| description of enterobacter ludwigii sp. nov., a novel enterobacter species of clinical relevance. | a new species, enterobacter ludwigii, is presented on the basis of the characteristics of 16 strains, which were isolated from clinical specimens. these bacteria form a distinct genetic cluster in phylogenetic analyses of the population structure of the enterobacter cloacae complex. as determined by dna-dna cross-hybridization experiments in microplates, this genetic cluster can be delineated from the other species of the e. cloacae complex with deltatm values equal to or above 5 degrees c with ... | 2005 | 15900967 |
| exopolysaccharide production by nitrogen-fixing bacteria within nodules of medicago plants exposed to chronic radiation in the chernobyl exclusion zone. | nitrogen-fixing bacteria isolated from root nodules of medicago plants growing in the 10 km zone around the chernobyl nuclear power plant were screened for the production of new water-soluble acidic exopolysaccharides (epss). the different strains belonged to the enteriobacteriaceae family (enterobacter ludwigii, raoultella terrigena, klebsiella oxytoca), except for one which belonged to the rhizobiaceae family (sinorhizobium meliloti). all of the bacteria produced highly viscous eps with an ave ... | 2010 | 20080178 |
| [diversity of associated nitrogen-fixing bacteria isolated from the pioneer plants-vetiver zizanioides]. | vetiver zizanioides is a perennial grass of the poaceae family, known of its silage, soil and water conservation role. the aim of the study was to collect and identify the resources of the nitrogen-fixing bacteria associated with vetiver zizanioides. | 2009 | 20112669 |
| comparison of microbial diversity of edible flowers and basil grown with organic versus conventional methods. | the consumption and use of edible flowers as food is growing; however, no study has been conducted to evaluate their role in the cause of food-borne illness or in food safety. recent food-borne outbreaks traced to fresh herbs have raised concern about their processing and handling. basil, one of the most commonly used fresh herbs, has been identified as a source of food-borne illness. baseline assessments of microflora were performed, and the microbial diversity between growing methods (organic ... | 2010 | 21076485 |
| bacterial species associated with sound and botrytis-infected grapes from a greek vineyard. | grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. in the present study, bacterial species associated with sound and botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. isolates were identified by pcr-denaturing gradient gel electrophoresis (pcr-dgge) and sequence analysis of partial 16s rrna gene. comparable counts were recorded between botrytis-infected and sound grape samples. in ... | 2011 | 21315469 |
| a multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion. | this study used a multiphasic approach, characterized by the simultaneous use of culture-dependent and culture-independent methods, to investigate endophytic bacterial communities in strawberry (fragaria ananassa) fruit. a total of 92 bacterial endophytes were isolated and initially grouped by their repetitive extragenic palindromic (rep)-pcr banding pattern and biochemical features. phylogenetic analysis of the 16s rrna gene sequences of 45 representatives showed that the isolates belonged to t ... | 2011 | 21837472 |
| hydrocarbon degradation, plant colonization and gene expression of alkane degradation genes by endophytic enterobacter ludwigii strains. | the genus enterobacter comprises a range of beneficial plant-associated bacteria showing plant growth promotion. enterobacter ludwigii belongs to the enterobacter cloacae complex and has been reported to include human pathogens but also plant-associated strains with plant beneficial capacities. to assess the role of enterobacter endophytes in hydrocarbon degradation, plant colonization, abundance and expression of cyp153 genes in different plant compartments, three plant species (italian ryegras ... | 2011 | 21700373 |
| environmental microbiota represents a natural reservoir for dissemination of clinically relevant metallo-beta-lactamases. | a total of 10 metallo-β-lactamase-producing isolates of six different species, including brevundimonas diminuta (n = 3), rhizobium radiobacter (n = 2), pseudomonas monteilii (n = 1), pseudomonas aeruginosa (n = 2), ochrobactrum anthropi (n = 1), and enterobacter ludwigii (n = 1), were detected in the sewage water of a hospital. the presence of bla(vim-13) associated with a tn1721-class 1 integron structure was detected in all but one of the isolates (e. ludwigii, which produced vim-2), and in tw ... | 2011 | 21859934 |
