| genetic and ecological data on the anisakis simplex complex, with evidence for a new species (nematoda, ascaridoidea, anisakidae). | isozyme analysis at 24 loci was carried out on anisakid nematodes of the anisakis simplex complex, recovered from various intermediate/paratenic (squid, fish) and definitive (marine mammals) hosts from various parts of the world. a number of samples were found to belong to a. simplex sensu stricto and anisakis pegreffii, widely extending the geographic ranges and the number of hosts of these 2 species. in addition, a new distinct gene pool was detected, showing different alleles with respect to ... | 1997 | 9194819 |
| genetic markers in ribosomal dna for the identification of members of the genus anisakis (nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. | polymerase-chain-reaction-based restriction fragment length polymorphism analysis was performed to establish genetic markers in rdna, for the identification of the three sibling species of the anisakis simplex complex and morphologically differentiated anisakis species, i.e. anisakis physeteris, anisakis schupakovi, anisakis typica and anisakis ziphidarum. different restriction patterns were found between a. simplex sensu stricto and anisakis pegreffii with two of the restriction endonucleases u ... | 2000 | 10704605 |
| diagnosis of a case of gastric anisakidosis by pcr-based restriction fragment length polymorphism analysis. | a set of genetic markers, based on pcr-rflps of three diagnostic restriction enzymes (hhal, hinfl and taql), which proved to be suitable for the identification of the species of the genus anisakis, was used for the first molecular identification of a larva obtained by endoscopy in a case of gastric anisakidosis, in a 51 year old woman from southern italy. the analysis of the restriction profiles obtained allowed the larva to be identified as anisakis pegreffii, one of the three sibling species o ... | 1999 | 10870568 |
| ascaridoidea: a simple dna assay for identification of 11 species infecting marine and freshwater fish, mammals, and fish-eating birds. | eleven species belonging to superfamily ascaridoidea, which infect marine and freshwater fish, mammals, and fish-eating birds, were analyzed using a pcr-rflp method. the following species were investigated: anisakis pegreffi, a. physeteris, and a. simplex (parasites of fish and mammals), contracaecum osculatum, c. radiatum, and c. rudolphi (parasites of mammals and fish-eating birds), hysterothylacium aduncum (a parasite of fish), porrocaecum angusticolle, p. crassum, p. depressum, and p. ensica ... | 2002 | 12243736 |
| occurrence of recombinant genotypes of anisakis simplex s.s. and anisakis pegreffii (nematoda: anisakidae) in an area of sympatry. | the anisakid nematode populations collected from fish and stranded cetaceans along from iberian peninsula waters were morphologically identified as corresponding to the anisakis simplex complex. in order to realise their molecular identification and to analyse the extent of genetic variation, the entire its (its1, 5.8s rdna gene and its2) and the mitochondrial small subunit of rrna were pcr-amplified and sequenced. digestions of the amplified its region with hinfi and hhai allowed the identifica ... | 2003 | 14522181 |
| molecular characterization of anisakis pegreffii larvae in pacific cod in japan. | it is now recognized that the morphospecies anisakis simplex is not a single species but a complex composed of three sibling species, a. simplex sensu stricto, a. pegreffii and a. simplex c. in japan, a. simplex-like larvae have been isolated from a variety of fish and humans, but the larvae collected have been identified as a. simplex by only light microscopy. therefore, the epidemiology of the a. simplex complex, composed of three sibling species, is still unclear in japan. in the present stud ... | 2005 | 16336712 |
| molecular identification of anisakis species from pleuronectiformes off the portuguese coast. | anisakid nematodes belonging to the anisakis simplex complex are highly prevalent in several fish species off the coast of portugal and are an important zoonotic problem in the iberian peninsula. two reproductively isolated sibling species of the anisakis simplex complex were identified from pleuronectiformes inhabiting the portuguese coast using restriction fragment length polymorphism (rflp). recombinant genotypes corresponding to presumptive anisakis simplex sensu stricto and anisakis pegreff ... | 2006 | 16469172 |
| molecular characterization of larval anisakid nematodes from marine fishes of madeira by a pcr-based approach, with evidence for a new species. | one-hundred and fifteen anisakid larvae from 3 different fish hosts, aphanopus carbo, scomber japonicus, and trachurus picturatus, caught in madeiran waters, were identified by pcr-rflp. three distinct species were identified in a. carbo, namely anisakis simplex sensu srricto, anisakis pegreffii, and anisakis ziphidarum; 5 in s. japonicus, i.e., a. simplex s.s., a. pegreffii, anisakis physeteris, anisakis typica, and a. ziphidarum; and 3 in t. picturatus, i.e., a. simplex s.s., a. pegreffii, and ... | 2005 | 16539027 |
| molecular identification of anisakis simplex sensu stricto and anisakis pegreffii (nematoda: anisakidae) from fish and cetacean in japanese waters. | parasites morphologically consistent with anisakis simplex sensu lato collected from the coast of japan and western north pacific ocean were examined by pcr-rflp of the its region (its1, 5.8 subunit rrna gene and its2) and mtdna cox1. the rflp patterns of rdna generated by hinfi and hhai showed that 100% of the larvae collected from hokkaido and 94% of adults collected from western north pacific ocean were identified as a. simplex sensu stricto. on the other hand, 97% of the larvae collected fro ... | 2006 | 16942906 |
| the specific identification of anisakid larvae from fishes from the yellow sea, china, using mutation scanning-coupled sequence analysis of nuclear ribosomal dna. | nematodes of the family anisakidae parasitize fish, mammals, birds and reptiles, with the larval stages of some species causing severe clinical disease in humans. therefore, the accurate identification of anisakid nematodes is a key component of disease surveillance and control. an epidemiological survey of 123 fishes comprising eight different species from the yellow sea in china revealed that more than 25% of fish were infected with the larvae of anisakids, 200 third-stage larvae (l3s) were co ... | 2007 | 17604951 |
| identification of anisakid nematodes with zoonotic potential from europe and china by single-strand conformation polymorphism analysis of nuclear ribosomal dna. | using genetic markers defined previously in the second internal transcribed spacer (its-2) of nuclear ribosomal dna (rdna), isotopic, and non-isotopic polymerase-chain-reaction-coupled single-strand conformation polymorphism (sscp) were utilized to identify each of three anisakid species [anisakis simplex (s.l.), contracaecum osculatum (s.l.), and hysterothylacium aduncum] from different host species and geographical locations in poland and sweden. while subtle microheterogeneity was observed wi ... | 2007 | 17694403 |
| multiplex pcr for the identification of anisakis simplex sensu stricto, anisakis pegreffii and the other anisakid nematodes. | a multiplex pcr method was established for the rapid identification of anisakis simplex sensu stricto, a. pegreffii, a. physeteris, pseudoterranova decipiens, contracaecum osculatum and hysterothylacium aduncum. the sequence alignment of the internal transcribed spacer 1 region (its-1) between a. simplex s. str. and a. pegreffii showed a high degree of similarity, but only two c-t transitions were observed. to differentiate a. simplex s. str. from a. pegreffii, an intentional mismatch primer wit ... | 2008 | 17962067 |
