| a one-step multiplex rt-pcr assay for simultaneous detection of four viruses that infect peach. | a multiplex reverse transcription polymerase chain reaction (mrt-pcr) assay was developed to enable the simultaneous detection and differentiation of four viruses that infect peach, namely apple chlorotic leaf spot virus (aclsv), cherry green ring mottle virus (cgrmv), prunus necrotic ringspot virus (pnrsv) and apricot pseudo-chlorotic leaf spot virus (apclsv). in this study, four pairs of primers, one specific for each virus, were designed; the corresponding pcr products were 632, 439, 346 and ... | 2013 | 23777367 |
| molecular variability analyses of apple chlorotic leaf spot virus capsid protein. | the complete sequences of the coat protein (cp) gene of 26 isolates of apple chlorotic leaf spot virus (aclsv) from india were determined. the isolates were obtained from various pome (apple, pear and quince) and stone (plum, peach, apricot, almond and wild himalayan cherry) fruit trees. other previously characterized aclsv isolates and trichoviruses were used for comparative analysis. indian aclsv isolates among themselves and with isolates from elsewhere in the world shared 91-100% and 70-98% ... | 2010 | 21289443 |
| characterization of apricot pseudo-chlorotic leaf spot virus, a novel trichovirus isolated from stone fruit trees. | abstract a trichovirus closely related to apple chlorotic leaf spot virus (aclsv) was detected in symptomatic apricot and japanese plum from italy. the sus2 isolate of this agent cross-reacted with anti-aclsv polyclonal reagents but was not detected by broad-specificity anti- aclsv monoclonal antibodies. it had particles with typical trichovirus morphology but, contrary to aclsv, was unable to infect chenopodium quinoa and c. amaranticolor. the sequence of its genome (7,494 nucleotides [nt], mis ... | 2005 | 18943045 |