| characterization of beta-adrenergic receptor linked to adenylate cyclase in a human cancer cell line (colo 16). | 1. a human cancer cell line (colo 16) derived originally from an epidermal squamous cell carcinoma was found to possess adenylate cyclase responsiveness to beta-adrenergic agonists. 2. the adenylate cyclase response was characterized with respect to activation constants (ka) for various beta-adrenergic agonists and inhibition constants (ki) for antagonists. 3. intact cells responded with dose-dependent increases in production of cyclic adenosine 3':5'-monophosphate. 4. properties of the beta-adr ... | 1978 | 27327 |
| in vitro immunization against human tumor cells with tumor cell fractions. | tumor cell fractions isolated from tumor lines sh-3 (breast carcinoma) and rpmi-7932 (malignant melanoma) by differential centrifugations were capable of transforming lymphocytes into cytotoxic effector cells. lymphocytes cultured alone in human ab plasma did not become cytotoxic to tumor cells. however, when cultured with tumor cell fractions sedimented at 1000 x g(r1), 20,000 x g(r2), and 100,000 x g(r3), these lymphocytes became markedly cytotoxic to specific tumor targets in a 3.5-hr (51)cr ... | 1977 | 72600 |
| phenotypic and epidemiologic correlates of auxotype in neisseria gonorrhoeae. | previous studies from seattle, wash., suggested that strains of neisseria gonorrhoeae which require arginine, hypoxanthine, and uracil (arg-hyx-ura- auxotype) are unifomly highly susceptible to penicillin g, are relatively resistant to complement-dependent killing by heated, pooled human serum, and are associated with disseminated gonococcal infection. for further study of the epidemiology of these strains and for analysis of the susceptibility to penicillin, serum sensitivity, and the nutrition ... | 1978 | 98600 |
| [colo-pyloric anastomosis with pyloroplasty in esophagoplasty]. | a procedure is presented, allowing to include the stomach in the intestinal pathway in cases of oesophagoplasties, preventing at the same time the development of anastomotic peptic ulcers. a colo-pyloric anastomosis is performed with the aboral stump of the elevated colic loop. the colic end is anastomosed terminoterminally to the pylorus, in such a way that a 6--7 cm pylorotomy is performed. the pyloric function is suppressed and the evacuation of the stomach's contents into the duodenum is fac ... | 1977 | 143683 |
| [immunologic results obtained with an experimental system oftransplantable colonic tumors. possible applications of these results to human intestinal tumors]. | intestinal cancers, morphologically very close to human colo-rectal adeno-carcinoma, have been induced by dimethylhydrazine in inbred rats. graftable lines have been obtained from 5 primary intestinal tumors, and 3 cell lines have been cultivated from the grafts. this model was used to demonstrate carcinofetal antigen(s) on the surface of the intestinal cancer cells. circulating antibodies against tumor-associated antigen(s) have been found in tumor-bearing rats. cancer enhancement was regularly ... | 1975 | 174767 |
| [morphological and enzymatic studies of the supratumoral distended colonic loop in colorectal cancers (therapeutic aspects)]. | the authors carried out experimental and clinical studies in an attempt to explain the causes leading to anastomotic dehiscences, a major complication of exeresis surgery of the left colon and of the rectum. in this view they have performed in dogs incomplete stenoses at the level of the sigmoid and also made parallel studies of the morphological and histochemical changes occuring in the supra-stenosis colon wall, with those of the supra-tumoral colon wall found in human colo-rectal carcinomas, ... | 1975 | 175406 |
| editorial: chihuahuas and laetrile, chelation therapy, and honey from boulder, colo. | | 1976 | 182097 |
| a transitional cell carcinoma cell line. | a transitional cell carcinoma cell line, colo 232, was derived from a primary urinary bladder tumor in a caucasian male. in culture, colo 232 retained distinct uroepithelial phenotypic traits and produced both carcinoembryonic antigen and adrenocorticotropic hormone. colo 232 had a chromosome mode of 58 and retained the x and y chromosomes. ten marker chromosomes were identified. colo 232 will be of value for biochemical and immunological studies. | 1978 | 208962 |
| electron microscopic studies on transplantable mucus-secreting and tubular adenocarcinomas of colo-rectal origin in aci/n rats. | two transplantable, one mucus-producing (r-1) and the other tubular but less mucinous (r-2), adenocarcinomas were investigated electron microscopically. the r-1 tumor was composed of a large number of intermediate cells and mucus-producing cells, incompletely differentiated goblet-like cells and absorptive-like cells, and a small number of undifferentiated cells. the electron microscopic features of the mucus-producing cells exhibited distinctive features different from those of the epithelium o ... | 1979 | 220752 |
| experimental colo-rectal tumours in the rat. a histopathologic study with special reference to normal anatomy and to immunologic and radiographic aspects. | | 1978 | 278472 |
| effective adjuvant treatments. a brief review of u.s. clinical trials. | a variety of adjuvant trials performed in the united states for osteogenic sarcoma, breast, lung, and colon cancer have achieved encouraging results and are briefly summarized. trials in osteogenic sarcoma are reporting 2-year disease-free survival rates of 50%. however, they have only been evaluated against historical controls and there is some evidence that other factors might have greatly improved the disease-free survival in the absence of adjuvant therapy. the nsabp breast cancer trial only ... | 1978 | 282826 |
| marker properties of tumor and lymphoid cell lines derived from a patient with squamous cell carcinoma. | a squamous cell carcinoma tumor cell line, colo 227, derived from a metastatic tumor in a caucasian male, produces both parathyroid hormone and carcinoembryonic antigen. a chromosome mode of 106 predominated and the x and y chromosomes were retained. seven marker chromosomes were identified. cytogenetic analysis revealed an isochromosome 8 marker similar to a hela cell line marker and an isochromosome 17 marker described in other cancers. an autochthonous lymphoid cell line, colo 219, was establ ... | 1977 | 301054 |
| flow-cytometric demonstration of tumour-cell subpopulations with different dna content in human colo-rectal carcinoma. | | 1979 | 436903 |
| antibody-dependent cellular cytotoxicity to human colon-tumour cells. i. lack of tumour specificity in a population study. | | 1979 | 475969 |
| comparison of four new cell lines from patients with adenocarcinoma of the ovary. | permanent human tumor cell lines colo 110, colo 316, colo 319, and colo 330 were established from four patients with serous cystadenocarcinoma of the ovary. colo 110 was derived from primary tumor tissue; colo 316, colo 319, and colo 330 were derived from cells in malignant effusions. colo 110 and colo 316 grew as monolayers of epithelioid cells in culture; colo 319 and colo 330 grew as vermiform, floating colonies of epithelioid cells in culture. epithelial-like morphology was confirmed by tran ... | 1979 | 498076 |
| cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. | two human colon carcinoma cell lines derived from the same tumor specimen were characterized. the cell lines, colo 320 and colo 321, have amine precursor uptake and decarboxylation cell properties, such as ectopic production of norepinephrine, epinephrine, serotonin, adrenocorticotropic hormone, and parathyroid hormone. the cells were morphologically different from most colon cell lines. double minutes (dm) were initially present in nearly 100% of the metaphases. in a few subcultures of colo 320 ... | 1979 | 498117 |
| bovine mammary gland contains a growth promoter for human breast cancer cell lines: colo 378 and colo 384. | | 1979 | 518590 |
| reflex pathways in the abdominal prevertebral ganglia: evidence for a colo-colonic inhibitory reflex. | 1. in vitro experiments were performed on preparations consisting of prevertebral ganglia attached to the entire colon of guinea-pigs. the colon was divided into an orad and a caudad segment and intraluminal pressure was recorded from the terminal end of each segment. intracellular recordings were simultaneously obtained from neurones in the coeliac plexus. 2. the source of mechanosensory input from the colon paralleled the responses to mesenteric nerve stimulation. that is, section of the mesen ... | 1979 | 521925 |
| colo-rectal biopsy in inflammatory bowel disease. | the term inflammatory bowel disease (ibd) is viewed as all-inclusive, covering the full panoply of intestinal disorders in which inflammatory changes are a prominent feature, including those of infectious, toxic, and intrinsic origin as well as the idiopathic entities ulcerative colitis and crohn's disease. this chapter describes and discusses those aspects of colo-rectal biopsy in ibd which can help pathologists make optimal interpretations. the areas covered are: 1) methods used to prepare bio ... | 1977 | 611415 |
| [the colo-rectal carcinoma. studies on epidemiology and animal experimental carcinogenesis]. | | 1978 | 620967 |
| variation in response of xenografts of colo-rectal carcinoma to chemotherapy. | ten xenograft lines of human colonic and rectal carcinomas have been established in immune-suppressed mice. mice bearing tumours in the 2nd to 6th passage were treated with maximum tolerated doses of 8 chemotherapeutic agents and tumour growth delay was estimated in terms of the number of volume doubling times gained by the treatment. the average response corresponded to a delay of only about 0.5 doubling times, but some tumour lines showed a good response to some agents. twenty-three out of abo ... | 1978 | 646928 |
| variations in sialomucins in the mucosa of the large intestine in malignancy: a quantimet and statistical analysis. | the periodate-borohydride/saponification/pas (pb/koh/pas) method, for the identification of sialic acid derivatives, was used to investigate possible changes in human colonic epithelium associated with carcinogenesis. the material was obtained from specimens of the large intestine resected for carcinoma and compared with normal control mucosa from biopsies of patients with no known gastro-intestinal diseases, using a quantimet image analyser densitometer to measure these staining reactions. an o ... | 1978 | 689911 |
| bacterial density in laparotomy wounds during gastro-instestinal operations. | operation wounds were sampled quantitatively for aerobic bacteria in 56 patients by the velvet pad rinse technique in a prospective, randomised study of abdominal operations (1) truncal vagotomy and pyloroplasty, (2) gastric or (3) colo-rectal operations, and (4) exploratory laparotomy. on entering the peritoneal cavity and prior to incision of the gastro-intestinal tract the bacterial density in the wounds did not differ significantly between these four types of operations, the median being 5.4 ... | 1976 | 775616 |
| binding of ige myeloma proteins to human cultured lymphoblastoid cells. | the binding of human ige myeloma proteins to 16 human cultured lymphoblastoid cell lines was studied by measuring specific uptake of radiolabeled deaggregated ige myeloma proteins and/or e-ige rosette formation. eight lines, rpmi-8866, wil-2wt, rpmi-6410, rpmi-1788, rpmi-4265, clowers, colo-59 and victor, bound ige as shown by at least one of these methods. the lines, rpmi-4098, scrf-5004, nc-37, daudi, raji, p3jhr-1, rpmi-1301 and molt-4 did not bind ige. of the positive cell lines, 58 to 98% o ... | 1976 | 792332 |
| [experimental research on everting intestinal sutures. ii. colo-colic anastomosis]. | | 1976 | 800362 |
| a critical evaluation of the carcinogenic role of amoebiasis with special reference to a case of double primary malignancies. | the case of a 45 year old man with double primary malignancies of unrelated organs--namely, amoebiasis-associated colo-rectal and renal cell carcinomas, is reported. the problem of carcinogenic role of amoebiasis in particular and that of parasitic diseases in general, is discussed and the literature briefly reviewed. | 1976 | 829727 |
| [appendectomy and colo-rectal carcinoma. clinico-statistical and experimental studies]. | a clinico-statistical investigation has been carried out with a view to establishing whether or not there is any correlation between appendicectomy and onset of colorectal cancer. the analysis showed that the frequencies of appendicectomy in the sample studied and in the control sample were not statistically different. on the other hand, it was observed that in patients suffering from colorectal carcinoma, the age at which appendicectomy was performed is is greater than in control subjects, with ... | 1977 | 857199 |
| cell and organ culture techniques applied to the study of carcinoma of colon and rectum. | the value of a number of in vitro systems which have been used in the investigation of carcinomas of colon and rectum is considered. tissue culture cell ines which have been established from human and mouse rectal and colon tumours are described. both mouse and human cells retain ultrastructural features of the tumours of origin; acinus formations, junctional complexes, microvilli with surface glycoprotein strands. normal colo-rectal epithelium has not been established in cell culture but organ ... | 1976 | 980543 |
| proceedings: the importance of bacteroides as a cause of severe sepsis after colo-rectal surgery. | | 1975 | 1140690 |
| a human squamous cell carcinoma cell line. | an epithelial cell line colo 16 has been established from a human squamous carcinoma, characterized and maintained for over two years. the cells produce a parathyroid-like hormone and carcinoembryonic antigen. the line is definitely not a "hela contaminant." the cell line is available to other investigators. | 1975 | 1157043 |
| [relationship between neuronal activities of the guinea-pig inferior mesenteric ganglion neurons and muscular contraction of the colon in vitro]. | a perfused inferior mesenteric ganglion (img)-colon preparation was prepared from the guinea-pig for intracellular recording of img neurons and for simultaneously recording the muscular contraction. it was found that: (1) at zero preloaded tension of colon, the spontaneous fast excitatory post-synaptic potentials (f-epsps) could be recorded in about 50% sampled neurons. these f-epsps were abolished by either severing the colonic nerve or superfusing img with d-tubocurarine (d-tc, 50 mumol/l). (2 ... | 1992 | 1293747 |
