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revisited its2 phylogeny of anopheles (anopheles) hyrcanus group mosquitoes: reexamination of unidentified and misidentified its2 sequences.the anopheles (anopheles) hyrcanus group of mosquitoes is very important for human health because some are malarial vector mosquitoes. despite their pathological importance, some unidentified internal transcribed spacer 2 (its2) sequences have been reported from members of this group, and their phylogenetic relationships have been rarely understood. in the present study, 84 its2 sequences for the hyrcanus group members were retrieved from genbank. the detailed sequence comparison unambiguously r ...200717514381
anopheles kleini, anopheles pullus, and anopheles sinensis: potential vectors of plasmodium vivax in the republic of korea.anopheles sinensis wiedemann (63.3%) was the most abundant anopheles mosquito captured at cowshed resting collections in malaria high-risk areas (northern gyeonggi province) near the demilitarized zone (dmz) in korea during 2005, followed by anopheles kleini rueda (24.7%) and anopheles pullus m. yamada (8.7%). at cowshed resting collections in malaria low-risk areas (jeonnam and gyeongnam provinces), an. sinensis accounted for 96.8% of all anopheles spp. collected, followed by an. kleini rueda ( ...200718047210
a locally acquired falciparum malaria via nosocomial transmission in korea.a 57-year old man who was admitted to an emergency room of a tertiary hospital with hemoptysis developed malarial fever 19 days later and then died from severe falciparum malaria 2 days later. he had not traveled outside of korea for over 30 years. through intensive interviews and epidemiological surveys, we found that a foreign patient with a recent history of travel to africa was transferred to the same hospital with severe falciparum malaria. we confirmed through molecular genotyping of the m ...200919724701
challenges with using names to link digital biodiversity information. 201627346955
evidence to support natural hybridization between anopheles sinensis and anopheles kleini (diptera: culicidae): possibly a significant mechanism for gene introgression in sympatric populations.malaria caused by plasmodium vivax is still a public health problem in the republic of korea (rok), particularly regarding the recent re-emergence of this malarial species near the demilitarized zone in northwestern paju city, gyeonggi-do province. currently, at least 4 species (an. kleini, an. pullus, an. belenrae and an. lesteri) of the hyrcanus group are reported as possible natural vectors of vivax malaria in the rok, and an. sinensis, which is the most dominant species, has long been incrim ...201424443885
distribution of malaria vectors and incidence of vivax malaria at korean army installations near the demilitarized zone, republic of korea.as a result of the reintroduction of malaria in the republic of korea (rok) in 1993 and the threat to military and civilian populations, the korea military national defense (mnd) increased emphasis on vector control in 2012 at rok army (roka) installations located near the dmz, while decreasing chemoprophylaxis, fearing potential drug resistance. mosquito surveillance demonstrated a need for continuous monitoring of disease patterns among roka soldiers and vector malaria infection rates to ensur ...201627150110
genetic compatibility between anopheles lesteri from korea and anopheles paraliae from thailand.to assess differentiation and relationships between anopheles lesteri and anopheles paraliae we established three and five iso-female lines of an. lesteri from korea and an. paraliae from thailand, respectively. these isolines were used to investigate the genetic relationships between the two taxa by crossing experiments and by comparing dna sequences of ribosomal dna second internal transcribed spacer (its2) and mitochondrial dna cytochrome c oxidase subunit i (coi) and subunit ii (coii). resul ...023778670
Preliminary vivax malaria vector competence for three members of the Anopheles hyrcanus group in the Republic of Korea.In a study on the comparative susceptibility of Anopheles kleini, An. lesteri, and An. sinensis to Plasmodium vivax, we examined the feeding of laboratory-reared mosquitoes on blood of a patient carrying gametocytes from the Republic of Korea. Sporozoites in salivary glands from day 14 postfeeding were detected in An. kleini and An. lesteri with numbers high enough to initiate infection, while no sporozoites were detected in the salivary glands of An. sinensis. This result suggests that An. klei ...201122017097
Mosquito species composition and Plasmodium vivax infection rates on Baengnyeong-do (island), Republic of Korea.Vivax malaria is a significant military and civilian health threat in the north of the Republic of Korea (ROK). The island of Baengnyeong-do is the westernmost point of the ROK and is located close to the southwestern coast of the Democratic People's Republic of Korea (DPRK). Mosquitoes were collected using a black light trap on Baengnyeong-do, and Anopheles spp. were assayed by PCR, to identify the species, and screened for sporozoites of Plasmodium vivax. Of a subsample of 257 mosquitoes, Anop ...201122072836
