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helminth survey of wildcats in japan.two iriomote cats (felis iriomotensis) and 2 tsushima leopard cats (felis bengalensis euptilura) killed probably by traffic accidents were submitted to the helminthological examination. in iriomote cats, 8 species of parasites (spirometra erinacei, toxocara cati, molineus springsmithi, uncinaria maya, capillaria aerophila, c. felis-cati, larvae of an unidentifiable lung worm and one species of acanthocephala) were found. in tsushima leopard cats, 10 species of parasites (pharyngostomum cordatum, ...19947696394
helminths of the tsushima leopard cat (felis bengalensis euptilura).three tsushima leopard cats (felis bengalensis euptilura) in japan were collected as road kills. three species of trematodes (pharyngostomum cordatum, paragonimus sp. and dicrocoeliidae gn. sp.), one species of cestode (spirometra erinacei) and nine species of nematodes (arthrostoma hunanensis, uncinaria felidis, uncinaria sp., ancylostoma tubaeforme, molineus springsmithi, toxocara cati, capillaria aerophila, capillaria felis-cati, and capillaria sp.) were found. among these helminths, arthrost ...19938445779
shedding of toxoplasma gondii oocysts by felidae in zoos in the czech republic.in 1995 and 1996, the shedding of toxoplasma gondii oocysts was monitored in the faeces of felidae in six zoos in the czech republic. in all, 2287 samples of faeces from 19 species of felidae were examined. in ostrava zoo, four episodes of shedding of toxoplasma-like oocysts were identified, using a flotation examination, in a pair of wild cats (felis silvestris), six episodes in a wild cat held separately, and three episodes in a pair of amur leopard cats (f. euptilurus). after the passage of s ...19989493305
interspecies transmission of feline immunodeficiency virus from the domestic cat to the tsushima cat (felis bengalensis euptilura) in the wild.feline immunodeficiency virus (fiv) was isolated from a wild-caught tsushima cat (felis bengalensis euptilura), an endangered japanese nondomestic subspecies of leopard cat (f. bengalensis). phylogenetic analysis of the env gene sequences indicated that the fiv from the tsushima cat belonged to a cluster of subtype d fivs from domestic cats. fivs from both the tsushima cat and the domestic cat showed similar levels of replication and cytopathicity in lymphoid cell lines derived from these two sp ...199910438892
genetic diversity in domestic cats felis catus of the tsushima islands, based on mitochondrial dna cytochrome b and control region nucleotide sequences.nucleotide sequences of mitochondrial dna (mtdna) of 50 domestic cats (felis catus) obtained from the tsushima islands were determined and the genetic diversity was analyzed. in the cats, six haplotypes of the complete cytochrome b sequences (1,140 base-pairs, bp) and ten haplotypes of the partial control region sequences (350 bp) were identified. haplotypes obtained from both genes showed existence of at least 11 maternal lineages of domestic cats in tsushima. mean values of polymorphic site nu ...200515988156
hepatozoonosis in two species of japanese wild cat.hepatozoon sp. infections were detected in two species of japanese wild cat, iriomote wild cat (felis iriomotensis) and tsushima leopard cat (felis bengalensis euptilura), between april 1993 and october 2005. the prevalence was 56.7% (17/30) and 14.3% (6/42), respectively. the most affected organ was the heart; all infected animals had organisms in their hearts. the parasitizing form was schizont and various developmental stages were observed. the size of schizont and merozoite was 22.3 +/- 3.1 ...200616953084
risk analysis of feline immunodeficiency virus infection in tsushima leopard cats (prionailurus bengalensis euptilurus) and domestic cats using a geographic information system.in this study, based on the data from fiv screening surveys of captive cats conducted by the kyushu veterinary union and collaborators as part of the infection control program for tsushima leopard cats (prionailurus bengalensis euptilurus), we elucidated the spatial distribution of fiv-positive individuals among leopard cats and domestic cats using a geographic information system. data from fiv screening surveys carried out among 86 leopard cats (1996-2006) and 713 captive domestic cats (2001-20 ...201020379084
