| rnai-mediated transgenic tospovirus resistance broken by intraspecies silencing suppressor protein complementation. | extension of an inverted repeat transgene cassette, containing partial nucleoprotein (n) gene sequences from four different tomato-infecting tospovirus spp. with a partial n gene sequence from the tomato strain of tomato yellow ring virus (tyrv-t), renders transgenic nicotiana benthamiana plants additionally resistant to this strain but not to the soybean strain of this tospovirus sp. (tyrv-s), both strains exhibiting 14.4% nucleotide sequence divergence in their n genes. surprisingly, coinocula ... | 2009 | 19737098 |
| diverging affinity of tospovirus rna silencing suppressor proteins, nss, for various rna duplex molecules. | the tospovirus nss protein was previously shown to suppress the antiviral rna silencing mechanism in plants. here the biochemical analysis of nss proteins from different tospoviruses, using purified nss or nss containing cell extracts, is described. the results showed that all tospoviral nss proteins analyzed exhibited affinity to small double-stranded rna molecules, i.e., small interfering rnas (sirnas) and micro-rna (mirna)/mirna* duplexes. interestingly, the nss proteins from tomato spotted w ... | 2010 | 20739523 |
| a member of a new tospovirus species isolated in italy from wild buckwheat (polygonum convolvulus). | electron microscopy of extracts from diseased polygonum convolvulus plants from piedmont (italy) revealed particles with the morphological features of a tospovirus. sequencing of the full-length small (s) and medium (m) genome segments indicated that the virus is a member of a new tospovirus species provisionally named polygonum ringspot virus. a feature distinguishing it from members of other tospovirus species was the presence of a very short intergenic region on the s segment lacking the pote ... | 2008 | 18953484 |
| a new tomato-infecting tospovirus from iran. | abstract a new tospovirus species serologically distinct from all other established tospoviruses was found in tomato in iran. typical disease symptoms observed include necrotic lesions on the leaves and yellow ring spots on the fruits, hence the name tomato yellow ring virus (tyrv) was proposed. the s rna of this virus was cloned and its 3,061 nucleotide long sequence showed features characteristic for tospoviral s rna segments. the nucleocapsid (n) protein with a predicted mr of 30.0 kda showed ... | 2005 | 18944405 |
| molecular and biological comparison of two tomato yellow ring virus (tyrv) isolates: challenging the tospovirus species concept. | two strains of tomato yellow ring virus (tyrv, genus tospovirus), one from tomato (referred to as tyrv-t) and the other from soybean and potato (denoted tyrv-s), collected from different geographical regions in iran, were compared. their genomic s rna segments differed in size by 55 nucleotides. comparison of the s rna intergenic regions revealed the absence of a stretch of 115 nucleotides within the s rna segment of tyrv-s and, conversely, of 56 nts in that of tyrv-t, apparently a stable geneti ... | 2007 | 16932983 |