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production physiology and properties of a novel fungal fibrinolytic enzyme.a potent extracellular fibrinolytic enzyme was obtained from cultures of the imperfect fungus fusarium semitectum under certain growth conditions. nitrate addition to cultures increased enzyme production. the enzyme showed a versatile proteolytic activity against several protein substrates including casein, gelatin, haemoglobin, bovine serum albumin, and fibrin from both buffalo and human sources. optimal fibrinolysis occurred at ph values around 7.0. the fibrinolytic activity exhibited marked h ...197610688
acute bovine pulmonary emphysema caused by the fungus fusarium semitectum.acute bovine pulmonary emphysema (abpe) is known to occur throughout the world. the documented causes include 3-methylindole, 4-ipomeanol and perilla ketone. although 4-ipomeanol is a phytoallexin from fusarium solani, this is the first reported incidence of toxicity involving fusarium semitectum. this report describes the poisoning of cattle consuming fusarium semitectum contaminated pink half-runner bean refuse.19883388752
kikuyu poisoning of cattle in new south wales and its relationship to pasture fungi on kikuyu grass (pennisetum clandestinum).in the late summer and autumn of 1980 kikuyu poisoning of cattle was diagnosed on 9 farms in new south wales resulting in 213 deaths among 1370 cattle at risk. clinical, autopsy and histopathological findings are described and are consistent with those previously recorded. kikuyu grass samples from 5 paddocks in which deaths were occurring were examined for the presence of fungi. myrothecium spp were not present. fusarium semitectum, f. moniliforme var subglutinans, penicillium spp and a phoma s ...19873689260
[properties of fusarium semitectum rnaase released by the cells into the nutrient medium]. 19734765681
[substrate specificity of penicillin amidase from fusarium semitectum]. 19714996604
[purification of extracellular rnaase of fusarium semitectum]. 19725086797
the normal mycoflora of commodities from thailand. 2. beans, rice, small grains and other commodities.part of a comprehensive study of fungi occurring in commodities in thailand, this paper reports results from 276 samples of mung beans, rice, sorghum and soybeans as well as other minor crops. samples for major commodities were taken from farmers' stocks, middlemen and retail outlets, while those for minor crops were mostly from retail. each sample was divided into two portions, one being examined in bangkok and the second at north ryde. mycological examinations were carried out by direct platin ...19947811571
the normal mycoflora of commodities from thailand. 1. nuts and oilseeds.a comprehensive study was carried out of the fungi occurring in commodities normally traded in thailand. samples of major commodities were obtained from farmers' stocks and middlemen in major producing areas throughout the country. retail samples were obtained from outlets in and around bankok. samples were divided into two portions, one being examined in bangkok, and the second in sydney. after surface disinfection, fungi were enumerated by direct plating on dichloran rose bengal chloramphenico ...19938110599
isoelectric focusing isozyme profiles and taxonomic distances among fusarium species of the sections arthrosporiella and sporotrichiella.isozymes from 18 isolates representing seven species of the fusarium sections arthrosporiella and sporotrichiella were compared by isoelectric focusing in polyacrylamide gels. of the six enzyme systems tested esterase and malate dehydrogenase showed the largest variation. a numerical analysis of the pi values determined for acid phosphatase, esterase, glucose-6-phosphate dehydrogenase, malate dehydrogenase, phosphoglucose isomerase and phosphoglucomutase resulted in a dendrogam demonstrating the ...19938304009
in-vitro antifungal susceptibility of clinical and environmental fusarium spp. strains.the mics of amphotericin b, miconazole, ketoconazole, flucytosine, itraconazole and fluconazole for 19 isolates of fusarium oxysporum, 16 fusarium solani, seven fusarium verticilliodes, four fusarium proliferatum, four fusarium dimerum, three fusarium equiseti, and one each of the following species: fusarium graminearum, fusarium chlamydosporum, fusarium semitectum, fusarium avenaceum and fusarium subglutinans were determined by a broth microdilution method. thirty-eight of these isolates were o ...19979069536
fungi associated with post-harvest rot of black plum (vitex doniana) in nigeria.the fungi associated with rot of vitex doniana fruits (blackplum) were isolated and identified. aspergillus niger, botryodiplodia theobromae, candida spp. penicillium chrysogenum, rhizopus stolonifer, fusarium pallidoroseum f. oxysporum and mucor mucedo were the primary rot causing fungi in contrast to cladosporium herbarum and mucor circinelloides which were just present as secondary colonizers. the rot fungi penetrated mainly through wounds and bruises on the surface of fruits. mature green fr ...19969208478
mycoflora of paddy and milled rice produced in the region of northeastern argentina and southern paraguay.thirty samples of paddy rice and twenty-five of milled rice were obtained from processing centers located in two northern provinces of argentina and one southern province of paraguay. contaminating fungi were enumerated by direct plating on dichloran rose bengal chloramphenicol agar and oxytetracycline glucose yeast extract agar before and after surface disinfection. all fungi were isolated and identified to the genus level and percentage infection of samples calculated. those belonging to the g ...19979310860
[the correlations between modl fungi. i. effect of various physiological factors on correlations between alternari tenuis and fusarium semitectum]. 195313159236
fusarium incarnatum isolated from black tiger shrimp, penaeus monodon fabricius, with black gill disease cultured in vietnam.fusarium incarnatum was isolated from gill lesions of cultured black tiger shrimp, penaeus monodon, in every crop during 2000-2002 in nghe an province, vietnam. infected shrimps showed typical signs of black gill disease and mortalities about a month prior to harvest. detailed morphological examinations, as well as molecular phylogenic analyses based on partial nucleotide sequences of ribosomal dna, were made on the isolates. an artificial infection of kuruma prawn, penaeus japonicus, using two ...200415357709