| a dual approach employing maldi-tof ms and real-time pcr for fast species identification within the enterobacter cloacae complex. | a real-time pcr procedure targeting the gene of the molecular cochaperon dnaj (dnaj) was developed for specific detection of strains belonging to the enterobacter cloacae group. the inclusivity and exclusivity of the real-time pcr assay were assessed with seven reference strains of e. cloacae, 12 other enterobacter species and 41 non-enterobacter strains. inclusivity as well as exclusivity of the duplex real-time pcr was 100%. in contrast, resolution of matrix-assisted laser desorption ionizatio ... | 2011 | 22150997 |
| structural features and bioremediation activity of an exopolysaccharide produced by a strain of enterobacter ludwigii isolated in the chernobyl exclusion zone. | the bacterium enterobacter ludwigii ez-185-17, member of the family enterobacteriaceae, was isolated from the root nodules of plants harvested in the nuclear power region of chernobyl. under batch culture conditions, the bacteria produce a high-molecular-mass exopolysaccharide (eps). after purification, the structure of this eps was determined using a combinatory approach including monosaccharide composition (gc-fid, hpaec-pad) and branching structure determination (gc-ms), as well as 1d/2d nmr ... | 2012 | 23465914 |
| characterization of exoelectrogenic bacteria enterobacter strains isolated from a microbial fuel cell exposed to copper shock load. | microorganisms capable of generating electricity in microbial fuel cells (mfcs) have gained increasing interest. here fourteen exoelectrogenic bacterial strains were isolated from the anodic biofilm in an mfc before and after copper (cu) shock load by hungate roll-tube technique with solid ferric (iii) oxide as an electron acceptor and acetate as an electron donor. phylogenetic analysis of the 16s rrna gene sequences revealed that they were all closely related to enterobacter ludwigii dsm 16688t ... | 2014 | 25412475 |
| characterization of a novel putative xer-dependent integrative mobile element carrying the bla(nmc-a) carbapenemase gene, inserted into the chromosome of members of the enterobacter cloacae complex. | an enterobacter ludwigii strain was isolated during routine screening of a japanese patient for carriage of carbapenem-resistant enterobacteriaceae. pcr analysis revealed the blanmc-a carbapenemase gene. whole-genome sequencing revealed that blanmc-a was inserted in the chromosome and associated with a novel 29.1-kb putative xer-dependent integrative mobile element, named eludimex-1. bioinformatic analysis identified similar elements in the genomes of an enterobacter asburiae strain and of other ... | 2015 | 26248383 |
| degradation of norgestrel by bacteria from activated sludge: comparison to progesterone. | natural and synthetic progestagens in the environment have become a concern due to their adverse effects on aquatic organisms. laboratory studies were performed to investigate aerobic biodegradation of norgestrel by bacteria from activated sludge in comparison with progesterone, and to identify their degradation products and biotransformation pathways. the degradation of norgestrel followed first order reaction kinetics (t1/2 = 12.5 d), while progesterone followed zero order reaction kinetics (t ... | 2013 | 23952780 |
| diversity and potential application of endophytic bacteria in ginger. | here, 248 endophytic bacterial strains were isolated to assess the distribution and population diversity of endophytic bacteria in ginger plants. a total of 10.4 x 10(4) to 20.2 x 10(4) cfu/g fresh weight endophytic bacteria of different growth stages were isolated. maximum bacterium numbers were obtained at the seedling stage. a total of 107 functional strains were screened, including 17 antibacterial strains and 90 indole acetic acid-producing strains. based on 16s rdna sequence restriction fr ... | 2014 | 25062479 |
| [screening probiotic endophytic bacteria from medicinal plant flex cornuta and the phytopathogen-inhibiting effect]. | culturable endophytic bacteria were isolated from medicinal plant ilex cornuta by plate-spreading method, strains with strong inhibitory effect on phytopathogen were screened by confrontation culture and fermentation filtrate culture methods, and the morphological changes of phytopathogen hyphae treated with endophytic bacteria were examined by microscopy and micrograph. their phylogenetic relationships were determined by homology analysis of the 16s rdna sequences of pcr products and the taxono ... | 2015 | 26571677 |
