| identification and characterisation of the antimicrobial peptide, phylloseptin-pt, from the skin secretion of phyllomedusa tarsius, and comparison of activity with designed, cationicity-enhanced analogues and diastereomers. | antimicrobial peptides belonging to the phylloseptin family are mainly found in phyllomedusine frogs. these peptides not only possess potent antimicrobial activity but exhibit low toxicity against eukaryotic cells. therefore, they are considered as promising drug candidates for a number of diseases. in a recent study, potent antimicrobial activity was correlated with the conserved structures and cationic amphiphilic characteristics of members of this peptide family. a phylloseptin peptide precur ... | 2016 | 27918477 |
| discovery of phylloseptins that defense against gram-positive bacteria and inhibit the proliferation of the non-small cell lung cancer cell line, from the skin secretions of phyllomedusa frogs. | the growing occurrence of bacterial resistance to conventional antibiotics has called for the development of new classes of antimicrobial agents. antimicrobial peptides (amps) with broad antimicrobial spectrum derived from frog skin secretions have been demonstrated to be promising candidates for new antibiotic development. a proven rich source of these compounds are the skin secretions of the frogs in the phyllomedusa genus. in this study, two novel phylloseptin peptides-phylloseptin-pta and ph ... | 2017 | 28850103 |
| targeted modification of a novel amphibian antimicrobial peptide from phyllomedusa tarsius to enhance its activity against mrsa and microbial biofilm. | antimicrobial peptides (amps) in the skin secretions of amphibians are fundamental components of a unique defense system that has evolved to protect these hosts from microbial invasion. medusins constitute a recently-discovered amp family from phyllomedusine leaf frog skin and exhibit highly-conserved structural characteristics. here, we report a novel medusin, medusin-pt, from the skin secretion of the tarsier leaf frog, phyllomedusa tarsius. the mature peptide was initially identified from its ... | 2017 | 28469603 |
| structure and function of a novel antioxidant peptide from the skin of tropical frogs. | the amphibian skin plays an important role protecting the organism from external harmful factors such as microorganisms or uv radiation. based on biorational strategies, many studies have investigated the cutaneous secretion of anurans as a source of bioactive molecules. by a peptidomic approach, a novel antioxidant peptide (aop) with in vitro free radical scavenging ability was isolated from physalaemus nattereri. the aop, named antioxidin-i, has a molecular weight [m+h]+ = 1543.69da and a twyf ... | 2018 | 29162516 |
| a new morphologically cryptic species of phyllomedusa (anura: phyllomedusidae) from amazonian forests of northern peru revealed by dna sequences. | we describe and name phyllomedusa chaparroi sp. nov., a medium-sized species (snout-vent length in adult males 67.9-77.5 mm) of monkey frog from amazonian rainforests of northern peru. although morphologically most similar to p. boliviana and p. camba (indistinguishable from the latter in external qualitative and quantitative traits), phylogenetic analysis of combined mitochondrial and nuclear markers place the new species sister to a clade containing p. neildi, p. tarsius, and p. trinitatis. ph ... | 2017 | 28610333 |
| a novel dermaseptin isolated from the skin secretion of phyllomedusa tarsius and its cationicity-enhanced analogue exhibiting effective antimicrobial and anti-proliferative activities. | a novel dermaseptin peptide, dermaseptin-pt9 (dpt9), was isolated and identified from phyllomedusa tarsius by the combination of molecular cloning and lc-ms analysis. chemically synthesised dpt9 was broadly effective against the tested microorganisms through the disruption of cell membranes and showed weak haemolytic activity towards horse erythrocytes. it also exhibited anti-proliferative effect against various human cancer cells. moreover, an analogue with enhanced cationicity, k8, 23-dpt9, in ... | 2019 | 31635388 |
| peptidomic analysis of the host-defense peptides in skin secretions of the trinidadian leaf frog phyllomedusa trinitatis (phyllomedusidae). | peptidomic analysis (reversed-phase hplc combined with maldi-tof mass spectrometry and automated edman degradation) of norepinephrine-stimulated skin secretions from the trinidadian leaf frog phyllomedusa trinitatis mertens 1926 led to the identification and structural characterization of 26 host-defense peptides. on the basis of amino acid sequence similarity, the peptides may be divided into the followings groups: dermaseptins with the conserved n-terminal region glwskik (6 peptides), dermasep ... | 2018 | 29980138 |
| structure and membrane interactions of the homodimeric antibiotic peptide homotarsinin. | antimicrobial peptides (amps) from amphibian skin are valuable template structures to find new treatments against bacterial infections. this work describes for the first time the structure and membrane interactions of a homodimeric amp. homotarsinin, which was found in phyllomedusa tarsius anurans, consists of two identical cystine-linked polypeptide chains each of 24 amino acid residues. the high-resolution structures of the monomeric and dimeric peptides were determined in aqueous buffers. the ... | 2017 | 28102305 |