[allelopathy of root exudates from two genotypes soybeans on root pathogenic fungi].with biological simulation experiment and chemical analysis, this paper studied the allelopathy of carbohydrates, amino acids and organic acids in the root exudates from two genotypes soybeans (9536 and jilin 30) on the pathogenic fungi of root rot. the results showed that the water soluble carbohydrates in the root exudates from test soybeans significantly promoted the growth of fusarium oxysporium and fusarium semitectum at low concentrations and inhibited their growth at high concentrations, ...200515852974
inhibition of expression of fusarium semitectum and other fungi in immature cottonseed.fusarium semitectum berkeley and ravenel and other nonpathogenic fungi appear to contaminate immature cottonseed but cannot express themselves until immediately after seed maturation. it is hypothesized that catechin prevents fungal outgrowth and that inhibition is released when catechin is polymerized during seed maturation.198216346077
fusarium semitectum, a potential mycopathogen against thrips and mites in chilli, capsicum annuum.in india, chilli (capsicum annuum l.) suffers with a characteristic leaf curl symptoms due to the attack of mite, polyphagotarsonemus latus (banks) (acari: tarsonemidae) and thrips, scirtothrips dorsalis hood (thysanoptera: thripidae) or both. experiments were conducted in the fields of college of agriculture, shimoga, india during kharif (september 2003 to january 2004) and summer (march-june) 2004. after proving its pathogenicity, the potential of the mycopathogen, fusarium semitectum was eval ...200617385513
less-frequent fusarium species of clinical interest: correlation between morphological and molecular identification and antifungal susceptibility.forty-eight fusarium isolates morphologically identified as belonging to seven species of clinical interest (i.e., fusarium chlamydosporum, fusarium dimerum, fusarium incarnatum, fusarium napiforme, fusarium nygamai, fusarium proliferatum, and fusarium sacchari) were characterized molecularly by the analysis of the sequences of the tub region of the beta-tubulin gene. f. chlamydosporum and f. dimerum were the most genetically heterogeneous species. a high degree of correlation between the morpho ...200919321723
production of mannitol by fungi from cotton dust.cotton dust associated with high pulmonary function decrements contains relatively high levels of mannitol. in this study, cotton leaf and bract tissue and dust isolated from cotton leaf tissue were examined by optical microscopy, scanning electron microscopy, and capillary gas chromatography. alternaria alternata, cladosporium herbarum, epicoccum purpurascens, and fusarium pallidoroseum were isolated from cotton leaf dust. the fungal samples, cotton dust, and cotton leaf contained mannitol. thi ...198816347688
novel multilocus sequence typing scheme reveals high genetic diversity of human pathogenic members of the fusarium incarnatum-f. equiseti and f. chlamydosporum species complexes within the united states.species limits within the clinically important fusarium incarnatum-f. equiseti and f. chlamydosporum species complexes (fiesc and fcsc, respectively) were investigated using multilocus dna sequence data. maximum-parsimony and maximum-likelihood analyses of aligned dna sequences from four loci resolved 28 species within the fiesc, within which the species were evenly divided among two clades designated incarnatum and equiseti, and four species within the fcsc. sequence data from a fifth locus, be ...200919828752
development of a liquid chromatography/electrospray tandem mass spectrometry assay for the quantification of apicidin, a novel histone deacetylase inhibitor, in rat serum: application to a pharmacokinetic study.apicidin, a fungal metabolite isolated from fusarium pallidoroseum, is a cyclic tetrapeptide that exhibits potent anti-protozoal and anti-angiogenic activities. although extensive studies have been recently conducted to examine the biological and pharmacological action, no information is available on the quantitative analysis of apicidin. to our knowledge, this study is the first to describe a rapid and sensitive liquid chromatography/tandem mass spectrometry (lc/ms/ms) assay method for the quan ...200515645510
mycoflora and potential for mycotoxin production of freshly harvested black bean from the argentinean main production area.a mycological survey was carried out, for the first time, on black bean samples from the northwestern argentinean province of salta in the 1999 harvest season. ten varieties of black beans were evaluated at three locations. species of the genus alternaria were the most prevalent component of the black bean mycoflora. species of fusarium, sclerotinia, rhizoctonia and acremonium were also recorded. the predominant species of the genera isolated were alternaria alternata, sclerotinia sclerotiorum, ...200415487328
structure-activity relationships of derivatives of fusapyrone, an antifungal metabolite of fusarium semitectum.fusapyrone (1) and deoxyfusapyrone (2) are two 3-substituted-4-hydroxy-6-alkyl-2-pyrones isolated from fusarium semitectum that have considerable antifungal activity against molds. because of their low zootoxicity and selective action they are potentially utilizable along with biocontrol yeasts for control of postharvest crop diseases. seven derivatives of 1 (3 and 5-10) and one derivative of 2 (4) were obtained by chemical modifications of the glycosyl residue, the 2-pyrone ring, the aliphatic ...200415137845
immunological detection of fusarium species in cornmeal.an indirect enzyme-linked immunosorbent assay (elisa) was developed to detect fusarium species in foods. antibodies to proteins extracted from the mycelia of fusarium graminearum and fusarium moniliforme (verticillioides) were produced in new zealand white rabbits. these antibodies detected 13 fusarium species in addition to the producer strains. levels of fusarium semitectum and fusarium tricinctum strains were below the detection threshold. the specificity of the assay was tested against 70 mo ...200312636300
[allelopathic effect of root exudates on pathogenic fungi of root rot in continuous cropping soybean].allelopathic effect of root exudates on pathogenic fungi of root rot in continuous cropping soybean was studied by sand culture, water culture, and indoor culture experiments. the results showed that allelopathic promotion of root exudates on the growth of fusarium semitectum, gliocladium roseum and fusarium oxysporum, especially fusarium semitectum reached significant level or especially significant level in continuous cropping soybean compared with the control. allelopathic promotion of root e ...200212216402