detection of an antibody against plasmodium vivax in residents of gimpo-si, south korea, using an indirect fluorescent antibody test.first reemerged malaria case was reported in 1993 after two decades absent in south korea. thereafter, plasmodium vivax spreads out near demilitarized zone (dmz). this study investigated the prevalence of p. vivax after the malaria transmission season in gimpo-si where adjacent to dmz of south korea. an indirect fluorescent antibody test (ifat) was performed to evaluate anti-malaria antibodies in blood samples.201121281481
population dynamics of five anopheles species of the hyrcanus group in northern gyeonggi-do, korea.to investigate the population densities of potential malaria vectors, anopheles species were collected by light traps in malaria endemic areas, paju and gimpo, gyeonggi-do of korea. five anopheles hyrcanus sibling species (an. sinensis, an. pullus, an. lesteri, an. kleini, and an. belenrae) were identified by pcr. the predominant species, an. pullus was collected during the late spring and mid-summer, while higher population consists of an. sinensis were collected from late summer to early autum ...201021234242
the susceptibility of anopheles lesteri to infection with korean strain of plasmodium vivax.following its recent re-emergence, malaria has gained renewed attention as a serious infectious disease in korea. three species of the hyrcanusgroup, anopheles lesteri, anopheles sinensis and anopheles pullus, have long been suspected malaria vectors. however, opinions about their vector ability are controversial. the present study was designed with the aim of determining the susceptibility of these mosquitoes to a korean isolate of plasmodium vivax. also, an. sinensis is primarily suspected to ...200919284634
is genetic continuity between anopheles sinensis (diptera: culicidae) and its sibling species due to gene introgression or incomplete speciation?the anopheles mosquito hyrcanus group is widely distributed geographically across both palearctic and oriental regions and comprises 26 valid species. although the species anopheles sinensis wiedemann (1828) is the most common in china and has a low potential vector rank, it has nevertheless long been thought to be an important natural malaria vector within the middle and lower reaches of the yangtze river. a number of previous research studies have found evidence to support the occurrence of na ...201931066895
short report: natural hybrid between anopheles kleini and anopheles sinensis.while analyzing wild mosquitoes caught in paju, south korea, we identified one egg-laying hybrid female between anopheles kleini and anopheles sinensis. additional evidence was obtained by identifying several f(1) progeny and conducting self-crossing between them. sequencing of mitochondrial cytochrome c oxidase subunit 1 sequence suggested that the maternal origin of the female should be an. sinensis . additionally, observation of only two distinct genotypes of f(1) progeny (double band, hybrid ...200919996431
population genetic structure of the malaria vector anopheles sinensis (diptera: culicidae) sensu stricto and evidence for possible introgression in the republic of korea.anopheles sinensis wiedemann sensu stricto (s.s.) is a dominant mosquito and considered a secondary malaria vector in the republic of korea (rok). despite the potential significance for malaria control, population genetics studies have been conducted using only mitochondrial dna (mtdna), and studies of the genetics of hybridization have never been attempted. in this study, 346 specimens from 23 localities were subject to experiments. among them, 305 an. sinensis s.s. specimens from 20 localities ...201526336253
distribution and larval habitats of anopheles species in northern gyeonggi province, republic of korea.a total of 180 larval collection sites (e.g., rice paddies, marshes, ground pools, ponds, stream margins, and irrigation and drainage ditches) was surveyed within a 2 km radius from warrior base training area, 5 km south of panmunjeom (joint security area, demilitarized zone), gyeonggi province, republic of korea (rok), from may through october, 2007 to characterize larval habitat distributions of members of the anopheles hyrcanus group (an. sinensis, an. lesteri, an. pullus, an. belenrae, an. k ...201121635650
colonization and maintenance of anopheles kleini and anopheles sinensis from the republic of korea.colonization of members of the anopheles hyrcanus group is required to facilitate a better understanding of their roles in the epidemiology, vector competence, transmission potential, and maintenance of vivax malaria in the republic of korea (rok). a total of 158 resting blood-fed wild caught anopheles species were collected on august 26, 2011 at tongilchon, near the demilitarized zone, in the rok and shipped on august 28, 2011 to the entomology department, armed forces research institute of med ...201424772671
the relationship between wing length, blood meal volume, and fecundity for seven colonies of anopheles species housed at the armed forces research institute of medical sciences, bangkok, thailand.established colonies of anopheles campestris, anopheles cracens, anopheles dirus, anopheles kleini, anopheles minimus, anopheles sawadwongporni, and anopheles sinensis are maintained at the armed forces research institute of medical sciences (afrims). females were provided blood meals on human blood containing citrate as an anticoagulant using an artificial membrane feeder. the mean wing length, used as an estimate of body size, for each species was compared to blood-feeding duration (time), blo ...201526433074