| diversity, antimicrobial and antioxidant activities of culturable bacterial endophyte communities in aloe vera. | twenty-nine culturable bacterial endophytes were isolated from surface-sterilized tissues (root, stem and leaf) of aloe vera and molecularly characterized to 13 genera: pseudomonas, bacillus, enterobacter, pantoea, chryseobacterium, sphingobacterium, aeromonas, providencia, cedecea, klebsiella, cronobacter, macrococcus and shigella. the dominant genera include bacillus (20.7%), pseudomonas (20.7%) and enterobacter (13.8%). the crude and ethyl acetate fractions of the metabolites of six isolates, ... | 2015 | 26454221 |
| plant growth-promotion and biofortification of chickpea and pigeonpea through inoculation of biocontrol potential bacteria, isolated from organic soils. | seven strains of bacteria [pseudomonas plecoglossicida sri-156, brevibacterium antiquum sri-158, bacillus altitudinis sri-178, enterobacter ludwigii sri-211, e. ludwigii sri-229, acinetobacter tandoii sri-305 and pseudomonas monteilii sri-360; demonstrated previously for control of charcoal rot disease in sorghum and plant growth-promotion (pgp) in rice] were evaluated for their pgp and biofortification traits in chickpea and pigeonpea under field conditions. when treated on seed, the seven sele ... | 2016 | 27833841 |
| evaluation of bacteria isolated from rice rhizosphere for biological control of charcoal rot of sorghum caused by macrophomina phaseolina (tassi) goid. | a total of 360 bacteria, isolated from the rhizospheres of a system of rice intensification (sri) fields, were characterized for the production of siderophore, fluorescence, indole acetic acid (iaa), hydrocyanic acid (hcn) and solubilization of phosphorus. of them, seven most promising isolates (sri-156, -158, -178, -211, -229, -305 and -360) were screened for their antagonistic potential against macrophomina phaseolina (causes charcoal rot in sorghum) by dual culture assay, blotter paper assay ... | 2011 | 25187130 |
| [endophytic bacterial diversity of wild soybean (glycine soja) varieties with different resistance to soybean cyst nematode (heterodera glycines)]. | the aim of this study was to investigate endophytic bacterial diversity of wild soybean varieties with different resistance to soybean cyst nematode(heterodera glycines) , for deciphering the interactions of soybean cyst nematode with endophytic bacteria. | 2014 | 25345025 |
| [characteristics of microbial community structure during isolation of electrical active bacteria]. | to investigate the effect of selective culturing on microorganisms and functional role of electrical active bacteria in biofilm, some exoelectrogens were isolated from microbial fuel cell (mfc) anodic biofilm using hungate roll-tube technique with iron oxide as indicator. at the same time, the dynamics of the microbial community structure was monitored during the pure culture isolation. the results show that maximum voltages of mfcs feeding with lactic acid, acetic acid and steroid wastewater ar ... | 2014 | 25693405 |
| enterobacter muelleri sp. nov., isolated from the rhizosphere of zea mays. | a beige-pigmented, oxidase-negative bacterial strain (jm-458t), isolated from a rhizosphere sample, was studied using a polyphasic taxonomic approach. cells of the isolate were rod-shaped and stained gram-negative. a comparison of the 16s rrna gene sequence of strain jm-458t with sequences of the type strains of closely related species of the genus enterobacter showed that it shared highest sequence similarity with enterobacter mori (98.7 %), enterobacter hormaechei (98.3 %), enterobacter cloaca ... | 2015 | 26294947 |
| enterobacter xiangfangensis sp. nov., isolated from chinese traditional sourdough, and reclassification of enterobacter sacchari zhu et al. 2013 as kosakonia sacchari comb. nov. | a gram-stain-negative bacterial strain, 10-17(t), was isolated from traditional sourdough in heilongjiang province, china. the bacterium was characterized by a polyphasic approach, including 16s rrna gene sequence analysis, rna polymerase β subunit (rpob) gene sequence analysis, dna gyrase (gyrb) gene sequence analysis, initiation translation factor 2 (infb) gene sequence analysis, atp synthase β subunit (atpd) gene sequence analysis, fatty acid methyl ester analysis, determination of dna g+c co ... | 2014 | 24824638 |