| occurrence and molecular identification of anisakis spp. from the north african coasts of mediterranean sea. | larval forms of the genus anisakis were reported infecting several fish species from the north african coasts of central mediterranean sea. polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis was used to investigate the occurrence of larval forms of different anisakis species in teleost fishes and squid from north african coasts of the mediterranean sea and to establish the geographical and host range of these parasites in this area. a total of 282 anisakis lar ... | 2008 | 18026753 |
| application of the pcr-sequence-specific primers for the discrimination among larval anisakis simplex complex. | the fish-borne zoonotic nematode, anisakis simplex, is not a single specie but a complex composed of three sibling species, a. simplex sensu stricto, anisakis pegreffii, and a. simplex c. discrimination among these sibling species have been performed by polymerase chain reaction (pcr)-restriction fragment length polymorphism or sequencing analysis of the internal transcribed spacer (its) region of ribosomal dna (rdna). in the present study, pcr-sequence-specific primers were applied for the disc ... | 2008 | 18239942 |
| morphological differences between larvae and in vitro-cultured adults of anisakis simplex (sensu stricto) and anisakis pegreffii (nematoda: anisakidae). | proper identification of anisakis species infecting host fishes is very important to both human health and fish disease diagnosis. the foremost problem in the identification of anisakis larvae in fishes is that l3 larvae cannot be easily differentiated morphologically, especially between a. simplex (sensu stricto) (s.s.) (rudolphi, 1809) and a. pegreffii campana-rouget et biocca, 1955. instead, molecular means such as allozyme, mitochondrial dna (mtdna) cox2 region and polymerase chain reaction- ... | 2008 | 18644463 |
| specific pcr assays for the identification of common anisakid nematodes with zoonotic potential. | based on the sequences of the internal transcribed spacers (its-1 and its-2) of nuclear ribosomal dna (rdna) for six taxa of anisakids, namely, anisakis simplex (s.s.), anisakis typica, anisakis pegreffii, hysterothylacium aduncum, hysterothylacium sp, and contracaccum osculatum c, specific primers were designed in the its-1 and/or its-2 for each of the six anisakid taxa. these specific primers were used to develop polymerase chain reaction (pcr) tools for the identification of these anisakid ta ... | 2008 | 18758823 |
| anisakis pegreffi etiological agent of gastric infections in two italian women. | two cases of gastric anisakiasis have been documented in two italian women who had consumed raw anchovies (engraulis encrasicolus). the first patient was a 49-year-old woman presenting with epigastric pain and bloody vomiting after ingestion of marinated (vinegar) raw anchovies. during the esophagogastroduodenoscopy (egds) a white color worm was detected and extracted from cardia by means of biopsy forceps. the second patient was a 59-year-old woman with irritable bowel syndrome and gastritis, w ... | 2009 | 19642920 |
| multiple primer pcr for the identification of anisakid nematodes from taiwan strait. | there were six major larval anisakid species found in commercial marine fishes caught in the minnan fishing ground in the taiwan strait: anisakis physeteris, anisakis pegreffii, raphidascaris trichiuri, contracaecum aduncum, contracaecum muraenesoxi, contracaecum sp. for rapid identification of the parasite species above, a single and a multiple primer pcr (multiplex pcr) method, using specific primers based on aligned sequences of the internal transcribed spacer its-1, 5.8s, and its-2 of nuclea ... | 2010 | 19751726 |
| risk factors for human anisakis infection and association between the geographic origins of scomber japonicus and anisakid nematodes. | it has been reported that nearly all cases of anisakiasis in japan are caused by anisakis simplex sensu stricto. to elucidate this further, we investigated the presence of anisakis type i larvae and pseudoterranova decipiens in 218 scomber japonicus fish collected from the seas of japan. anisakis type i larvae were detected in 74.3% (162/218) of the fish, and 99.8% of the anisakis type i larvae comprised a. simplex sensu stricto and anisakis pegreffii. molecular identification techniques were us ... | 2010 | 19892425 |
| elucidating the identity of anisakis larvae from a broad range of marine fishes from the yellow sea, china, using a combined electrophoretic-sequencing approach. | larval nematodes were collected from marine fishes from the yellow sea, china. specimens (n=1731) of anisakis type i from 311 fishes (representing 40 species) were each identified based on morphological characters. from the genomic dna from individual specimens, a region of nuclear ribosomal dna was amplified by pcr, followed by digestion with restriction endonuclease hinfi, taqi or hhai. subsequently, the its-1 and its-2 regions of selected samples were sequenced. the results revealed three spe ... | 2010 | 20108262 |
| anisakis pegreffii-induced airway hyperresponsiveness is mediated by gamma interferon in the absence of interleukin-4 receptor alpha responsiveness. | infection with the fish parasite anisakis following exposure to contaminated fish can lead to allergic reactions in humans. the present study examined the immunological mechanisms underlying the development of allergic airway inflammation in mice after different routes of sensitization to anisakis. wild-type and interleukin-4 receptor alpha (il-4ralpha)-deficient balb/c mice were sensitized intraperitoneally with live or heat-killed anisakis larvae or by intranasal administration of an anisakis ... | 2010 | 20605987 |
| genetic variability of anisakis simplex s.s. parasitizing european hake (merluccius merluccius) in the little sole bank area in the northeast atlantic. | in this study, we researched the presence of anisakids in specimens of merluccius merluccius caught in the area of little sole bank, in the northeast atlantic, and found that 100% of the european hake examined were infected and showed high average values of abundance (976.88) and intensity (976.88). the larvae were identified in morphological terms as morphotype type i and in molecular terms as anisakis simplex s.s via polymerase chain reaction (pcr) restriction fragment length polymorphism of t ... | 2010 | 20694563 |
| molecular identification of anisakis type i larvae isolated from hairtail fish off the coasts of taiwan and japan. | anisakid nematodes are known to cause the zoonotic disease, anisakiasis, through the consumption of raw or undercooked fish. the parasites most frequently associated with the disease in humans are categorized as anisakis type i, which comprise several species of the genus anisakis. the larvae show primitive forms and lack the detailed morphological characteristics required for precise species identification. thus, molecular characterization is necessary for determining the species of anisakis ty ... | 2010 | 20826034 |
| molecular identification and pathology of anisakis pegreffii (nematoda: anisakidae) infection in the mediterranean loggerhead sea turtle (caretta caretta). | nematodes of genus anisakis spp. parasitize a wide range of marine hosts with marine mammals (mainly cetaceans) serving as definitive hosts, while fish, squid and other invertebrates serve as paratenic or intermediate hosts. sea turtles can act as accidental or paratenic hosts for anisakis spp. larvae, harbouring third-stage larvae unable to complete their life cycle in an ectothermic vertebrate. post-mortem examination of 96 loggerhead sea turtles (caretta caretta) stranded along the italian co ... | 2010 | 20850929 |
