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phytochemical, pharmacological, antibacterial and anti-ovulatory studies on annona squamosa. 19751178793
bullatacin, bullatacinone, and squamone, a new bioactive acetogenin, from the bark of annona squamosa.activity-directed fractionation of the stem bark of annona squamosa, monitoring with brine shrimp lethality, led to the isolation of the highly bioactive acetogenins bullatacin [1] and bullatacinone [2], thus demonstrating a new abundant plant source for these potent compounds. a new keto-monotetrahydrofuran acetogenin with a ketolactone terminus, as first seen in bullatacinone [2], was also isolated, characterized by spectral analyses, and named squamone [3]. the cytotoxicities of 3 were increa ...19902348205
larvicidal potential of five philippine plants against aedes aegypti (linnaeus) and culex quinquefasciatus (say).five species of philippine plants, reported in the literature to have insecticidal properties, were selected for investigation, namely: anona squamosa ("atis" or sugar apple), eucalyptus globulus ("bagras" or olive gum eucalyptus), lansium domesticum ("lansones"), azadirachta indica ("neem") and codiaeum variegatum ("san francisco" or croton). these were screened and assayed for their larvicidal potential against aedes aedes aegypti (linnaeus) and culex quinquefasciatus (say) by exposing 3rd-4th ...19947667727
identification of ent-16 beta, 17-dihydroxykauran-19-oic acid as an anti-hiv principle and isolation of the new diterpenoids annosquamosins a and b from annona squamosa.phytochemical analysis of the fruits of annona squamosa yielded 12 known kaurane derivatives (1-11, 13) and two new kaurane diterpenoids, which have been named annosquamosin a (16 beta-hydroxy-17-acetoxy-ent-kauran-19-al) (12) and annosquamosin b (19-nor-ent-kaurane-4 alpha,16 beta,-17-triol) (14). the structures of the new compounds were established by spectral analyses and chemical evidence. among these 14 compounds, 16 beta, 17-dihydroxy-ent-kauran-19-oic acid (2) showed significant activity ...19968786370
squamotacin: an annonaceous acetogenin with cytotoxic selectivity for the human prostate tumor cell line (pc-3).the bark extracts of annona squamosa yielded a new bioactive acetogenin, squamotacin (1), and the known compound, molvizarin (2), which is new to this species. compound 1 is identical to the potent acetogenin, bullatacin (3), except that the adjacent bistetrahydrofuran (thf) rings and their flanking hydroxyls are shifted two carbons toward the gamma-lactone ring. compound 1 showed cytotoxic selectively for the human prostate tumor cell line (pc-3), with a potency of over 100 million times that o ...19968991957
novel mono-tetrahydrofuran ring acetogenins, from the bark of annona squamosa, showing cytotoxic selectivities for the human pancreatic carcinoma cell line, paca-2.the bark of annona squamosa yielded three new mono-tetrahydrofuran (thf) ring acetogenins, each bearing two flanking hydroxyls and a carbonyl group at the c-9 position. these compounds were isolated using the brine shrimp lethality assay as a guide for the bioactivity-directed fractionation. (2,4-cis and trans)-mosinone a (1) is a mixture of ketolactone compounds bearing a threo/trans/threo ring relationship and s double bond two methylene units away from the flanking hydroxyl. the other two new ...19979214729
modifications of allergenicity linked to food technologies.the prevalence of food allergies (fa) has increased over the past fifteen years. the reasons suggested are changes in dietary behaviour and the evolution of food technologies. new cases of fa have been described with chayote, rambutan, arguta, pumpkin seeds, custard apple, and with mycoproteins from fusarium.... additives using food proteins are at high risk: caseinates, lysozyme, cochineal red, papaïn, alpha-amylase, lactase etc. heating can reduce allergenicity or create neo-allergens, as well ...19989503097
mono-thf ring annonaceous acetogenins from annona squamosa.continuing work on the bark of annona squamosa rich. (annonaceae), directed by the brine shrimp lethality test (bst), has resulted in the isolation of three new annonaceous acetogenins, 4-deoxyannoreticuin, cis-4-deoxyannoreticuin, and (2,4-cis and trans)-squamoxinone. the first two are additional examples of acetogenins isolated from this plant species which contain the unusual feature of an oxygen functionality at the c-9 position. they have a hydroxylated mono-thf ring with respective threo/t ...19989542173
antiplasmodial activity of selected sudanese medicinal plants with emphasis on maytenus senegalensis (lam.) exell.the antiplasmodial activity of plant extracts related to four families was tested on chloroquine sensitive strain 3d7 and chloroquine resistant strain dd2 of plasmodium falciparum. the methanolic extract of harrisonia abyssinica (simaroubaceae) inhibited dd2 with ic50 value of 4.7 microg/ml, while in 3d7, the ic50 value was 10 microg/ml. most of the plants from the family meliaceae showed highly potent antiplasmodial activity against the two tested strains. khaya senegalensis, azadirachta indica ...199910363837
possible relation of atypical parkinsonism in the french west indies with consumption of tropical plants: a case-control study. caribbean parkinsonism study group.in europe and north america, parkinson's disease is the major form of parkinsonism; less than 4% of cases are progressive supranuclear palsy (psp) and about 20% are atypical parkinsonism. the distribution of these subgroups is different in the french west indies. we aimed to define the clinical and demographic specificity of these disorders in guadeloupe and to investigate a postulated link with consumption of herbal tea and fruits from the annonaceae family (annona muricata and annona squamosa) ...199910440304
preliminary studies on activity of ocimum sanctum, drynaria quercifolia, and annona squamosa against neisseria gonorrhoeae.despite the progressive increase of antimicrobial resistance of neisseria gonorrhoeae worldwide, there are limited reports of alternative remedies from plants.200515668617
hypoglycemic and antidiabetic effect of ethanolic extract of leaves of annona squamosa l. in experimental animals.the ethanolic extract of annona squamosa l. (annonaceae) leaves was administered orally at different doses to normal as well as streptozotocin (stz)-induced diabetic rats and alloxan-induced diabetic rabbits. the dose of 350 mg/kg body weight (bw) reduced the fasting blood glucose (fbg) level by 6.0% within 1 h, whereas, the peak blood glucose at 1 h during glucose tolerance test (gtt) was reduced by 17.1% in normal rats. the same dose of ethanolic extract reduced fbg by 26.8% and improved gluco ...200515848023