| demonstration of monoclonal anti-carcinoembryonic antigen (cea) antibody internalization by electron microscopy, western blotting and radioimmunoassay. | one of the important factors affecting the action of monoclonal antibodies (mabs) or immunoconjugates on tumour sites depends on whether the mab is internalized by the cancer cells in question. the underexplored subject of internalization is discussed in this paper, and a number of in vitro techniques for investigating internalization are evaluated, using a model which consists of a well characterized anti-carcinoembryonic antigen (anti-cea) mab and a number of cea expressing human cancer cell l ... | 1992 | 1295459 |
| multiple antigens recognized by anti-c-myc antibodies in human cells and xenopus oocytes. | we have investigated the localization, solubility, serum regulation, and phosphorylation of myc antigens from colo 320 cells, a human transformed cell line with an amplified c-myc gene, and from xenopus oocytes, which express high levels of c-myc mrna. although myc proteins are often reported to range from 60 to 68 kilodaltons, our panel of anti-myc monoclonal antibodies recognized a number of higher and lower molecular mass antigens, in addition to proteins within this range. based upon various ... | 1992 | 1297358 |
| convergence of visceral and somatic inputs onto subnucleus reticularis dorsalis neurones in the rat medulla. | 1. in anaesthetized rats, recordings were made within the medullary subnucleus reticularis dorsalis (srd) from neurones that exhibited convergence of nociceptive inputs from the entire surface of the body. neurones with total nociceptive convergence responded to supramaximal percutaneous electrical stimuli (2 ms duration) with early and late peaks due to the activation of a delta and c fibres, respectively, no matter which part of the body was stimulated. neurones with partial nociceptive conver ... | 1992 | 1302266 |
| determination of cyp1a2 and nat2 phenotypes in human populations by analysis of caffeine urinary metabolites. | the wide variations in urinary bladder and colo-rectal cancer incidence in humans have been attributed in part to metabolic factors associated with exposure to carcinogenic aromatic and heterocyclic amines. cytochrome p-4501a2 (cyp1a2), which catalyses n-oxidation, and acetyltransferase (nat2) which catalyses n- and o-acetylation, both appear to be polymorphically distributed in human populations; and slow and rapid nat2 phenotypes have been implicated as risk factors for these cancers. caffeine ... | 1992 | 1306111 |
| characterization of insulinlike growth factor i receptors in human colon cancer. | a possible role of insulinlike growth factor i (igf-i) and igf-i receptors in the proliferation of human colon cancer has, to the best of the authors' knowledge, not been examined as yet. to determine a role of igf-i in colon cancer, several human colon cancer cell lines and colon cancers were screened for specific binding to 125i-igf-i. almost all the human colon cancer cells examined were variably positive for specifically binding 125i-igf-i. almost half the colon cancer cell lines examined sh ... | 1992 | 1312970 |
| c-myc and c-ha-ras cellular oncogenes and human papillomaviruses in benign and malignant cutaneous lesions. | to analyze the role of human papillomavirus (hpv) infection and c-myc and c-ha-ras oncogene activation in cutaneous and mucosal lesions, serial frozen sections of 47 lesions from grafted recipients and 10 biopsies from non-immunosuppressed patients were examined. hela, caski, mcf7, colo 320 and 3t3 cells, which contain various copy numbers of hpv dna and/or c-myc gene, were used as controls. hpv, myc and ras oncogene dnas were not detected in normal epithelia by in situ hybridization with biotin ... | 1992 | 1317208 |
| inhibition of the activation of heat shock factor in vivo and in vitro by flavonoids. | transcriptional activation of human heat shock protein (hsp) genes by heat shock or other stresses is regulated by the activation of a heat shock factor (hsf). activated hsf posttranslationally acquires dna-binding ability. we previously reported that quercetin and some other flavonoids inhibited the induction of hsps in hela and colo 320dm cells, derived from a human colon cancer, at the level of mrna accumulation. in this study, we examined the effects of quercetin on the induction of hsp70 pr ... | 1992 | 1321338 |
| effects of a new triazinoaminopiperidine derivative on adriamycin accumulation and retention in cells displaying p-glycoprotein-mediated multidrug resistance. | a new triazinoaminopiperidine derivative, servier 9788 (s9788), was investigated for its ability to increase adriamycin (adr) accumulation and retention in two rodent (p388/adr and dc-3f/ad) and three human (kb-a1, k562/r and colo 320dm) cell lines displaying the p-glycoprotein (p-gp)-mediated multidrug resistance (mdr) phenotype. depending on the cell line s9788 was shown to be two to five times more active and five to 15 times more potent than verapamil (vrp) in increasing adr accumulation in ... | 1992 | 1360210 |
| identification and characterization of a partial cdna expressing interleukin-1-like activity in keratinocytes. | a partial cdna has been isolated from the human keratinocyte cell line colo-16 which is distinct from either il-1 alpha or beta and encodes a protein which displays some of the biological properties associated with il-1. the 720 bp partial cdna hybridized on northern blots of colo-16 mrna to a 1.6 kbp message of low abundance. expression of the partial cdna in cos-1 cells resulted in activity in three il-1 assays: thymocyte co-stimulation, d10.g4.1 t-cell stimulation and fibroblast proliferation ... | 1992 | 1372959 |
| in vivo circumvention of human colon carcinoma resistance to bleomycin. | metabolic inactivation of bleomycin (blm) by cysteine proteinase-like enzymes is thought to be a major mechanism of blm tumor resistance. we now report that the human colon carcinoma colo-205 is highly resistant to blm and that e-64, a cysteine proteinase inhibitor, sensitizes colo-205 to blm. treatment of colo-205-bearing nude mice with either e-64 (40 mg/kg) or blm (10 mg/kg) alone did not inhibit colo-205 growth. however, pretreatment with e-64 prior to blm prevented these xenografts from gro ... | 1992 | 1374681 |
| characterization of an endogenous rna transcript with homology to the antisense strand of the human c-myc gene. | in addition to being regulated by a complex array of cis- and trans-acting factors, c-myc protooncogene expression may be modulated by antisense rna transcripts. our previous studies have determined that depletion of intracellular polyamines by alpha-difluoromethylornithine results in a marked decrease in the transcription of the human c-myc gene. because of reports that antisense transcription occurs in the 5' and 3' regions of this gene, we used a genomic clone of the human c-myc gene to ascer ... | 1992 | 1378845 |
