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electrophoretic karyotype of intracellular yeast-like symbiotes in rice planthoppers and anobiid beetles.chromosomal dna molecules of intracellular yeast-like symbiotes (yls) of three species of rice planthoppers and two species of anobiid beetles have been separated by pulsed-field gel electrophoresis. probable chromosome numbers of nilaparvata lugens, sogatella furcifera, and laodelphax striatellus, were 4, 4, and 5, respectively, and tentative genome sizes were 17.3, 17.6, and 20.1 mbp, respectively, based upon migration of individual chromosome-sized dna relative to the size standards of schizo ...19957722339
phylogenetic position of yeastlike endosymbionts of anobiid beetles.the anobiid beetles stegobium paniceum and lasioderma serricorne possess the intracellular yeastlike symbionts symbiotaphrina buchneri and symbiotaphrina kochii, respectively, in the mycetome between the foregut and midgut. the nucleotide sequences of the small-subunit rrna-encoding genes of the symbionts were determined for phylogenetic analysis. five group i introns were found in the small-subunit rrna-encoding genes of s. buchneri, but s. kochii lacked introns. the phylogenetic position of bo ...19968572692
assessment of the efficacy of japanese bacillus thuringiensis isolates against the cigarette beetle, lasioderma serricorne (coleoptera: anobiidae).a total of 2,652 japanese isolates of bacillus thuringiensis, belonging to at least 54 h serogroups, were examined for assessment of the toxicity against the cigarette beetle, lasioderma serricorne (coleoptera: anobiidae). when tested with spore/parasporal inclusion mixtures, strong larvicidal activities were associated with 28 isolates (1.1%). serologically, these toxic isolates fell into 4 known h serovars: thuringiensis (9 isolates), kurstaki (2), kenyae (2), and darmstadiensis (15). purified ...200212445796
yeast-like symbiotes as a sterol source in anobiid beetles (coleoptera, anobiidae): possible metabolic pathways from fungal sterols to 7-dehydrocholesterol.insects are unable to synthesize sterols and require exogenous sterol sources for their normal development and reproduction. a few exceptions are insects associated with symbiotic yeasts or fungi. we analyzed sterols by gc-ms in two anobiid beetles (lasioderma serricorne and stegobium paniceum), their intracellular yeast-like symbiotes (yls), and their diets in order to clarify the sterols synthesized by yls and the metabolic pathways of the sterols in the beetles. several c(27), c2(8), and c(29 ...200312655605
enhanced resistance to helicoverpa zea in tobacco expressing an activated form of maize ribosome- inactivating protein.progeny of two transgenic tobacco (nicotiana tabacum l.) lines that expressed an activated form of maize (zea mays l.) ribosome-inactivating protein (rip) had varying resistance to the insect species tested. a subset of r(2) plants from the two lines appeared to be more resistant to larvae of the cigarette beetle, lasioderma serricorne (f.), and the tobacco hornworm, manduca sexta (l.) than the wild type plants. progeny (r(3)) of the more resistant r(2) plants were tested more extensively for in ...200312769526
characterization of a cell-free bacterial extract larvicidal to lasioderma serricorne (cigarette beetle). 196514347939
activity of spinosad on stored-tobacco insects and persistence on cured tobacco stripst.every year raw tobacco and manufactured tobacco products are lost to two major storage pests, the cigarette beetle, lasioderma serricorne (f) and the tobacco moth, ephestia elutella (hiibner). post-harvest management of both insects is achieved through sanitation, insect monitoring and fumigation with phosphine. however, insect resistance to phosphine and control failures have been reported, and fumigants are under constant regulatory pressure. here we report the evaluation of spinosad, a bioins ...200415532683
the effects of the presence of stored product pests on the microfauna of a flour community.to determine the effect that the presence of some beetles have on the species of bacteria found in their flour.200515659205
isolation of bacillus thuringiensis from stored tobacco and lasioderma serricorne (f.).bacillus thuringiensis was isolated from dried tobacco residues and dead tobacco beetles (lasioderma serricorne (f.); coleoptera: anobiidae) collected in a large number of locations worldwide. eighty-eight samples of stored tobacco were analyzed and yielded 78 b. thuringiensis strains which were characterized on the basis of parasporal crystal morphology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles, and the results of an immunoblot analysis of the insecticidal crystal prot ...199416349149
structure of worldwide populations of lasioderma serricorne (coleoptera: anobiidae) as revealed by amplified fragment length polymorphism profiles.the cigarette beetle lasioderma serricorne (fabricius) is the most widespread and destructive pest of stored tobacco. the capability to differentiate between populations from different geographic origins would enable researchers to better understand how insect dispersal through transportation affects the infestation of stored tobacco. using amplified fragment length polymorphism (aflp), dna polymorphisms were assessed in 16 populations of l. serricorne collected from 15 countries. the dendrogram ...200616556331
relative activity of a tobacco hybrid expressing high levels of a tobacco anionic peroxidase and maize ribosome-inactivating protein against helicoverpa zea and lasioderma serricorne.tobacco (nicotiana tabacum) plants grown from seed obtained by crossing a tobacco line that expressed an activated maize ribosome-inactivating protein (rip) with a line that overexpressed tobacco anionic peroxidase were tested for their effects on corn earworm helicoverpa zea and cigarette beetle lasioderma serricorne larvae as compared to the wild-type plant cross. significant feeding reductions were noted for transgenic plants expressing both resistance proteins as compared to wild-type plants ...200616569054