major volatile constituents of annona squamosa l. bark.the volatile constituents of annona squamosa l. bark were identified from the essential oil obtained by steam distillation and studied by gc/ms. six major components were identified as 1h-cycloprop(e)azulene (3.46%), germacrene d (11.44%), bisabolene (4.48%), caryophyllene oxide (29.38%), bisabolene epoxide (3.64%) and kaur-16-ene (19.13%). the oil was also screened for its antimicrobial activity, which exhibited a significant antimicrobial activity against bacillus subtilis and staphylococcus a ...200616753909
ethnomedicines used in trinidad and tobago for urinary problems and diabetes mellitus.this paper is based on ethnobotanical interviews conducted from 1996-2000 in trinidad and tobago with thirty male and female respondents.200617040567
anti-head lice effect of annona squamosa seeds.the present study focused on the separation and identification of the active compounds against head lice from the hexane extract of annona squamosa l seed. chromatographic and spectroscopic techniques revealed that two major compounds of the hexane seed extract were oleic acid and triglyceride with one oleate ester. the yields of these compounds were 13.25% and 7.74% dry weight, respectively. the compounds were tested in vitro against head lice, comparing to the crude hexane extract of the seed. ...200617120975
larvicidal and adulticidal activities of some medicinal plants against the malarial vector, anopheles stephensi (liston).the present study was undertaken to test the efficacy of 11 commonly available medicinal plants and compare its efficacy in relation to larvicidal and mosquitocidal activities against larvae and adults of anopheles stephensi (liston). all the medicinal plants and the mixture were effective against larvae of a. stephensi as evidenced by low lethal concentration and lethal time. the lethality varied in adults and plant extracts of mixture; eucalyptus globulus, cymbopogan citratus, artemisia annua, ...200918787842
diversity of root associated microorganisms of selected medicinal plants and influence of rhizomicroorganisms on the antimicrobial property of coriandrum sativum.the total heterotrophic bacteria, actinomycetes and fungus were enumerated from the rhizosphere and non-rhizosphere soil of 50 selected locally available medicinal plants in and around bharathiar university. in all the plants, population of microorganism were higher in the rhizosphere soil than in the non rhizosphere soil. among the microorganisms, bacterial population was higher in number followed by fungus and actinomycetes. of the medicinal plants, the maximum rhizosphere effect was observed ...200818831345
beneficial effects of annona squamosa extract in streptozotocin-induced diabetic rats.the present study investigated the possible therapeutic effects of annona squamosa (a. squamosa) extract on certain biochemical markers in streptozotocin (stz)-induced diabetes mellitus in rats.200818946614
in vivo assessment of genotoxic effects of annona squamosa seed extract in rats.widespread use of pesticides represents a potential risk to human and environmental health. hence, biopesticides from plants are some of the future strategies for plant protection. in this regard, a seed extract of annona squamosa was prepared and found to be a promising pesticide. in order to establish the inherent toxicity and non-target safety required for registration and marketing of pesticides, toxicological studies are conducted. the genotoxicity potential was evaluated in rats with 75, 1 ...200919457440
larvicidal and igr activity of extract of tanzanian plants against malaria vector mosquitoes.this paper reports the larvicidal activity of seventeen tanzanian plant species against the malaria vector, anopheles gambiae s.s. giles larvae. some of the plants are used traditionally as sources of insecticidal materials.200919502695
anthelmintic acetogenin from annona squamosa l. seeds.annona squamosa seeds extracts showed anthelmintic activity against haemonchus contortus, the main nematode of sheep and goat in northeastern brazil. a compound 1 was isolated from ethyl acetate extract and inhibited the egg hatching of h. contortus at 25 mg ml(-1). the structure of 1 was determined as a c37 trihydroxy adjacent bistetrahydrofuran acetogenin based on spectroscopic analysis.200818506253
analgesic and anti-inflammatory activity of caryophyllene oxide from annona squamosa l. bark.caryophyllene oxide was isolated from an unsaponified petroleum ether extract of the bark of annona squamosa and studied for its analgesic and anti-inflammatory activity. caryophyllene oxide at the doses of 12.5 and 25mg/kg body wt. and unsaponified petroleum ether extract at a dose of 50mg/kg body wt. showed significant central as well as peripheral analgesic, along with anti-inflammatory, activity. these activities of caryophyllene oxide were comparable with the standard drug used in the respe ...201019576741
in vivo evaluation of anti-oxidant and anti-lipidimic potential of annona squamosa aqueous extract in type 2 diabetic models.diabetes is known to involve oxidative stress and changes in lipid metabolism. many secondary plant metabolites have been shown to possess antioxidant activities, improving the effects of oxidative stress due to diabetes. the present study was aimed to evaluate the effect of water extract of annona squamosa leaves on antioxidant enzymes and lipid profile of animal models of type 2, non-insulin dependent diabetes mellitus (niddm).200818440739
adulticidal and larvicidal efficacy of some medicinal plant extracts against tick, fluke and mosquitoes.the adulticidal and larvicidal effect of indigenous plant extracts were investigated against the adult cattle tick haemaphysalis bispinosa neumann, 1897 (acarina: ixodidae), sheep fluke paramphistomum cervi zeder, 1790 (digenea: paramphistomatidae), fourth instar larvae of malaria vector, anopheles subpictus grassi and japanese encephalitis vector, culex tritaeniorhynchus giles (diptera: culicidae). the aim of this study was to evaluate the toxic effect of leaf hexane, chloroform, ethyl acetate, ...200919819626
protective role of annona squamosa linn bark extracts in dmba induced genotoxicity.there has been no dearth of scientific literature that genotoxic agents have a causative effect in the pathogenesis of carcinomas. 7,12 dimethylbenz(a)anthracene (dmba), which is a potent organ specific carcinogen, has been found to produce mutagenic effects on cells. annona squamosa has a long history in traditional indian medicine for being used to treat several diseases including cancer.200820071820
antioxidant activities of some local bangladeshi fruits (artocarpus heterophyllus, annona squamosa, terminalia bellirica, syzygium samarangense, averrhoa carambola and olea europa).in the present study, antioxidant activities of the fruits of a. heterophyllus, a. squamosa, t. bellirica, s. samarangense, a. carambola and o. europa were investigated. for this, at first matured fruits of them were sliced into small pieces and dried in the sun and finally crushed in a grinder to make powder. ethanolic extracts of fruit powder were prepared using 99.99% ethanol. the antioxidative activities of these extracts were determined according to their abilities of scavenging 1, 1-diphen ...200717460898
