| molecular identification of anisakis species from pleuronectiformes off the portuguese coast. | anisakid nematodes belonging to the anisakis simplex complex are highly prevalent in several fish species off the coast of portugal and are an important zoonotic problem in the iberian peninsula. two reproductively isolated sibling species of the anisakis simplex complex were identified from pleuronectiformes inhabiting the portuguese coast using restriction fragment length polymorphism (rflp). recombinant genotypes corresponding to presumptive anisakis simplex sensu stricto and anisakis pegreff ... | 2006 | 16469172 |
| genetic markers in ribosomal dna for the identification of members of the genus anisakis (nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. | polymerase-chain-reaction-based restriction fragment length polymorphism analysis was performed to establish genetic markers in rdna, for the identification of the three sibling species of the anisakis simplex complex and morphologically differentiated anisakis species, i.e. anisakis physeteris, anisakis schupakovi, anisakis typica and anisakis ziphidarum. different restriction patterns were found between a. simplex sensu stricto and anisakis pegreffii with two of the restriction endonucleases u ... | 2000 | 10704605 |
| integrative taxonomy of anisakidae and raphidascarididae (nematoda) in paralichthys patagonicus and xystreurys rasile (pisces: teleostei) from brazil. | thirty-six paralichthys patagonicus and 30 xystreurys rasile were collected in the state of rio de janeiro, brazil to investigate the presence of anisakid and raphidascaridid nematodes. anisakis typica, terranova sp., contracaecum sp., hysterothylacium deardorffoverstreetorum, and raphidascaris sp. were identified using integrative taxonomy of morphological and genetic data. morphological and morphometric analysis was conducted using bright field microscopy with scanning electron microscopy for ... | 2016 | 27491056 |
| identification of anisakis species (nematoda: anisakidae) in marine fish hosts from papua new guinea. | the third-stage larvae of several genera of anisakid nematodes are important etiological agents for zoonotic human anisakiasis. the present study investigated the prevalence of potentially zoonotic anisakid larvae in fish collected on the coastal shelves off madang and rabaul in papua new guinea (png) where fish represents a major component of the diet. nematodes were found in seven fish species including decapterus macarellus, gerres oblongus, pinjalo lewisi, pinjalo pinjalo, selar crumenophtha ... | 2013 | 23290280 |
| occurrence of ascaridoid nematodes in selected edible fish from the persian gulf and description of hysterothylacium larval type xv and hysterothylacium persicum n. sp. (nematoda: raphidascarididae). | despite several reports on the presence of the potentially zoonotic nematodes among edible fishes in the persian gulf, there is still no study on the specific identification of these parasites or their genetic characterisation. in the present study, a total of 600 fish belonging to five popular species of fish in the region, including otolithes ruber, psettodes erumei, saurida tumbil, scomberomorus commerson and sphyraena jello were examined for infection with nematode parasites. detailed micros ... | 2016 | 27449722 |
| ascaridoid parasites infecting in the frequently consumed marine fishes in the coastal area of china: a preliminary investigation. | marine fishes represent the important components of the diet in the coastal areas of china and they are also natural hosts of various parasites. however, to date, little is known about the occurrence of ascaridoid parasites in the frequently consumed marine fishes in china. in order to determine the presence of ascaridoid parasites in the frequently consumed marine fishes in the coastal town huizhou, guangdong province, china, 211 fish representing 45 species caught from the south china sea (off ... | 2016 | 26546570 |
| molecular identification of anisakis and hysterothylacium larvae in marine fishes from the east china sea and the pacific coast of central japan. | anisakiasis is a human disease caused by the accidental ingestion of larvae belonging to the family anisakidae. three fish species, the small yellow croaker pseudosciaena polyactis, the mackerel pneumatophorus japonicus and the hairtail trichiurus haumela are important source for food products in the east china sea. the prevalence and the identification of anisakidae larvae in these fishes will benefit the prevention and control of anisakiasis. in this study, fish samples were obtained from fish ... | 2015 | 25617845 |
