient in the soil were increased after sewage sludge application, especially for organic matter. grass biomass were increased and the green period were extended with a better growth of the lawn. the heavy metal contents in the soil were increased with cd contents beyond 2nd grade national environ ... | 2003 | 12800658 |
| bilateral renal lymphosarcoma in a dog. | a lymphosarcoma originating from kidneys, which metastasized to the ovaries, oviducts, heart and pancreas, was pathologically diagnosed in a 3-year-old female japanese dog (shiba dog). clinically, the dog exhibited vomiting, diarrhea, oliguria and abdominal enlargement, associated with hypoglycemia. grossly, the kidneys were enlarged and neoplastic masses were also seen in the ovaries and heart. histologically, a majority of glomeruli and renal tubules were obliterated due to diffuse invasion by ... | 1993 | 8399750 |
| monitoring reproductive status by fecal progesterone analysis in ruminants. | characterization of the estrous cycle and the ability to detect pregnancy are essential for the development of efficient captive breeding programs in wild mammalian species and for their conservation. we assess here the feasibility of using fecal progesterone analysis as a non-invasive method of monitoring the ovarian function of the shiba goat as an experimental model for ruminant species. feces and blood samples were collected sequentially from 4 female goats throughout the estrous cycle and g ... | 1995 | 8593290 |
| development of rotation of mandibular premolar tooth germs in the dog. | the development of premolar tooth germs in dogs, particularly their rotation and crowding, was examined on radiographs, dissected specimens of tooth germs, serial sections stained by masson-goldner's technique and reconstructed models. the materials used were the mandibles of 32 puppies from birth to 6 months, and the species was a highly pedigreed shiba inu which has no crowding of the premolars in the mature stage. the results obtained are as follows. (1) rotations and crowdings of premolars, ... | 1982 | 7158282 |
| [the clinical values fro chemical constituents of blood in normal miniature shiba goats (author's transl)]. | using the both methods. raba-super system (rapid blood analyzer system), the clinical values for chemical constituents of blood in shiba goats bred in stock farm, university of tokyo, were determined on the following items: total protein, albumin, ttt, glucose, bun, total bilirubin, total cholesterol, triglyceride, got, gpt, alp, ldh, cpk, cholinesterase, calcium and inorganic phosphorus. the results obtained were summarized as follows: 1) all items determined were to be analyzed by means of the ... | 1980 | 7202532 |
| defoliation effects on the community structure of arbuscular mycorrhizal fungi based on 18s rdna sequences. | the effects of defoliation on arbuscular mycorrhizal (am) associations in the field were investigated in terms of the community structure of am fungi colonizing roots of grassland plants; the carbohydrate balance of the host plants was also determined. we focused on two plant species dominating japanese native grasslands: the grazing-intolerant species miscanthus sinensis and the grazing-tolerant species zoysia japonica. community structures of am fungi were determined from 18s rrna gene sequenc ... | 2004 | 14727168 |
| [rearing trial of a feed for shiba goats]. | shiba goats are among the widely used for research purposes as laboratory animals and there is an increasing need for development of a suitable feed of reasonably constant nutrient composition and quality in pellet form for them. preliminary studies yielded a pellet feed satisfactorily palatable and adequate as a maintenance ration for this species. the present experiments were conducted to assess the pellet feed for usefulness in rearing shiba goats, in comparison with a conventional ration whi ... | 1981 | 7262200 |
| proposal of sphingomonadaceae fam. nov., consisting of sphingomonas yabuuchi et al. 1990, erythrobacter shiba and shimidu 1982, erythromicrobium yurkov et al. 1994, porphyrobacter fuerst et al. 1993, zymomonas kluyver and van niel 1936, and sandaracinobacter yurkov et al. 1997, with the type genus sphingomonas yabuuchi et al. 1990. | based on the results of phylogenetic analysis of the 16s rdna sequences and the presence of n-2'-hydroxymyristoyl dihydrosphingosine 1-glucuronic acid (sgl-1) and 2-hydroxymyristic acid (non-hydroxymyristic acid in zymomonas) in cellular lipids, a new family, sphingomonadaceae, for group 4 of the alpha-subclass of the class proteobacteria is herein proposed and a description of the family is given. the family consists of six genera, sphingomonas, erythrobacter, erythromicrobium, porphyrobacter, ... | 2000 | 10981829 |