analgesic and anti-inflammatory activities of 18-acetoxy-ent-kaur-16-ene from annona squamosa l. bark.the annona squamosa l. bark was collected from ahmednagar district, india. 18-acetoxy-ent-kaur-16-ene was isolated from petroleum ether extract (pe) and studied for its analgesic and anti-inflammatory activities. 18-acetoxy-ent-kaur-16-ene at the doses of 12.5 and 25 mg/kg, and pe at a dose of 50 mg/kg exhibited significant analgesic along with anti-inflammatory activity.201020957519
vasorelaxant activity of cyclic peptide, cyclosquamosin b, from annona squamosa.a cyclic octapeptide, cyclosquamosin b (2), isolated from the seeds of annona squamosa showed a vasorelaxant effect on rat aorta. it showed a slow relaxation activity against norepinephrine (ne)-induced contractions of rat aorta with/without endothelium. it showed inhibition effect on vasocontraction of depolarized aorta with high concentration potassium, but moderately inhibition effect on ne-induced contraction in the presence of nicardipine. these results showed that the vasorelaxant effect b ...200616784851
efficacy of anthelmintic properties of medicinal plant extracts against haemonchus contortus.the development of anthelmintic resistance has made the search for alternatives to control gastrointestinal nematodes of small ruminants imperative. among these alternatives are several medicinal plants traditionally used as anthelmintics. this present work evaluated the efficacy of ethyl acetate, acetone, and methanol dried leaf and seed extracts of five medicinal plants were tested in vitro ovicidal and larvicidal activities on haemonchus contortus. the in vitro assay was based on egg hatch as ...201020980034
examination of some morphologically unusual cultures of phytophthora species using a mitochondrial dna miniprep technique and a standardised sporangium caducity assessment.using the mitochondrial dna miniprep technique, the identity of sixteen morphologically unusual cultures allocated to phytophthora nicotianae, phytophthora mexicana or phytophthora porri was determined by comparison with a library of mtdna band patterns obtained from reference cultures. seven cultures were identified as phytophthora nicotianae (including those assigned to phytophthora mexicana and phytophthora porri), six as strains of phytophthora palmivora with small, ovoid, weakly caducous sp ...199716284813
nutritional and hypoglycemic effect of fruit pulp of annona squamosa in normal healthy and alloxan-induced diabetic rabbits.the nutritive value of the pulp of the edible fruit of annona squamosa and its effect on various biochemical parameters has been assessed in normal and alloxan-induced diabetic rats. different doses (2.5, 5.0, 10.0 g/kg b.w.) of fresh fruit pulp of a. squamosa were given to the three groups each of normal healthy and diabetic rabbits orally daily for 1 month. there was a control group of normal as well as diabetic animals which did not receive fruit pulp. protein efficiency ratio (per), digestib ...200516230844
differential cytotoxic effects of annona squamosa seed extracts on human tumour cell lines: role of reactive oxygen species and glutathione.annonaceous acetogenins are a new class of compounds that have been reported to have potent pesticidal, parasiticidal, anti-microbial, cell growth inhibitory activities. in this study, organic and aqueous extracts from the defatted seeds of annona squamosa (custard apple) were tested on different human tumour cell lines for antitumoural activity. while organic and aqueous extracts induced apoptosis in mcf-7 and k-562 cells, they failed to do so in colo-205 cells. treatment of mcf-7 and k-562 cel ...200515886460
toxicity of botanical insecticides on golden apple snail (pomacea canaliculata).the molluscicidal activity of crude extracts from five highly potential plants, annona squamosa seed, nerium indicum leaves, stemona tuberose root, cyperus rotundus corm and derris elliptica root was assessed to pomacea canaliculata. d. elliptica root and c. rotundus corm extracts showed the highest toxicity against 3-month old snails which have lc50 as 23.68 +/- 2.96 mg/l and 133.20 +/- 7.94 mg/l, respectively. the c. rotundus corm extracts were chosen for detoxification enzyme in vivo assay wh ...201021542482
antiplasmodial potential of medicinal plant extracts from malaiyur and javadhu hills of south india.the emergence and spread of plasmodium falciparum with resistance to chloroquine (cq), the safest and cheapest anti-malarial drug, coupled with the increasing cost of alternative drugs especially in developing countries have necessitated the urgent need to tap the potential of plants for novel anti-malarials. the present study investigates the anti-malarial activity of the methanolic extracts of 13 medicinal plants from the malaiyur and javadhu hills of south india against blood stage cq-sensiti ...201121643655
larvicidal activity of medicinal plant extracts against anopheles subpictus & culex tritaeniorhynchus.background & objectives: mosquitoes transmit serious human diseases, causing millions of deaths every year and the development of resistance to chemical insecticides resulting in rebounding vectorial capacity. plants may be alternative sources of mosquito control agents. the present study assessed the role of larvicidal activities of hexane, chloroform, ethyl acetate, acetone, and methanol dried leaf and bark extracts of annona squamosa l., chrysanthemum indicum l., and tridax procumbens l. agai ...201121808141
Acaricidal, insecticidal, and larvicidal efficacy of fruit peel aqueous extract of Annona squamosa and its compounds against blood-feeding parasites.Plant products may be alternative sources of parasitic control agents, since they constitute a rich source of bioactive compounds that are eco-friendly and nontoxic products. The plant extracts are good and safe alternatives due to their low toxicity to mammals and easy biodegradability. In the present study, fruit peel aqueous extract of Annona squamosa (Annonaceae) extracted by immersion method exhibited adulticidal activity against Haemaphysalis bispinosa (Acarina: Ixodidae) and the hematopha ...201122006187
evaluation of anthelmintic properties of some plants used as livestock dewormers against haemonchus contortus infections in sheep.gastrointestinal helminth infections remain a major constraint to livestock production globally. this study evaluated anthelmintic efficacy of 7 plants used as dewormers by farmers and pastoralists in kenya. thus 3 commercial anthelmintics and 7 plant preparations were tested in lambs infected with 5000 or 3000 l3 haemonchus contortus in 4 experiments. in the first experiment, ivermectin, levamisole and albendazole were tested in 46 lambs. seven plant preparations of hagenia abyssinica, olea eur ...200415376783
Contraceptive efficacy and safety of HerbOshield™ vaginal gel in rats.Spermicides represent one of the methods of contraception. The synthetic agents available as spermicides produce severe side effects. Hence, there is a need to replace these agents with safe and effective agents such as plant-based contraceptive agents.201222067802