| anisakis pegreffii larvae in sea eels (astroconger myriaster) from the south sea, republic of korea. | anisakis simplex sensu stricto (s.s.), anisakis pegreffii, anisakis berlandi (=a. simplex sp. c), and anisakis typica are the 4 major species of anisakis type i larvae. in the republic of korea (korea), a. pegreffii, a. berlandi, and a. typica larvae in fish hosts has seldom been documented. in this study, molecular analysis was performed on anisakis larvae from the sea eels (astroconger myriaster), the major source of human anisakiasis in korea, collected from tongyeong city, a southern coastal ... | 2015 | 26174831 |
| simultaneous detection and prevalence of allergens in anisakis species isolated from marine fishes. | this study was conducted to develop a multiplex reverse transcription (rt) pcr for the detection of anisakis allergens and to investigate the relationship between allergen profiles and anisakid larvae isolated from scomber japonicus, trichiurus lepturus, and conger myriaster in korea. the species of anisakis was determined using anisakis pegreffii-specific pcr and restriction fragment length polymorphism analysis. the prevalence and profiles of five ani s allergens were examined by multiplex rtp ... | 2016 | 27296426 |
| first record of the anisakid nematode anisakis nascettii in the gervais' beaked whale mesoplodon europaeus from brazil. | anisakid nematodes are parasites with an indirect life cycle, involving many hosts, including cetaceans that act as definitive hosts. identification at species level is crucial for a better understanding of the epidemiology and ecology of these parasites, but an accurate diagnosis based only on morphology is difficult. in brazil, genetic characterization of anisakids is still rare, with anisakis typica and a. physeteris being the only two species identified by genetic markers until now. the defi ... | 2016 | 25348888 |
| occurrence and prevalence of fish-borne anisakis larvae in the spotted mackerel scomber australasicus from taiwanese waters. | anisakid nematodes have been found in a variety of marine fishes throughout the world and they are known to cause anisakiasis in human hosts. the present study investigated the prevalence of potentially zoonotic anisakid larvae in spotted mackerel caught from taiwanese waters where fish represents an important food sources. anisakis third-stage larvae (l3, n=502) were isolated from 250 spotted mackerel scomber australasicus. anisakis l3 larvae were divided morphologically into two types, anisaki ... | 2015 | 25733490 |
| scavenging hagfish as a transport host of anisakid nematodes. | hagfish are the most primitive craniates and scavengers, feeding on dead organisms as well as fish and invertebrates. hagfish play an important ecological role in recycling nutrients, helping to recycle biomass from the upper water column. we investigated 265 specimens of four hagfish species, including eptatretus burgeri, eptatretus yangi, eptatretus sheni and eptatretus taiwanae from northeastern taiwanese waters of the northwestern pacific from november 2013 to june 2014. eight species of ani ... | 2016 | 26872923 |
| Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters. | The blackspot seabream, Pagellus bogaraveo, is a sparid fish of great economic importance in the northeast Atlantic. The main aim of this work was to assess the infection levels and diversity of anisakid nematodes parasitizing P. bogaraveo from Portuguese waters. The anisakid larvae were identified by polymerase chain reaction-restriction fragment length polymorphism analysis and ten different patterns were observed, four of which were not previously reported in the literature. Moreover, several ... | 2011 | 22139402 |
| morphological and molecular characterization of anisakis larvae (nematoda: anisakidae) in beryx splendens from japanese waters. | the third-stage (l3) larvae of anisakis, which are the etiological agents of human anisakiasis, have been categorized morphologically into anisakis type i larvae and anisakis type ii larvae. genetic analysis has allowed easy identification of these larvae: anisakis type i larvae include the species anisakis simplex sensu stricto, anisakis pegreffii, anisakis simplex c, anisakis typica, anisakis ziphidarum, and anisakis nascettii, whereas anisakis type ii larvae include the species anisakis physe ... | 2011 | 21377540 |