oral toxicity of beta-n-acetyl hexosaminidase to insects.insect chitin is a potential target for resistance plant proteins, but plant-derived chitin-degrading enzymes active against insects are virtually unknown. commercial beta-n-acetylhexosaminidase (naha), a chitin-degrading enzyme from jack bean canavalia ensiformis, caused significant mortality of fall armyworm spodoptera frugiperda larvae at 75 microg/gm, but no significant mortality was noted with aspergillus niger naha. maize zea mays callus transformed to express an arabidopsis thaliana clone ...200717417870
[occurrence of weevils (insecta: coleoptera) in pet food traded in the metropolitan region of recife, pernambuco state, brazil].with the purpose of verifying the occurrence of insect pests in dog food commercialized in the metropolitan region of recife, samples from 15 different pet stores were submitted to the extraction of insects in a berlese-tullgren apparatus. tribolium castaneum (herbst) (tenebrionidae) (55.2%) was the most frequent specie followed by oryzaephilus surinamensis (l.) (cucujidae) (31.3%), rhyzopertha dominica (fabricius) (bostrichidae) (8.9%) and lasioderma serricorne (fabricius) (anobiidae) (4.7%), a ...200819061049
repellant and insecticidal activities of shyobunone and isoshyobunone derived from the essential oil of acorus calamus rhizomes.it was found that the essential oil of acorus calamus rhizomes showed insecticidal activity.026600710
expression of a wolf spider toxin in tobacco inhibits the growth of microbes and insects.lycotoxin i, from the wolf spider (lycosa carolinensis), is an amphipathic pore-forming peptide that has antimicrobial and anti-insect activity. constitutive expression of a lycotoxin i modified for oral toxicity to insects in tobacco (nicotiana tabacum) conferred significantly enhanced resistance to larvae of the corn earworm (helicoverpa zea) and cigarette beetle (lasioderma serricorne). gene expression levels of modified lycotoxin i were negatively correlated to the survival of corn earworm l ...201424770871
[furnishing of sterol by the yeast-like endosymbionts of lasioderma serricorne f. (coleoptera, anobiidae) and its ecological importance for the host].by comparing the growth of normal and aposymbiontic lasioderma serricorne on diets of increasing cholesterol amounts, we demonstrated that the endosymbionts furnish sterols in quantities comparable to a cholesterol concentration of 0.01-0.03% in the diet (fig. 1). we reared aposymbiontic larvae in diets without added sterol, which contained 50% of samples of different woods or cigarette tobacco. with fresh aesculus wood and tobacco, larval growth was optimal. other fresh woods (acer, prunus, fag ...197428308799
[the vitamin requirements of normal and aposymbiotic lasioderma serricorne f. (coleoptera, anobiidae) and the role of symbiotic fungi as vitamin sources for their hosts].normal lasioderma serricorne were reared on a totally synthetic, vitamin-free diet for 4 subsequent generations, though development was substantially retarded when compared with that in a diet containing all b-vitamins and a sterol necessary for growth of aposymbiotic larvae. comparing growth in the presence or absence of the symbionts on diets with a single vitamin omitted it was demonstrated that the microorganisms are able to supply thiamin, lactoflavin, pyridoxine, nicotinic acid, pantotheni ...196928309206
bioactivity of essential oil of artemisia argyi lévl. et van. and its main compounds against lasioderma serricorne.artemisia argyi lévl. et van., a perennial herb with a strong volatile odor, is widely distrbuted in the world. essential oil obtained from artemisia argyi was analyzed by gas chromatography-mass spectrometry (gc-ms). a total of 32 components representing 91.74% of the total oil were identified and the main compounds in the oil were found to be eucalyptol (22.03%), β-pinene (14.53%), β-caryophyllene (9.24%) and (-)-camphor (5.45%). with a further isolation, four active constituents were obtained ...201425017866
the chemical composition of essential oils from cinnamomum camphora and their insecticidal activity against the stored product pests.to investigate the chemical composition and insecticidal activity of the essential oils of certain chinese medicinal herbs and spices, the essential oils were extracted from the stem barks, leaves, and fruits of cinnamomum camphora (l.) presl, which were found to possess strong fumigant toxicity against tribolium castaneum and lasioderma serricorne adults. the essential oils of the plants were extracted by the method of steam distillation using a clavenger apparatus. their composition was determ ...201627827929
contact and fumigant activities of constituents of foeniculum vulgare fruit against three coleopteran stored-product insects.the insecticidal activities of materials derived from the fruit of fennel, foeniculum vulgare, against adults of sitophilus oryzae, callosobruchus chinensis and lasioderma serricorne were examined using direct contact application and fumigation methods. the biologically active constituents of the foeniculum fruits were characterized as the phenylpropenes (e)-anethole and estragole, and the monoterpene (+)-fenchone, by spectroscopic analysis. responses varied with insect species, compound, dose a ...200111455661
insect pests and integrated pest management in museums, libraries and historic buildings.insect pests are responsible for substantial damage to museum objects, historic books and in buildings like palaces or historic houses. different wood boring beetles (anobium punctatum, hylotrupes bajulus, lyctus sp. or introduced species), the biscuit beetle (stegobium paniceum), the cigarette beetle (lasioderma serricorne), different dermestides (attagenus sp., anthrenus sp., dermestes sp., trogoderma sp.), moths like the webbing clothes moth (tineola bisselliella), silverfish (lepisma sacchar ...201526463205
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