| anisakis pegreffii: a quantitative fluorescence pcr assay for detection in situ. | to facilitate improved diagnosis and detection of the third stage larva (l3) of anisakis pegreffii from the minnan-taiwan bank fishing ground in taiwan strait, a real-time pcr method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (its-2) of this parasite. the real-time pcr assay was capable of detecting 1/3 of a single l3 in 30 mg of marine fish tissue, and also exhibited a high level of specificity for a. pegreffii, no f ... | 2010 | 21110972 |
| experimental challenge of anisakis simplex sensu stricto and anisakis pegreffii (nematoda: anisakidae) in rainbow trout and olive flounder. | the third-stage larvae of anisakis simplex sensu lato (s.l.) are found in many marine fishes. to ensure food safety, it is important to determine whether these larvae are present in the body muscle of commercial fish species. however, there is little information regarding the tissue specificity of anisakis and two of its sibling species, a. simplex sensu stricto (s.s.) and anisakis pegreffii, that are common in marine fish in japanese waters. we orally challenged rainbow trout (oncorhynchus myki ... | 2010 | 21122822 |
| molecular characterization and phylogeny of anisakid nematodes from cetaceans from southeastern atlantic coasts of usa, gulf of mexico, and caribbean sea. | in the present study, 407 anisakid nematodes, collected from 11 different species of cetaceans of the families delphinidae, kogiidae, physeteridae, and ziphiidae, from the southeastern atlantic coasts of usa, the gulf of mexico, and the caribbean sea, were examined morphologically and genetically characterized by pcr restriction fragment length polymorphism to identify them to species level, assess their relative frequencies in definitive hosts, and determine any host preference. sequence data f ... | 2011 | 21212982 |
| insight into the short-finned squid illex coindetii (cephalopoda: ommastrephidae) feeding ecology: is there a link between helminth parasites and food composition? | squids are especially frequent as paratenic hosts of helminth parasites, particularly to those that have elasmobranchs and mammals as final hosts. among those parasite species, anisakid nematode larvae and cestode plerocercoids are most effectively transferred through the trophic chain by oegopsid squids. a total of 439 short-finned squids, illex coindetii (245 males, 190 females and 4 unsexed) were sampled in the central part of the eastern adriatic sea in order to assess their helminth compone ... | 2010 | 21348607 |
| morphological and molecular characterization of anisakis larvae (nematoda: anisakidae) in beryx splendens from japanese waters. | the third-stage (l3) larvae of anisakis, which are the etiological agents of human anisakiasis, have been categorized morphologically into anisakis type i larvae and anisakis type ii larvae. genetic analysis has allowed easy identification of these larvae: anisakis type i larvae include the species anisakis simplex sensu stricto, anisakis pegreffii, anisakis simplex c, anisakis typica, anisakis ziphidarum, and anisakis nascettii, whereas anisakis type ii larvae include the species anisakis physe ... | 2011 | 21377540 |
| first molecular identification of the zoonotic parasite anisakis pegreffii (nematoda: anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in italy. | anisakiasis is an important fish-borne zoonosis provoked by larval stages of nematodes belonging to the genus anisakis. the detection and identification of human infections is difficult. this is due to: a) the low specificity of the clinical features and symptomatology related to human infections; b) the paucity of diagnostic features of larvae found in granulomatous lesions characteristic of "invasive anisakiasis"; and c) the lack morphological characters diagnostic at the specific level when l ... | 2011 | 21453522 |
| molecular characterization of anisakis larvae from fish caught off sardinia. | abstract anisakis spp. larvae are parasitic, and potentially zoonotic, nematodes transmitted by marine fish and cephalopods, which are the main intermediate hosts of the third larval stage. the accidental consumption of infected raw or poorly cooked fish may cause gastroenteric diseases and allergies in humans. the aim of the present study was to use pcr-rflp to define the occurrence, species variability, and host preferences of anisakis spp. larvae in fish caught off the coast of sardinia. necr ... | 2011 | 21501045 |
| population structure of three species of anisakis nematodes recovered from pacific sardine (sardinops sagax) distributed throughout the california current system. | abstract members of the anisakidae are known to infect over 200 pelagic fish species and have been frequently used as biological tags to identify fish populations. despite information on the global distribution of anisakis species, there is little information on the genetic diversity and population structure of this genus, which could be useful in assessing the stock structure of their fish hosts. from 2005 through 2008, 148 larval anisakids were recovered from pacific sardine (sardinops sagax) ... | 2011 | 21506810 |
| Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters. | The blackspot seabream, Pagellus bogaraveo, is a sparid fish of great economic importance in the northeast Atlantic. The main aim of this work was to assess the infection levels and diversity of anisakid nematodes parasitizing P. bogaraveo from Portuguese waters. The anisakid larvae were identified by polymerase chain reaction-restriction fragment length polymorphism analysis and ten different patterns were observed, four of which were not previously reported in the literature. Moreover, several ... | 2011 | 22139402 |
| molecular characterization of anisakid nematode larvae from 13 species of fish from western australia. | this study characterized anisakid nematodes in estuarine and near-shore species of fish in southern western australia. a total of 108 fish representing 13 species were examined for anisakid larvae. for the molecular characterization of anisakid larvae (n=218), we used pcr-coupled mutation scanning-sequencing-phylogenetic analyses of sequence variation in the internal transcribed spacers of nuclear ribosomal dna. with the exception of sillaginoides punctatus and sillago schomburgkii, all the fish ... | 2012 | 23353682 |
| scavenging hagfish as a transport host of anisakid nematodes. | hagfish are the most primitive craniates and scavengers, feeding on dead organisms as well as fish and invertebrates. hagfish play an important ecological role in recycling nutrients, helping to recycle biomass from the upper water column. we investigated 265 specimens of four hagfish species, including eptatretus burgeri, eptatretus yangi, eptatretus sheni and eptatretus taiwanae from northeastern taiwanese waters of the northwestern pacific from november 2013 to june 2014. eight species of ani ... | 2016 | 26872923 |
| occurrence and prevalence of fish-borne anisakis larvae in the spotted mackerel scomber australasicus from taiwanese waters. | anisakid nematodes have been found in a variety of marine fishes throughout the world and they are known to cause anisakiasis in human hosts. the present study investigated the prevalence of potentially zoonotic anisakid larvae in spotted mackerel caught from taiwanese waters where fish represents an important food sources. anisakis third-stage larvae (l3, n=502) were isolated from 250 spotted mackerel scomber australasicus. anisakis l3 larvae were divided morphologically into two types, anisaki ... | 2015 | 25733490 |
