| further characterization of tomato-infecting begomoviruses in brazil. | tomato cultivation in brazil is threatened by a number of tomato-infecting viruses belonging to the genus begomovirus of the family geminiviridae. here, we report the full dna-a sequences of three brazilian begomoviruses: a potentially new tomato-infecting viruses, tomato interveinal chlorosis virus (toicv), and two previously proposed begomoviruses for which only partial dna-a sequences are available in the databases: tomato mottle leaf curl virus (tmolcv) and tomato golden vein virus (tgvv). t ... | 2012 | 22218964 |
| diversity and prevalence of brazilian bipartite begomovirus species associated to tomatoes. | information on the distribution and prevalence of the economically destructive begomovirus species and recombinant forms infecting fresh-market and processing tomato crops in brazil is crucial in guiding breeding programs and also to understand the evolutionary mechanisms associated with the upsurge of so many species and quasi-species comprising this unique disease complex. an extensive survey was carried out over 3 years (between 2002 and 2004) aiming to study the diversity of begomoviruses in ... | 2008 | 18175211 |
| one-step cloning approach for construction of agroinfectious begomovirus clones. | most begomoviruses have a bipartite genome containing two circular ssdna segments (dna-a and dna-b). routine infectious clone construction relies upon cloning of the whole genome, which is then subcloned as a tandem one-and-half or two genome- (containing two replication origins) cassette into a vector prior to agro-inoculation. the construction of cassettes containing two replication origins is, however, a time-consuming process. here an improved method for rapid construction of agroinfectious ... | 2008 | 18022703 |