| insulin-like growth factor ii in two human colon-carcinoma cell lines: gene structure and expression, and protein secretion. | abnormal expression and structural modification of the igf-ii gene in correlation with high igf-ii production have recently been described in human colorectal tumors in comparison with normal adjacent tissues. here, we have studied igf-ii in 2 human colon-carcinoma cell lines, ht29 and colo 320dm. rflp analyses revealed no apparent alteration at the igf-ii locus in these 2 cell lines. ht29 cells weakly expressed igf-ii mrna in comparison with the high over-expression previously observed in some ... | 1992 | 1399116 |
| production of human immunoglobulin g reactive against human cancer in tumor-bearing mice with severe combined immunodeficiency reconstituted with human splenic tissues. | splenic tissues derived from patients with gastric cancer were implanted into mice with severe combined immunodeficiency (scid) and then the mice were challenged with colo-205, a human colon cancer cell line. production of human immunoglobulin g (igg) reactive against the colo-205 cells was detected by enzyme-linked immunosorbent assay in sera from the reconstituted and tumor-bearing scid mice. the titers of the reactive igg relative to total igg in the sera of scid mice began to increase from o ... | 1992 | 1399826 |
| in vitro and in vivo circumvention of multidrug resistance by servier 9788, a novel triazinoaminopiperidine derivative. | s 9788 is a novel triazinoaminopiperidine derivative which does not belong to any of the classes of compounds known to reverse multidrug resistance (mdr). s 9788 was far more potent than verapamil (vrp) in reversing resistance to adriamycin (adr) in the adr-selected murine leukaemia cell lines p388/adr-1 and p388/adr-10, and the human chronic myelogenous leukaemia k562/r. fold reversion with s 9788 (5 microm) was, respectively, 3.5, 5.4 and 11.3 times greater than that with vrp (5 microm). s 978 ... | 1992 | 1428723 |
| stable expression of a cdna encoding a human beta 1 --> 3galactosyltransferase responsible for lacto-series type 1 core chain synthesis in non-expressing cells: variation in the nature of cell surface antigens expressed. | transient expression of a human colonic adenocarcinoma colo 205 cell derived cdna in cell lines which ordinarily express only neolacto-series glycolipids has resulted in the expression of a beta 1 --> 3galactosyltransferase gene responsible for synthesis of glycolipids based upon the lacto-series type 1 core chain. calcium phosphate transfected cells were panned on anti-igm coated plates after initial treatment with a combination of monoclonal antibodies specific for type 1 chain terminal struct ... | 1992 | 1429882 |
| cloning of human tumor cell lines in porous glass capillary tubes: a further development of the human tumor stem cell assay. | the conventional human tumor stem cell assay for cloning tumor cells for drug sensitivity testing is limited by its inability to test drug combinations. in an attempt to overcome this limitation, we cloned tumor cell lines within porous glass capillary tubes. in contrast to plastic porous tubes, the porous glass membranes were transparent, and colony formation could be judged on an inverted microscope. human as well as animal cell lines showed sufficient colony growth. colonies formed within the ... | 1992 | 1460325 |
| [differential diagnostic value of occult blood in stool in hookworm infection in the third world. study from papua new guinea]. | in 633 persons of the madang province in papua-new guinea hookworm-infection, blood hemoglobin and the presence of blood in stools were studied. 60% of the subjects were infected with hookworm (necator americanus). there was a significant linear correlation between the intensity of hookworm infection and blood haemoglobin level. the colo-rectal test gave positive results in 17% of the cases without worm-infection. in infected persons, the colo-rectal test gave an increasing rate of positive resu ... | 1992 | 1471386 |
| antitumor effect of 2'-deoxy-5-fluorouridine conjugates against a murine thymoma and colon carcinoma xenografts. | the conjugation of antineoplastic drugs to monoclonal antibodies reactive with tumor associated antigens conveys selective cytotoxicity, overcoming the systemic toxicities caused by drugs during standard chemotherapy. 2'-deoxy-5-fluorouridine, a more potent derivative of 5-fluorouracil, is an antimetabolite which exerts its cytotoxic action by inhibiting the enzyme thymidylate synthetase and as a result inhibits dna synthesis. 2'-deoxy-5-fluorouridine was successfully conjugated to anti-ly-2.1 r ... | 1992 | 1530766 |
| in vivo selection of a highly metastatic cell line from a human pancreatic carcinoma in the nude mouse. | a cell line with high metastatic capacity to the liver was established by sequential passages of a human pancreatic cancer cell line through the nude mouse liver. a subline, l3.5, established after five passages of the fast-growing variant (fg) of the human pancreatic cancer colo 357 through the nude mouse liver produced extensive hepatic metastases in 100% of experimental animals when injected into the spleen. the incidence of pulmonary metastases decreased from 43% for fg to 9% for l3.5. the l ... | 1992 | 1544114 |
| relationship between the cellular accumulation and the cytotoxicity of s12363, a new vinca alkaloid derivative. | s12363, a new vinca alkaloid derivative, was considerably more cytotoxic to murine l1210 cells and five human tumor cell lines (hl60, ht-29, colo 320dm, nci-h460, and panc-1) than was vincristine (vcr) or vinblastine (vlb). s 12,363 bound to tubulin in crude extracts from brain or l1210 cells with an affinity similar to that of vlb and vcr (apparent kd value: 1.1-1.6, 1.2-1.7, and 0.6-0.8 microm, respectively). after 1 h exposure, the accumulation of 20 nm [3h]-s 12,363 by l1210 cells was 4- to ... | 1992 | 1551175 |
| expression of myc-family genes in established human multiple myeloma cell lines: l-myc but not c-myc gene expression in the u-266 myeloma cell line. | deregulated c-myc expression, as a consequence of translocation of the c-myc gene to one of the immunoglobulin loci, appears to play an important role in the pathogenesis of several b-cell tumors, including burkitt's lymphoma, mouse plasmacytoma and rat immunocytoma. this study investigated the expression of c-myc and 2 other members of the myc gene family, l- and n-myc, at the mrna and protein level, and analyzed for possible rearrangements of these genes in the human counterpart to the mouse p ... | 1992 | 1563831 |
| [supralevator pelvic exenteration with simultaneous bowel and urinary reconstruction. two case reports]. | two male patients underwent supralevator pelvic exenteration, preserving their normal voiding and evacuating function. case 1 was a 19-year-old man with pineal region tumor, and a metastatic lesion in the bottom of the rectovesical pouch, possibly through the ventriculo-peritoneal shunt. following supralevator pelvic exenteration, the construction of double pouches, a colonic j pouch and mainz pouch to the urethra, were performed. case 2 was a 39-year-old man with bulky retrovesical tumor. he un ... | 1992 | 1564840 |
| efficacy of whole-cell pertussis vaccine in preschool children in the united states. | to evaluate the efficacy of currently used whole-cell pertussis vaccines. | 1992 | 1578592 |