| effect of different temperatures on the expression of the newly characterized heat shock protein 90 (hsp90) in l3 of anisakis spp. isolated from scomber australasicus. | anisakid nematodes are distributed worldwide in a wide variety of marine fishes and they are known to cause the zoonotic disease, anisakiasis. the temperature control is commonly applied for prevention and control of anisakiasis. to analyze the cellular response to temperature stress in anisakis, the heat shock protein 90 (hsp90) was chosen in the present study, as it plays a key role in many cellular processes and responds to stress conditions such as heat or cold shock. anisakids were sampled ... | 2014 | 25269988 |
| metazoan parasite infection in the swordfish, xiphias gladius, from the mediterranean sea and comparison with atlantic populations: implications for its stock characterization. | thirteen parasite taxa were identified in the mediterranean swordfish by morphological and genetic/molecular methods. the comparison of the identified parasite taxa and parasitic infection values observed in the mediterranean swordfish showed statistically significant differences with respect to those reported for its atlantic populations. a stepwise linear discriminant analysis of the individual fish examined showed a separation among three groups: one including fish from the mediterranean sea ... | 2014 | 25057787 |
| occurrence of nematodes of the genus anisakis in mediterranean and atlantic fish marketed in sardinia. | anisakiasis is a gastrointestinal fish-borne zoonosis caused by the ingestion of third stage larvae of the genus anisakis. between january and december 2013, 1112 specimens of four commercial fish species (engraulis encrasicolus, merluccius merluccius, scomber colias and trachurus mediterraneus) marketed in sardinia (italy) were examined for anisakis sp. the overall prevalence of anisakis spp larvae was 39.9%, all morphologically identified as type i. scomber colias showed the highest prevalence ... | 2017 | 28299289 |
| anisakis simplex complex: ecological significance of recombinant genotypes in an allopatric area of the adriatic sea inferred by genome-derived simple sequence repeats. | the genus anisakis includes nine species which, due to close morphological resemblance even in the adult stage, have previously caused many issues in their correct identification. recently observed interspecific hybridisation in sympatric areas of two closely related species, anisakis simplex sensu stricto (s.s.) and anisakis pegreffii, has raised concerns whether a f1 hybrid generation is capable of overriding the breeding barrier, potentially giving rise to more resistant/pathogenic strains in ... | 2017 | 28057461 |
| anisakis pegreffii (nematoda: anisakidae) products modulate oxidative stress and apoptosis-related biomarkers in human cell lines. | in countries with elevated prevalence of zoonotic anisakiasis and high awareness of this parasitosis, a considerable number of cases that associate anisakis sp. (nematoda, anisakidae) and different bowel carcinomas have been described. although neoplasia and embedded larvae were observed sharing the common site affected by chronic inflammation, no association between the nematode and malignancy were directly proved. similarly, no data are available about the effect of secretory and excretory pro ... | 2016 | 27887635 |
| survey for the presence of ascaridoid larvae in the cinnamon flounder pseudorhombus cinnamoneus (temminck & schlegel) (pleuronectiformes: paralichthyidae). | the cinnamon flounder pseudorhombus cinnamoneus is a frequently consumed marine fish in china. however, the occurrence of ascaridoid larvae in p. cinnamoneus remains unclear. in the present study, a total of 85 p. cinnamoneus caught from the yellow sea (off shidao, 36°52'57″n, 122°26'42″e) in 2011, which is located between mainland china and the korean peninsula, was investigated for ascaridoid larval infection. four ascaridoid larval types, including anisakis type i of berland (1961), hysteroth ... | 2017 | 27768930 |
| survey on the presence of a. simplex s.s. and a. pegreffii hybrid forms in central-western mediterranean sea. | in this study, 1029 fish and cephalopod samples came from central-western mediterranean (fao 37.1.1 and fao 37.1.3) were analysed for anisakidae larvae research with the aim to identify possible hybridisations between anisakis pegreffii and anisakis simplex s.s. species. a total of 1765 larvae were detected, with prevalence values between 8.1% and 100%. the morphologic analysis revealed characters attributable to morphotype i of anisakis in 98.5% of the examined larvae, while 1.5% belonged to th ... | 2016 | 27568095 |
| first case report of invasive pseudoterranoviasis in italy. | members of the genera anisakis and pseudoterranova are the main causative agents of human anisakidosis: the disease is worldwide distributed, with major impact in countries with a large consumption of raw fish. because of unspecific symptoms and limited diagnostic tools, incidence and burden of disease are probably underestimated. in italy, all human infestations where the etiological agent has been properly identified, have been associated to the parasitic species anisakis pegreffii, the most f ... | 2016 | 27395343 |
| simultaneous detection and prevalence of allergens in anisakis species isolated from marine fishes. | this study was conducted to develop a multiplex reverse transcription (rt) pcr for the detection of anisakis allergens and to investigate the relationship between allergen profiles and anisakid larvae isolated from scomber japonicus, trichiurus lepturus, and conger myriaster in korea. the species of anisakis was determined using anisakis pegreffii-specific pcr and restriction fragment length polymorphism analysis. the prevalence and profiles of five ani s allergens were examined by multiplex rtp ... | 2016 | 27296426 |
| molecular confirmation of anisakis pegreffii as a causative agent of anisakidosis in france. | | 2016 | 27133969 |
| anisakiasis mimics cancer recurrence: two cases of extragastrointestinal anisakiasis suspected to be recurrence of gynecological cancer on pet-ct and molecular biological investigation. | we report two cases of anisakiasis lesions that were initially suspected to be recurrence of gynecological cancer by positron emission tomography-computed tomography (pet-ct). both cases were extragastrointestinal anisakiasis that is very rare. | 2016 | 27112922 |
| no more time to stay 'single' in the detection of anisakis pegreffii, a. simplex (s. s.) and hybridization events between them: a multi-marker nuclear genotyping approach. | a multi-marker nuclear genotyping approach was performed on larval and adult specimens of anisakis spp. (n = 689) collected from fish and cetaceans in allopatric and sympatric areas of the two species anisakis pegreffii and anisakis simplex (s. s.), in order to: (1) identify specimens belonging to the parental taxa by using nuclear markers (allozymes loci) and sequence analysis of a new diagnostic nuclear dna locus (i.e. partial sequence of the ef1 α-1 ndna region) and (2) recognize hybrid categ ... | 2016 | 27046418 |
| a case report of anisakis pegreffii (nematoda, anisakidae) identified from archival paraffin sections of a croatian patient. | this is the first report of anisakiasis in a croatian patient, evidenced from an archival paraffin-embedded and hematoxylin-eosin stained tissue section. anisakiasis has been only suspected in the country based on previously detected anti-anisakis ige seroprevalence in the healthy coastal population, as well as an acute case where pathohistological and serological findings suggested the diseases, but the migrating larva has not been retrieved. | 2016 | 26832924 |
