evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ...200716822636
a convenient microbiological assay employing cell-free extracts for the rapid characterization of gram-negative carbapenemase producers.the dissemination of metallo and serine carbapenem-hydrolysing beta-lactamases among gram-negative nosocomial bacteria represents an acute problem worldwide. here, we present a rapid and sensitive assay for the characterization of carbapenemase producers to aid in infection control and prevention.200818456651
transstadial and horizontal transfer of bacteria within a colony of anopheles gambiae (diptera: culicidae) and oviposition response to bacteria-containing a paratransgenic approach, genetically modified bacteria are utilized to kill the parasite in the vector gut. a critical component for paratransgenics against malaria is how transgenic bacteria can be introduced and then kept in a mosquito population. here, we investigated transstadial and horizontal transfer of bacteria within an anopheles gambiae mosquito colony with the focus on spiked breeding sites as a possible means of introducing bacteria to mosquitoes. a pantoea stewartii strain, pre ...200818671931
sepsis caused by elizabethkingia miricola successfully treated with tigecycline and levofloxacin.elizabethkingia miricola is a gram-negative rod that was initially isolated from condensation water of the space station mir. this is the 1st reported case of human disease caused by this organism.200818842380
genetic diversity of chromosomal metallo-beta-lactamase genes in clinical isolates of elizabethkingia meningoseptica from korea.this study was performed to characterize the chromosomal metallo-beta-lactamases (mbls) of elizabethkingia meningoseptica isolated from korea and to propose a clustering method of blab and gob mbls based on their amino acid similarities. chromosomal mbl genes were amplified by pcr from 31 clinical isolates of e. meningoseptica. these pcr products were then cloned into a vector and electrotransformed into e. coli dh5 alpha. nucleotide sequencing was performed by the dideoxy chain termination meth ...201020571954
in vitro antifungal and antibacterial activities of pentacycloundecane tetra-amines.the antifungal and antimicrobial activities of three pentacycloundecane (pcu) tetra-amine derivatives are reported herein. the in vitro activity of these pcu derivatives against yeasts (candida albicans and non-albicans species) and filamentous fungi was evaluated using the clinical and laboratory standards institute (clsi) m27-a2 and m38-a2 guidelines and the 2h-tetrazolium salt, (mts) colorimetric method. the minimum inhibitory concentration against most of the tested clinical fungal strains f ...201121241455
biofilm formation and adherence characteristics of an elizabethkingia meningoseptica isolate from oreochromis mossambicus.abstract: background: elizabethkingia spp. are opportunistic pathogens often found associated with intravascular device-related bacteraemias and ventilator-associated pneumonia. their ability to exist as biofilm structures has been alluded to but not extensively investigated. methods: the ability of elizabethkingia meningoseptica isolate ch2b from freshwater tilapia (oreochromis mossambicus) and e. meningoseptica strain nctc 10016t to adhere to abiotic surfaces was investigated using microtiter ...201121545730
catalytic role of the metal ion in the metallo-beta-lactamase gob.metallo-beta-lactamases (mbetals) stand as one of the main mechanisms of bacterial resistance toward carbapenems. the rational design of an inhibitor for mbetals has been limited by an incomplete knowledge of their catalytic mechanism and by the structural diversity of their active sites. here we show that the mbetal gob from elizabethkingia meningoseptica is active as a monometallic enzyme by using different divalent transition metal ions as surrogates of the native zn(ii) ion. of the metal der ...201020007696
clinical and microbiological analysis of elizabethkingia meningoseptica bacteremia in adult patients in taiwan.a limited number of reports have documented bacteremia caused by elizabethkingia meningoseptica (chryseobacterium meningosepticum) in adults. most cases have occurred in taiwan. this study investigated the clinical features and risk factors for mortality from e. meningoseptica bacteremia and the antimicrobial susceptibilities of the isolates. patients with e. meningoseptica bacteremia were retrospectively analyzed at a medical center/teaching hospital in northern taiwan over a 3-y period. we ana ...200919579148
first isolation and identification of elizabethkingia meningoseptica from cultured tiger frog, rana tigerina rugulosa.elizabethkingia meningoseptica has been recognised as an occasional but serious opportunistic bacterial pathogen to human beings. recently, it was frequently isolated from tiger frog, rana tigerina rugulosa, with cataract disease, which is the most common disease of unknown aetiology of frogs in hainan, china. the purpose of this study was to identify and characterise the bacterial strains isolated from the recent outbreaks of cataract disease in farmed tiger frog in hainan, china, and to evalua ...200919327918