| analysis of transcriptional control elements in the 5'-upstream region of ovine interferon-tau gene using feeder-independent caprine trophoblast cell line, hts-1. | interferon-tau (ifntau) is a protein secreted from the embryonic trophectoderm of ruminant ungulates during peri-implantation period. this protein acts on the uterine endometrium, which indirectly maintains corpus luteum function, and is therefore considered essential for the process of maternal recognition of pregnancy. transcriptional regulation of ifntau genes had been examined using human choriocarcinoma cell lines, jeg-3 or jar, however, molecular mechanisms by which cell and term specific ... | 2004 | 14972449 |
| genetic variability in east asian dogs using microsatellite loci analysis. | an analysis of eight microsatellite loci in 213 animals was performed to define the genetic structure and variability of 11 east asian native dog populations. allele diversity, observed heterozygosities, expected heterozygosities, f-statistics, g(st) estimates, number of migrants per generation (nm), and nei's da distance were calculated. expected mean heterozygosities of asian native dogs varied within a range of 0.310-0.718 with a mean value of 0.580. in a sample of 11 asian dogs, the highest ... | 2001 | 11773246 |
| breed differences in allele frequency of the dopamine receptor d4 gene in dogs. | we previously reported that the dog dopamine receptor d4 (drd4) gene is polymorphic as observed in humans, and four alleles were identified based on the number and/or order of the 12 and 39 bp sequences located in the homologous region of human drd4. to assess the diversity of the drd4 gene in dogs we examined the allelic variations in four breeds (beagle, golden retriever, shetland sheepdog, and shiba) employing the polymerase chain reaction (pcr). as a result, we found three novel alleles and ... | 2001 | 11773252 |
| fasting-induced suppression of pulsatile luteinizing hormone secretion is related to body energy status in ovariectomized goats. | the effect of energy status on the response of luteinizing hormone (lh) pulse frequency to acute short-term energy deficiency created by fasting in estradiol-treated ovariectomized shiba goats was studied in two experiments. in experiment 1, eight goats whose mean body weight (bw) was 25.6 +/- 5.8 (mean +/- s.d.)kg were fed 500 g hay cubes daily for 1 week. then they were fasted for 3 days. blood samples were collected for 4 h at 6 min intervals on the last day of feeding, first, second and thir ... | 2002 | 12137981 |
| cold acclimation improves recovery of cryopreserved grass (zoysia and lolium sp.). | cold acclimation of lolium l. and zoysia willd. grass cultivars significantly increased regrowth of cryopreserved meristems. one wk of cold acclimation improved recovery following cryopreservation but extended acclimation (4-8 wk) resulted in the best regrowth. cold acclimation also significantly increased the dehydration tolerance of both zoysia and lolium meristems. lolium apices cold acclimated for 4 wk produced 60-100% regrowth following cryopreservation by slow freezing or encapsulation-deh ... | 2000 | 12148055 |
| [sewage sludge as fertilizer for grassland]. | the experiment of sewage sludge composted as fertilizer for zoysia japonica was conducted, and the results showed that cd content in soil was lower than 0.3 mg.kg-1 under the condition of the fertilizer application lower than 45 t.hm-2 (dry weight) and the ph value of soil lower than 6.5 (cd as the limiting factor for the amount of fertilizer application). no3(-)-n content of groundwater was lower than the standard of the first class groundwater (10 mg.kg-1). the contents of organic matter, avai ... | 2002 | 12222055 |
| establishment of feeder-independent cloned caprine trophoblast cell line which expresses placental lactogen and interferon tau. | a feeder-independent cloned trophoblast cell line, hts-1, was established from a mature placenta of shiba goat (capra hircus). during the growth phase, single hts-1 cells exhibited ruffled membranes or lamellipodia often accompanied by elongated cell shape, indicating highly motile nature of the cells. at or near confluence, hts-1 cells formed monolayers with few sign of cellular overlapping. binucleate cells were found at a high frequency especially in the peripheral regions of monolayers. in s ... | 2002 | 12361681 |
| [dispensing drugs without prescriptions and the separation of drug dispensaries from the medical practice]. | many cases of dispensing drugs without doctors' prescriptions were reported throughout japan during 1914-16. some cases were brought to the courts. pharmacists had been selling prescription drugs to their customers without prescriptions. this dispensing act was admitted by the ministry of internal affairs after 1913. however, doctors claimed that the act of dispensing drugs without a prescription infringed on law no. 10, and the japan pharmacists association hired a notable lawyer and fought a l ... | 2002 | 12412593 |