| role of biogenic amines in the post-mortem migration of anisakis pegreffii (nematoda: anisakidae dujardin, 1845) larvae into fish fillets. | infective third-stage larvae (l3) of nematode anisakis spp. have been recognized as one of the major food-borne threats in lightly processed fish products in europe, particularly in the mediterranean region. therefore, the effect of different storage temperatures of fish on larval post-mortem migration from visceral cavity into fillets is an important parameter to take into account when evaluating the risk for consumer safety. the european anchovy (engraulis encrasicolus) were caught during fish ... | 2015 | 26318909 |
| expression patterns and structural modelling of hsp70 and hsp90 in a fish-borne zoonotic nematode anisakis pegreffii. | heat shock proteins (hsps) are essential molecular chaperones that are highly conserved across organisms. they have a pivotal function in responding to thermal stress and are responsible for many cellular functions. here, we aimed to elucidate the possible roles of hsp70 and hsp90 in the life cycle of the parasitic nematode anisakis, particularly third- and fourth-stage larvae, from cold-blooded fish to warm-blooded marine mammals or accidentally to human hosts. we examined the expression profil ... | 2015 | 26215928 |
| anisakis pegreffii larvae in sea eels (astroconger myriaster) from the south sea, republic of korea. | anisakis simplex sensu stricto (s.s.), anisakis pegreffii, anisakis berlandi (=a. simplex sp. c), and anisakis typica are the 4 major species of anisakis type i larvae. in the republic of korea (korea), a. pegreffii, a. berlandi, and a. typica larvae in fish hosts has seldom been documented. in this study, molecular analysis was performed on anisakis larvae from the sea eels (astroconger myriaster), the major source of human anisakiasis in korea, collected from tongyeong city, a southern coastal ... | 2015 | 26174831 |
| macroparasites of allis shad (alosa alosa) and twaite shad (alosa fallax) of the western iberian peninsula rivers: ecological, phylogenetic and zoonotic insights. | samples of anadromous alosa alosa (clupeidae) (n = 163) and alosa fallax (clupeidae) (n = 223), caught in western iberian peninsula rivers from 2008 to 2013, were examined for buccal, branchial and internal macroparasites, which were identified using morphological and molecular methods. alosa alosa were infected with anisakis simplex s.s., anisakis pegreffii, hysterothylacium aduncum, rhadinorhynchus pristis, mazocraes alosae, hemiurus appendiculatus, ceratothoa italica and an unidentified ergas ... | 2015 | 26141433 |
| anisakis infection in allis shad, alosa alosa (linnaeus, 1758), and twaite shad, alosa fallax (lacépède, 1803), from western iberian peninsula rivers: zoonotic and ecological implications. | spawning individuals of allis shad, alosa alosa (linnaeus, 1758), and twaite shad, alosa fallax (lacépède, 1803), were sampled from three rivers on the atlantic coast of the iberian peninsula (ulla, minho, mondego) during 2008 to 2013 to assess the presence of the zoonotic marine parasite anisakis spp. larvae. the results revealed that both shad species were infected by third-larval stage anisakis simplex s.s. and anisakis pegreffii. the latter is reported in mixed infections in both shad specie ... | 2015 | 25810220 |
| fishing area and fish size as risk factors of anisakis infection in sardines (sardina pilchardus) from iberian waters, southwestern europe. | the sardine (sardina pilchardus) is a fish commonly consumed and appreciated in many countries, although they are more likely to be eaten fresh in western mediterranean countries such as spain, portugal, france or italy. a molecular epidemiological survey of sardines from 5 fishing areas of the spanish mediterranean (málaga, southern spain) and atlantic coasts (southern: cádiz and isla cristina; northern: a coruña and ondarroa) was carried out to determine the presence of anisakis spp. larvae. t ... | 2015 | 25770431 |
| anisakid nematodes as possible markers to trace fish products. | in this work a total of 949 fish samples were analysed for the identification of nematode larvae belonging to the anisakidae family. biomolecular application for the identification of anisakidae larvae can be an optimal instrument for the traceability of fish products, described on the reg. ec 178/2002. results confirm a correlation between geographical distribution of fishes and presence of specific anisakid larvae. fao 37 zone (mediterranean sea) showed a prevailing distribution of anisakis pe ... | 2015 | 27800374 |
| pathogenic potential of two sibling species, anisakis simplex (s.s.) and anisakis pegreffii (nematoda: anisakidae): in vitro and in vivo studies. | the pathogenic potentials of two sibling nematodes anisakis simplex sensu stricto (s.s.) and a. pegreffii were compared by in vitro and in vivo studies. live third-stage larvae of each species were subjected to agar blocks made using pbs or rpmi-1640, overlaid with different supernatants (artificial gastric juice, pbs, and rpmi-1640), and their penetration ability was compared. their tolerance of artificial gastric juice was also tested. further, they were introduced into rats by gastric intubat ... | 2015 | 25685821 |
| haemoglobin, a new major allergen of anisakis simplex. | gastro-allergic anisakiasis and anisakis sensitisation associated chronic urticaria are diseases which differ in their ige and igg4 responses against both crude extract and specific allergens. anisakis and ascaris are closely related nematodes that usually cause problems with specificity in immunodiagnostics. in this study we measured ige and igg4 antibodies against anisakis simplex sensu lato (s. l.) and ascaris suum haemoglobins in sera of 21 gastro-allergic anisakiasis and 23 chronic urticari ... | 2015 | 25683373 |
| survey of anisakis sp. and hysterothylacium sp. in sardines and anchovies from the north adriatic sea. | the occurrence of larval anisakidae and raphidascarididae in anchovies and sardines from the north adriatic sea has been estimated. anisakis pegreffii and hysterothylacium aduncum were reported, with low prevalence values. in brief, a total amount of 7650 fish specimens collected between september 2011 and 2012 were analysed using three different inspection analyses: a visual inspection of the coelomic cavity, an examination of the viscera exploiting the positive hydro-tropism of the larvae (mod ... | 2015 | 25662709 |
| molecular diagnosis of cause of anisakiasis in humans, south korea. | anisakiasis in humans in south korea has been considered to be caused exclusively by the larvae of anisakis simplex sensu stricto and pseudoterranova decipiens. recently, however, dna sequencing of larvae from 15 of 16 anisakiasis patients confirmed the cause to be anisakis pegreffii infection. molecular analysis should be performed for all extracted larvae. | 2015 | 25625427 |
| molecular identification of anisakis and hysterothylacium larvae in marine fishes from the east china sea and the pacific coast of central japan. | anisakiasis is a human disease caused by the accidental ingestion of larvae belonging to the family anisakidae. three fish species, the small yellow croaker pseudosciaena polyactis, the mackerel pneumatophorus japonicus and the hairtail trichiurus haumela are important source for food products in the east china sea. the prevalence and the identification of anisakidae larvae in these fishes will benefit the prevention and control of anisakiasis. in this study, fish samples were obtained from fish ... | 2015 | 25617845 |