high-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, morus indica cv. k2, by constitutive and inducible expression of tobacco osmotin.osmotin and osmotin-like proteins are stress proteins belonging to the plant pr-5 group of proteins induced in several plant species in response to various types of biotic and abiotic stresses. we report here the overexpression of tobacco osmotin in transgenic mulberry plants under the control of a constitutive promoter (camv 35s) as well as a stress-inducible rd29a promoter. southern analysis of the transgenic plants revealed the stable integration of the introduced genes in the transformants. ...201120549349
genetic and functional diversity among the antagonistic potential fluorescent pseudomonads isolated from tea rhizosphere.twenty-five fluorescent pseudomonads from rhizospheric soil of six tea gardens in four district of upper assam, india were isolated and screened for antagonistic activity against fungal pathogens such as fusarium oxysporum f. sp. raphani (for), fusarium oxysporum f. sp. ciceri (foc), fusarium semitectum (fs), and rhizoctonia solani (rs); and bacterial pathogens-staphylococcus aureus (sa), escherichia coli (ec), and klebsiella pneumoniae (kp). most of the isolates exhibited strong antagonistic ac ...201120689953
canker and wilt of black locust (robinia pseudoacacia l.) caused by fusarium species.from the pathological material of black locust trees showing symptoms of wilting of the foliage or canker of the bark the following fusarium species were isolated: fusarium avenaceum (fr.) sacc., fusarium lateritium nees., fusarium semitectum berk. & rav., fusarium solani (mart.) sacc., fusarium sulphureum schlecht. (syn.: fusarium sambucinum fuckel f. 6 wollenw.) the results of the provocation infections of one-year-old black locust seedlings showed that all of the species--except fusarium sola ...200212109155
characterization of gibberellin producing strains of fusarium moniliforme based on dna polymorphism.the gibberellins are one of the major groups of growth promoting hormones and are secondary metabolites of the fungus fusarium moniliforme (perfect stage: gibberella fujikuroi). sixteen strains of fusarium from different geographical regions and different hosts were analysed for their ability to produce gibberellins (ga) and for genetic relatedness by random amplified polymorphic dna (rapd). range of gibberellin production varied between 28.9 to 600.0 mg g(-1) dry weight of mycelium in different ...200212014478
biological interaction between soil fungi and toxocara canis eggs.fungi from the soil of public areas in la plata, argentina were isolated and evaluated for their biological interaction with toxocara canis eggs in vitro. we isolated and identified two fungal species: fusarium pallidoroseum and mucor hiemalis. each species was co-cultured with t. canis eggs in sterile distilled water. the samples were observed by light and scanning electron microscopy (sem) at days 4, 7 and 14 post-inoculation. under the conditions of our experiments, f. pallidoroseum exhibited ...200211750118
lactones. 6. microbial lactonization of gamma,delta-epoxy esters.the ability of 19 microorganisms to perform the enantioselective lactonization of racemic gamma,delta-epoxy ester 3a and its 7-methyl homolog 3b was checked. it was found that rhodotorula rubra preferentialy transformed both substrates to (-)-enantiomers of trans delta-hydroxy-gamma-lactones with ee 76% for 3a and 24% for 3b. the best efficiency (20-30%) and enantioselectivity (ee 60-100%) of formation of (-)-gamma-hydroxy-delta-lactones 6a and 6b was observed for lactonization by botrytis ciner ...200111370019
identification of the etiological agent for necrotizing scute disease in the texas tortoise.epidermal lamellae (scutes) of the texas tortoise, gopherus berlandieri, from southern texas (usa) were observed to be in various stages of necrosis, ranging from localized whitish blemishes to complete degradation of the external portion of the scute. fusarium semitectum was consistently isolated from slivers of infected scute from tortoises. the fungus was not isolated from tortoises exhibiting no lesions. confocal microscopy confirmed the presence of septate mycelia inside the scutes, and iso ...200111310871
biological characterization of fusapyrone and deoxyfusapyrone, two bioactive secondary metabolites of fusarium semitectum.fusapyrone (1) and deoxyfusapyrone (2), two alpha-pyrones originally isolated from rice cultures of fusarium semitectum, were tested in several biological assays. compounds 1 and 2 showed considerable antifungal activity against several plant pathogenic and/or mycotoxigenic filamentous fungi, although they were inactive toward yeasts isolated from plants and the gram-positive bacterium bacillus megaterium in disk diffusion assays. compound 1 was consistently more active than 2. among the tested ...200010978211
enzymatic degradation of monocrotophos by extracellular fungal op hydrolases.the present study explores the potential of extracellular fungal organophosphate (op) hydrolase for the degradation of monocrotophos. extracellular op hydrolases were isolated and purified from five different fungal isolates viz. aspergillus niger (m1), aspergillus flavus (m2), penicillium aculeatum (m3), fusarium pallidoroseum (m4), and macrophomina sp. (m5) by amso4 precipitation, dialysis, and g-100 chromatography. m3 showed highest percentage yield of 68.81 followed by 55.41 % for m1. each o ...201323963716
high performance liquid chromatography for the analysis of fusapyrone and deoxyfusapyrone, two antifungal alpha-pyrones from fusarium semitectum.a simple, very sensitive and rapid hplc method was developed for the simultaneous quantitative analysis of both fusapyrone (fp) and deoxyfusapyrone (dfp), the two antifungal 3-substituted-4-hydroxy-6-alkyl-2-pyrones isolated from rice culture of fusarium semitectum, in crude extracts. such method was optimized on c-18 reverse phase column, using the isolated metabolites as standards, with a sequence of linear elution steps with a meoh-h(2)o mixture and using an ultraviolet detector fixed at 285 ...199910797640
influence of temperature, water activity and incubation time on fungal growth and production of ochratoxin a and zearalenone by toxigenic aspergillus tubingensis and fusarium incarnatum isolates in sorghum seeds.the major objective of this study was to describe the effect of water activity and temperature on radial growth and production of ochratoxin a (ota) and zearalenone (zea) on sorghum grains of three aspergillus tubingensis and three fusarium incarnatum isolates. the water activity range was 0.91-0.99 aw for f. incarnatum isolates and 0.88-0.99 aw for a. tubingensis isolates. temperatures of incubation were 15, 25 and 37°c for both species. mycotoxin production was determined after 7, 14, 21 and 2 ...201727883966