| evaluation of four serological tests for bovine paratuberculosis. | the standard complement fixation (cf) test, a commercial agarose gel immunodiffusion (agid) test (immucell corporation, portland, maine), and two commercial enzyme-linked immunosorbent assays (elisas; allied laboratories, glenwood springs, colo. [allied elisa], and the csl, limited, [parkville, victoria, australia] enzyme immunoassay [csl elisa]) were evaluated by using sera from the repository for paratuberculosis specimens. the case definition of subclinical bovine paratuberculosis was isolati ... | 1992 | 1583110 |
| therapeutic effect of the gastrin receptor antagonist, cr2093 on gastrointestinal tumour cell growth. | the gastrin receptor antagonist, cr2093, competed with 125i-gastrin-17 (5 x 10(-10) m) for binding to gastrin receptors on the rat pancreatic adenocarcinoma, ar42j (cr2093 concentration inducing 50% of 125i-gastrin-17 binding (ic50) was 8 x 10(-5) m), on the human gastric adenocarcinoma, mkn45 (ic50 5.5 x 10(-5) m) and the human colo-rectal adenocarcinoma c523 (ic50 greater than 10(-4) m). intravenous administration of cr2093 (40 mg kg-1 day-1) reduced the gastrin-17 stimulated growth of ar42j x ... | 1992 | 1616859 |
| antitumor agent, physalin f from physalis angulata l. | physalin f and physalin d were isolated and characterized from the ethanolic extract of the whole plant of physalis angulata l. (solanaceae). systematic fractionation of the ethanolic extract of the plant led to characterization of physalin f from the fraction paiv-2 as an active ingredient which showed cytotoxicity in vitro by dea and mtt assays on 8 cancer cell lines, five human cancer cell lines: ha22t(hepatoma), hela(cervix uteri), kb(nasopharynx), colo-205(colon) and calu-1(lung); and three ... | 1992 | 1622143 |
| in vitro and in vivo modulation of multi-drug resistance with amiodarone. | the modulating effect on drug resistance of amiodarone (am) and its metabolite desethylamiodarone (dea) was studied in a p-glycoprotein-positive human colon carcinoma cell line colo 320, and a human small-cell lung carcinoma cell line glc4 and its adriamycin (adr)-resistant subline glc4-adr (both p-glycoprotein-negative). am, dea and verapamil induced an increase in cytotoxicity of adr, vincristine and etoposide (vp16) in colo 320 cells, while in the glc4 and glc4-adr cell line no effect was see ... | 1991 | 1646180 |
| p-glycoprotein drug efflux pump involved in the mechanisms of intrinsic drug resistance in various colon cancer cell lines. evidence for a saturation of active daunorubicin transport. | we studied the resistance of colon tumors to anticancer agents in vitro. using daunorubicin (dn), a number of cellular parameters which normally indicate acquired or multidrug resistance (mdr), were compared for several human wild-type colon cell lines, i.e. ht29, sw1116 and colo 320, and the murine colon cell line c-26. the sensitive/mdr human ovarian cancer cell line couple a2780/2780ad was used as a reference. the amount of p-glycoprotein (p-gp) was in the order ht29, a2780 less than or equal ... | 1991 | 1671638 |
| absorption and metabolism of purines by the lower intestine of the chicken. | 1. absorption of purines and their metabolism by the lower intestine were estimated by using the everted gut sacs from the colo-rectum and caecum of the chicken. 2. adenine, hypoxanthine and uric acid were appreciably absorbed from the colo-rectum and caecum, and an especially high rate was observed in the absorption of uric acid from the colo-rectum. 3. guanine was not absorbed unchanged from either the colo-rectum or the caecum and a small amount of xanthine was absorbed only from the caecum. ... | 1991 | 1682101 |
| frequency and spectrum of mutations at codons 12 and 13 of the c-k-ras gene in human tumors. | the frequency of point mutations at codons 12 and 13 of the c-k-ras gene has been determined in a panel of more than 400 human tumors. mutant c-k-ras genes were detected in about 75% of adenocarcinomas of the pancreas (n = 84); 40% of adenomas (n = 72) and carcinomas (n = 244) of the colon end rectum; 30% of carcinomas of the bile duct (n = 19); 25% of carcinomas of the lung (n = 92), and in lower frequency in other carcinomas, including liver, stomach, and kidney. no mutations were found in car ... | 1991 | 1685441 |
| fibronectin, laminin, and collagen types i, iii, iv and v in the healing rat colon anastomosis. | the purpose of this work was to study normal anastomotic healing in the rat colon. the unprepared sigmoid colon was divided and a colo-colostomy performed using a one-layer inverting technique. frozen sections were taken and studied immunohistologically with specific antibodies to fibronectin, laminin and collagen types i, iii, iv and v. from day one onwards a strong fibronectin reaction was observed in the anastomosis, reaching maximum staining intensity on postoperative day five. type iii coll ... | 1990 | 1696798 |
| identification and characterization of two distinct alpha-(1-3)-l-fucosyltransferase activities in human colon carcinoma. | two distinct alpha-(1-3)-fucosyltransferase activities have been identified in the colon carcinoma cell lines ht-29 and colo-205. while both enzymatic activities exhibit similar affinities for a synthetic alpha-(1-3) acceptor and gdp-fucose, they differ with respect to divalent cation requirements, n-ethylmaleimide inhibition, and glycoprotein substrate specificity. the colo-205 alpha-(1-3) activity exhibits maximal enzymatic activity in the presence of 20 mm mn2+ but retains less than 10% activ ... | 1990 | 1698542 |
| [colo-rectal spirochetosis: is it an anatomo-pathologic entity?]. | nineteen colorectal biopsy specimens, stained by hematein-eosin-safran (hes), were examined by light microscopy and showed a thick, blue and fuzzy brush border. without any further microbiologic investigation, this histologic feature is considered strongly suggestive of colorectal spirochetosis. our study concerned 19 male patients aged between 35 and 68 years, who had no risk factor for hiv infection, but who belonged to these three groups: (a) those suffering from chronic diarrhea; (b) those w ... | 1990 | 1701640 |
| inhibition of c-myc expression in human promyelocytic leukemia and colon adenocarcinoma cells by 6-thioguanine. | a rapid decrease in expression of the oncogene c-myc has been associated with the induction of differentiation of hl-60 human leukemia cells. in this manner, the treatment of a hypoxanthine phosphoribosyltransferase (hprt)-deficient hl-60 variant (hl-60/var) with 6-thioguanine (tg) was accompanied by lower c-myc mrna levels. this occurred in the absence of 6-thioguanosine 5'-monophosphate (tgmp) synthesis and without alterations in cellular nucleotide pool sizes. paradoxically, inhibition of c-m ... | 1990 | 1702632 |
| isolation and characterization of a human monoclonal antibody which reacts with breast and colorectal carcinoma. | a human monoclonal antibody with specificity for breast and colorectal carcinoma has been isolated after hybridoma formation between regional lymph node lymphocytes from a patient with breast carcinoma and a mouse x human heteromyeloma (spaz-4). the monoclonal, mab 15.2.3, is an igm, kappa, which binds to an epitope found on at least four glycoproteins with approximate molecular weights of 37, 39, 43 and 56 kilodaltons (kd). these antigens are expressed intracellularly and on the surface of brea ... | 1990 | 1704693 |