aqueous extract of annona squamosa (l.) ameliorates renal failure induced by 5/6 nephrectomy in rat.to assess the renoprotective activity of the water extract of annona squamosa in 5/6 nephrectomized animals.201122144782
effect of herbal molluscicides and their combinations on the reproduction of the snail lymnaea acuminata.effects of sublethal treatment (20 and 60% of lc50/24 h) of the plant-derived molluscicides annona squamosa linn. and lawsonia inermis linn. and their combinations with other herbal molluscicides, such as cedrus deodara roxb, azadirachta indica a. juss, bulb powder of allium sativum linn. and polianthes tuberosa linn., and oleoresin of zingiber officinale rosc., and acetogenins extracted from the seeds of a. squamosa linn., on the reproduction of the snail lymnaea acuminata have been studied. it ...200415253044
antidiabetic activity of aqueous leaf extract of annona squamosa in streptozotocin-nicotinamide type 2 diabetic rats.the claim by some tribal populations in parts of northern india that the young leaves of the custard apple tree, annona squamosa l. (annonaceae) has antidiabetic properties was investigated. diabetes mellitus was induced with streptozotocin-nicotinamide and graded doses of the aqueous leaf extracts were then administered in drinking water to normal and experimental diabetic rats for 12 days. fasting plasma glucose levels, serum insulin levels, serum lipid profiles and changes in body weight were ...200415036485
antibacterial activity of three medicinal thai plants against campylobacter jejuni and other foodborne pathogens.leaves of adenanthera pavonina, moringa oleifera and annona squamosa are used in traditional thai medicine to treat dysentery and other diseases. this study investigated the antibacterial activity of these plants against six species of foodborne pathogen. methods and solvents employed to extract active constituents were optimised using the disc diffusion assay. phytochemical analysis of the optimised extracts was performed by thin layer chromatography (tlc). minimum inhibitory concentrations (mi ...201121878033
estimation of phytochemicals and antioxidant activity of underutilized fruits of andaman islands (india).the present study aimed to determine the antioxidant activity and phytochemical contents in 10 underutilized fruits of andaman islands (india) namely malpighia glabra l., mangifera andamanica l., morinda citrifolia l., syzygium aqueum (burm.f) alst., annona squamosa l., averrhoa carambola l., averrhoa bilimbi l., dillenia indica l., annona muricata l. and ficus racemosa l. the antioxidant activity varied from 74.27% to 98.77%, and the methanol extract of m. glabra showed the highest antioxidant ...201122080844
effects of annona squamosa extract on early pregnancy in rats.annona squamosa linn., family annonaceae, is said to show varied medicinal effects, including insecticide, antiovulatory and abortifacient. the purpose of present study was to investigate if a. squamosa seed aqueous extract, in doses higher than that popularly used to provoke abortion, interferes with reproductive performance, and to correlate the ingestion of this extract with possible alterations in rat embryonic implantation. doses of 300 mg/kg (treated group i, n = 17) and 600 mg/kg (treated ...200212487333
new ent-kaurane diterpenoids with anti-platelet aggregation activity from annona squamosa.a phytochemical investigation on the stems of annona squamosa led to the isolation of six new ent-kaurane diterpenoids, annomosin a (16beta-hydroxy-19-al-ent-kauran-17-yl 16beta-hydro-19-al-ent-kauran-17-oate) (1), annosquamosin c (16alpha-hydro-17-hydroxy-19-nor-ent-kauran-4alpha-ol) (2), annosquamosin d (16beta-acetoxy-17-hydroxy-19-nor-ent-kauran-4alpha-ol) (3), annosquamosin e (16beta-hydroxy-17-acetoxy-19-nor-ent-kauran-4alpha-formate) (4), annosquamosin f (16beta-hydroxy-17-acetoxy-18-nor- ...200212398544
molluscicidal properties of some species of annona.in search for plant molluscicides for the vector control of schistosomiasis, ethanolic extracts from different parts of six species of the annonaceae family were evaluated against adult forms and egg masses of biomphalaria glabrata. results from accurate experiments indicate that the majority of analyzed extracts possess properties lethal to biomphalaria glabrata, some of them with significant ld90 values (< 20 ppm), as showed for annona crassiflora m: [pulp and seed (ld50 = 13.21), steem (2.34) ...200111315753
an actidione resistant candida tropicalis from custard apple juice.an actidione resistant yeast, candida tropicalis, was isolated from fermenting custard apple juice. though a slight inhibition of growth was observed on the first day with 5000 ppm of actidione, growth was equal to control after one week. sorbic acid at 500 ppm and above inhibited the growth of this yeast while sodium benzoate and potassium metabisulphite were unable to suppress the growth even at 1000 ppm. fermentation and assimilation of different carbon sources were delayed in the presence of ...19817259152
phenolic compounds and in vitro antibacterial and antioxidant activities of three tropic fruits: persimmon, guava, and sweetsop.in our previous study, we have found that persimmon, guava, and sweetsop owned considerably high antioxidant activity and contained high total phenolic contents as well. in order to further supply information on the antibacterial and antioxidant activity of these three tropic fruits, they were extracted by 80% methanol. we then examined the extractions about their phenolic compounds and also studied the extractions and phenolic contents about their minimum inhibitory concentration (mic) and mini ...201627648444
larvicidal and cytotoxic potential of squamocin on the midgut of aedes aegypti (diptera: culicidae).acetogenins are secondary metabolites exclusively produced by annonaceae, which have antitumor, cytotoxic, and pesticide activities. in this study, we evaluated the larvicidal and cytotoxic effect of squamocin from annona squamosa on aedes aegypti (diptera: culicidae) midgut. the compound was solubilized in 2% tween 20 at 10, 20, 50, 80 and 100 ppm. the assay was conducted in a completely randomized design with four replications, each with 20 third-instar larvae. larval mortality was assessed ev ...201424674934
potential applications for annona squamosa leaf extract in the treatment and prevention of foodborne bacterial disease.foodborne disease is a major public health problem. the present study examined annona squamosa leaves, which are traditionally used to treat diarrhea and other infections, for their potential to be used in modern food safety or medicine. active constituents were partially purified by ethanol extraction and column chromatography. mics of the extract were 62.5 to 125 microg/ml against bacillus cereus, listeria monocytogenes and staphylococcus aureus, and 250 microg/ml against campylobacter jejuni. ...201323678817