fusarium species from nepalese rice and production of mycotoxins and gibberellic acid by selected species.infection of cereal grains with fusarium species can cause contamination with mycotoxins that affect human and animal health. to determine the potential for mycotoxin contamination, we isolated fusarium species from samples of rice seeds that were collected in 1997 on farms in the foothills of the nepal himalaya. the predominant fusarium species in surface-disinfested seeds with husks were species of the gibberella fujikuroi complex, including g. fujikuroi mating population a (anamorph, fusarium ...200010698766
fungicide sensitivity among isolates of colletotrichum truncatum and fusarium incarnatum-equiseti species complex infecting bell pepper in trinidad.bell pepper is an economically important crop worldwide; however, production is restricted by a number of fungal diseases that cause significant yield loss. chemical control is the most common approach adopted by growers to manage a number of these diseases. monitoring for the development to resistance to fungicides in pathogenic fungal populations is central to devising integrated pest management strategies. two fungal species, fusarium incarnatum-equiseti species complex (fiesc) and colletotri ...201728381958
survey of termite-inhabited soil and mosquito breeding sites in lucknow, india for potential mycopathogens of anopheles stephensi.during a short survey of soil and mosquito breeding sites in lucknow, india for potential mycopathogen from a period of august-october 1996, 11 species of fungi in 5 genera were isolated using live mosquito larvae as host, aspergillus flavus, a. fumigatus and fusarium semitectum were the most frequently isolated species. other fungi recorded were a. niger, a. ochraceus, a. terreus, a. versicolor, geotrichum candidum, penicillium verrucosum, paecilomyces sp. and fusarium sp. (liseola/elegans comp ...199810481288
fusapyrone and deoxyfusapyrone, two antifungal alpha-pyrones from fusarium semitectum.a strain of fusarium semitectum berk. & rav. from maize stalk rot in southern italy produced bioactive metabolites when cultured on autoclaved rice kernels at room temperature for 4 weeks. the organic extracts of fungal culture showed a strong antibiotic activity towards geotrichum candidum in disk diffusion assays, but they were not toxic to artemia salina larvae. two antifungal metabolites were isolated and characterized by chemical and spectroscopic methods as two 3-substituted-4-hydroxy-6-al ...19948032694
antifungal susceptibility of 44 clinical isolates of fusarium species determined by using a broth microdilution method.the mics and minimum fungicidal concentrations of amphotericin b, natamycin, miconazole, itraconazole, and flucytosine against 17 isolates of fusarium solani, 14 isolates of fusarium moniliforme, 10 isolates of fusarium oxysporum, and 3 isolates of fusarium semitectum were determined by a broth microdilution method. amphotericin b and natamycin were the most active agents tested and failed to show any inoculum size effect. in contrast, miconazole and itraconazole showed poor inhibitory and fungi ...19892817867
decreased immune response and increased incidence of tibial dyschondroplasia caused by fusaria grown on sterile corn.corn, feed, and litter samples reported to be associated with feed refusal, diarrhea, leg weakness, and mortality were evaluated for the presence of toxic substances. intubated residues of ethyl acetate extracts of these samples did not cause gross lesions, diarrhea, or mortality in young new hampshire x single comb white leghorn crossbred chicks. fusarium moniliforme was the predominant fungal species found in unpelleted feed and corn samples. young broiler chicks, fed diets supplemented with 2 ...19912027835
isolation of beauvericin as an insect toxin from fusarium semitectum and fusarium moniliforme var. subglutinans.nonpolar methylene chloride-soluble extracts from the mycelia of fusarium semitectum and fusarium moniliforme var. subglutinans were toxic to colorado potato beetles. the major toxic metabolite was isolated and found to be the cyclodepsipeptide, beauvericin. this is the first report of the isolation of beauvericin from the genus fusarium.19911749401
fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole.silver nanoparticles (ag-nps) are known to have inhibitory and bactericidal effects. resistance of fungal infections has emerged in recent years and is a major health problem. here, we report the extracellular biosynthesis of ag-nps using a common fungus, alternaria alternata. also in this study, these nanoparticles were evaluated for their part in increasing the antifungal activity of fluconazole against phoma glomerata, phoma herbarum, fusarium semitectum, trichoderma sp., and candida albicans ...200919616127
pyrrole and indole alkaloids from an endophytic fusarium incarnatum (hki00504) isolated from the mangrove plant aegiceras corniculatum.two new pyrrole alkaloids, n-[4-(2-formyl-5-hydroxymethyl-pyrrol-1-yl)-butyl]-acetamide (1) and n-[5-(2-formyl-5-hydroxymethyl-pyrrol-1-yl)-pentyl]-acetamide (2), and a new indole derivative (3ar,8ar)-3a-acetoxyl-1,2,3,3a,8,8a-hexahydropyrrolo-[2,3-b]indol (3) were isolated, together with ( - )-3a-hydroxyfuroindoline, (3ar,8as)-1-acetyl-1,3,3a,8,8a-hexahydropyrrolo-[2,3-b]indol-3a-ol, and n-acetyltryptamine a, from an endophytic ascomycetous fungus, fusarium incarnatum (hki00504), which was isol ...200818696331
composition and antifungal activity of the essential oil of the brazilian chenopodium ambrosioides l.the antifungal activity of essential oil (eo) from the brazilian epazote (chenopodium ambrosioides l.) was evaluated by the poison food assay at concentrations of 0.3%, 0.1%, and 0.05% with eight postharvest deteriorating fungi (aspergillus flavus, aspergillus glaucus, aspergillus niger, aspergillus ochraceous, colletotrichum gloesporioides, colletotrichum musae, fusarium oxysporum, and fusarium semitectum). eo components were tentatively identified by kováts retention indices (ris) using gas ch ...200818679750
efficacy of female culex quinquefasciatus with entomopathogenic fungus fusarium pallidoroseum.this study was conducted to isolate and identify natural entomopathogenic fungi from female culex quinquefasciatus and to test their adulticidal activity. field-collected female c. quinquefasciatus died early and were placed on a saboraud's dextrose agar plates for growth and isolation of natural entomopathogenic fungi. the plates were maintained in an incubator at 24+/-2 degrees c for 3 days. four fungal species were isolated in two genera namely, aspergillus and fusarium. the identified fungal ...200818327611