| genetic analysis of anisakis typica (nematoda: anisakidae) from cetaceans of the northeast coast of brazil: new data on its definitive hosts. | anisakids from 5 different species of cetacean, kogia breviceps, peponocephala electra, stenella clymene, stenella longirostris and steno bredanensis, were submitted to genetic analysis. adults and larvae fixed in ethanol-formalin-acetic acid or in 70% ethanol for periods ranging from 10 months to 10 years were isolated from 9 cetaceans stranded on ceará coast, northeast brazil. the 18s rdna gene, its1, and specific anisakis typica its regions were amplified by pcr. 18s rdna and its1 region conf ... | 2011 | 21324600 |
| molecular characterization and phylogeny of anisakid nematodes from cetaceans from southeastern atlantic coasts of usa, gulf of mexico, and caribbean sea. | in the present study, 407 anisakid nematodes, collected from 11 different species of cetaceans of the families delphinidae, kogiidae, physeteridae, and ziphiidae, from the southeastern atlantic coasts of usa, the gulf of mexico, and the caribbean sea, were examined morphologically and genetically characterized by pcr restriction fragment length polymorphism to identify them to species level, assess their relative frequencies in definitive hosts, and determine any host preference. sequence data f ... | 2011 | 21212982 |
| molecular identification of anisakis type i larvae isolated from hairtail fish off the coasts of taiwan and japan. | anisakid nematodes are known to cause the zoonotic disease, anisakiasis, through the consumption of raw or undercooked fish. the parasites most frequently associated with the disease in humans are categorized as anisakis type i, which comprise several species of the genus anisakis. the larvae show primitive forms and lack the detailed morphological characteristics required for precise species identification. thus, molecular characterization is necessary for determining the species of anisakis ty ... | 2010 | 20826034 |
| genetic characterization of anisakis typica and anisakis physeteris from marine mammals and fish from the atlantic ocean off brazil. | until now, anisakis typica has been the sole anisakid identified by means of genetic markers from the atlantic ocean off brazil. in this study we developed, and applied to larvae and adults, an a. typica-specific molecular identification protocol based on the complete intergenic transcribed spacer (its) region. anisakids from the dolphin sotalia guianensis and from the frigate tuna, auxis thazard, caught off the coast of brazil, were processed using two dna extraction methods. molecular diagnosi ... | 2009 | 19651475 |
| additional records of metazoan parasites from caribbean marine mammals, including genetically identified anisakid nematodes. | studies of marine mammal parasites in the caribbean are scarce. an assessment for marine mammal endo- and ectoparasites from puerto rico and the virgin islands, but extending to other areas of the caribbean, was conducted between 1989 and 1994. the present study complements the latter and enhances identification of anisakid nematodes using molecular markers. parasites were collected from 59 carcasses of stranded cetaceans and manatees from 1994 to 2006, including globicephala macrorhynchus, kogi ... | 2009 | 19582477 |
| specific pcr assays for the identification of common anisakid nematodes with zoonotic potential. | based on the sequences of the internal transcribed spacers (its-1 and its-2) of nuclear ribosomal dna (rdna) for six taxa of anisakids, namely, anisakis simplex (s.s.), anisakis typica, anisakis pegreffii, hysterothylacium aduncum, hysterothylacium sp, and contracaccum osculatum c, specific primers were designed in the its-1 and/or its-2 for each of the six anisakid taxa. these specific primers were used to develop polymerase chain reaction (pcr) tools for the identification of these anisakid ta ... | 2008 | 18758823 |
| occurrence and molecular identification of anisakis spp. from the north african coasts of mediterranean sea. | larval forms of the genus anisakis were reported infecting several fish species from the north african coasts of central mediterranean sea. polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis was used to investigate the occurrence of larval forms of different anisakis species in teleost fishes and squid from north african coasts of the mediterranean sea and to establish the geographical and host range of these parasites in this area. a total of 282 anisakis lar ... | 2008 | 18026753 |