post-coital antifertility activity of annona squamosa and ipomoea fistulosa. 1979432307
tertiary combination of freeze-dried urine of indian breeds of cow with plant products against snail lymnaea acuminata.snail lymnaea acuminata is the intermediate host of liver fluke fasciola gigantica, which cause endemic fasciolosis among cattle population of eastern uttar pradesh. control of snail population by molluscicides is one of the effective methods to control fasciolosis. in the present study molluscicidal activity of tertiary combination of freeze-dried urine of different indian breeds of cow sahiwal, geer and tharparkar with annona squamosa seed powder, ferula asafoetida root latex, azadirachta indi ...201224199478
[analysis of the microbiological quality and potential presence of listeria monocytogenes in custard apple (annona muricata), mango (mangifera indica) and passion fruit (passiflora edulis) pulps from costa rica].the objective of this work was to determine some of the indicators associated to shelf life, hygiene, process and storage conditions for some of custard apple, mango and passion fruit pulps distributed by the main supermarket chains of the metropolitan area of san josé, costa rica, as well as to examine the potential presence of listeria monocytogenes in them. sixty fruit pulp samples were analyzed. tests included ph determination, total aerobic plate count, yeasts and mold count, lactic bacteri ...201324167958
antiplasmodial activity of medicinal plants from chhotanagpur plateau, jharkhand, india.the alarmingly increasing problem of drug resistance in treatment of malaria has led to an urgent need for identifying new anti-malarial drugs for both prophylaxis and chemotherapy.201525721804
comparative efficacy of annona squamosa and azadirachta indica extracts against boophilus microplus izatnagar isolate.in the search of developing herbal acaricides, eight medicinal plants were screened for their efficacy against boophilus microplus, the widely distributed tick species in india. of the seven extracts screened, the extracts prepared from the annona squamosa seed showed very high level of efficacy (70.8%) after 24 h of treatment. the effect of treatment on oviposition of the survived ticks was also assessed, and a significant reduction (p < 0.05) in the reproductive index was noted in comparison t ...200919557436
control of silverleaf whitefly, cotton aphid and kanzawa spider mite with oil and extracts from seeds of sugar apple.development of alternative methods for pest management is needed with the increased concern for adverse effects of pesticides for human health and the environment. the main goal of our study was to test the oil from seeds of sugar apple (annona squamosa), an edible tropical fruit for pest control. the oil pressed out of seeds was as effective in controlling the silverleaf whitefly, bemisia argentifolii bellows & perring (homoptera: aleyrodidae), infesting leaves of tomato plants in greenhouse co ...200919768275
in vitro antimalarial activity of medicinal plant extracts against plasmodium falciparum.malaria is a major global public health problem, and the alarming spread of drug resistance and limited number of effective drugs now available underline how important it is to discover new antimalarial compounds. in the present study, ten plants were extracted with ethyl acetate and methanol and tested for their antimalarial activity against chloroquine (cq)-sensitive (3d7) and cq-resistant (dd2 and indo) strains of plasmodium falciparum in culture using the fluorescence-based sybr green assay. ...201120809417
antibacterial function of herbal extracts on growth, survival and immunoprotection in the black tiger shrimp penaeus monodon.this study examined the effects of an herbal extract composed of nine herbs i.e aloe vera, andrographis pariculata, annona squamosa, azadirachta indica, citrus aurantifolia, coriandrum sativum, ocimum sanctum, ollium cepa and psidium guajava on growth, survival rate and immunoprotection against pathogenic vibrio harveyi in the tiger shrimp penaeus monodon. the petroleum ether, methanol and n-hexen extracts of different herbal plants were selected, processed and thoroughly mixed in equal proporti ...201728365386
mono-tetrahydrofuran annonaceous acetogenins from annona squamosa as cytotoxic agents and calcium ion chelators.eight new mono-tetrahydrofuran (thf)-type annonaceous acetogenins, squafosacins b, c, f, and g (1-4), squadiolins a-c (5-7), and cis-annotemoyin-1 (8), as well as eight known annonaceous acetogenins, glabranin, annotemoyins-1 and -2, bullatencin, cis-bullatencin, and uvariamicins-i, -ii, and -iii, were isolated from the seeds of annona squamosa by hplc. the structures of all new isolates were elucidated by using spectroscopic and chemical methods. squadiolins a (5) and b (6) showed ng/ml potency ...200818419154
green synthesis of silver nanoparticles for the control of mosquito vectors of malaria, filariasis, and dengue.a biological method was used to synthesize stable silver nanoparticles that were tested as mosquito larvicides against aedes aegypti, anopheles stephensi, and culex quinquefasciatus. annona squamosa leaf broth (5%) reduced aqueous 1 mm agno₃ to stable silver nanoparticles with an average size of 450 nm. the structure and percentage of synthesized nanoparticles was characterized by using ultraviolet spectrophotometry, x-ray diffraction, fourier transform infrared spectroscopy, and scanning electr ...201222022807
larvicidal efficacy of medicinal plant extracts against anopheles stephensi and culex quinquefasciatus (diptera: culicidae).mosquitoes transmit serious human diseases, causing millions of deaths every year. natural products of plant origin with insecticidal properties have been used in recent years for control of a variety of pest insects and vectors. the present study was based on assessments of the larvicidal activity to determine the efficacies of hexane, chloroform, ethyl acetate, acetone and methanol extracts of ten medicinal plants tested against fourth instar larvae of malaria vector, anopheles stephensi listo ...201020962718
an ethnobotanical study of medicinal plants used by traditional healers in silent valley of kerala, india.medicinal plants are treating and preventing a variety disease. there is urgency in recording such data. this is the first ethno botanical study in which statistical calculations about plants are done by icf method. the present study was aimed to identify plants collected for medicinal purposes by the traditional healers of silent valley, located in palakad district of kerala, india and to document the traditional names, preparation and uses of these plants.201424832113