| genetic identification and distribution of the parasitic larvae of anisakis pegreffii and anisakis simplex (s. s.) in european hake merluccius merluccius from the tyrrhenian sea and spanish atlantic coast: implications for food safety. | the consumption of the hake merluccius merluccius is widespread in european countries, where this fish has a high commercial value. to date, different larval species of anisakis have been identified as parasites in m. merluccius from european waters, anisakis pegreffii and anisakis simplex (s. s.) being the two most common. the aim of the study is to present data on the occurrence of anisakis spp. larvae in the viscera and flesh of m. merluccius. consequently, the distribution and infection rate ... | 2015 | 25584776 |
| three anisakis spp. isolated from toothed whales stranded along the eastern adriatic sea coast. | knowledge concerning cetacean ecology in the mediterranean is limited but important for sustainable planning and enforcement of appropriate conservation measures. any information that might help to elucidate their ecology is essential. we explored the population and genetic structures of anisakis spp. nematodes isolated from four toothed whale species - bottlenose dolphins (tursiops truncatus), striped dolphins (stenella coeruleoalba), risso's dolphins (grampus griseus) and cuvier's beaked whale ... | 2015 | 25449948 |
| occurrence of anisakid nematode larvae in chub mackerel (scomber japonicus) caught off korea. | chub mackerel (scomber japonicus) is a pelagic fish species widely distributing in the indo-pacific and a commercially important fish species in korea. it is known to harbor anisakid nematodes larvae, and ingesting the raw or undercooked fish can accidentally cause human infection. in this study, we isolated the nematode larvae in 417 chub mackerel caught from 7 sampling locations around the korean peninsula in 2011 and 2012, and identified them by pcr-rflp of the its (internal transcribed space ... | 2014 | 25268324 |
| molecular analysis of anisakis type i larvae in marine fish from three different sea areas in korea. | anisakiasis, a human infection of anisakis l3 larvae, is one of the common foodborne parasitic diseases in korea. studies on the identification of anisakid larvae have been performed in the country, but most of them have been focused on morphological identification of the larvae. in this study, we analyzed the molecular characteristics of 174 anisakis type i larvae collected from 10 species of fish caught in 3 different sea areas in korea. pcr-rflp and sequence analyses of rdna its and mtdna cox ... | 2014 | 25246717 |
| food safety considerations in relation to anisakis pegreffii in anchovies (engraulis encrasicolus) and sardines (sardina pilchardus) fished off the ligurian coast (cinque terre national park, nw mediterranean). | engraulis encrasicolus and sardina pilchardus are pelagic fishes of notable economic and gastronomic importance in the northwest mediterranean (ligurian sea, italy). the consumption of thermally unprocessed or lightly processed, marinated or salted anchovies and sardines presents a potential risk to acquire anisakiasis, a fish-borne parasitic disease in humans. prevalence and abundance of anisakis larvae in engraulis encrasicolus and sardina pilchardus from the monterosso fishing grounds (cinque ... | 2014 | 25203618 |
| anisakidae in fishing products sold in sicily. | one of the parasite diseases associated with the consumption of raw fish that occurs with some frequency is the anisakiasis, a human disease caused by the accidental ingestion of larval nematodes of the genus anisakis, family anisakidae. at the national reference centre for anisakiasis (c.re.n.a.) from october 2012 to february 2013, a number of 231 bony fish (trichiuridae, engraulidae, scombridae and clupeidae) were received from different fishing sites in sicily. anisakis pegreffii is the main ... | 2014 | 27800344 |
| molecular and epidemiological data on anisakis spp. (nematoda: anisakidae) in commercial fish caught off northern sardinia (western mediterranean sea). | anisakiasis is a fish-borne zoonosis caused by third stage larvae of the nematode anisakis sp. present in fish or cephalopods. this is the first contribution to the molecular identification and epidemiology of anisakis spp. in commercial fish from the gulf of asinara (sardinia, western mediterranean sea). between april 2006 to november 2011, 777 specimens of 10 fish species (engraulis encrasicolus, merluccius merluccius, micromesistius poutassou, phycis blennoides, sardina pilchardus, sardinella ... | 2014 | 24630706 |
| molecular identification of anisakis species (nematoda: anisakidae) from marine fishes collected in turkish waters. | anisakid nematodes are important etiological agents for zoonotic human anisakiasis (or anisakidosis). these parasites in the turkish waters still remain unexplored. this study aims the molecular identification of anisakis species in turkey's coast from black, aegean and mediterranean sea and specifically to screen for zoonotic species in commonly commercialized a total of 1145 fish belonging to 31 different species using both polymerase chain reaction-restriction fragment length polymorphism ana ... | 2014 | 24485564 |
| occurrence of hysterothylacium and anisakis nematodes (ascaridida: ascaridoidea) in the tanaka's snailfish liparis tanakae (gilbert & burke) (scorpaeniformes: liparidae). | the tanaka's snailfish liparis tanakae (gilbert & burke) (scorpaeniformes: liparidae) is an economically important marine fish species in china. however, the helminth parasites of this fish are still poorly known. during a helminthological survey of chinese marine fishes from 2011 to 2012, we revealed that l. tanakae was heavily infected with third-stage larvae and adults of ascaridoid nematodes (total prevalence 100% and mean intensity 82.3 nematodes per fish). four species of third-stage larva ... | 2014 | 24464274 |
| ecology and genetic structure of zoonotic anisakis spp. from adriatic commercial fish species. | consumption of raw or thermally inadequately treated fishery products represents a public health risk, with the possibility of propagation of live anisakis larvae, the causative agent of the zoonotic disease anisakidosis, or anisakiasis. we investigated the population dynamics of anisakis spp. in commercially important fish-anchovies (anisakis), sardines (sardina pilchardus), european hake (merluccius merluccius), whiting (merlangius merlangus), chub mackerel (scomber japonicus), and atlantic bl ... | 2014 | 24317085 |
| a cross-reactive monoclonal antibody to nematode haemoglobin enhances protective immune responses to nippostrongylus brasiliensis. | nematode secreted haemoglobins have unusually high affinity for oxygen and possess nitric oxide deoxygenase, and catalase activity thought to be important in protection against host immune responses to infection. in this study, we generated a monoclonal antibody (48eg) against haemoglobin of the nematode anisakis pegreffii, and aimed to characterize cross-reactivity of 4e8g against haemoglobins of different nematodes and its potential to mediate protective immunity against a murine hookworm infe ... | 2013 | 24009787 |
| presence of anisakid larvae in the european anchovy, engraulis encrasicolus, fished off the tyrrhenian coast of central italy. | a survey was carried out to determine the prevalence of anisakid nematode larvae in european anchovy (engraulis encrasicolus) fished off the tyrrhenian coast of central italy. from february through july 2012, 1,490 specimens of e. encrasicolus caught in three different fishing areas (off civitavecchia, anzio, and gaeta in the northern, central, and southern lazio region of italy, respectively) were tested for the presence of anisakid larvae, both by visual microscopic inspection and enzymatic di ... | 2013 | 23992513 |
