| isolation and characterization of a new vesivirus from rabbits. | this report describes the isolation, cdna cloning, complete genome nucleotide sequence, and partial characterization of a new cultivable calicivirus isolated from juvenile feeder european rabbits (oryctolagus cuniculus) showing symptoms of diarrhea. absence of neutralization by type-specific neutralizing antibodies for 40 caliciviruses and phylogenetic sequence comparisons of the open reading frame 1-encoded polyprotein with those of other caliciviruses demonstrate that this new calicivirus is a ... | 2005 | 15901487 |
| structural and functional analysis of virus factories purified from rabbit vesivirus-infected vero cells. | rabbit vesivirus infection induces membrane modifications and accumulation of vesicular structures in the cytoplasm of infected vero cells. crude rav replication complexes (rcs) have been purified and their structural and functional properties have been characterized. we show that calnexin, an er-resident protein, rav non-structural proteins 2ab-, 2c-, 3a-, 3b- and 3cd-like as well as viral rnas co-localize within membranous structures which are able to replicate the endogenous rna templates. th ... | 2008 | 18625274 |
| genomic characterization of novel marine vesiviruses from steller sea lions (eumetopias jubatus) from alaska. | marine vesiviruses were isolated in cell culture from oral and rectal swabs and vesicular fluid from alaskan steller sea lions (ssl; eumetopias jubatus). further characterization by rt-pcr, complete genomic sequencing, and phylogenetic analyses indicated that these viruses are most closely related to the marine vesiviruses, but are distinct viruses and represent two novel genotypes. the complete genome of these two ssl isolates was sequenced after cloning their viral cdna. the genomes were found ... | 2008 | 18765261 |
| role of annexin a2 in cellular entry of rabbit vesivirus. | the mechanisms of calicivirus attachment and internalization are not well understood, mainly due to the lack of a reliable cell-culture system for most of its members. in this study, rabbit vesivirus (rav) virions were shown to bind annexin a2 (anxa2) in a membrane protein fraction from hek293t cells, using a virus overlay protein-binding assay and matrix-assisted laser desorption/ionization time-of-flight analysis. a monoclonal anti-anxa2 antibody and small interfering rna-mediated knockdown of ... | 2009 | 19605586 |