ocular toxicity by seeds of annona squamosa (custard apple).custard apple seeds have been used in native medicine from time immemorial for the management of head lice and skin exfoliation. we report six consecutive patients who developed toxic keratoconjunctivitis within 6-12 h of ocular exposure to custard apple seeds. the use of topical steroid worsens the toxicity and predisposes to the development of microbial keratitis in such cases. patients showed a good response to primary treatment with topical fortified antibiotics and lubricants. this case ser ...201627688289
mineral composition, nutritional properties, total phenolics and flavonoids compounds of the atemoya fruit (annona squamosa l. x annona cherimola mill.) and evaluation using multivariate analysis techniques.the atemoya is a hybrid fruit obtained by crossing of cherimoya (annona cherimola mill.) with sweet sop (annona squamosa l.). the information about chemical composition of atemoya is scarce. the mineral composition was evaluated employing inductively coupled plasma optical emission spectrometry (icp oes) and the centesimal composition and the physico-chemical parameters were assessed employing procedures described in the aoac methods. the total phenolic compounds (tpc) and total flavonoids (tf) ...201627598843
annona squamosa seed extract in the regulation of hyperthyroidism and lipid-peroxidation in mice: possible involvement of quercetin.annona squamosa (custard apple) seeds are generally thrown away as waste materials. the extract of these seeds was evaluated for its possible ameliorative effect in the regulation of hyperthyroidism in mouse model. serum triiodothyronine (t(3)), thyroxine (t(4)) concentrations, hepatic glucose-6-phospatase (g-6-pase) and 5'-mono-deiodinase (5'di) activity were considered as the end parameters of thyroid function. simultaneously hepatic lipid peroxidation (lpo), superoxide dismutase (sod) and cat ...200717291737
antidiabetic and antioxidant activity of annona squamosa extract in streptozotocin-induced diabetic rats.the aim of the study is to analyse the antioxidant effect of oral administration of aqueous extract of annona squamosa (a. squamosa) leaf on blood glucose, haemoglobin, glycosylated haemoglobin, plasma insulin, antioxidant enzymes and lipid peroxidation in liver and kidney to streptozotocin (stz)-induced diabetic rats.200616865205
anthelmintic activity of botanical extracts against sheep gastrointestinal nematodes, haemonchus contortus.the source of chemical anthelmintics (levamisole, flubendazole, and thiabendazole) had limited the success of gastrointestinal nematodiasis control in sheep and goats and thus awakened interest in the study of medicinal plant extracts as alternative sources of anthelmintics. the egg hatching and larvicidal effect of indigenous plant extracts were investigated against the sheep parasite, haemonchus contortus. the purpose of the present study was to assess the efficacy of leaf, bark, and seed ethy ...201021161270
anti-ulcer constituents of annona squamosa twigs.phytochemical investigation of annona squamosa twigs, resulted in isolation and identification of twelve known (1-12) compounds among them one 1-(4-ß-d-glucopyranosyloxyphenyl)-2-(ß-d-glucopyranosyloxy)-ethane (11) is synthetically known but first time isolated from natural sources. their structures were elucidated using 1d and 2d nmr spectroscopic analysis. the isolated compounds (2-8, 11) were evaluated for h(+) k(+)-atpase activity. three of these compounds (+)-o-methylarmepavine (2), n-methy ...201121342663
storage behavior of mango as affected by post harvest application of plant extracts and storage conditions.the use of plant extracts could be a useful alternative to synthetic fungicides in the post harvest handling of fruits and vegetables. the aim of this study was to access the efficacy of extracts obtained from four plants (neem, pongamia, custard apple leaf and marigold flowers) on the extension of shelf life of mango fruits cv. dashehri under two storage conditions (cool store and ambient condition). the fruits were treated with 2 concentrations of each plant extracts (10 % and 20 %) were place ...201425328189
determination of some physicochemical characteristics, bioactive compounds and antioxidant activity of tropical fruits from yucatan, mexico.the aim to the study was to determine the physicochemical composition, bioactive compounds and antioxidant activity of fruits from yucatan, mexico such as star apple, cashew, mombin, mamey sapote, white sapote, sugar apple, sapodilla, dragon fruit, nance, ilama, custard apple, mamoncillo and black sapote. the physicochemical characteristics were different between fruits and were good sources of bioactive compounds. the edible part with the highest values of antioxidant activity were mamoncillo, ...201424444968
influence of gamma irradiation and benzyl adenine on keeping quality of custard apple fruits during storage.the custard apple (annona squamosa) fruits were procured from local market, irradiated with radiation doses 0, 0.25, 0.50, 0.75, 1.00, 1.25, 1.50, 1.75 kgy and then treated with benzyl adenine (50 and 100 part per million) and stored at ambient temperature (25 ± 5 °c, relative humidity 90 ± 2%) for 12 days. the treated fruits were evaluated for sensory (viz; flavour, texture, internal and external colour) and chemical constituents (viz; total soluble solids, titrable acidity, ascorbic acid, free ...201324426000
characterization and gene cloning of an acidic thaumatin-like protein (tlp 1), an allergen from sapodilla fruit (manilkara zapota).allergy to sapodilla (manilkara zapota) fruit ingestion is rare. an independent study from our group has identified a basic thaumatin-like protein (tlp 2) as the major allergen. the present study was aimed at identifying and characterizing additional allergens from sapodilla.201324060761
six cytotoxic annonaceous acetogenins from annona squamosa seeds.custard apple (annona squamosa l.) is an edible tropical fruit, and its seeds had been used in south china as a folk medicine to treat "malignant sore" (cancer) and as an insecticide. phytochemical investigation of the ethanol fraction of custard apple seeds led to the isolation of six new annonaceous acetogenins: annosquacins a-d (1-4), annosquatin a (5) and annosquatin b (6). their structures were elucidated by spectroscopic analysis. compounds 1-4 are adjacent bistetrahydrofuran annonaceous a ...201222953811
custard apple and latex allergy: a new type of cross-reaction? 201222697017
an anti-inflammatory ent-kaurane from the stems of annona squamosa that inhibits various human neutrophil functions.we have previously shown that 11 ent-kauranes isolated from the stems of annona squamosa exhibited immunomodulating effects in leukocytes. in this study, a cellular model using isolated human neutrophils, which are important in the pathogenesis of rheumatoid arthritis, ischemia-reperfusion injury, chronic obstructive pulmonary disease, asthma and other inflammatory diseases, was established in order to elucidate the anti-inflammatory functions of 16beta,17-dihydroxy-ent-kauran-19-oic acid (1). r ...200516254820