| characterization of a novel monoclonal antibody, 3h-1, reactive with squamoproliferative lesions and squamous-cell cancers. | immunization of mice with membranes from a virus-transformed human keratinocyte cell line (kjd-i/sv40) yielded an igm monoclonal antibody (mab 3h-i) which reacted with the membrane and cytoplasm of kjd-i/sv40 cells and in the perinuclear region of a squamous-cell carcinoma line (colo-16). immunohistochemistry of formalin-fixed, paraffin-embedded sections using mab 3h-i gave intense staining of proliferating squamous epithelium in several characteristic patterns. acanthotic squamous epithelium an ... | 1991 | 1707035 |
| tumour-growth suppression in nude mice by a murine monoclonal antibody: factors hampering successful therapy. | the murine mab c215 has been shown to mediate admmc in vitro and to have a tumour-growth-suppressive effect on xenografted colo 205 human colocarcinoma cells in nude mice. to overcome the limitations of mab therapy, it is necessary to understand the underlying mechanisms of tumour-growth suppression. in the present work, we have used c215 to define the importance of different parameters involved in tumour therapy with murine igg2a antibodies. the results show that there exists a period of roughl ... | 1991 | 1708368 |
| production of muc1 and muc2 mucins by human tumor cell lines. | a mucus secreting, clonal derivative (ht29-sb) of the human colonic adenocarcinoma cell line ht29, and the ls174t colon cancer cell line, secrete mucin into the culture medium as a viscoelastic gel. mab bc2, which defines a peptide epitope present in the variable number of tandem repeats (vntr) of the muc1 core protein, reacted with this material after deglycosylation. two high molecular weight bands were detected in tfmsa treated gel-formed mucin from ht29-sb and ls174t by western blotting (mr ... | 1991 | 1713449 |
| molecular cloning of the human histamine h2 receptor. | we utilized the technique of polymerase chain reaction with oligonucleotide primers based upon the nucleotide sequence of the canine h2 histamine receptor gene which we recently isolated to clone its human homologue. transfection of a construct of this gene in colo-320 dm cells led to the expression of a receptor that bound to [methyl-3h] tiotidine and was linked to 3',5'cyclic adenosine monophosphate (camp) generation in response to histamine. both camp generation and [methyl-3h] tiotidine bind ... | 1991 | 1714721 |
| purification and characterization of a membrane-bound and a secreted mucin-type glycoprotein carrying the carcinoma-associated sialyl-lea epitope on distinct core proteins. | two mucin-type glycoproteins detected by the monoclonal antibody c50, which reacts with the carcinoma-associated sialyl-lewis a and sialyl-lactotetraose epitopes, were found in secreted and solubilized materials from the colon carcinoma cell line colo 205. the larger glycoprotein (h-canag; heavy cancer antigen) was predominantly found in extracts of cells grown in vitro or as nude mice xenografts whereas the smaller species (l-canag; light cancer antigen) was the major component in spent culture ... | 1991 | 1718981 |
| biosynthesis in vitro of sa-lex and sa-dilex by alpha 1-3 fucosyltransferases from colon carcinoma cells and embryonic brain tissues. | the sialyl-fucosyl-lactosamine-epitope present in sialyl (sa)-lex (neuac alpha 2-3gal beta 1-4 [fuc alpha 1-3]glcnac beta 1-3gal beta 1-4glc-cer), a carcinoembryonic antigen, has been recognized recently as a ligand for the binding of leukocyte-endothelial cell adhesion molecule 1 (lecam-1) to myeloid and tumour cell surfaces. we have recently detected the presence of an alpha 1-3 fucosyltransferase (fuct-3) activity in both embryonic chicken brain (ecb) and human colon carcinoma cells (colo-205 ... | 1991 | 1726578 |
| enhanced cmp-neuac:gal beta 1,4glcnac-r alpha 2,6 sialyltransferase activity of human colon cancer xenografts in athymic nude mice and of xenograft-derived cell lines. | the activity of an alpha 2,6 sialyltransferase acting on n-acetyllactosaminic sequences (alpha 2,6 st e.c. 2.4.99.1) has previously been found to be increased in 90% of human colon cancer specimens. in the present study, the alpha 2,6 st activity of 6 human colon cancer cell lines grown in culture was compared with that expressed by the corresponding nude mice xenografts and by the cell lines derived from the xenografts. we found that xenografts of colo 205, ht-29, sw 620, sw 948 and sw 948 fl ( ... | 1992 | 1730528 |
| human non-hepatocytes support hepadnaviral replication and virion production. | the competence of non-hepatocytes to support hepatitis b virus (hbv) gene expression and replication was studied by transient transfection of various human cell lines with a head-to-tail dimer of hbv dna. independent of their neuroectodermal, mesenchymal or epithelial origin, all non-hepatocyte cell lines tested synthesized and secreted hepatitis b surface antigen (hbsag) and hepatitis b core/e antigen (hbc/eag). further analyses of two of these cell lines (ls 180 and colo 320) identified the tw ... | 1992 | 1730939 |
| growth-inhibitory effect of quercetin and presence of type-ii estrogen-binding sites in human colon-cancer cell lines and primary colorectal tumors. | we studied the effect of quercetin (q) on the proliferation of ht-29, widr, colo 201, and ls-174t human colon cancer cell lines. q, between 10 nm and 10 microm, exerted a dose-dependent, reversible inhibition of cell proliferation. cell-cycle analysis revealed that the growth-inhibitory effect of q was due to a blocking action in the g0/g1 phase. using a whole-cell assay with 17 beta-[3h]-estradiol as tracer, we demonstrated that all these cell lines contain type-ii estrogen-binding sites (type- ... | 1992 | 1735617 |
| a novel beta 4, alpha 6 integrin-associated epithelial cell antigen involved in natural killer cell and antigen-specific cytotoxic t lymphocyte cytotoxicity. | efficient immune responses require interactions between cell adhesion molecules on lymphocytes and counter-receptors on antigen presenting cells or target cells. while target-specific receptors or ligands have not been identified for natural killer (nk) cells, cell adhesion molecules have been implicated in the interaction between nk cell effectors and tumor cell targets. herein, we describe monoclonal antibodies (mabs) against a carcinoma cell line that efficiently block the cytolytic activity ... | 1991 | 1744585 |
| heterogeneity in antigenic expression and radiosensitivity in human colon carcinoma cell lines. | a panel of human colon carcinoma cell lines were characterized regarding both antigenic heterogeneity and variations in radiosensitivity. monoclonal antibodies were used to study the expression of carcinoembryonic antigen (cea), gastrointestinal cancer antigen (gica or ca 19-9) and carcinoma-associated antigen (ca-50). radiosensitivity was studied with the clonogenic survival technique. three cell lines, ls 174t, hctc, and sw 1116 stained positive for all three antigens. ht-29 was positive for c ... | 1991 | 1757394 |