| anisakis (nematoda: ascaridoidea) from indonesia. | despite indonesia's high marine biodiversity, there is a lack of information regarding fish parasites in indonesian waters. during a sampling of 136 teleost species from indonesian waters, 22 of them were infected with larvae of anisakis dujardin, 1845, a genus with zoonotic potential. we genetically identified 118 worms, provide a revision of all available sequences of the its-1-5.8s-its-2 marker from indonesian anisakis in genbank (n = 125), and establish 16 new host records. so far, 53 indone ... | 2017 | 28262635 |
| molecular characterization of larval anisakid nematodes from marine fishes of madeira by a pcr-based approach, with evidence for a new species. | one-hundred and fifteen anisakid larvae from 3 different fish hosts, aphanopus carbo, scomber japonicus, and trachurus picturatus, caught in madeiran waters, were identified by pcr-rflp. three distinct species were identified in a. carbo, namely anisakis simplex sensu srricto, anisakis pegreffii, and anisakis ziphidarum; 5 in s. japonicus, i.e., a. simplex s.s., a. pegreffii, anisakis physeteris, anisakis typica, and a. ziphidarum; and 3 in t. picturatus, i.e., a. simplex s.s., a. pegreffii, and ... | 2005 | 16539027 |
| genetic markers in the study of anisakis typica (diesing, 1860): larval identification and genetic relationships with other species of anisakis dujardin, 1845 (nematoda: anisakidae). | genetic variation at 21 gene-enzyme systems was studied in a sample of an adult population of anisakis typica (diesing, 1860) recovered in the dolphin sotalia fluviatilis from the atlantic coast of brazil. the characteristic alleles, detected in this population, made it possible to identify as a. typica, anisakis larvae with a type i morphology (sensu berland, 1961) from various fishes: thunnus thynnus and auxis thazard from brazil waters, trachurus picturatus and scomber japonicus from madeiran ... | 2002 | 11912342 |
| molecular and morphological characterization of anisakid nematode larvae from the sandperches pseudopercis numida and pinguipes brasilianus (perciformes: pinguipedidae) off brazil. | morphological and genetic data on the larvae of anisakidae parasitizing the sandperches pseudopercis numida and pinguipes brasilianus were recorded from cabo frio, state of rio de janeiro, brazil (23°s, 42°w). twenty-five specimens of p. numida and 25 specimens of p. brasilianus were necropsied. the 352 larvae collected included 1 anisakis sp., 344 hysterothylacium sp., and 7 raphidascaris sp. we present mtdna sequence data for anisakis sp. and hysterothylacium sp., and we sequenced the its ribo ... | 2015 | 25885554 |
| occurrence and molecular identification of anisakis dujardin, 1845 from marine fish in southern makassar strait, indonesia. | anisakis spp. (nematoda: anisakidae) parasitize a wide range of marine animals, mammals serving as the definitive host and different fish species as intermediate or paratenic hosts. in this study, 18 fish species were investigated for anisakis infection. katsuwonus pelamis, euthynnus affinis, caranx sp., and auxis thazard were infected with high prevalence of anisakis type i, while cephalopholis cyanostigma and rastrelliger kanagurta revealed low prevalence. the mean intensity of anisakis larvae ... | 2014 | 24623876 |
| gastrointestinal parasites of free-living indo-pacific bottlenose dolphins (tursiops aduncus) in the northern red sea, egypt. | the present study represents the first report on the gastrointestinal parasite fauna infecting the free-living and alive indo-pacific bottlenose dolphins (tursiops aduncus) inhabiting waters of the red sea at hurghada, egypt. a total of 94 individual faecal samples of the examined bottlenose dolphins were collected during several diving expeditions within their natural habitats. using classical parasitological techniques, such as sodium acetate acetic acid formalin method, carbol fuchsin-stained ... | 2014 | 24477747 |
| identification and genetic characterization of anisakis larvae from marine fishes in the south china sea using an electrophoretic-guided approach. | from 35 species of marine fishes (n = 327) from the south china sea, 237 nematode larvae were collected and identified morphologically as anisakis. genomic dna was isolated from each larva and subjected to pcr-based rflp and targeted sequencing of a nuclear ribosomal dna region between the 3'-end of the small subunit and 5'-end of the large subunit of the rrna genes (= internal transcribed spacers, its+). four different rflp profile combinations (sets) were detected for all restriction endonucle ... | 2013 | 23335012 |