| hormonal control of grooming behavior in domestic goats. | in this study, further documentation of the modulation of self-grooming by testosterone was provided by an experiment which showed that self oral grooming in long-term castrated male goats of the dwarf shiba breed was significantly down-regulated to one-third of the baseline grooming rate by testosterone supplementation. observations on grooming in gonadally intact female shiba goats in the summer (july) and in the fall (october) showed that the oral grooming rate was significantly higher in the ... | 2003 | 12536011 |
| turfgrass thatch effects on pesticide leaching: a laboratory and modeling study. | process-based models are frequently used to assess the water quality impacts of turfgrass management emanating from proposed or existing golf courses. thatch complicates the prediction of pesticide transport because surface-applied pesticides must pass through an organic-rich layer before entering the soil. this study was conducted to (i) compare the use of a linear equilibrium model (lem) and two-site nonequilibrium (2sne) model to predict pesticide transport through soil and thatch + soil colu ... | 2003 | 12549561 |
| comparison of polymerase chain reaction-restriction fragment length polymorphism assay and enzyme assay for diagnosis of g(m1)-gangliosidosis in shiba dogs. | in the present study, diagnostic methods for canine g(m1)-gangliosidosis were examined by comparing a dna mutation assay with an enzyme assay. sixty-two shiba dogs of a pedigree with g(m1)-gangliosidosis were differentiated into 3 genotypes, i.e., normal, heterozygous, and homozygous affected dogs, using a dna mutation assay, which consists of polymerase chain reaction amplification and the determination of restriction fragment length polymorphisms. the beta-galactosidase activity in leukocytes, ... | 2004 | 15305740 |
| different effects of subnormal levels of progesterone on the pulsatile and surge mode secretion of luteinizing hormone in ovariectomized goats. | this study tested the hypothesis that endocrinological threshold levels of progesterone that induce negative feedback effects on the pulsatile and surge modes of lh secretion are different. our approach was to examine the effects of subnormal progesterone concentrations on lh secretion. long-term ovariectomized shiba goats that had received implants of silastic capsules containing estradiol were divided into three groups. the high progesterone (high p) group received a subcutaneous implant of a ... | 2003 | 12606364 |
| heredity of red blood cells with high k and low glutathione (hk/lg) and high k and high glutathione (hk/hg) in a family of japanese shiba dogs. | forty-two of 81 dogs from a family of japanese shiba dogs had red blood cells with a high k and a low na concentration (hk). of the hk dogs, 32 were high k and low glutathione (hk/lg) and 10 were high k and high glutathione (hk/hg). these variants were found in both males and females. the phenotype of hk was inherited in a recessive mode as reported earlier. a high incidence of hk/lg dogs was found in this family, and the phenotype was also inherited in a recessive mode. glutamate (glu) influx, ... | 1997 | 9027470 |
| incidence of dogs possessing red blood cells with high k in japan and east asia. | the phenotype of high k (hk) red blood cells, which is an autosomal recessive, was found in dog groups from 10 of 13 breeds or populations in japan. the incidence of hk was 26 to 38% in the san'in-shiba, shinshu-shiba and akita breeds, and the gene frequencies of hk ranged from 0.513 to 0.612. the highest incidence (42%) was found in the jindo breed from korea, and the gene frequency was 0.652. two other groups from korea also possessed this hk variation. however, although hk cells were not foun ... | 1997 | 9234231 |
| the afferent activity of the superior laryngeal nerve, and respiratory reflexes specifically responding to intralaryngeal pressure changes in anesthetized shiba goats. | this study was aimed at characterizing the superior laryngeal nerve (sln) afferent activities under four different respiratory conditions, i.e., tracheostomy breathing (tb), upper airway breathing (uab), tracheal occlusion (to) and upper airway occlusion (uao), and investigating respiratory changes in response to transmural pressures applied to the larynx in anesthetized shiba goats. the activity recorded from the whole sln increased at both inspiration and expiration during tb, uab and to, whil ... | 1997 | 9362036 |
| a clostridial endo-beta-galactosidase that cleaves both blood group a and b glycotopes: the first member of a new glycoside hydrolase family, gh98. | we have isolated an endo-beta-galactosidase designated e-abase from clostridium perfringens atcc 10543 capable of liberating both the a trisaccharide (a-tri; galnacalpha1-->3(fucalpha1-->2)gal) and b trisaccharide (b-tri; galalpha1-->3(fucalpha1-->2)gal) from glycoconjugates containing blood group a and b glycotopes, respectively. we have subsequently cloned the gene (eabc) that encodes e-abase from this organism. this gene was found to be identical to the cpe0329 gene of c. perfringens strain 1 ... | 2004 | 15618227 |