protective effect of methanolic extract of annona squamosa linn in isoniazid-rifampicin induced hepatotoxicity in rats.the present study was made to investigate the protective effect of methanolic extract of annona squamosa on isoniazid-rifampicin-induced hepatotoxicity in rats. rats were divided into five different groups (n=6), group 1 served as a control, group 2 received isoniazid (100 mg/kg, i.p.) and co-administered with rifampicin (100 mg/kg, i.p.), in sterile water, group 3 and 4 served as extract treatment groups and received 250 & 500 mg/kg bw, p.o methanolic extract of annona squamosa and group 5 serv ...201121454160
in vitro antioxidant studies of annona squamosa linn. leaves.the free radical scavenging potential of the leaves of a. squamosa was studied by using different antioxidant models of screening. the ethanolic extract at 1000 microg/ml showed maximum scavenging of the radical cation, 2,2-azinobis- (3-ethylbenzothiazoline-6-sulphonate) (abts) observed upto 99.07% followed by the scavenging of the stable radical 1,1-diphenyl, 2-picryl hydrazyl (dpph) (89.77 %) and nitric oxide radical (73.64%) at the same concentration. however, the extract showed only moderate ...200415573531
inhibitory effects of ent-kauranes from the stems of annona squamosa on superoxide anion generation by human neutrophils.eleven ent-kauranes, isolated from the fresh stems of annona squamosa l. (annonaceae), were subjected to assays on the generation of superoxide anion (o2.--) by human neutrophils. except for ent-kaur-16-en-19-oic acid, 16beta,17-dihydroxy- ent-kauran-19-al, and 16alpha,17-dihydroxy -ent-kauran-19-al, all ent-kauranes showed significant inhibitory effect on o2.-- generation in response to formyl- l-methionyl- l-leucyl- l-phenylalanine (fmlp/cb). in contrast, phorbol myristate acetate (pma)-induce ...200415114504
antimicrobial and pesticidal activity of partially purified flavonoids of annona squamosa.foliar extracts of annona squamosa (family: annonaceae) were screened for antimicrobial and insecticidal activity against the common microbial infestants of pulses and the stored grain pest pulse beetle, callosobruchus chinensis (coleoptera: bruchidae). flavonoids isolated from aqueous extracts of a squamosa showed antimicrobial activity against all the common microbial contaminants of pulses and 80% insecticidal activity against c chinensis at a concentration of 0.07 mg ml-1. various physico-ch ...200211838282
three new bioactive bis-adjacent thf-ring acetogenins from the bark of annona squamosa.continuing work on the bioactivity-directed fractionation of the bark of annona squamosa has resulted in the discovery of three new annonaceous acetogenins, (2,4-cis and trans)-squamolinone (1), (2,4-cis and trans)-9-oxoasimicinone (2), and bullacin b (3). compounds 1-3 are all adjacent bis-thf ring acetogenins with 2 representing the first bis-ring acetogenin to contain a carbonyl along its aliphatic chain. compound 3 was selectively cytotoxic in a panel of six human tumor cell lines with a pot ...19989629470
antidiabetic and antioxidative effects of annona squamosa leaves are possibly mediated through quercetin-3-o-glucoside.present investigation was made to reveal the involvement of a quercetin in the antidiabetic and antiperoxidative effects of annona squamosa leaf extract. quercetin-3-o-glucoside (characterized by uv, ir, ms and nmr analyses) was isolated from annona squamosa leaves and examined for its potential to regulate alloxan-induced hyperglycemia and lipid peroxidation (lpo) in rats. while in alloxan treated animals, an increase in the concentration of serum glucose with a parallel decrease in insulin lev ...200718997283
first report of lecanodiaspis dendrobii douglas, 1892 (hemiptera: lecanodiaspididae) and the associated parasitoid cephaleta sp. (hymenoptera: pteromalidae) in brazil.lecanodiaspis dendrobii douglas, 1892 (hemiptera: coccoidea: lecanodiaspididae) and the associated parasitoid cephaleta sp. (hymenoptera: pteromalidae) are reported for the first time in brazil. specimens of this scale insect were collected on branches and stems of acacia mangium willd., leucaena leucocephala (lam.) de wit (fabaceae), morus nigra l. (moraceae), citrus reticulata blanco (rutaceae), tectona grandis l. f. (verbenaceae), anacardium occidentale (anacardiaceae), annona squamosa l. and ...201626871743
large scale screening of ethnomedicinal plants for identification of potential antibacterial compounds.the global burden of bacterial infections is very high and has been exacerbated by increasing resistance to multiple antibiotics. antibiotic resistance leads to failed treatment of infections, which can ultimately lead to death. to overcome antibiotic resistance, it is necessary to identify new antibacterial agents. in this study, a total of 662 plant extracts (diverse parts) from 222 plant species (82 families, 177 genera) were screened for antibacterial activity using the agar cup plate method ...201626985889
n-methyl-6, 7-dimethoxyisoquinolone in annona squamosa twigs is the major immune modifier to elicit polarized th1 immune response in balb/c mice.annona squamosa (as) has traditionally been used as ethnomedicine. we have earlier extracted and fractionated the twigs of as based upon its bioactivity and observed its immune potentiating activity that was localized in its three fractions. present communication deals with the phytochemical analysis and pharmacological investigation of the most active chloroform fraction that led to isolation and identification of a number of compounds whose structures were elucidated using 1d and 2d nmr spectr ...201222004725
evaluation of enzymes inhibition activities of medicinal plant from burkina faso.the aim of the present study was to evaluate some enzymes inhibitory effects of 11 plant species belonging to 9 families from burkina faso. methanolic extracts were used for their glutathione-s-transferase (gst), acetylcholinesterase (ache), carboxylesterase (ces) and xanthine oxidase (xo) inhibitory activities at final concentration of 100 microg ml(-1). the total phenolics, flavonoids and tannins were also determined spectrophotometrically using folin-ciocalteu, alcl3 and ammonium citrate iron ...201121916259
efficacy of annona squamosa on wound healing in streptozotocin-induced diabetic rats.annona squamosa l. (annonaceae), commonly known as custard apple, mainly used for its edible fruit, is also recognised with numerous medicinal properties. as there is no report on the efficacy of this plant for wound healing, we examined the efficacy of ethanolic extract of a. squamosa leaves on wound repair in streptozotocin-nicotinamide-induced diabetic rats. open excision wounds were made on the back of rats. the drug at a dosage of 100 mg/kg body wt was reconstituted in 200 µl of phosphate b ...201222233431
incidence and growth of salmonella enterica on the peel and pulp of avocado (persea americana) and custard apple (annona squamosa).the aim of this study was to assess the incidence and to estimate the growth kinetic parameters (maximum growth rate, μ; lag time, λ; and maximum population, κ) of salmonella on the peel and pulp of avocado (perseaamericana var. americana) and custard apple (annona squamosa l.) as affected by temperature (10-30°c). the incidence of salmonella was assessed on the peel and pulp of the fruits (n=200 of each fruit), separately, totalizing 800 analyses. only three samples of custard apple pulp were p ...201627393884