the metallo-beta-lactamase gob is a mono-zn(ii) enzyme with a novel active site.metallo-beta-lactamases (mbetals) are zinc-dependent enzymes able to hydrolyze and inactivate most beta-lactam antibiotics. the large diversity of active site structures and metal content among mbetals from different sources has limited the design of a pan-mbetal inhibitor. here we report the biochemical and biophysical characterization of a novel mbetal, gob-18, from a clinical isolate of a gram-negative opportunistic pathogen, elizabethkingia meningoseptica. different spectroscopic techniques, ...200717403673
molecular and biochemical heterogeneity of class b carbapenem-hydrolyzing beta-lactamases in chryseobacterium meningosepticum.although the carbapenem-hydrolyzing beta-lactamase (chbetal) blab-1 is known to be in chryseobacterium meningosepticum nctc 10585, a second chbetal gene, bla(gob-1), was cloned from another c. meningosepticum clinical isolate (pint). the g+c content of bla(gob-1) (36%) indicated the likely chromosomal origin of this gene. its expression in escherichia coli dh10b yields a mature chbetal with a pi of 8.7 and a relative molecular mass of 28.2 kda. in e. coli, gob-1 conferred resistance to narrow-sp ...200010858348
polyphasic study of chryseobacterium strains isolated from diseased aquatic animals.members of most chryseobacterium species occur in aquatic environments or food products, while strains of some other species are pathogenic to humans and animals. a collection of 52 chryseobacterium sp. strains isolated from diseased fish, one frog isolate and 22 reference strains were included in a polyphasic taxonomy study. fourteen clusters of strains were delineated following the comparison of whole-cell protein profiles. most of these clusters were confirmed when the phenotypic and rapd pro ...200516156122
an outbreak of elizabethkingia meningoseptica neonatal meningitis in mauritius.elizabethkingia meningoseptica is a rare but well-recognised cause of neonatal meningitis. reported outbreaks have involved very few cases. we describe the management and outcome of a relatively large outbreak of e.meningoseptica neonatal meningitis201122169781
elizabethkingia meningosepticum : an emerging cause of septicemia in critically iii patients. 201121701671
broad antibiotic resistance profile of the subclass b3 metallo-ß-lactamase gob-1, a di-zinc enzyme.the metallo-ß-lactamase (mbl) gob-1 was expressed via a t7 expression system in escherichia coli bl21(de3). the mbl was purified to homogeneity and shown to exhibit a broad substrate profile, hydrolyzing all the tested ß-lactam compounds efficiently. the gob enzymes are unique among mbls due to the presence of a glutamine residue at position 116, a zinc-binding residue in all known class b1 and b3 mbl structures. here we produced and studied the q116a, q116n and q116h mutants. the substrate prof ...201121299838
transmission of elizabethkingia meningoseptica (formerly chryseobacterium meningosepticum) to tissue-allograft recipients: a report of two cases. 201020516326
atp modulates the growth of specific microbial strains.the regulatory function of extracellular atp (exatp) in bacteria is unknown, but recent studies have demonstrated exatp induced enhanced secondary metabolite production and morphological differentiation in streptomyces coelicolor. the growth of streptomyces coelicolor, however, was unaffected by exatp, although changes in growth are common phenotypes. to identify bacteria whose growth is altered by exatp, we measured exatp-induced population changes in fast-growing microbes and actinomycetes in ...201120512647
acute emergence of elizabethkingia meningoseptica infection among mechanically ventilated patients in a long-term acute care describe an outbreak of infection associated with an infrequently implicated pathogen, elizabethkingia meningoseptica, in an increasingly prominent setting for health care of severely ill patients, the long-term acute care hospital.201019929372
soonwooa buanensis gen. nov., sp. nov., a member of the family flavobacteriaceae isolated from seawater.a gram-negative, non-motile, non-gliding, strictly aerobic, pale yellow colony-forming, rod-shaped bacterial strain, designated hm0024(t), was isolated from coastal seawater of the yellow sea and subjected to a polyphasic taxonomy study. based on 16s rrna gene sequence similarities, strain hm0024(t) was most closely related to chryseobacterium balustinum lmg 8329(t) (94.4 %), chryseobacterium scophthalmum lmg 13028(t) (94.4 %), chryseobacterium piscium lmg 23089(t) (94.3 %) and elizabethkingia m ...201019819990