| experimental susceptibility of gilthead sea bream, sparus aurata, via challenge with anisakis pegreffii larvae. | the endoscopic and histopathological findings detected in sparus aurata experimentally infected with third-stage anisakis larvae without intermediate host are evaluated and discussed. in six fish, live nematode larvae were introduced by gastroscopy into the stomach. the first observation by endoscope, 15 days after challenge, showed the presence of some larvae at the level of gastric mucosa. an explorative celioscopy, performed 60 days after challenge, showed haemorrhages and/or nodules on the g ... | 2013 | 23957000 |
| molecular characterization and comparison of four anisakis allergens between anisakis simplex sensu stricto and anisakis pegreffii from japan. | it remains unclear whether allergens are the same among the sibling species of anisakis simplex sensu lato. this study was carried out to compare the amino acid sequences of three major (ani s 1, 2 and 12) and one minor (ani s 9) anisakis allergens between a. simplex s.s. and anisakis pegreffii. we found 2 (out of 163), 1 (out of 869), and 29 (out of 266) amino acid variable sites for ani s 1, 2, and 12, respectively. however, as both intra- and inter-species variations were present at the same ... | 2013 | 23742817 |
| experimental comparison of pathogenic potential of two sibling species anisakis simplex s.s. and anisakis pegreffii in wistar rat. | there are little data available on the pathology caused by the sibling species anisakis simplex s.s. and anisakis pegreffii. the differences shown in their ability to penetrate the muscle of fish may also be manifested in humans. the purpose of this study is to confirm possible differences in pathogenicity between a. simplex s.s. and a. pegreffii using an experimental model which simulates infection in humans. | 2013 | 23724976 |
| anisakiasis and gastroallergic reactions associated with anisakis pegreffii infection, italy. | human cases of gastric anisakiasis caused by the zoonotic parasite anisakis pegreffii are increasing in italy. the disease is caused by ingestion of larval nematodes in lightly cooked or raw seafood. because symptoms are vague and serodiagnosis is difficult, the disease is often misdiagnosed and cases are understimated. | 2013 | 23621984 |
| survey on the presence of anisakis and hysterothylacium larvae in fishes and squids caught in ligurian sea. | this study aims to investigate the occurrence of anisakidae larvae (genera anisakis and hysterothylacium) in fishes and squids used for human nutrition and increase the knowledge of the distribution of anisakidae larvae in this area of mediterranean sea. distribution and correct identification of anisakidae larvae in fish species is important for the assessment of their relative epidemiological role; especially when the fishes are of high commercial value and largely used in human nutrition. the ... | 2013 | 23537950 |
| epidemiology and molecular identification of anisakis pegreffii (nematoda: anisakidae) in the horse mackerel trachurus trachurus from northern morocco. | anisakis infection parameters were studied in horse mackerel (trachurus trachurus) taken from two areas of northern morocco (tetouan and tangier), which showed a mean prevalence of 54.9%. identification of the its1-5.8s-its2 fragment by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) showed a. pegreffii to be the dominant species; no a. simplex s.s. were detected. the presence of a. pegreffii in horse mackerel was not influenced by the sex (p = 0.46) or catch area ( ... | 2014 | 23461880 |
| molecular characterisation of anisakidae larvae from fish in adriatic sea. | in the present study, anisakids from: tuna (thunnus thynnus) fattened in the croatian farm in middle adriatic sea, three different feral fish species caught near tuna farm (trachurus trachurus, scomber japonicus and oblada melanura) and fish marketed in croatia (t. trachurus) were analysed by morphology and molecular methods. larvae were identified to the species level by pcr restriction fragment length polymorphism and characterised by sequencing of nuclear (internal transcribed spacer) and mit ... | 2012 | 22983167 |
| molecular identification and population dynamic of anisakis pegreffii (nematoda: anisakidae dujardin, 1845) isolated from the european anchovy (engraulis encrasicolus l.) in the adriatic sea. | anchovy engraulis encrasicolus (l.) is a coastal pelagic and euryhaline species that represents the only european species of the family engraulidae, with a widespread distribution. in croatia, it is marketed fresh, frozen, salted or marinated and mainly exported to italy and spain, however anisakis sp. larval infection is frequently the reason for border rejection. since it is known that the prevalence and intensity of anisakis infection varies with fish species, fishing area and season, the aim ... | 2012 | 22647674 |
| anisakis simplex sensu stricto and anisakis pegreffii: biological characteristics and pathogenetic potential in human anisakiasis. | anisakiasis is one of the most common fishborne helminthic diseases in japan, which is contracted by ingesting the larvae of the nematode anisakis spp. carried by marine fish. anisakis simplex sensu stricto (s.s.) and a. pegreffii are the dominant species in fish caught offshore japan. the present study aimed to identify the anisakid species infecting japanese patients and determine whether there is any difference in the pathogenetic potential of a. simplex (s.s.) and a. pegreffii. in total, 41 ... | 2012 | 22545961 |
| molecular identification of anisakis spp. from fishes collected in the tyrrhenian sea (nw mediterranean). | the accurate identification of anisakid nematodes at any life cycle stage is important both to deepen the knowledge on their taxonomy, ecology, epidemiology and for diagnosis and control, as larval stages cause a clinical disease in humans known as anisakidosis. with the aim to investigate the presence of anisakid larvae, specimens of horse mackerel, trachurus trachurus (linnaeus, 1758), silver scabbardfish, lepidopus caudatus (euphrasen, 1788), european anchovy, engraulis encrasicolus (linnaeus ... | 2012 | 22360842 |
| mutation scanning-based analysis of anisakid larvae from sillago flindersi from bass strait, australia. | anisakidosis is an important fish-borne disease caused by the larvae of anisakid nematodes, which affects humans and a range of other animals. the accurate identification of members of this nematode group is central to investigating the epidemiology of the parasites and in the surveillance and control of anisakidosis. it is now well known that morphological identification alone does not allow specific identification, particularly of larval stages. to better understand the epidemiology of anisaki ... | 2012 | 22287178 |
| allergenic activity of pseudoterranova decipiens (nematoda: anisakidae) in balb/c mice. | anisakis simplex is the only fishery-product associated parasite causing clinical allergic responses in humans so far. however, other anisakids, due to the presence of shared or own allergens, could also lead to allergic reactions after sensitization. the aim of this study was to determine if pseudoterranova decipiens belonging to the family anisakidae has allergenic activity and is able to induce sensitization after oral administration in a murine (balb/c mice) model. | 2017 | 28606183 |