| an environmental assessment of the parasite fauna of the reef-associated grouper epinephelus areolatus from indonesian waters. | sixty epinephelus areolatus were examined for metazoan fish parasites in indonesia, off segara anakan lagoon, java and in balinese waters. the study revealed 21 different parasite species, and 14 new host and locality records. the anisakid nematodes anisakis typica and, for the first time in indonesia, anisakis sp. hc-2005 were identified by using molecular methods. ecological parameters were calculated for both sites off the anthropogenically influenced segara anakan lagoon and the relatively u ... | 2014 | 23095724 |
| morphological and molecular diagnosis of anisakid nematode larvae from cutlassfish (trichiurus lepturus) off the coast of rio de janeiro, brazil. | anisakid nematode larvae from trichiurus lepturus off coast of rio de janeiro were studied using light, laser confocal and scanning electron microscopy, in addition to a molecular approach. mitochondrial cytochrome c-oxidase subunit 2 (mtdna cox-2), partial 28s (lsu) and internal transcribed spacers (its-1, 5.8s, its-2) of ribosomal dna were amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships between the nematode taxa. the morphological and gene ... | 2012 | 22792329 |
| molecular characterisation of anisakidae larvae from fish in adriatic sea. | in the present study, anisakids from: tuna (thunnus thynnus) fattened in the croatian farm in middle adriatic sea, three different feral fish species caught near tuna farm (trachurus trachurus, scomber japonicus and oblada melanura) and fish marketed in croatia (t. trachurus) were analysed by morphology and molecular methods. larvae were identified to the species level by pcr restriction fragment length polymorphism and characterised by sequencing of nuclear (internal transcribed spacer) and mit ... | 2012 | 22983167 |
| molecular identification of anisakis species (nematoda: anisakidae) from marine fishes collected in turkish waters. | anisakid nematodes are important etiological agents for zoonotic human anisakiasis (or anisakidosis). these parasites in the turkish waters still remain unexplored. this study aims the molecular identification of anisakis species in turkey's coast from black, aegean and mediterranean sea and specifically to screen for zoonotic species in commonly commercialized a total of 1145 fish belonging to 31 different species using both polymerase chain reaction-restriction fragment length polymorphism ana ... | 2014 | 24485564 |
| identification of anisakid nematodes with zoonotic potential from europe and china by single-strand conformation polymorphism analysis of nuclear ribosomal dna. | using genetic markers defined previously in the second internal transcribed spacer (its-2) of nuclear ribosomal dna (rdna), isotopic, and non-isotopic polymerase-chain-reaction-coupled single-strand conformation polymorphism (sscp) were utilized to identify each of three anisakid species [anisakis simplex (s.l.), contracaecum osculatum (s.l.), and hysterothylacium aduncum] from different host species and geographical locations in poland and sweden. while subtle microheterogeneity was observed wi ... | 2007 | 17694403 |
| occurrence of anisakis (nematoda: anisakidae) larvae in unusual hosts in southern hemisphere. | nematodes belonging to the genus anisakis are important parasites due to their abundance in seafood and health impacts on humans. in the present study anisakis larvae were found in a number of uncommon hosts including the grey petrel, procellaria cinerea, the little penguin, eudyptula minor, blue-lipped sea krait, laticauda laticaudata and spinner shark, carcharhinus brevipinna. morphological examination showed nematodes in these animals are anisakis larval type i. genetic characterisation sugge ... | 2017 | 28797592 |
| integrative taxonomy of anisakid nematodes in stranded cetaceans from brazilian waters: an update on parasite's hosts and geographical records. | anisakidae are represented mainly by species of anisakis dujardin, 1845, pseudoterranova krabbe, 1878, and contracaecum railliet and henry, 1913. integrative taxonomy, based on morphological and genetic approaches, can provide a precise identification of species, increasing the knowledge of their systematics, biology, and evolution. in this study, integrative taxonomy was applied in order to identify, at generic and/or specific taxonomic level, 245 anisakids from 63 cetaceans of 12 different spe ... | 2017 | 28975458 |