planobacterium taklimakanense gen. nov., sp. nov., a member of the family flavobacteriaceae that exhibits swimming motility, isolated from desert soil.a gram-negative and rod-shaped bacterial strain, x-65(t), isolated from desert soil of xinjiang, china, was subjected to phenotypic and phylogenetic studies. on the basis of 16s rrna gene sequence analyses, the isolate showed closest relationship to members of the genera chryseobacterium (92.4-95.4 % similarity), cloacibacterium (94.0 %), sejongia (93.9 %) and elizabethkingia (93.8 %) and to riemerella columbina lmg 11607(t) (93.3 %). the strain differed from its phylogenetic neighbours by its s ...200919542118
oleate hydratase catalyzes the hydration of a nonactivated carbon-carbon bond.the hydration of oleic acid into 10-hydroxystearic acid was originally described for a pseudomonas cell extract almost half a century ago. in the intervening years, the enzyme has never been characterized in any detail. we report here the isolation and characterization of oleate hydratase (ec from elizabethkingia meningoseptica.200919465645
secretion of gob metallo-beta-lactamase in escherichia coli depends strictly on the cooperation between the cytoplasmic dnak chaperone system and the sec machinery: completion of folding and zn(ii) ion acquisition occur in the bacterial periplasm.metallo-beta-lactamases (mbetals) are zinc-dependent enzymes produced by many clinically relevant gram-negative pathogens that can hydrolyze most beta-lactam antibiotics. mbetals are synthesized in the bacterial cytoplasm as precursors and are secreted into the periplasm. here, we report that the biogenesis process of the recently characterized mbetal gob-18 demands cooperation between a main chaperone system of the bacterial cytoplasm, dnak, and the sec secretion machinery. using the expression ...200919433552
transfer of chryseobacterium meningosepticum and chryseobacterium miricola to elizabethkingia gen. nov. as elizabethkingia meningoseptica comb. nov. and elizabethkingia miricola comb. nov.the taxonomic positions of six strains (including the type strain) of chryseobacterium meningosepticum (king 1959) vandamme et al. 1994 and the type strain of chryseobacterium miricola li et al. 2004 were re-evaluated by using a polyphasic taxonomic approach. phylogenetic analysis, based on 16s rrna gene sequencing, showed that the strains represent a separate lineage from the type strains of the chryseobacterium-bergeyella-riemerella branch within the family flavobacteriaceae (90.7-93.9 % simil ...200515879269
elizabethkingia meningosepticum (chryseobacterium meningosepticum) infections in children.chryseobacterium meningosepticum is a ubiquitous gram-negative bacillus historically associated primarily with meningitis in neonates and a wide variety of infections in immunocompromised patients. neonatal infections often occur as outbreaks with environmental contamination being the source. c. meningosepticum infections are not common but are clinically important because the organism is naturally resistant to multiple antibiotics. in this paper, we have reviewed the nosocomial outbreaks of c. ...201122046191
neonatal meningitis caused by elizabethkingia meningoseptica in saudi arabia.elizabethkingia meningoseptica is a multi-drug resistant organism that can cause meningitis in premature neonates. we report a case of elizabethkingia meningoseptica meningitis that was detected early in an extremely premature low birth weight infant. he was successfully treated with a combination of ciprofloxacin and piperacillin-tazobactam. the spread of infection was controlled with no other reported cases.201121997946
elizabethkingia anophelis sp. nov., isolated from the midgut of anopheles gambiae.a slightly yellow pigmented bacterial strain (r26t), isolated from the midgut of anopheles gambiae was studied for its taxonomic allocation and growth rate and antibiotic resistance properties. the isolate produced rod shaped cells, which stained gram-negative. the bacterium had two growth optima at 30°c and 37°c, respectively. strain r26t demonstrated natural antibiotic resistance to ampicillin, chloramphenicol, kanamycin, streptomycin and tetracyclin. a comparison of the 16s rrna gene sequence ...201021169462
[the midgut bacterial flora in lab-reared anopheles sinensis].to screen gram-negative bacteria in the midguts of lab-reared anopheles sinensis at 3 development stages (larva, unfed adult and engorged adult).201020666322
clinical features, antimicrobial susceptibilities, and outcomes of elizabethkingia meningoseptica (chryseobacterium meningosepticum) bacteremia at a medical center in taiwan, 1999-2006.a total of 118 patients with elizabethkingia meningoseptica bacteremia at a medical center in taiwan from 1999 to 2006 were studied. minimum inhibitory concentrations (mics) of 99 preserved isolates were determined. the incidence (per 100,000 admissions) of e. meningoseptica bacteremia increased from 7.5 in 1996 to 35.6 in 2006 (p?=?0.006). among them, 84% presented with fever, 86% had nosocomial infections, and 60% had acquired the infection in intensive care units (icus). the most common under ...201121461847
self-induced elizabethkingia meningoseptica endophthalmitis: a case report.abstract:201121745367
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