| isolation of ustilago bromivora strains from infected spikelets through spore recovery and germination. | ustilago bromivora is a biotrophic smut fungus infecting brachypodium sp. it is closely related to the barley-infecting smut ustilago hordei, and related to the well-studied, gall-inducing model pathogen ustilago maydis. upon flowering, the spikelets of u. bromivora-infected plants are filled with black fungal spores. while it is possible to directly use this spore material to infect brachypodium seeds, in many cases it is more useful to isolate individual strains of u. bromivora for a genetical ... | 2017 | 28781989 |
| a complete toolset for the study of ustilago bromivora and brachypodium sp. as a fungal-temperate grass pathosystem. | due to their economic relevance, the study of plant pathogen interactions is of importance. however, elucidating these interactions and their underlying molecular mechanisms remains challenging since both host and pathogen need to be fully genetically accessible organisms. here we present milestones in the establishment of a new biotrophic model pathosystem: ustilago bromivora and brachypodium sp. we provide a complete toolset, including an annotated fungal genome and methods for genetic manipul ... | 2016 | 27835569 |