trichothecenes and mycoflora in wheat harvested in nine locations in buenos aires province, argentina.a total of 120 freshly harvested wheat samples from the 2004 season in nine locations from northern buenos aires province, argentina, were analysed for trichothecene natural occurrence and associated mycoflora, and for determining the influence of commonly used fungicide field treatment and the cultivar type on trichothecene contamination. the trichothecenes t-2 tetraol, t-2 triol, ht-2 and t-2 toxin (ht-2, t-2), diacetoxyscirpenol (das), nivalenol (niv), deoxynivalenol (don), 3-acetyldeoxynival ...200818266077
phylogeny and haplotype analysis of fungi within the fusarium incarnatum-equiseti species complex.fusarium spp. are ranked among the top 10 most economically and scientifically important plant-pathogenic fungi in the world and are associated with plant diseases that include fruit decay of a number of crops. fusarium isolates infecting bell pepper in trinidad were identified based on sequence comparisons of the translation elongation factor gene (ef-1a) with sequences of fusarium incarnatum-equiseti species complex (fiesc) verified in the fusarium-id database. eighty-two isolates were identif ...201727901448
pathogenicity of fusarium semitectum against crop pests and its biosafety to non-target organisms.microbial control is receiving more attention, since these alternative tactics, compared to chemical control methods, are energy saving, non polluting, ecologically sound and sustainable. a mycopathogen, fusarium semitectum berk. and rav. (arsef 7233) was isolated from diseased cadavers of aphid (aphis gossypii) and cultured in saboraud maltose agar supplemented with yeast extract medium (smay). being isolated first time from the chilli ecosystem its potential was evaluated. experiments were con ...200617385514
the medicago truncatula n5 gene encoding a root-specific lipid transfer protein is required for the symbiotic interaction with sinorhizobium meliloti.the medicago truncatula n5 gene is induced in roots after sinorhizobium meliloti infection and it codes for a putative lipid transfer protein (ltp), a family of plant small proteins capable of binding and transferring lipids between membranes in vitro. various biological roles for plant ltp in vivo have been proposed, including defense against pathogens and modulation of plant development. the aim of this study was to shed light on the role of mtn5 in the symbiotic interaction between m. truncat ...200919888823
optimization of media components for laccase production by litter dwelling fungal isolate fusarium incarnatum ld-3.laccase production by solid state fermentation (ssf) using an indigenously isolated litter dwelling fungus fusarium incarnatum ld-3 was optimized. fourteen medium components were screened by the initial screening method of plackett-burman. each of the components was screened on the basis of 'p' (probability value) which was above 95% confidence level. ortho-dianisidine, thiamine hcl and cuso(4) . 5 h(2)o were identified as significant components for laccase production. the central composite desi ...201020082375
antifungal activities of ethanolic extract from jatropha curcas seed cake.phorbol ester extraction was carried out from jatropha curcas seed cake, a by-product from the bio-diesel fuel industry. four repeated extractions from 5 g j. curcas seed cake using 15 ml of 90% (v/v) ethanol and a shaking speed of 150 rev/min gave the highest yield of phosbol esters. the ethanolic extract of j. curcas seed cake showed antifungal activities against important phytofungal pathogens: fusarium oxysporum, pythium aphanidermatum, lasiodiplodia theobromae, curvularia lunata, fusarium s ...201020208435
bacillus-based biocontrol of fusarium disease on tomato cultures in burundi.the biocontrol potential of a plant growth-promoting rhizobacterium (pgpr) bacillus subtilis s499 on tomato was studied in open field sites in low attitude area of the plain of imbo in burundi. application of the pgpr strain on seed before sowing have significantly increased growth and fruit yield of tomato plants in addition to its remarkable control of a local important disease caused by a fungus type-fusarium.. this pathogen causing large tosses in burundi tomato plantings is closely related ...200920222546
functional characterization and manipulation of the apicidin biosynthetic pathway in fusarium semitectum.apicidin is a cyclic tetrapeptide produced by certain isolates of fusarium semitectum and has been shown to inhibit apicomplexan histone deacetylase. an apicidin-producing strain (kctc16676) of the filamentous fungus was mutated using an agrobacterium tumefaciens-mediated transformation, resulting in 24 apicidin-deficient mutants. three of the mutants had a t-dna insertion in a gene that encodes a non-ribosomal peptide synthetase (nrps). results of sequence, expression, and gene deletion analyse ...201020233305
phylogenetic diversity of fusarium incarnatum-equiseti species complex isolated from spanish wheat.wheat is the most important cereal grown in the european union and spain is its fifth largest wheat producer. there is little information about fusarium species associated with wheat in spain. phylogenetic diversity of 51 strains belonging to fusarium incarnatum-equiseti species complex (fiesc) isolated from spanish wheat was investigated using partial sequences of the translation elongation factor gene (ef-1α). maximum-parsimony and bayesian analysis of aligned dna sequences resolved 18 haploty ...201424890361
[Identification of soil-borne fungi using Fourier transform infrared spectroscopy].Fourier transform infrared (FTIR) attenuated total reflectance (ATR) spectroscopy was used in combination with multivariate statistic analysis for identification of soil-borne fungi that causes severe economic damage to agriculture: Fusarium monili forme, Fusarium semitectum, Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Sclerotinia sclerotiorum, Pythium aphanidermatum and Phytophthora capsici. The original FTIR spectra were normalized, and the second derivatives were calculated, from ...201122007392
mycobiota variation in stored rice straw and its cellulolytic profile.rice straw (rs) one of most important agrowaste worldwide. variation in mycobiota inhabiting long stored rs and its cellulolytic profile were studied. the highest number of fungi (23 species) was recovered from 1st storage period (1-3 year). alternaria alternata, aspergillus sp., cladosporium herbarum, fusarium incarnatum, geotrichum candidum, penicillium sp., stemphylium lycopersici and ulocladium atrum are the most frequent genera. among 21 fungal species recovered in the 2nd period (3-5 year) ...201426031023
tetramic acid analogues produced by coculture of saccharopolyspora erythraea with fusarium pallidoroseum.coculture of the fungus fusarium pallidoroseum with the bacterium saccharopolyspora erythraea was found to produce three new decalin-type tetramic acid analogues related to equisetin. the structures were determined by spectroscopic methods. the absolute configurations were established by circular dichroism spectroscopy and comparing the data with those of equisetin.201424422636