| cytotoxic activities of tanshinones against human carcinoma cell lines. | fifteen tanshinone analogues isolated from the chloroform extract of danshen roots (salviae miltiorrhizae radix) by chromatographic procedures were tested for their cytotoxic activities against kb, hela, colo-205 and hep-2 carcinoma cell lines. several of them were effective at concentrations below 1 micrograms/ml concentrations. tanshinone analogues with either hydroxy substitutions or olefinic feature in ring a demonstrated higher biologic activities. analysis of structure-activity relationshi ... | 1991 | 1767792 |
| endoluminal prosthesis in protecting high-risk colonic anastomosis: an experimental study. | an experimental study was carried out to evaluate the protective role of an endoluminal prosthesis on high risk colo-colonic anastomoses. one hundred and fifty rats were divided into two groups: group e with endoluminal latex prosthesis and group c without. bursting pressure and collagen concentration in the perianastomotic tissue were tested, respectively, on the 4th, 7th and 12th, and 4th, 7th, 12th, 18th and 38th postoperative days. bursting pressure was significantly higher in group e on the ... | 1991 | 1770286 |
| loss of amplified genes by poly(adp-ribose) polymerase inhibitors. | a poly(adp-ribose) polymerase inhibitor, benzamide (ba), was found to induce flat revertants of nih 3t3 cells that had been transformed by human ha-ras, rat ki-ras, rat c-raf, and human ret-ii. these genes had been amplified in original transformants, but they were completely eliminated by ba. contrary to this, endogenous activated ha-ras in a human bladder carcinoma cell line, t24, was not eliminated by ba. the gene loss seemed to be restricted to exogenous and/or amplified sequences. ba also e ... | 1991 | 1773788 |
| [nitrogen composition of the fruit juice of the persian lime in cuba]. | in 1987 to 1989 the composition was studied of the nitrogen compounds (nitrogen and free amino acids) in the fruit juice of persian lime during its commercial harvesting period. adult persian lime trees grafted on citrus macrophylla and c. volkameriana were used, planted on a groundwater-affected red ferrilytic soil in the la habana province. determination of the total nitrogen and of the free proline was carried out in may to july of each experimental year. the amino acid spectrum was establish ... | 1991 | 1823779 |
| biosynthesis in vitro of core lacto-series glycosphingolipids by n-acetyl-d-glucosaminyltransferases from human colon carcinoma cells, colo 205. | two n-acetyl-d-glucosaminyltransferases have been detected in human colon carcinoma colo 205 cells. these enzymes catalyze the biosynthesis in vitro of the core-glycolipid of type 1 and type 2 lacto-series antigens and of the polylactosamine-containing longer chain antigenic structures, respectively. the first enzyme, glcnact-1, which catalyzes the formation of lactotriosylceramide [lcose3cer, beta-d-glcpnac-(1----3)-lcose2cer, the core for all lacto-series type 1 and type 2 chains] from lactosy ... | 1991 | 1828006 |
| the effects of gamma-interferon combined with 5-fluorouracil or 5-fluoro-2'-deoxyuridine on proliferation and antigen expression in a panel of human colorectal cancer cell lines. | gamma-interferon (ifn-gamma) and the antimetabolites 5-fluorouracil (5-fu) and 5-fluoro-2'-deoxyuridine (fudr) were investigated as individual agents and in combination for their in vitro antiproliferative capacity and for their effect on the expression of hla class-i antigen, carcinoembryonic antigen (cea) and the intracellular tumor-associated antigen cta-i in 7 human colorectal cancer cell lines: widr, ht29, colo 205, sw116, ls174t, sw1398, and lovo. growth inhibition by ifn-gamma at clinical ... | 1991 | 1830034 |
| [ultrasonic diagnosis of colonic invagination]. | intussusception is a rare condition in adults. in the large intestine, intussusception is most frequently caused by malignant tumours. the case history of a man aged 35 years is presented here. in connection with operation for colo-colic intussusception, familial polyposis was demonstrated. the diagnosis of colo-colic intussusception was established by ultrasonic scanning. | 1991 | 1846484 |
| [fecal occult blood--does a hemoglobin-specific tests improve the diagnosis of relevant colonic neoplasms?]. | the object of this study was to assess whether an immunological test specific for human hemoglobin improves the detection of bleeding from colorectal tumors as compared to a conventional guaiac test for occult fecal blood. we examined one stool sample from 134 patients prior to a complete colonoscopy by the colo-immun-test (ci) as well as the colo-rectal-test (cr). ci proved to have a higher sensitivity for occult bleeding from all colonic lesions, but this difference was not statistically signi ... | 1991 | 1862316 |
| [pharmacokinetics and tissue penetration of single-dose netilmicin used for antibiotic prophylaxis during colo-rectal surgery]. | pharmacokinetics and tissue penetration of netilmicin were studied after the use of a single dose (6 mg/kg) given for antibioprophylaxis in colo-rectal surgery. thirteen patients, scheduled for elective surgery, were given 6 mg/kg iv netilmicin over 30 min, together with 1000 mg iv ornidazole. netilmicin peak serum concentration (10 min after end of infusion) was 24.4 +/- 3.4 mg/l and trough level (24 h) was 0.9 +/- 0.5 mg/l. plasma elimination half-life was 409 +/- 70 min, le volume apparent vo ... | 1991 | 1881684 |
| oxidative polymorphism of debrisoquine is not related to human colo-rectal cancer. | the oxidative polymorphism of debrisoquine (dbq) has been determined in 89 patients with colo-rectal cancer and in 556 normal control subjects. four patients and 34 controls, with a metabolic ratio greater than 12.6, were classified as poor metabolisers of dbq (n.s.). no difference was found in the distribution of the frequencies of the mr of dbq between patients and controls. it is concluded that polymorphic oxidation of dbq is not related to the risk of developing colo-rectal cancer in human b ... | 1991 | 1884729 |
| an evaluation of ovarian carcinoma-associated antigen defined by murine monoclonal antibody cf511 in sera from patients with ovarian carcinoma. | murine monoclonal antibody cf511, raised against human ovarian clear cell carcinoma, detects a glycoprotein (mr 600 kda) called cf511 antigen which is elevated in the serum of many patients with ovarian carcinoma. a competitive enzyme-linked immunosorbent assay was developed to detect cf511 antigen in human serum and used to detected cf511 antigen in subjects with ovarian carcinoma and other diseases. no raised levels (less than 18 unit (u) ml-1) of the antigen were found in the serum of 220 nor ... | 1991 | 1892754 |
| alpha 2,6 sialylation of n-acetyllactosaminic sequences in human colorectal cancer cell lines. relationship with non-adherent growth. | in a previous work we found that human colorectal cancer tissues express increased levels of an alpha 2,6 sialyltransferase (alpha 2,6 st) acting on n-acetyllactosaminic sequences (e.c. 2.4.99.1). in this study we have taken advantage of the known specificity of elderberry bark lectin (sambucus nigra agglutinin, sna) for neuac alpha 2, 6gal/galnac structures to investigate the relationship between expression of alpha 2,6 sialyltransferase activity and occurrence of alpha 2,6-sialylated oligosacc ... | 1991 | 1899084 |
