| characterization of tomato zonate spot virus, a new tospovirus in china. | an isolate of a new tospovirus species, causing concentric zoned ringspots on fruits and necrotic lesions on leaves of infected plants, was characterised based on particle morphology, host range and serological properties. the complete nucleotide sequences of large (l), medium (m), and small (s) rnas of this virus were found to contain 8919, 4945, and 3279 nts respectively. the l rna encoded the rna-dependent rna polymerase (rdrp) (2885 aa, 332.7 kda). the m rna encoded a non-structural (nsm) pr ... | 2008 | 18320136 |
| serological comparison and molecular characterization for verification of calla lily chlorotic spot virus as a new tospovirus species belonging to watermelon silver mottle virus serogroup. | abstract calla lily chlorotic spot virus (ccsv) isolated from central taiwan was recently identified as a tospovirus serologically but distantly related to watermelon silver mottle virus (wsmov). to clarify the serological relationship between the two viruses, rabbit polyclonal antibody (pab) to ccsv and mouse monoclonal antibodies (mabs) to wsmov np or ccsv np were produced in this investigation, using purified nucleocapsid protein (np) as immunogens. the pab to ccsv np reacted stronger with th ... | 2005 | 18943560 |
| identification of common epitopes on a conserved region of nss proteins among tospoviruses of watermelon silver mottle virus serogroup. | abstract the nss protein of watermelon silver mottle virus (wsmov) was expressed by a zucchini yellow mosaic virus (zymv) vector in squash. the expressed nss protein with a histidine tag and an additional nia protease cleavage sequence was isolated by ni(2+)-nta resins as a free-form protein and further eluted after sodium dodecyl sulfate-polyacrylamide gel electrophoresis for production of rabbit antiserum and mouse monoclonal antibodies (mabs). the rabbit antiserum strongly reacted with the ns ... | 2006 | 18943661 |
| serological relationship between melon yellow spot virus and watermelon silver mottle virus and differential detection of the two viruses in cucurbits. | melon yellow spot virus (mysv), a tentative member of the genus tospovirus, is considered a distinct serotype due to the lack of a serological relationship with other tospoviruses in its nucleocapsid protein (np). recently, a virus isolate collected from diseased watermelon in central taiwan (mysv-tw) was found to react with a rabbit antiserum (ras) prepared against the np of watermelon silver mottle virus (wsmov), and a monoclonal antibody (mab) prepared against the common epitope of the nss pr ... | 2010 | 20480192 |
| monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in southwestern china. | the thrips-borne tospoviruses calla lily chlorotic spot virus (ccsv), tomato zonate spot virus (tzsv) and a new species provisionally named tomato necrotic spot associated virus (tnsav) infect similar crops in southwestern china. the symptoms exhibiting on virus-infected crops are similar, which is difficult for distinguishing virus species by symptomatology. the sequences of nucleocapsid proteins (nps) of ccsv, tnsav and tzsv share high degrees of amino acid identity with each other, and their ... | 2016 | 27121504 |
| full-length m and l rna sequences of tospovirus isolate 2009-gzt, which causes necrotic ringspot on tomato in china. | the virus isolate 2009-gzt, collected from tomato in guizhou province of china, was identified as a new member of the genus tospovirus based on its s rna sequence. because its provisional name, "tomato necrotic spot virus" (tnsv), was identical to an already existing member of the genus ilarvirus, 2009-gzt was renamed "tomato necrotic spot-associated virus" (tnsav). in this study, the full-length sequences of the genomic m and l rnas of tnsav were determined and analyzed. the m rna has 4,773 nuc ... | 2016 | 26887969 |
| identification of a new tospovirus causing necrotic ringspot on tomato in china. | emerging tospoviruses cause significant yield losses and quality reduction in vegetables, ornamentals, and legumes throughout the world. so far, eight tospoviruses were reported in china. tomato fruits displaying necrotic and concentric ringspot symptoms were found in guizhou province of southwest china. | 2014 | 25465801 |
| molecular characterization of a divergent strain of calla lily chlorotic spot virus infecting celtuce (lactuca sativa var. augustana) in china. | through sequencing and assembly of small rnas, an orthotospovirus was identified from a celtuce plant (lactuca sativa var. augustana) showing vein clearing and chlorotic spots in the zhejiang province of china. the s, m, and l rnas of this orthotospovirus were determined to be 3146, 4734, and 8934 nt, respectively, and shared 30.4-72.5%, 43.4-80.8%, and 29.84-82.9% nucleotide sequence identities with that of known orthotospoviruses. the full length nucleoprotein (n) of this orthotospovirus share ... | 2018 | 29392494 |
| complete genome sequence of a distinct calla lily chlorotic spot virus isolated in mainland china. | the first complete genome sequence of calla lily chlorotic spot virus (ccsv) from lijiang in northwestern yunnan province was obtained using rt-pcr with designed primers. the genome of ccsv isolate lj-1-yunnan is tripartite. the small (s) rna is 3182 nucleotides (nt) in length and encodes a nonstructural protein (nss, 1383 nt) and a nuclear nucleocapsid (n, 834 nt), separated by an 836-nt intergenic region (igr). the medium (m) rna is 4749 nt in length and encodes a nonstructural movement protei ... | 2016 | 26497175 |