halotolerant ability and α-amylase activity of some saltwater fungal isolates.four halotolerant fungal isolates originating from the saltwater lake urmia in iran were selected during a screening program for salt resistance and α-amylase activity. the isolates were identified based on sequencing the its region and a part of the β-tubulin gene, as penicillium chrysogenum (isolate u1; cbs 132820), fusarium incarnatum (isolate u2; cbs 132821), and penicillium polonicum (isolate u3; cbs 132822, and isolate u4; cbs 132823). the growth of these isolates was determined by measuri ...201324250679
application of a multiplex suspension array for rapid and simultaneous identification of clinically important mold pathogens.we have developed a microsphere-based suspension array (msa) for the identification of 23 medically important mold pathogens including aspergillus spp., fusarium spp., mucor spp., rhizopus spp., rhizomucor pusillus, penicillium marneffei, saksenaea vasiformis, apophysomyces elegans, lichtheimia corymbifer, and syncephalastrum racemosum. twenty-one oligonucleotide probes were designed based on the internal transcribed spacer (its2) region for species level identification of molds. among the 21 pr ...201222771862
an impedance-based mold sensor with on-chip optical reference.a new miniaturized sensor system with an internal optical reference for the detection of mold growth is presented. the sensor chip comprises a reaction chamber provided with a culture medium that promotes the growth of mold species from mold spores. the mold detection is performed by measuring impedance changes with integrated electrodes fabricated inside the reaction chamber. the impedance change in the culture medium is caused by shifts in the ph (i.e., from 5.5 to 8) as the mold grows. in ord ...201627690039
effect of an organophosphate pesticide, monocrotophos, on phosphate-solubilizing efficiency of soil fungal isolates.soil is a sink of pesticide residues as well as microorganisms. fungi are well known for solubilization of inorganic phosphates, and this activity of fungal isolates may be affected by the presence of pesticide residues in the soil. in the present study, five generically different fungal isolates, viz. aspergillus niger jq660373, aspergillus flavus, penicillium aculeatum jq660374, fusarium pallidoroseum and macrophomina sp., were tested and compared for their phosphate-solubilizing ability in th ...201525344433
comparative purification and characterization of two distinct extracellular monocrotophos hydrolases secreted by penicillium aculeatum and fusarium pallidoroseum isolated from agricultural fields.the present study aimed at a comparative characterization of two distinct extracellular monocrotophos hydrolases, from penicillium aculeatum itcc 7980.10 (m3) and fusarium pallidoroseum itcc 7785.10 (m4), isolated from agricultural fields. the mcp hydrolases were purified by sephadex g-100 column and deae-sepharose cl-6b ion-exchange column followed by sds-page analysis, which showed the presence of two hydrolases, of 33 and 67 kda respectively. both enzymes were most active at alkaline ph and w ...201323666511
in vitro comparative analysis of monocrotophos degrading potential of aspergillus flavus, fusarium pallidoroseum and macrophomina sp.fungal degradation is emerging as a new powerful tool for the removal of potent neurotoxin pesticide, monocrotophos. therefore, the present study is aimed at comparative characterization of monocrotophos degrading ability of three different fungal strains. fungal strains were isolated from local agricultural soil by enrichment culture method, screened by gradient culture and identified as aspergillus flavus, fusarium pallidoroseum and macrophomina sp. growth kinetics revealed a direct positive i ...201424179090
incidence of mycoflora and mycotoxins in some edible fruits and seeds of forest origin.twelve fungi namelyalternaria alternata, aspergillus flavus, a niger, a ochraceus, actinomucor repens, capnodoium spp., curvularia lunata, fusarium pallidoroseum, f solani, f verticillioides, penicillium citrinum and rhizopus stolonifer were recorded from samples ofaegle marmelos, aesculus indica, buchanania lanzan andpinus gerardiana. in case ofprunus amygdalus only rstolonifer was recorded. a significant variation in pattern of mycoflora incidence was observed in terms of source and season. fu ...200123605675
biosynthesis of archetypal plant self-defensive oxylipins by an endophytic fungus residing in mangrove embryos.a tree's travel companion: a fungal endophyte (fusarium incarnatum) isolated from a viviparous propagule (embryo) of a mangrove tree produces typical plant defense oxylipins. stable-isotope labeling experiments revealed that the endophyte biosynthesizes coriolic acid, didehydrocoriolic acid, and an epoxy fatty acid derived from linoleic acid by a process involving δ(15)-desaturation and 13-lipoxygenation.201223165938
cytotoxic alkaloids from fusarium incarnatum associated with the mangrove tree aegiceras corniculatum.several unusual alkaloids, n-2-methylpropyl-2-methylbutenamide (1), 2-acetyl-1,2,3,4-tetrahydro-β-carboline (2), fusarine (3), fusamine (4), and 3-(1-aminoethylidene)-6-methyl-2h-pyran-2,4(3h)-dione (5), were isolated from the culture broth of fusarium incarnatum (hki0504), an endophytic fungus of the mangrove plant aegiceras corniculatum. compounds 2, 4, and 5 exhibit weak antiproliferative and cytotoxic activities against huvec, k-562, and hela human cell lines, respectively.201222439674
in vivo confocal microscopy for the detection of canine fungal keratitis and monitoring of therapeutic response.to describe in vivo corneal confocal microscopy of dogs during the clinical course of fungal keratitis and correlate findings with clinical evaluations and an ex vivo experimental canine fungal keratitis model.201626061232
fusarium species isolated from pennisetum clandestinum collected during outbreaks of kikuyu poisoning in cattle in south africa.kikuyu poisoning occurs sporadically in south africa. it is of major economic importance, as valuable dairy cows are often poisoned by it, and once affected, the mortality rate is high. pennisetum clandestinum samples were collected during eight outbreaks of kikuyu poisoning in cattle in the eastern cape province of south africa from 2008 to 2010. the kikuyu grass samples were submitted specifically for the isolation and molecular identification of fusarium species, as it was recently suggested ...201425686222