fecal steroid metabolites and reproductive monitoring in a female tsushima leopard cat (prionailurus bengalensis euptilurus).although the tsushima leopard cat (prionailurus bengalensis euptilurus) is one of the most endangered mammals in japan, its reproductive physiology and endocrinology have been not elucidated. the objective was to establish the non-invasive monitoring of reproductive endocrinology in a female tsushima leopard cat and to identify the types of fecal reproductive steroid metabolites in this species. fecal concentrations of estrogen and progestin were determined by enzyme immunoassays, from 60 d befo ...201020615533
The complete mitochondrial genome sequence of the Amur leopard cat, Prionailurus bengalensis euptilurus.The total length of the Prionailurus bengalensis euptilurus genome is 16,990 bp, with a total base composition of 33% A, 27.4% T, 26% C, and 13.5% G. The base compositions present clearly the A-T skew, which is most obvious in the tRNA genes (64%).201122040073
anaplasma sp. and hemoplasma infection in leopard cats (prionailurus bengalensis euptilurus) from korea.this study examined the occurrence of anaplasma spp. and hemoplasma infection in leopard cats, prionailurus bengalensis euptilurus, in korea. twenty-nine biological samples were tested by molecular analysis. two (6.9%) and eight (27.6%) tested specimens were positive for anaplasma bovis and hemoplasma infection, respectively. based on our results, anaplasma/ehrlichia spp. and hemoplasma are regularly infecting leopard cat populations of korea. considering their endangered status, regular monitor ...201526040618
molecular survey of arthropod-borne pathogens in ticks obtained from japanese wildcats.the iriomote cat (ic), prionailurus bengalensis iriomotensis, and the tsushima leopard cat (tlc), prionailurus bengalensis euptilurus, are endangered subspecies of leopard cats in japan. in addition to habitat destruction and road kills, infectious diseases may threaten their populations, and infection with arthropod-borne pathogens has been reported in both subspecies. infestations with ectoparasites, especially ticks, have frequently been observed in ics and tlcs. in the present study, ticks c ...201525682495
epidemiological survey of tick-borne protozoal infection in iriomote cats and tsushima leopard cats in japan.this epidemiological survey was conducted to determine the prevalence of hepatozoon, babesia and theileria infection in the iriomote cat (ic) and the tsushima leopard cat (tlc). blood samples from 43 ics and 14 tlcs were collected between november 2002 and january 2012. polymerase chain reaction and dna sequencing analyses detected a hepatozoon felis infection prevalence of 72.0% (31/43 cats) and 100% (14/14 cats) in ics and tlcs, respectively. the degree of hepatozoon parasitemia observed on bl ...201323449464
the presence of tick-borne diseases in domestic dogs and cats living on iriomote-jima and tsushima islands.the iriomote cat and tsushima leopard cat are endangered wildcats in japan and inhabit only iriomote-jima and tsushima islands, respectively. domestic dogs and cats living on iriomote-jima and tsushima islands were surveyed to clarify the interrelationship between wildcats and domestic animals regarding tick-borne disease transmission. pathogen-derived dna in blood samples was detected by polymerase chain reaction. babesia gibsoni was detected in dogs of iriomote-jima, and hepatozoon felis and h ...201728484147
epidemiologic survey of feline leukemia virus in domestic cats on tsushima island, japan: management strategy for tsushima leopard cats.the tsushima leopard cat (tlc) prionailurus bengalensis euptilurus, a subspecies of p. bengalensis, is designated a national natural monument of japan, and lives only on tsushima island, nagasaki prefecture, japan. tlcs are threatened by various infectious diseases. feline leukemia virus (felv) causes a serious infectious disease with a poor prognosis in cats. therefore, the transmission of felv from tsushima domestic cats (tdcs) to tlcs may threaten the tlc population. we investigated the felv ...201728782421
ebstein anomaly in the tsushima leopard cat (prionailurus bengalensis euptilurus).ebstein anomaly is a rare congenital heart disease that has been described in domestic dogs, a meerkat, a pygmy goat, and a lion. an 11-mo-old tsushima leopard cat presented to osaka prefecture university veterinary hospital for diagnosis and treatment of right-sided congestive heart failure. echocardiography showed a dilated right atrium and ventricle with an enlarged tricuspid valve annulus and apical displacement of the tricuspid valve leaflets. the cat was diagnosed with ebstein anomaly. to ...201728749297
