| effect of light energy on alkali-released virions from anagrapha falcifera nucleopolyhedrovirus. | we compared the insecticidal activities of occluded and nonoccluded afmnpv baculovirus obtained by dissolving the occlusion bodies (ob) with sodium carbonate. droplet feeding and cotton leaf feeding bioassay techniques were used to determine the dose response against neonate trichoplusia ni (hübner) and loss of insecticidal activity when the virus was exposed to simulated sunlight from a xenon light source. using droplet bioassays to determine a dose response, nonoccluded virus (nov) was 20 time ... | 2000 | 11023735 |
| effect of transgenic plants expressing high levels of a tobacco anionic peroxidase on the toxicity of anagrapha falcifera nucleopolyhedrovirus to helicoverpa zea (lepidoptera: noctuidae). | wild type and corresponding transgenic tomato (lycopersicon esculentum miller) and two tobacco (nicotiana spp.) plants that express high levels of a tobacco anionic peroxidase were used to determine what type of interactions occurred between peroxidase altered plant chemistry and the baculovirus anagrapha falcifera nucleopolyhedrovirus (afmnpv) for control of neonate corn earworms, helicoverpa zea (boddie). transgenic plants expressed approximately five to 400 times higher peroxidase activity th ... | 2002 | 11942768 |
| the nucleopolyhedroviruses of rachiplusia ou and anagrapha falcifera are isolates of the same virus. | the 7.8 kb ecori-g fragment of rachiplusia ou multicapsid nucleopolyhedrovirus (romnpv), containing the polyhedrin gene, was cloned and sequenced. the sequence of the fragment was 92.3% identical to the sequence of the corresponding region in the autographa californica (ac)mnpv genome. a comparison of the ecori-g sequence with other mnpv sequences revealed that romnpv was most closely related to acmnpv. however, the predicted amino acid sequence of romnpv polyhedrin shared more sequence identity ... | 1999 | 10573177 |
| detection and identification of multiple baculoviruses using the polymerase chain reaction (pcr) and restriction endonuclease analysis. | a technique using the polymerase chain reaction (pcr) and restriction analysis was developed for the simultaneous detection of eight baculoviruses. the baculoviruses detected by this technique were autographa californica multiple-embedded nuclear polyhedrosis virus (mnpv). anticarsia gemmatalis mnpv, bombyx mori mnpv, orgyia pseudotsugata mnpv. spodoptera frugiperda mnpv, s. exigua mnpv, anagrapha falcifera mnpv, heliothis zea single-embedded nuclear polyhedrosis virus (snpv). a highly conserved ... | 1997 | 9015292 |
| storage stability of anagrapha falcifera nucleopolyhedrovirus in spray-dried formulations. | a multiply embedded nucleopolyhedrovirus isolated from anagrapha falcifera (kirby) (afmnpv) can lose insecticidal activity during months of dry storage in ambient room conditions. we tested the spray-dried afmnpv formulations after storage for up to 1 year at room temperatures for insecticidal activity against neonate trichoplusia ni (hübner). experimental formulations were made using combinations of corn flours, lignin, and sucrose, and were selected based on previous work which demonstrated th ... | 2002 | 12054782 |
| field activity and storage stability of anagrapha falcifera nucleopolyhedrovirus (afmnpv) in spray-dried lignin-based formulations. | a multiple-embedded nucleopolyhedrovirus isolated from anagrapha falcifera (kirby) (afmnpv) has potential to be developed into a microbial bioinsecticide because the host range includes several economic pests. we tested spray-dried afmnpv formulations after storage for insecticidal activity based on bioassays with neonate trichoplusia ni (hübner). eight experimental lignin-based spray-dried formulations, a glycerin-based formulation, and an unformulated sample were made with virus stock from thr ... | 2003 | 14503576 |