leishmanicidal activity and cytotoxicity of compounds from two annonacea species cultivated in northeastern brazil.visceral leishmaniasis is endemic in 88 countries, with a total of 12 million people infected and 350 million at risk. in the search for new leishmanicidal agents, alkaloids and acetogenins isolated from leaves of annona squamosa and seeds of annona muricata were tested against promastigote and amastigote forms of leishmania chagasi.201122031071
Cytotoxic bistetrahydrofuran annonaceous acetogenins from the seeds of Annona squamosa.Three new bistetrahydrofuran annonaceous acetogenins (1-3) were isolated from a 95% EtOH extract of Annona squamosa seeds. Four known annonaceous acetogenins, uvarigrandin A (4), bullatacin (5), squamostatin-A (6), and squamostatin-D (7), were also isolated. Their structures were elucidated by spectroscopic analyses, and the absolute configurations of the carbinol centers of 1-3 were defined by application of the Mosher method. These compounds all exhibited significant cytotoxic activity in vitr ...201122011319
extracellular synthesis of silver nanoparticles by novel pseudomonas veronii as41g inhabiting annona squamosa l. and their bactericidal activity.in present investigation extracellular synthesis of silver nanoparticles were synthesized using cell free supernatant of pseudomonas veronii as41g isolated from annona squamosa l. the bacterium significantly reduced silver nitrate to generate silver nanoparticles which was characterized with hyphenated techniques. synthesis of silver nanoparticles preliminary confirmed by uv-visible spectrophotometry with the intense peak at 410nm, further ftir analysis revealed the possible role of biomolecules ...201525459703
biosynthesis of gold nanoparticles by pseudomonas veronii as41g inhabiting annona squamosa l.biogenic principles to nanotechnology have generated tremendous attention in recent past owing eco friendly benign process for synthesis of nanoparticles. present investigation reports extracellular synthesis of gold nanoparticles using cell free supernatant of pseudomonas veronii as 41g, a novel endophyte isolated from annona squamosa l. gold nanoparticles formation was confirmed with uv-visible spectrophotometer. ftir analysis predicted various functional groups responsible for reduction of me ...201526093965
ige-mediated hypersensitivity to custard-apple. 19979201378
haploid plants from in vitro anther culture of annona squamosa linn.haploid plants were induced from anther callus of annona squamosa linn. (custard apple) on a nitsch basal medium supplemented with 6-benzyl-aminopurine and naphthalene acetic acid. when naphthalene acetic acid was replaced by indole-3-acetic acid only multiple shoots were obtained. pretreatment (chilling, centrifugation, reduced atmospheric pressure etc.) of the flowers was not effective but dissection of the flowers in a suspension of activated charcoal and sucrose was found essential. the anth ...198324258051
application of low-pressure gas chromatography/tandem mass spectrometry to the determination of pesticide residues in tropical fruits.a multiresidue method has been developed for determining pesticide residues in the tropical fruits kiwi, custard apple, and mango. the intended purpose of the method is for regulatory analyses of commodities for pesticides that have established maximum residue limits. a fast and simple extraction method with cyclohexane-ethyl acetate (1 + 1, v/v) and a high-speed homogenizer was optimized. pressurized liquid extraction was evaluated as an alternative automated extraction technique. the pesticide ...201217760354
ascorbic acid contents of pakistani fruits and vegetables.fresh fruits and vegetables are good sources of vitamin c which is known for its antioxidant and immune-enhancing effects. the objective of this study was to determine ascorbic acid (vitamin c) contents of regularly consumed fruits and vegetables available in pakistani markets. most commonly used fresh fruits and vegetables were homogenized in 5% trichloroacetic acid, and ascorbic acid contents in the extracts were determined using a spectrophotometric method. banana, custard apple, orange, lemo ...200617105704
latex-vegetable syndrome due to custard apple and aubergine: new variations of the hevein symphony.an increasing number of vegetables with crossreactions to latex are being described in patients with latex-vegetable syndrome. we present two of these vegetables, custard apple linked in two previous cases with latex sensitisation, and aubergine, that had not been described up to now in patients with latex sensitisation. the diagnosis of both cases was based on the clinical history, positive skin prick test (spt) and specific ige to the offending vegetables, as well as to positive spt and specif ...200516433216
rain forest provides pollinating beetles for atemoya crops.small beetles, usually species of nitidulidae, are the natural pollinators of atemoya (annona squamosa l. x a. cherimola mill. hybrids; custard apple) flowers but commercial atemoya growers often need to carry out labor-intensive hand pollination to produce enough high-quality fruit. because australian rain forest has plant species in the same family as atemoya (annonaceae) and because many rain forest plants are beetle pollinated, we set out to discover whether tropical rain forest in far north ...200516156571
antitumour activity of annona squamosa seed extracts is through the generation of free radicals and induction of apoptosis.the plant, annona squamosa traditionally known as custard apple possesses potent bioactive principles in all its parts. the effect of aqueous and organic extracts from defatted seeds of a. squamosa was studied on a rat histiocytic tumour cell line, ak-5. both the extracts caused significant apoptotic tumour cell death with enhanced caspase-3 activity, down regulation of antiapoptotic genes bcl-2 and bcl(xl), and enhanced the generation of intracellular ros, which correlated well with the decreas ...200422900348
oral allergic syndrome after ingestion of custard apple. 200312653803
anti-headlice activity of a preparation of annona squamosa seed extract.various parts of annona squamosa linn. (custard apple) have long been used in thai folk medicine. the effectiveness of organic solvent extracts of custard apple seeds and leaves against headlice has been reported. the present study is focused on the efficacy and stability of seed extract prepared as a cream. a petroleum ether extract of seeds was prepared as a 20% w/w oil in water cream and tested for anti-headlice activity in school girls. after a 3 hour-hair exposure to 20 g of freshly prepare ...200011414452
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