| anisakis haemoglobin is a main antigen inducing strong and prolonged immunoreactions in rats. | anisakis simplex larvae are well known to cause gastrointestinal and allergic manifestations after ingestion of parasitized raw or undercooked seafood. the antibody recognition dynamics against the components of anisakis larval antigen after primary and re-infection with anisakis live larvae remain unclear. for this study, immunoblot analyses of serum igg, ige, and igm against anisakis larval somatic extract were performed in rats that had been orally inoculated with a. simplex live larvae. mult ... | 2017 | 28497226 |
| occurrence of anisakis (nematoda: anisakidae) larvae in unusual hosts in southern hemisphere. | nematodes belonging to the genus anisakis are important parasites due to their abundance in seafood and health impacts on humans. in the present study anisakis larvae were found in a number of uncommon hosts including the grey petrel, procellaria cinerea, the little penguin, eudyptula minor, blue-lipped sea krait, laticauda laticaudata and spinner shark, carcharhinus brevipinna. morphological examination showed nematodes in these animals are anisakis larval type i. genetic characterisation sugge ... | 2017 | 28797592 |
| parasitic infections in the east asian finless porpoise neophocaena asiaeorientalis sunameri living off the chinese yellow/bohai sea coast. | parasitic disease is among the major causes of health problems in marine mammals. however, little information on parasitic species composition and infection levels in finless porpoises (neophocaena spp.) is available. in this study, we report the first systematic survey on parasitic infections in the east asian finless porpoise n. asiaeorientalis sunameri (eafp) living off the chinese yellow/bohai sea coast. using both morphological and molecular methods, 5 parasitic helminths were identified: c ... | 2017 | 28627493 |
| invasive anisakiasis by the parasite anisakis pegreffii (nematoda: anisakidae): diagnosis by real-time pcr hydrolysis probe system and immunoblotting assay. | anisakiasis is a fish-borne zoonosis caused by anisakis spp. larvae. one challenging issue in the diagnosis of anisakiasis is the molecular detection of the etiological agent even at very low quantity, such as in gastric or intestinal biopsy and granulomas. aims of this study were: 1) to identify three new cases of invasive anisakiasis, by a species-specific real-time pcr probe assay; 2) to detect immune response of the patients against the pathogen. | 2017 | 28764637 |
| ige sensitization to anisakis pegreffii in italy: comparison of two methods for the diagnosis of allergic anisakiasis. | ige sensitization to anisakis pegreffii in italian subjects suffering from gastro-allergic anisakiasis (gaa) (n=5), or showing chronic urticaria (cu+) after fish consumption (n=100), was investigated. a control group (n=5) was also included. ige response was analysed by immunoblotting (wb) assay, using both excretory/secretory products (esps) and crude extract (ce) of a. pegreffii larvae. the results were compared with those achieved by the conventional immunological method for anisakis allergy ... | 2017 | 28475216 |
| distribution of anisakid nematodes parasitizing rajiform skates under commercial exploitation in the southwestern atlantic. | in order to evaluate the infestation by anisakids present in elasmobranchs and their distribution in the argentine sea, this study was carried at a regional scale with the following aims: 1) to identify those anisakid species present in skates under exploitation; 2) to characterize quantitatively these infestations and 3) to determine those factors driving the variability in parasite burdens across skate species. a total of 351 skates, belonging to 3 species (218 sympterygia bonapartii, 86 zeara ... | 2018 | 29277002 |
| anisakis spp. larvae in three mesopelagic and bathypelagic fish species of the central mediterranean sea. | in this work 437 fish samples of species belonging to the families myctophidae (electrona risso and diaphus metopoclampus) and phosichthyidae (vinciguerria attenuata) were examined for the presence of anisakidae larvae. the study was performed with fishes in the central mediterranean sea, particularly in the strait of sicily and in the strait of messina. the visual inspection and chloro-peptic analysis revealed the presence of nematode parasites with prevalence values between 2.9% in electrona r ... | 2018 | 28965943 |
| tissue-specific transcriptomes of anisakis simplex (sensu stricto) and anisakis pegreffii reveal potential molecular mechanisms involved in pathogenicity. | larval stages of the sibling species of parasitic nematodes anisakis simplex (sensu stricto) (s.s.) (as) and anisakis pegreffii (ap) are responsible for a fish-borne zoonosis, known as anisakiasis, that humans aquire via the ingestion of raw or undercooked infected fish or fish-based products. these two species differ in geographical distribution, genetic background and peculiar traits involved in pathogenicity. however, thus far little is known of key molecules potentially involved in host-para ... | 2018 | 29321072 |
| anisakis spp. larvae in different kinds of ready to eat products made of anchovies (engraulis encrasicolus) sold in italian supermarkets. | in this study the occurrence of visible anisakid larvae in semi-preserved anchovy products sold on the italian market was investigated. totally, 107 ready to eat products (33 salted-ripened, 49 in oil and 25 marinated) were sampled. each sample was digested, then the digested material was observed under natural and uv light. parasites were counted, collected and microscopically identified to genus level. a representative subset was molecularly identified using the cox2 gene. at least one visible ... | 2018 | 29306733 |
| geographic and host size variations as indicators of anisakis pegreffii infection in european pilchard (sardina pilchardus) from the mediterranean sea: food safety implications. | european pilchards are traditionally eaten marinated or salted in the mediterranean countries often without thermal processing or gutting due to small size. since ingestion of live third stage anisakis larvae represents a causing agent in the onset of anisakiasis, the aim of our study was to assess prevalence and intensity of anisakis infection in european pilchards originating from different mediterranean regions in a three-year sampling period (2013-2015). a total of 1564 specimens of european ... | 2017 | 29216552 |
| excretory/secretory proteases and mechanical movement of anisakis pegreffii infective larvae in the penetration of balb/c mice gastrointestine. | anisakiasis is a human parasitic disease caused by infection with the infective larvae of anisakis. accidental infection in humans causes the gastrointestinal pathophysiological effects of mechanical tissue damage by migrating larvae. the mechanism of the infective larval invasion and migration is suspected to involve larval excretory/secretory proteases and motility. this study demonstrates the penetration rate of the infective larvae of anisakis pegreffii in mouse gastrointestine depends on th ... | 2017 | 29132548 |
| molecular and immunological characterisation of tropomyosin from anisakis pegreffii. | tropomyosin (tm) is a major allergen in shellfish, known to cross-react with mite, cockroach and/or some roundworm (nematode) tm. in this study, we aimed to express and purify tm from the parasitic nematode anisakis pegreffii and also to characterise its cross-reactivity with tm from shellfish. a. pegreffii was isolated from the flathead tiger fish (neoplatycephalus richardsoni) and characterised using single-strand conformation polymorphism (sscp)-based sequencing of the first and second intern ... | 2017 | 29038898 |