<i>fusarium</i> species isolated from <i>pennisetum clandestinum</i> collected during outbreaks of kikuyu poisoning in cattle in south africa.kikuyu poisoning occurs sporadically in south africa. it is of major economic importance, as valuable dairy cows are often poisoned by it, and once affected, the mortality rate is high. pennisetum clandestinum samples were collected during eight outbreaks of kikuyu poisoning in cattle in the eastern cape province of south africa from 2008 to 2010. the kikuyu grass samples were submitted specifically for the isolation and molecular identification of fusarium species, as it was recently suggested ...201428235286
pathogenic ability and saline stress tolerance of two fusarium isolates from odontesthes bonariensis eggs.several fungal species represent a potential risk to embryos of odontesthes bonariensis (cuvier and valenciennes, 1835), a euryhaline freshwater fish that lives in the pampean inland waters and has potential economic relevance.201626674586
genus-specific primers for study of fusarium communities in field samples.fusarium is a large and diverse genus of fungi of great agricultural and economic importance, containing many plant pathogens and mycotoxin producers. to date, high-throughput sequencing of fusarium communities has been limited by the lack of genus-specific primers targeting regions with high discriminatory power at the species level. in the present study, we evaluated two fusarium-specific primer pairs targeting translation elongation factor 1 (tef1). we also present the new primer pair fa+7/ra ...201526519387
molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran.fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ...201123961146
fusarium incarnatum/equiseti hemodialysis graft infection.hemodialysis graft infections typically occur as a result of contamination by skin flora at the time of insertion or become secondarily infected after high-grade bacteremia. infection of implanted vascular devices with filamentous fungi is rare. we report a case of infection of an implanted polytetrafluoroethylene dialysis graft with fusarium incarnatum/equiseti that did not grow in cultures of tissue but was identified by molecular means.201024470894
veterinary fusarioses within the united states.multilocus dna sequence data were used to assess the genetic diversity and evolutionary relationships of 67 fusarium strains from veterinary sources, most of which were from the united states. molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. the majority of the veterinary isolates (47/67 = 70.1%) were nested within the fusarium solani sp ...201627605713
spectrum of fusarium infections in tropical dermatology evidenced by multilocus sequencing typing diagnostics.fusarium species are emerging causative agents of superficial, cutaneous and systemic human infections. in a study of the prevalence and genetic diversity of 464 fungal isolates from a dermatological ward in thailand, 44 strains (9.5%) proved to belong to the genus fusarium. species identification was based on sequencing a portion of translation elongation factor 1-alpha (tef1-α), rdna internal transcribed spacer and rna-dependent polymerase subunit ii (rpb2). our results revealed that 37 isolat ...201525530264
a polyphasic approach for characterization of a collection of cereal isolates of the fusarium incarnatum-equiseti species complex.dna-based phylogenetic analyses have resolved the fungal genus fusarium into multiple species complexes. the f. incarnatum-equiseti species complex (fiesc) includes fusaria associated with several diseases of agriculturally important crops, including cereals. although members of fiesc are considered to be only moderately aggressive, they are able to produce a diversity of mycotoxins, including trichothecenes, which can accumulate to harmful levels in cereals. high levels of cryptic speciation ha ...201627376677
formation ratios of zearalanone, zearalenols, and zearalanols versus zearalenone during incubation of fusarium semitectum on sorghum and ratios in naturally contaminated sorghum.we incubated fusarium semitectum on sorghum and measured the production of zearalenone (zen) and zen-related compounds (zearalanone (zan), α-zearalenol (α-zel), β-zearalenol (β-zel), α-zearalanol (α-zal) and β-zearalanol (β-zal)) in the culture by lc-ms. of the five zen-related compounds, zan and β-zel were mainly detected. the concentrations of zen and the five zen-related compounds increased until 9 days after incubation and then increased slightly or stayed constant between days 9 and 15. the ...201526699272
environmental and pathogenic factors inducing brown apical necrosis on fruit of english (persian) walnut.brown apical necrosis (ban) is a most recently described disease affecting english (persian) walnut fruit. ban was only recorded in intensively managed walnut orchards and was found to be a disease complex mainly caused by fusarium species. all fungi associated with this disease are polyphagous and ubiquitous, not specific to walnut. consequently, ban occurrence is more strictly dependent, than generally, on the interaction between pathological features and environmental conditions. environmenta ...201526214123
inhibitory effects of antimicrobial agents against fusarium species.we investigated the inhibitory effects of antibacterial, biocidal, and antifungal agents against fusarium spp. seven fusarium spp: four f. falciforme (fusarium solani species complex), one fusarium spp, one fusarium spp. (fusarium incarnatum-equiseti species complex), and one f. napiforme (gibberella fujikuroi species complex), isolated from eyes with fungal keratitis were used in this study. their susceptibility to antibacterial agents: flomoxef, imipenem, gatifloxacin, levofloxacin, moxifloxac ...201525841054
synthesis and antifungal activities of novel polyheterocyclic spirooxindole derivatives.a series of spirooxindole tetrahydrofuran derivatives 3 were obtained in moderate to good yields via oxindole derivatives 1 and β-arylacrylonitrile derivatives 2via base-mediated cascade [3 + 2] double michael reactions under mild conditions and the application of this method in the synthesis of bioactive analogues, such as functionalized spirooxindole octahydrofuro[3,4-c]pyridine derivatives 4 which contain two new heterocyclic rings and two quaternary carbon centers, has also been developed. s ...201525820179
pathogenicity of three entomopathogenic fungi to matsucoccus matsumurae.matsucoccus matsumurae (kuwana) (hemiptera: coccoidea: matsucoccidae) is an invasive alien species and a destructive pest of two native chinese pines, pinus tabulaeformis carr. and p. massoniana lamb., throughout the eastern regions of china. the pathogenicity of three entomopathogenic fungi, lecanicillium lecanii strain v3.4504 and v3.4505, fusarium incarnatum-equiseti strain heb01 and lecanicillium fungicola strain heb02, against m. matsumurae was tested in four instars, to evaluate their pote ...201425068397