vector competence of anopheles kleini and anopheles sinensis (diptera: culicidae) from the republic of korea to vivax malaria-infected blood from patients from thailand.in total, 1,300 each of anopheles kleini rueda and anopheles sinensis wiedemann sensu stricto (s.s.) females (colonized from the republic of korea) and anopheles dirus peyton & harrison (thai strain) were allowed to feed on blood from thai malaria patients naturally infected with plasmodium vivax the overall oocyst infection rates for an. dirus, an. kleini, and an. sinensis s.s. were 77.4, 46.1, and 45.9%, respectively. the mean number of oocysts was significantly higher for an. dirus (82.7) com ...201627493248
susceptibility of five species members of the korean hyrcanus group to brugia malayi, and hybridization between b. malayi-susceptible and -refractory anopheles sinensis strains.five species members of the korean hyrcanus group: anopheles pullus, anopheles sinensis, anopheles kleini, anopheles belenrae, and anopheles lesteri were tested for susceptibility to brugia malayi. they were allowed to feed artificially on blood containing b. malayi microfilariae and dissected 14 days after feeding. the susceptibility rates were 60%, 65%, 90%, 100% and 100% in an. pullus, an. sinensis, an. kleini, an. belenrae, and an. lesteri, respectively. as determined by levels of susceptibi ...201424974643
estimating the malaria transmission of plasmodium vivax based on serodiagnosis.plasmodium vivax re-emerged in 1993 and has now become a major public health problem during the summer season in south korea. the aim of this study was to interpret and understand the meaning of seroepidemiological studies for developing the best malaria control programme in south korea.201222852558
species composition, larval habitats, seasonal occurrence and distribution of potential malaria vectors and associated species of anopheles (diptera: culicidae) from the republic of korea.larval mosquito habitats of potential malaria vectors and related species of anopheles from three provinces (gyeonggi, gyeongsangbuk, chungcheongbuk provinces) of the republic of korea were surveyed in 2007. this study aimed to determine the species composition, seasonal occurrence and distributions of anopheles mosquitoes. satellite derived normalized difference vegetation index data (ndvi) was also used to study the seasonal abundance patterns of anopheles mosquitoes.201020163728
the polymorphism and the geographical distribution of the knockdown resistance (kdr) of anopheles sinensis in the republic of korea.in the republic of korea (rok), six sibling species of the anopheles sinensis complex are considered the vector species of malaria, but data on their susceptibilities to malaria and vector capacities have been controversial. the intensive use of insecticides has contributed to the rapid development and spread of insecticide resistance in the an. sinensis complex. knockdown resistance (kdr) to pyrethroids and ddt in the an. sinensis complex is associated with a mutation in codon 1014 of the volta ...201222554130
mosquito species composition and plasmodium vivax infection rates for korean army bases near the demilitarized zone in the republic of korea, 2011.vivax malaria is a significant military and civilian health threat in northern republic of korea (rok). mosquito collections were performed at two rok army installations, paju near the demilitarized zone (dmz) using black light traps in 2011. the dmz, a 4 km wide border, is the northernmost point of the rok and separates the rok from the democratic people's republic of korea (dprk). anopheles spp. were identified by polymerase chain reaction and screened for plasmodium vivax sporozoites. of 4,35 ...201223243112
susceptibility and resistance of field populations of anopheles sinensis (diptera: culicidae) collected from paju to 13 insecticides.over 20% of all malaria cases reported annually in the republic of korea (rok) occur in paju, gyeonggi province. vector control for malaria management is essential, but the insecticide resistance of the vector, anopheles mosquitoes, has been a major obstacle in implementing effective control. in this study, the insecticide resistance of the vector mosquitoes was evaluated and compared with that of vector mosquitoes collected from the same locality in 2001 and 2009.024159535
decreasing incidence of plasmodium vivax in the republic of korea during 2010-2012.after the re-emergence of plasmodium vivax in 1993, a total of 31,254 cases of vivax malaria were reported between 1993-2012 in the republic of korea (rok). the purpose of this study was to review korea centers for disease control and prevention records to investigate the transmission of malaria from 2010-2012.201324010412
historical survey of the kdr mutations in the populations of anopheles sinensis in china in 1996-2014.anopheles sinensis has become an important malaria vector in china. the long-term extensive utilization of pyrethroids for itns and irs for mosquito control in the last three decades has resulted in the occurrence of resistant an. sinensis populations in many regions. knockdown resistance (kdr), caused by point mutations in the vgsc gene, is one of the mechanisms that confer resistance to ddt and pyrethroids. recently, several investigations revealed the kdr occurrence in some an. sinensis popul ...201525888824
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