Publications

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morphological and molecular characterization of anisakis larvae (nematoda: anisakidae) in beryx splendens from japanese waters.the third-stage (l3) larvae of anisakis, which are the etiological agents of human anisakiasis, have been categorized morphologically into anisakis type i larvae and anisakis type ii larvae. genetic analysis has allowed easy identification of these larvae: anisakis type i larvae include the species anisakis simplex sensu stricto, anisakis pegreffii, anisakis simplex c, anisakis typica, anisakis ziphidarum, and anisakis nascettii, whereas anisakis type ii larvae include the species anisakis physe ...201121377540
integrative taxonomy of anisakid nematodes in stranded cetaceans from brazilian waters: an update on parasite's hosts and geographical records.anisakidae are represented mainly by species of anisakis dujardin, 1845, pseudoterranova krabbe, 1878, and contracaecum railliet and henry, 1913. integrative taxonomy, based on morphological and genetic approaches, can provide a precise identification of species, increasing the knowledge of their systematics, biology, and evolution. in this study, integrative taxonomy was applied in order to identify, at generic and/or specific taxonomic level, 245 anisakids from 63 cetaceans of 12 different spe ...201728975458
metazoan parasite infection in the swordfish, xiphias gladius, from the mediterranean sea and comparison with atlantic populations: implications for its stock characterization.thirteen parasite taxa were identified in the mediterranean swordfish by morphological and genetic/molecular methods. the comparison of the identified parasite taxa and parasitic infection values observed in the mediterranean swordfish showed statistically significant differences with respect to those reported for its atlantic populations. a stepwise linear discriminant analysis of the individual fish examined showed a separation among three groups: one including fish from the mediterranean sea ...201425057787
effect of different temperatures on the expression of the newly characterized heat shock protein 90 (hsp90) in l3 of anisakis spp. isolated from scomber australasicus.anisakid nematodes are distributed worldwide in a wide variety of marine fishes and they are known to cause the zoonotic disease, anisakiasis. the temperature control is commonly applied for prevention and control of anisakiasis. to analyze the cellular response to temperature stress in anisakis, the heat shock protein 90 (hsp90) was chosen in the present study, as it plays a key role in many cellular processes and responds to stress conditions such as heat or cold shock. anisakids were sampled ...201425269988
occurrence and prevalence of fish-borne anisakis larvae in the spotted mackerel scomber australasicus from taiwanese waters.anisakid nematodes have been found in a variety of marine fishes throughout the world and they are known to cause anisakiasis in human hosts. the present study investigated the prevalence of potentially zoonotic anisakid larvae in spotted mackerel caught from taiwanese waters where fish represents an important food sources. anisakis third-stage larvae (l3, n=502) were isolated from 250 spotted mackerel scomber australasicus. anisakis l3 larvae were divided morphologically into two types, anisaki ...201525733490
scavenging hagfish as a transport host of anisakid nematodes.hagfish are the most primitive craniates and scavengers, feeding on dead organisms as well as fish and invertebrates. hagfish play an important ecological role in recycling nutrients, helping to recycle biomass from the upper water column. we investigated 265 specimens of four hagfish species, including eptatretus burgeri, eptatretus yangi, eptatretus sheni and eptatretus taiwanae from northeastern taiwanese waters of the northwestern pacific from november 2013 to june 2014. eight species of ani ...201626872923
molecular characterization and phylogeny of anisakid nematodes from cetaceans from southeastern atlantic coasts of usa, gulf of mexico, and caribbean sea.in the present study, 407 anisakid nematodes, collected from 11 different species of cetaceans of the families delphinidae, kogiidae, physeteridae, and ziphiidae, from the southeastern atlantic coasts of usa, the gulf of mexico, and the caribbean sea, were examined morphologically and genetically characterized by pcr restriction fragment length polymorphism to identify them to species level, assess their relative frequencies in definitive hosts, and determine any host preference. sequence data f ...201121212982
additional records of metazoan parasites from caribbean marine mammals, including genetically identified anisakid nematodes.studies of marine mammal parasites in the caribbean are scarce. an assessment for marine mammal endo- and ectoparasites from puerto rico and the virgin islands, but extending to other areas of the caribbean, was conducted between 1989 and 1994. the present study complements the latter and enhances identification of anisakid nematodes using molecular markers. parasites were collected from 59 carcasses of stranded cetaceans and manatees from 1994 to 2006, including globicephala macrorhynchus, kogi ...200919582477
anisakid parasites of fishes caught on the african shelf.anisakids of scomber japonicus, trachyrincus scabrus, sardina pilchardus, merluccius polli, trachurus trachurus, and hoplostethus cadenati from african shelf are reported. parasites were extensively studied with respect to their morphology and sequence analysis of the rdna fragment (containing internal transcribed spacer [its]-1, 5.8s subunit and its-2); all of them are approximately 1,000 base pairs. digestion of the amplified region and its sequencing revealed the presence of a sibling species ...200919093709
genetic divergence and reproductive isolation between anisakis brevispiculata and anisakis physeteris (nematoda: anisakidae)s.in order to assess the taxonomic status of anisakis brevispiculata dollfus, 1966 population samples of this taxon from central and south-eastern atlantic ocean were compared at 22 enzymatic loci with samples belonging to anisakis physeteris baylis, 1923 from the mediterranean sea and central-eastern atlantic ocean. very low interpopulational genetic divergence was observed both within a. brevispiculata (average d(nei) = 0.008) and within a. physeteris (d(nei) = 0.009) despite the geographic dist ...200111165265
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