detection and characterization of two chimpanzee polyomavirus genotypes from different subspecies. | the complete nucleotide sequences of three chimpanzee polyomavirus genetic variants were determined. phylogenetic analysis indicated that the viruses form two different genotypes of chpyv. comparison with other primate polyomaviruses revealed a putative agnogene, and an unusually long vp1 open reading frame. the transcriptional control regions (tcr) of the viruses were extremely short (155 nucleotides), and highly conserved amongst the genotypes. analysis of the tcr from different chimpanzee sub ... | 2010 | 21110837 |
production and biomedical applications of virus-like particles derived from polyomaviruses. | virus-like particles (vlps), aggregates of capsid proteins devoid of viral genetic material, show great promise in the fields of vaccine development and gene therapy. these particles spontaneously self-assemble after heterologous expression of viral structural proteins. this review will focus on the use of virus-like particles derived from polyomavirus capsid proteins. since their first recombinant production 27 years ago these particles have been investigated for a myriad of biomedical applicat ... | 2013 | 23999392 |
novel polyomavirus detected in the feces of a chimpanzee by nested broad-spectrum pcr. | in order to screen for new polyomaviruses in samples derived from various animal species, degenerated pcr primer pairs were constructed. by using a nested pcr protocol, the sensitive detection of nine different polyomavirus genomes was demonstrated. the screening of field samples revealed the presence of a new polyomavirus, tentatively designated chimpanzee polyomavirus (chpyv), in the feces of a juvenile chimpanzee (pan troglodytes). analysis of the region encoding the major capsid protein vp1 ... | 2005 | 15731285 |
complete genome sequence of a novel chimpanzee polyomavirus from a western common chimpanzee. | we report here the full-length genome sequence of a novel chimpanzee polyomavirus. viral sequences were recovered from colon, bladder, and ureter tissue from a western common chimpanzee. the virus is genetically closely related to the human bk polyomavirus. | 2016 | 26798086 |
detection of chimpanzee polyomavirus-specific antibodies in captive and wild-caught chimpanzees using yeast-expressed virus-like particles. | chimpanzee polyomavirus (chpyv) was originally detected in the faeces of a captive chimpanzee by random screening using broad-spectrum pcr. its pathogenicity and the distribution among chimpanzees are unknown so far. here, the major capsid protein vp1 of chpyv was expressed in yeast cells. virus-like particles (vlps) with a diameter of approximately 45nm were demonstrated although the efficiency of vlp formation was low as compared to monkey polyomavirus sv40-vlps. the chpyv-vlp preparation did ... | 2010 | 21187117 |