| kupffer cell-mediated oxidative stress on colon cancer cell line visualized by digital imaging fluorescence microscopy. | temporal and spatial changes of lipid peroxides in a cultured colon cancer cell line, colo-205 cells, were investigated after culturing with kupffer cells by using 2',7'-dichlorofluorescein diacetate and a digital imaging processor equipped with an inverted microscope. by this method, we successfully visualized the alteration of lipid peroxides in the individual cancer cell. without any prior activation, kupffer cells isolated from an intact rat liver caused rapid increase in the intensity of di ... | 1991 | 1913616 |
| differential expression of two classes of lck transcripts upon phorbol ester treatment of human leukemic t cells. | the human lymphocyte-specific tyrosine kinase gene, lck, is transcribed from two distinct promoters, resulting in two classes of transcripts (type i and ii) differing in their 5' untranslated regions. the steady-state levels of the type i and ii lck transcripts were measured in a variety of lymphoid and non-lymphoid human tumor cell lines by s1 nuclease mapping and by a sensitive assay system using the polymerase chain reaction. human thymocytes and all the leukemic t cell lines tested express b ... | 1991 | 1918168 |
| photocytotoxic efficacy of sulphonated species of aluminium phthalocyanine against cell monolayers, multicellular spheroids and in vivo tumours. | the problem of relying solely on in vitro data to predict photosensitiser efficacy was demonstrated by examining the uptake and the ability to mediate photocytotoxicity of mono-, di-, tri- and tetra-sulphonated species of chloroaluminium phthalocyanine (als1-4pc) in monolayer cultures of murine colo 26 cells and in both monolayer and spheroid cultures of human widr cells. cells treated in vitro, whether in monolayer or as spheroids, with the less sulphonated derivatives, als1pc and als2pc, were ... | 1991 | 1931602 |
| differential induction of secondary dna fragmentation by topoisomerase ii inhibitors in human tumor cell lines with amplified c-myc expression. | in order to understand the cellular events associated with cell death after the formation of topoisomerase ii-dna cleavable complexes, we compared the induction of endonucleolytic dna fragmentation by etoposide and its more potent analog, teniposide (vm-26) in the human cell lines ht-29 and hl-60. a new filter-binding assay is described, which allows rapid quantification of nonprotein-linked dna fragmentation involved in apoptosis. both cell lines showed similar loss of colony formation ability ... | 1991 | 1933888 |
| chromosome analysis on two long-term established human colorectal carcinoma cell lines. | a chromosomal analysis was carried out on two colorectal carcinoma cell lines (widr and colo 205), which were established 15-20 years ago in the us and were collected by the cell bank of the veterans general hospital in taipei. among the 200 cells counted, 65.5% of widr (male) had 68-73 chromosomes, while 74% of the colo 205 (female) had 70-76 chromosomes per cell. the y chromosome was absent in the 30 widr metaphases analyzed. none of the other chromosomes, including x and the autosomes of both ... | 1991 | 1946816 |
| udp-galnac:neuac alpha 2,3gal beta-r (galnac to gal) beta 1,4-n-acetylgalactosaminyltransferase responsible for the sda specificity in human colon carcinoma caco-2 cell line. | the udp-galnac:neuac alpha 2,3gal beta-r (galnac to gal) beta-1,4-n-acetylgalactosaminyltransferase (beta 1,4galnac-transferase) is the enzyme responsible for the addition of the immunodominant sugar of the sda isto-blood group determinant. in humans the enzyme is mainly expressed in the large intestine. we screened nine human colorectal carcinoma cell lines (sw-948, sw-948 fl, sw-480, sw-48, sw-1417, colo-205, lovo, ht-29 and caco-2) in order to ascertain the occurrence of beta 1,4galnac-transf ... | 1991 | 1953740 |
| differential expression of intercellular adhesion molecule 1 in primary and metastatic melanoma lesions. | immunochemical studies have shown that the monoclonal antibody (moab) cl203.4, elicited with immune interferon treated cultured human melanoma cells colo 38, recognizes intercellular adhesion molecule 1 (icam-1). the determinant defined by moab cl203.4 is distinct and spatially distant from that defined by anti-icam-1 moab rr1/1, which had been elicited with epstein-barr virus-transformed b-lymphocytes from a lymphocyte function associated antigen 1 deficient patient. immunohistochemical testing ... | 1990 | 1967552 |
| human colon carcinoma, fibrosarcoma and leukemia cell lines produce tumor-associated trypsinogen. | previous studies have indicated that cyst fluid of ovarian tumors contains 2 trypsinogen isoenzymes, called tumor-associated trypsinogen-i (tat-i) and trypsinogen-2 (tat-2), the levels of which correlate with the degree of malignancy of the tumors. in addition, these cyst fluids contain large amounts of tumor-associated trypsin inhibitor (tati), which is also expressed in many other human tumors. in the present study we examined the production of tat-i, tat-2 and tati in 9 established tumor-cell ... | 1991 | 1995487 |
| inhibition of proliferation by c-myb antisense oligodeoxynucleotides in colon adenocarcinoma cell lines that express c-myb. | steady-state mrna levels of the protooncogene c-myb were measured by northern blot analysis in the human colon carcinoma cell lines lovo, the doxorubicin-resistant derivative lovo/dx, colo 205, and ht 29. overexpression of c-myb mrna was detected in the colo 205 cell line, probably because of gene amplification, while in human ht 29 cells c-myb was not expressed at a detectable level. comparison between lovo and lovo/dx cell lines showed that c-myb mrna levels were much higher in the doxorubicin ... | 1991 | 2032228 |
| elevated expression of phosphatidylserine in the outer membrane leaflet of human tumor cells and recognition by activated human blood monocytes. | we determined whether the presence of phosphatidylserine (ps) in the outer membrane leaflet of human tumor cells correlated with their recognition by activated human monocytes. three tumorigenic cell lines, a375 melanoma and a431 and colo-16 carcinomas, and a normal human epidermal keratinocyte line (nhek) were incubated with monocytes activated to the tumoricidal state by gamma-interferon and lipopolysaccharide. activated human monocytes bound to and lysed all tumorigenic targets, while the non ... | 1991 | 2032247 |
| microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro. | the growth kinetics of established human colorectal tumour cell lines (ht29, ht115 and colo 320dm) and human diploid fibroblasts (flow 2002) were studied in conventional culture and in microcapsules formed from alginate-poly(l-lysine)-alginate membranes. the tumour lines grew rapidly in microcapsules but, in the case of the substrate-adherent lines ht29 and ht115, only after a prolonged lag phase. this phase was reduced by serial passage in microcapsules. the anchorage-independent line colo 320d ... | 1991 | 2039691 |