isolation of the tetrapeptide apicidins g, h and i from the fungus fusarium semitectum.this study reports the isolation and characterization of three new tetrapeptides, apicidins g (1), h (2) and i (3), along with the known apicidin (4), apicidin a (5), apicidin c (6), diketopiperazine 7, equisetin (8) and 7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone (9). the structures of the new compounds were deduced by 2d nmr spectroscopic and msdata.201424689299
fungal endophthalmitis associated with compounded products.fungal endophthalmitis is a rare but serious infection. in march 2012, several cases of probable and laboratory-confirmed fungal endophthalmitis occurring after invasive ocular procedures were reported nationwide. we identified 47 cases in 9 states: 21 patients had been exposed to the intraocular dye brilliant blue g (bbg) during retinal surgery, and the other 26 had received an intravitreal injection containing triamcinolone acetonide. both drugs were produced by franck's compounding lab (ocala ...201424447640
structure elucidation and antimalarial activity of apicidin f: an apicidin-like compound produced by fusarium fujikuroi.apicidins are cyclic tetrapeptides with histone deacetylase inhibitory activity. since their discovery in 1996 a multitude of studies concerning the activity against protozoa and certain cancer cell lines of natural and synthetic apicidin analogues have been published. until now, the only published natural sources of apicidin are the fungus fusarium pallidoroseum, later known as f. semitectum and two unspecified fusarium strains. the biosynthetic origin of apicidins could be associated with a ge ...201324195442
genome sequences of six wheat-infecting fusarium species isolates.fusarium pathogens represent a major constraint to wheat and barley production worldwide. to facilitate future comparative studies of fusarium species that are pathogenic to wheat, the genome sequences of four fusarium pseudograminearum isolates, a single fusarium acuminatum isolate, and an organism from the fusarium incarnatum-f. equiseti species complex are reported.201324009115
[effect of sodium chloride concentration in the medium on the composition of the membrane lipids and carbohydrates in the cytosol of the fungus fusarium sp].the fungus fusarium sp. isolated from saline soil was identified by the its1-5.8s-its2 and the d1/d2 domains of lsu rna as a member of the fusarium incarnatum-equiseti species group. its growth patterns on media with different nacl concentrations indicated its adaptation as halotolerance. the mechanisms of halotolerance included accumulation of arabitol a five-atom noncyclic polyol, a decreased sterols/phospholipids ratio, elevated level of phosphatidic acids in the phospholipids, and increased ...201525509398
possible role of laccase from fusarium incarnatum uc-14 in bioremediation of bisphenol a using reverse micelles system.bisphenol a [2,2 bis (4 hydroxyphenyl) propane] is widely used in the variety of industrial and residential applications such as the synthesis of polymers including polycarbonates, epoxy resins, phenol resins, polyesters and polyacrylates. bpa has been recognized as an endocrine disrupting chemicals (edc), thus it is necessary to assess its biodegradability or fate in the natural environment. in general, environmental pollutant such as bpa does not dissolve in aqueous media, owing to their high ...201323611799
molecular analyses of fusarium isolates recovered from a cluster of invasive mold infections in a brazilian hospital.invasive fusariosis (if) is a rare but often fatal fungal infection in immunosuppressed patients. in 2007, cases of if above the expected epidemiologic baseline were detected in the hematology ward of a hospital in rio de janeiro, brazil. possible sources of infection were investigated by performing environmental sampling and patient isolate collection, followed by molecular typing. isolates from dermatology patients with superficial fusariosis were included in the study for comparison to molecu ...201323363475
rapid identification of fusarium graminearum species complex using rolling circle amplification (rca).rolling circle amplification (rca) of dna is a sensitive and cost effective method for the rapid identification of pathogenic fungi without the need for sequencing. amplification products can be visualized on 1% agarose gel to verify the specificity of probe-template binding or directly by adding fluorescent dyes. fusarium head blight (fhb) is currently the world's largest threat to the production of cereal crops with the production of a range of mycotoxins as an additional risk. we designed set ...201222326479
biosynthesis and stabilization of au and au-ag alloy nanoparticles by fungus, fusarium semitectum.crystallized and spherical-shaped au and au-ag alloy nanoparticles have been synthesized and stabilized using a fungus, f. semitectum in an aqueous system. aqueous solutions of chloroaurate ions for au and chloroaurate and ag(+) ions (1 : 1 ratio) for au-ag alloy were treated with an extracellular filtrate of f. semitectum biomass for the formation of au nanoparticles (aunp) and au-ag alloy nanoparticles (au-agnp). analysis of the feasibility of the biosynthesized nanoparticles and core-shell al ...200827878009
occurrence of root rot and vascular wilt diseases in roselle (hibiscus sabdariffa l.) in upper egypt.roselle (hibiscus sabdariffa l.) family malvaceae is an important crop used in food, cosmetics and pharmaceutics industries. roselle is cultivated mainly in upper egypt (qena and aswan governorates) producing 94% of total production. root rot disease of roselle is one of the most important diseases that attack both seedlings and adult plants causing serious losses in crop productivity and quality. the main objective of the present study is to identify and characterize pathogens associated with r ...201424808737
trichothecenes and zearalenone production by fusarium equiseti and fusarium semitectum species isolated from argentinean soybean.fusarium equiseti and fusarium semitectum represent the most abundant species in the fusarium complex isolated from flowers, soybean pods and seeds in argentina. the aim of the present study was to assess the production of major type a and type b trichothecenes (diacetoxyscirpenol, neosolaniol, t-2 toxin and ht-2 toxin, nivalenol, deoxynivalenol) and zearalenone by 40 f. equiseti and 22 f. semitectum isolates on rice culture. mycotoxins were determined by hplc with fluorescence detection after d ...201222830612
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