| sequence data suggests big liver and spleen disease virus (blsv) is genetically related to hepatitis e virus. | a monoclonal antibody (mab) that reacted specifically with a 16 kda big liver and spleen disease virus (blsv) protein was used to identify the protein in western immunoblots of infected liver extracts and enable partial amino acid sequence analysis of the protein. based on this sequence, a degenerate primer was designed that was used in conjunction with random hexamers in a reverse transcriptase-por (rt pcr), to amplify a 523 bp product from rna extracted from homogenates of blsv-infected livers ... | 1999 | 10501168 |
| the putative capsid protein of the newly identified avian hepatitis e virus shares antigenic epitopes with that of swine and human hepatitis e viruses and chicken big liver and spleen disease virus. | we recently identified a novel virus, designated avian hepatitis e virus (avian hev), from chickens with hepatitis-splenomegaly (hs) syndrome in the usa. we showed that avian hev is genetically related to swine and human hevs. here we report the antigenic cross-reactivity of the putative open reading frame 2 (orf2) capsid protein of avian hev with those of swine and human hevs and the australian chicken big liver and spleen disease virus (blsv). the region encoding the c-terminal 268 amino acid ... | 2002 | 12185274 |
| heterogeneity and seroprevalence of a newly identified avian hepatitis e virus from chickens in the united states. | we recently identified and characterized a novel virus, designated avian hepatitis e virus (avian hev), from chickens with hepatitis-splenomegaly syndrome (hs syndrome) in the united states. avian hev is genetically related to but distinct from human and swine hevs. to determine the extent of genetic variation and the seroprevalence of avian hev infection in chicken flocks, we genetically identified and characterized 11 additional avian hev isolates from chickens with hs syndrome and assessed th ... | 2002 | 12409397 |