molecular identification of avian haemosporidia in wild birds and mosquitoes on tsushima island, japan.we investigated for the first time the prevalence of avian haemosporidia of genera plasmodium, haemoproteus and leucocytozoon among birds and mosquitoes on tsushima island of japan, which is located between japan and the korean peninsula. of 55 wild birds belonging to 33 species, 16 (29.1%) tested positive for haemosporidia as follows: plasmodium spp. (11/55; 20.0%); haemoproteus spp. (2/55; 3.6%); and leucocytozoon spp. (3/55; 5.5%). a genetic lineage isolated from the eurasian sparrowhawk (acc ...201323123824
[new evidence for the spread of thelazia callipaeda in the far east].thelazia callipaeda nematodes parasitize in the eyes of domestic and wild carnivorous mammals (more often in canidae). numerous cases of human infestation are also known. the nematode spreads in south and east asia although in the last decade this has been reported from dogs, cats and wolves in south and central europe as well. in the russian federation, t. callipaeda was earlier observed in dogs, cats, foxes and raccoon dogs in some regions of the russian far east. two cases of human infestatio ...201525850318
molecular epidemiologic survey of bartonella, ehrlichia, and anaplasma infections in japanese iriomote and tsushima leopard cats.the iriomote cat (ic; prionailurus iriomotensis) and the tsushima leopard cat (tlc; prionailurus bengalensis euptilura) are endangered wild felids in japan. as a part of ongoing conservation activities, we conducted a molecular, epidemiologic survey of bartonella, ehrlichia, and anaplasma infections in wild ic and tlc populations. blood samples (47 from 33 individual ic; 22 from 13 tlc) were collected between august 2002 and january 2011. using pcr analysis, we confirmed the presence of bartonel ...201323778615
metabolism of prostaglandin f2alpha in eurasian lynx (lynx lynx) and asian leopard cat (prionailurus bengalensis euptilura).methods for monitoring endocrine status are useful tools for reproduction management. in particular, successful captive breeding of endangered feline species requires reliable methods for pregnancy diagnosis. in many species, uterine and placental prostaglandin-f2α (pgf2α) is involved in the regulation of reproductive processes. pgf2α is metabolized to 13,14-dihydro-15-keto-pgf2a (pgfm) during the first passage through the lungs. immunoreactive pgfm is elevated in pregnant felids during the last ...201728111808
neurofibrillary tangles and the deposition of a beta amyloid peptide with a novel n-terminal epitope in the brains of wild tsushima leopard cats.beta amyloid (aβ) deposits are seen in aged individuals in many of the mammalian species that possess the same aβ amino acid sequence as humans. conversely, neurofibrillary tangles (nft), the other hallmark lesion of alzheimer's disease (ad), are extremely rare in these animals. we detected aβ deposits in the brains of tsushima leopard cats (prionailurus bengalensis euptilurus) that live exclusively on tsushima island, japan. aβ42 was deposited in a granular pattern in the neuropil of the pyrami ...201223056312
a trial of semen collection by transrectal electroejaculation method from amur leopard cat (prionailurus bengalensis euptilurus).we collected semen from a male amur leopard cat using the transrectal electroejaculation method and investigated the semen qualities for about four years. in addition, the influence of the season on the spermatogenic function of the amur leopard cat was investigated with regard to the semen qualities, testicular volume and serum testosterone level. as a result, we could collect semen with good sperm qualities that would be useable for artificial insemination. some seasonality was noted in the te ...201626935841
intrauterine insemination with fresh semen in amur leopard cat (pionailurus bengalensis eutilura) during non-breeding season.equine and human chorionic gonadotropins were administered to two female amur leopard cats to induce estrus and ovulation during non-breeding season. fresh semen collected from male cats was surgically inseminated into the uterine horn of the females. in one animal, two fetal sacs without heartbeats were observed on abdominal ultrasonography 31 days after insemination, which indicated that embryo death had occurred. in the other animal, fetal heartbeats were detected in two fetal sacs 29 days af ...201727733725
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