| isolation, screening, characterization, and selection of superior rhizobacterial strains as bioinoculants for seedling emergence and growth promotion of mandarin orange (citrus reticulata blanco). | mandarin orange (mo) is an important fruit crop of tropical and subtropical regions of the world. a total of 217 morphologically distinct rhizobacteria from mo orchards in 3 states of northeastern india were isolated and analyzed for 4 plant-growth-promoting (pgp) attributes: nitrogen fixation, production of indole acetic acid like substances, solubilization of phosphate, and ability to antagonize pathogenic fungi. isolates were ranked based on in-vitro-assayed pgp attributes, and 10 superior is ... | 2014 | 24498985 |
| molecular identification of aiia homologous gene from endophytic enterobacter species and in silico analysis of putative tertiary structure of ahl-lactonase. | the aiia homologous gene known to encode ahl- lactonase enzyme which hydrolyze the n-acylhomoserine lactone (ahl) quorum sensing signaling molecules produced by gram negative bacteria. in this study, the degradation of ahl molecules was determined by cell-free lysate of endophytic enterobacter species. the percentage of quorum quenching was confirmed and quantified by hplc method (p<0.0001). amplification and sequence blast analysis showed the presence of aiia homologous gene in endophytic enter ... | 2014 | 24309109 |
| enterobacter cloacae complex: clinical impact and emerging antibiotic resistance. | species of the enterobacter cloacae complex are widely encountered in nature, but they can act as pathogens. the biochemical and molecular studies on e. cloacae have shown genomic heterogeneity, comprising six species: enterobacter cloacae, enterobacter asburiae, enterobacter hormaechei, enterobacter kobei, enterobacter ludwigii and enterobacter nimipressuralis, e. cloacae and e. hormaechei are the most frequently isolated in human clinical specimens. phenotypic identification of all species bel ... | 2012 | 22827309 |
| enterobacter cloacae complex isolates harboring blanmc-a or blaimi-type class a carbapenemase genes on novel chromosomal integrative elements and plasmids. | carbapenem-resistant enterobacter cloacae complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by pcr for seven common carbapenemase gene groups, namely, kpc, ndm, oxa-48, vim, imp, ges, and nmc-a/imi. nineteen of the submitted isolates (1.7%) were found to harbor ambler class a blanmc-a or blaimi-type carbapenemases. all 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprof ... | 2017 | 28223374 |
| whole genome sequence of enterobacter ludwigii type strain en-119t, isolated from clinical specimens. | enterobacter ludwigii strain en-119(t) is the type strain of e. ludwigii, which belongs to the e. cloacae complex (ecc). this strain was first reported and nominated in 2005 and later been found in many hospitals. in this paper, the whole genome sequencing of this strain was carried out. the total genome size of en-119(t) is 4952,770 bp with 4578 coding sequences, 88 trnas and 10 rrnas. the genome sequence of en-119(t) is the first whole genome sequence of e. ludwigii, which will further our und ... | 2015 | 25724532 |
| helicoverpa zea gut-associated bacteria indirectly induce defenses in tomato by triggering a salivary elicitor(s). | insect gut-associated microbes modulating plant defenses have been observed in beetles and piercing-sucking insects, but the role of caterpillar-associated bacteria in regulating plant induced defenses has not been adequately examined. we identified bacteria from the regurgitant of field-collected helicoverpa zea larvae using 16s ribosomal rna (rrna) gene sequencing and matrix-assisted laser desorption/ionization time of flight (maldi-tof) mass spectrometry. a combination of biochemical, molecul ... | 2017 | 28170113 |
| draft genome sequence of enterobacter ludwigii ncr3, a heavy metal-resistant rhizobacterium. | we report here the draft genome of enterobacter ludwigii ncr3, a gram-negative bacterium isolated from the carpobrotus rossii (haw.) schwantes rhizosphere. the analysis of the ~4.8-mb draft genome shows that this strain harbors several genes associated with heavy metal resistance and plant growth-promoting activity, suggesting its potential application in microbe-assisted phytoremediation. | 2016 | 27795247 |
| amelioration of drought tolerance in wheat by the interaction of plant growth-promoting rhizobacteria. | drought stress adversely affects the growth and yield of wheat. the present study was planned to investigate the effect of inoculation of plant-growth promoting rhizobacteria (pgpr) strains ig 3 (klebsiella sp.), ig 10 (enterobacter ludwigii) and ig 15 (flavobacterium sp.) in improving drought tolerance in wheat. these pgpr strains were screened for drought tolerance in nutrient broth supplemented with different concentrations (0-25%) of polyethylene glycol (peg6000). effect of pgpr inoculation ... | 2016 | 27607023 |