| successful long-term treatment of congenital nephrogenic diabetes insipidus in a dog. | a two-year-old intact male shiba inu dog with excessive polyuria and polydipsia (pu-pd) was diagnosed as having congenital nephrogenic diabetes insipidus based on clinical findings, the results of urinalysis, blood examinations, a modified water deprivation test and a low dose dexamethasone suppression test. the owner was advised to provide adequate access to drinking water, and treatment with a low dose of hydrochlorothiazide (2 mg/kg, twice daily) together with a low sodium diet was initiated. ... | 1998 | 9888114 |
| allelic variation of the d4 dopamine receptor polymorphic region in two dog breeds, golden retriever and shiba. | the d4 dopamine receptor (d4dr) polymorphic region, which is possibly related to the personality trait known as novelty seeking in humans, was examined in 34 dogs from two breeds (golden retriever and the japanese indigenous breed, shiba) by the polymerase chain reaction (pcr), and the dna sequences of each allele were determined. the polymorphic region of the dog d4dr gene was composed of 39- and 12- base pair (bp) units, and four alleles (a-d) were identified based on the number and/or order o ... | 1999 | 10651047 |
| species and cultivar influences on survival and parasitism of fall armyworm. | interactions between host plant resistance and biological control may benefit or hinder pest management efforts. turfgrass cultivars have rarely been tested for extrinsic resistance characteristics such as occurrence and performance of beneficial arthropods on plant genotypes with resistance to known turf pests. parasitism of fall armyworm, spodoptera frugiperda (j.e. smith), among six turfgrass genotypes was evaluated. the six grasses tested [sea isle-1 and 561-79 seashore paspalum, paspalum va ... | 2004 | 15666756 |
| glycoprotein iib-iiia-liposomes bind fibrinogen but do not undergo fibrinogen-mediated aggregation. | although platelet cross-bridging is mediated primarily by the binding of fibrinogen to its activated membrane receptor, glycoprotein (gp) iib-iiia*, such an interaction may not be sufficient to support the aggregation process. as this question could potentially be answered by reconstituting gpiib-iiia* into a non-platelet environment such as liposomes, a protocol was developed for the generation of large lipid vesicles containing purified gpiib-iiia*. flow cytometric techniques confirmed that th ... | 2000 | 10938888 |
| analysis of human abnormal walking using a multi-body model: joint models for abnormal walking and walking aids to reduce compensatory action. | this paper proposes new models of diseased joints and evaluates the effectiveness of walking aids such as a cane and a brace for compensating for lost functions due to joint disorders. the zmj concept described in the previous work (yamashita and tagawa, 1988. in: radharaman (ed.), robotics and factories of the future'87. springer, new york, pp. 670-677) is modified into three joint models as follows: a passive element joint (pej) which has a spring at the diseased joint; a constrained range joi ... | 2000 | 10940399 |
| turfgrass, crop, and weed hosts of blissus occiduus (hemiptera: lygaeidae). | blissus occiduus barber is an important pest of buffalograss, buchloë dactyloides (nuttall) engelmann, turf. no-choice studies documented the susceptibility of selected turfgrasses, crops, and weeds to b. occiduus feeding. highly to moderately susceptible grasses included buffalograss; yellow setaria glauca (l.) and green foxtail setaria viridis (l.); kentucky bluegrass, poa pratensis l.; perennial ryegrass, lolium perenne l.; brome, bromus spp. leyss.; zoysiagrass, zoysia japonica steudel; berm ... | 2004 | 14998128 |
| development of specific pcr primers for the detection of rhizoctonia solani ag 2-2 lp from the leaf sheaths exhibiting large-patch symptom on zoysia grass. | detection of rhizoctonia solani ag 2-2 lp isolates causing large-patch disease on zoysia grass was done using polymerase chain reaction (pcr). specific primers were designed based on an amplified region using random amplified polymorphic dna (rapd)-pcr. fifteen primers and three cultural types of r. solani ag 2-2 (types iiib, iv and lp) were used for rapd-pcr. the banding patterns by rapd-pcr showed that the three cultural types were clearly distinguishable. a dendrogram constructed from the res ... | 2004 | 15019736 |
| the trna-interacting factor p43 associates with mammalian arginyl-trna synthetase but does not modify its trna aminoacylation properties. | arginyl-trna synthetase (argrs) is one of the nine synthetase components of a multienzyme complex containing three auxiliary proteins as well. we previously established that the n-terminal moiety of the auxiliary protein p43 associates with the n-terminal, eukaryotic-specific polypeptide extension of argrs. because p43 is homologous to arc1p, a yeast general rna-binding protein that associates with metrs and glurs and plays the role of trna-binding cofactor in the aminoacylation reaction, we ana ... | 2004 | 15078106 |