| nosocomial outbreak of extended-spectrum β-lactamase-producing enterobacter ludwigii co-harbouring ctx-m-8, shv-12 and tem-15 in a neonatal intensive care unit in venezuela. | enterobacter spp. have emerged as an important group of pathogens linked to outbreaks in neonatal intensive care units (nicus), usually involving strains expressing extended-spectrum β-lactamases (esbls). the aim of this study was to describe the first nosocomial bloodstream infection outbreak caused by enterobacter ludwigii co-harbouring ctx-m-8, shv-12 and tem-15 in a nicu in a venezuelan hospital. initial bacterial identification was achieved by vitek(®)2 system and matrix-assisted laser deso ... | 2016 | 27750157 |
| stability assessment of hydro dispersive nanometric permethrin and its biosafety study towards the beneficial bacterial isolate from paddy rhizome. | nanopesticides such as nanopermethrin can serve as an alternative to conventional pesticides causing eco-toxicity. the nanoformulation of this pyrethroid pesticide was carried out by solvent evaporation of pesticide-loaded microemulsion. the z average for the nanopermethrin dispersion in paddy field water was found to be 169.2 ± 0.75 nm with a polydispersity index of 0.371 that exhibits uniform dispersion. further, the nanopermethrin (np) dispersion exhibited an effective stability in the paddy ... | 2016 | 27672046 |
| characterization of endo-β-mannanase from enterobacter ludwigii my271 and application in pulp industry. | β-mannanases are the second most important enzymes for the hydrolysis of hemicelluloses. an endo-β-mannanase from enterobacter ludwigii my271 was purified at 11.7 ± 0.2-fold to homogeneity with a final recovery of 15.2 ± 0.2 %. using purified β-mannanase protein and sds-page, the molecular mass was found to be 43.16 kda. the optimal ph and temperature of the enzyme was found to be 7.0 and 55 °c, respectively. the β-mannanase activity was stable over a broad ph range of ph 2.0-10.0. in addition, ... | 2017 | 27534412 |
| screening and characterization of cold-active β-galactosidase producing psychrotrophic enterobacter ludwigii from the sediments of arctic fjord. | low-temperature-tolerant microorganisms and their cold-active enzymes could be an innovative and invaluable tool in various industrial applications. in the present study, bacterial isolates from the sediment samples of kongsfjord, norwegian arctic, were screened for β-galactosidase production. among the isolates, ks25, ks85, ks60, and ks92 have shown good potential in β-galactosidase production at 20 °c. 16srrna gene sequence analysis revealed the relatedness of the isolates to enterobacter ludw ... | 2016 | 27188973 |
| culturable endophytic bacterial communities associated with field-grown soybean. | assess the diversity of the culturable endophytic bacterial population associated with transgenic and nontransgenic soybean grown in field trial sites in brazil and characterize them phenotypically and genotypically focusing on characteristics related to plant growth promotion. | 2016 | 26744016 |
| first report of an enterobacter ludwigii isolate coharboring ndm-1 and oxa-48 carbapenemases. | | 2013 | 23877704 |
| production of calcite nanocrystal by a urease-positive strain of enterobacter ludwigii and study of its structure by sem. | the present research aimed at evaluating the effects of urease enzyme and increasing ph on calcite nanocrystal formation. unlike some researches, the results showed that caco3 precipitation is not a general phenomenon among the bacteria and if a bacterium has not this ability, it will not be able to produce calcite even with an increase in ph. all urease-positive bacteria had this ability, while only some urease-negative bacteria were able to produce calcite. production and characterization of n ... | 2013 | 23677144 |
| expression of cspe by a psychrotrophic bacterium enterobacter ludwigii pas1, isolated from indian himalayan soil and in silico protein modelling, prediction of conserved residues and active sites. | proteome analysis of enterobacter ludwigii pas1 provide a powerful set of tool to study the cold shock proteins along with that combination of bioinformatics is useful for interpretation of comparative results from many species. there is a considerable interest in the use of psychrotrophic bacteria for nitrogen fixation, especially at hilly regions, thus better understanding of cold adaptation mechanisms too. the psychrotrophic e. ludwigii pas1 grown at 30 and 4 °c, isolated from himalaya soil w ... | 2013 | 23325033 |