| histochemistry of complex carbohydrates in the ceruminous glands of the goat. | the localization and chemical nature of complex carbohydrates in the ceruminous glands of the japanese miniature (shiba) goat were studied using light and electron microscopic histochemical methods, particularly lectin histochemistry. the epithelial cells and luminal secretion of the caprine ceruminous glands contained large amounts of neutral and smaller amounts of acidic glycoconjugates with different terminal sugars (alpha- d-mannose, alpha-l-fucose, alpha-n-acetyl-d-galactosamine, beta-d-gal ... | 2004 | 15083309 |
| body growth and plasma concentrations of metabolites and metabolic hormones during the pubertal period in female shiba goats. | it has been shown in various species that the onset of puberty is closely associated with body growth and nutritional state rather than age. the present study was conducted to determine the timing of puberty and to clarify body growth and metabolic changes around the pubertal period in female shiba goats. blood samples were collected between 10 to 38 weeks of age from 12 female goats, and plasma concentrations of progesterone, metabolites (glucose, nonesterified fatty acid, ketone body and aceti ... | 2004 | 15118247 |
| increased concentration of gm1-ganglioside in cerebrospinal fluid in dogs with gm1- and gm2-gangliosidoses and its clinical application for diagnosis. | gm1- and gm2-gangliosidoses are lethal lysosomal diseases that are caused by a defect of acid hydrolases, resulting in the intralysosomal accumulation of the specific physiological substrates, gm1- and gm2-gangliosides, respectively. in the present study a method for the diagnosis of canine gm1-gangliosidosis was established using canine cerebrospinal fluid (csf). the concentration of gm1-ganglioside in csf was determined by thin-layer chromatography-enzyme immunostaining using biotin-conjugated ... | 2004 | 15152837 |
| glycoconjugate histochemistry of the seminal vesicles of the japanese miniature (shiba) goat. | the present study localizes and characterizes complex glycoconjugates in the seminal vesicles of the japanese shiba goat, using several carbohydrate histochemical procedures, including lectin techniques at light and electron microscopic levels. glandular epithelial cells and luminal secretions were shown to contain neutral and acidic glycoconjugates with various saccharide residues, such as alpha-d-man, alpha-d-glc, alpha-l-fuc, beta-d-gal, galnac, glcnac, and neuac (sialic acid). the terminal o ... | 2004 | 15153071 |
| detection of elements responsible for stage- and tissue-specific expression of mouse sry using an in vitro cre/loxp system. | we have successfully specified essential sequences of the 5' upstream region for the stage- and tissue-specific expression of mouse sry by using an in vitro cre/loxp system. sry/cre plasmids carrying sry 5' sequences of various sizes were transfected into the primary cultured cells from different tissues of cag/loxp/cat/loxp/lacz transgenic fetuses on 11.5-day post coitus (dpc) or 13.5-dpc. stage- and tissue-specific regulation of sry expression was disrupted by the deletion of positions 7549-76 ... | 2005 | 16182245 |
| thymoma in a dog with a part of granular cell proliferation and concurrent lymphoma cells. | a 12-year-old male shiba dog showed anemia and the swelling of systemic lymph nodes. x-ray and post mortal examinations revealed a anterior mediastinal mass. histologically, the tumor mass consisted of four different elements; cord-like proliferation of cuboidal epithelial cells, tubular or cystic structures lined with ciliated epithelial cells, proliferation of large round-shaped epithelial cells with pas-slightly positive granular cytoplasm, and diffuse proliferation of neoplastic lymphocytes. ... | 2004 | 15240949 |
| postnatal development of multivesicular nuclear body in the shiba goat sertoli cell: an ultrastructural study. | the multivesicular nuclear body (mnb) within the sertoli cell nucleolus has been observed in the ruminant testis, but not in other mammalian species. generally, the mnb is composed of vesicles, tubules and ribosome-like structures. this study has been conducted in order to clarify mnb formation during postnatal development. the testes were obtained from immature shiba goats at 1, 2, 3, 4, and 5 months old, and from adults. they were fixed with 5% glutaraldehyde, postfixed with 1% oso4, dehydrate ... | 2004 | 15248561 |
| early pregnancy diagnosis by means of ultrasonography as a method of improving reproductive efficiency in goats. | eighteen cyclic shiba goats were used in this study. estrus was synchronized with a single injection of 125 microg of a synthetic analogue of prostaglandin f(2)alpha (pgf(2)alpha) after detection of at least one corpus luteum by b-mode ultrasonography. blood samples were collected from each animal on days 0, 7 and 21 post-mating for progesterone assay. animals in estrus were either allowed to be mated by fertile bucks twice during estrus (group i; n=12) or not at all (group ii; n=6). ultrasonogr ... | 2004 | 15329470 |