| bacillus aerius sp. nov., bacillus aerophilus sp. nov., bacillus stratosphericus sp. nov. and bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. | four novel bacterial strains were isolated from cryogenic tubes used to collect air samples at altitudes of 24, 28 and 41 km. the four strains, 24k(t), 28k(t), 41kf2a(t) and 41kf2b(t), were identified as members of the genus bacillus. phylogenetic analysis based on 16s rrna gene sequences indicated that three of the strains, 24k(t), 28k(t) and 41kf2a(t), are very similar to one another (>98 % sequence similarity) and show a similarity of 98-99 % with bacillus licheniformis and 98 % with bacillus ... | 2006 | 16825614 |
| carotenoids found in bacillus. | to identify the diversity of pigmented aerobic spore formers found in the environment and to characterize the chemical nature of this pigmentation. | 2010 | 19878522 |
| a novel serine alkaline protease from bacillus altitudinis gvc11 and its application as a dehairing agent. | a serine alkaline protease from a newly isolated alkaliphilic bacillus altitudinis gvc11 was purified and characterized. the enzyme was purified to homogeneity by acetone precipitation, deae-cellulose anion exchange chromatography with 7.03-fold increase in specific activity and 15.25% recovery. the molecular weight of alkaline protease was estimated to be 28 kda by sds page and activity was further assessed by zymogram analysis. the enzyme was highly active over a wide range of ph 8.5 to 12.5 w ... | 2010 | 21080200 |
| enhancing jatropha oil extraction yield from the kernels assisted by a xylan-degrading bacterium to preserve protein structure. | we investigated the use of bacterial cells isolated from paddy crab for the extraction of oil from jatropha seed kernels in aqueous media while simultaneously preserving the protein structures of this protein-rich endosperm. a bacterial strain-which was marked as mb4 and identified by means of 16s rdna sequencing and physiological characterization as either bacillus pumilus or bacillus altitudinis-enhanced the extraction yield of jatropha oil. the incubation of an mb4 starter culture with prehea ... | 2011 | 21556918 |
| genetic diversity and phylogeny of antagonistic bacteria against phytophthora nicotianae isolated from tobacco rhizosphere. | the genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by boxair-pcr, 16s-rflp, 16s rrna sequence homology and phylogenetic analysis methods. these studies revealed that 4.01% of the 6652 tested had some inhibitory activity against phytophthora nicotianae. boxair-pcr analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. a total of 25 16s-rflp patterns were iden ... | 2011 | 21686169 |
| hydrogen peroxide-resistant cota and yjqc of bacillus altitudinis spores are a promising biocatalyst for catalyzing reduction of sinapic acid and sinapine in rapeseed meal. | for the more efficient detoxification of phenolic compounds, a promising avenue would be to develop a multi-enzyme biocatalyst comprising peroxidase, laccase and other oxidases. however, the development of this multi-enzyme biocatalyst is limited by the vulnerability of fungal laccases and peroxidases to hydrogen peroxide (h2o2)-induced inactivation. therefore, h2o2-resistant peroxidase and laccase should be exploited. in this study, h2o2-stable cota and yjqc were isolated from the outer coat of ... | 2016 | 27362423 |
| antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia). | antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ... | 2015 | 25803299 |
| identification of strains bacillus aerophilus mtcc 7304t as bacillus altitudinis and bacillus stratosphericus mtcc 7305t as a proteus sp. and the status of the species bacillus aeriusshivaji et al. 2006. request for an opinion. | on the basis of 16s rrna, rpob, gyrb and pyca gene sequence analyses, characterization of biochemical features and other phenotypic traits and pulsed-field gel electrophoresis (pfge) fingerprinting, it was ascertained that strains bacillus aerius mtcc 7303t, bacillus aerophilus mtcc 7304(t) and bacillus stratosphericus mtcc 7305(t) do not conform to the descriptions of the type strains of the respective species. strains mtcc 7303(t) and mtcc 7304(t) were indistinguishable from bacillus altitudin ... | 2015 | 26297145 |
| bacillus xiamenensis sp. nov., isolated from intestinal tract contents of a flathead mullet (mugil cephalus). | a taxonomic study was carried out on strain hyc-10(t), which was isolated from the intestinal tract contents of a flathead mullet, mugil cephalus, captured from the sea off xiamen island, china. the bacterium was observed to be gram positive, oxidase and catalase positive, rod shaped, and motile by subpolar flagella. the bacterium was found to grow at salinities of 0-12 % and at temperatures of 8-45 °c. the isolate was found to hydrolyze aesculin and gelatin, but was unable to reduce nitrate to ... | 2014 | 24158533 |
| isolation and characterization of bacterial isolates algicidal against a harmful bloom-forming cyanobacterium microcystis aeruginosa. | algicidal bacteria mai11-2, mai11-5 and mai11-10, which inhibited the growth of a harmful bloom-forming cyanobacterium microcystis aeruginosa, were isolated from a sewage treatment plant. the isolate mai11-5 was phylogenetically affiliated into the genus pedobacter, while mai11-2 and mai11-10 were closely related to bacillus aerophilus, bacillus altitudinis and bacillus stratosphericus with 100% identity based on 16s ribosomal rna sequences. co-cultivation of m. aeruginosa with the algicidal iso ... | 2012 | 23007101 |
| dynamics of bacterial class bacilli in the deepest valley lake of kashmir-the manasbal lake. | in recognition of the importance of bacteria as ecological indicators of the aquatic systems a comprehensive and systematic analysis was carried out on manasbal lake, the deepest spring fed valley lake of kashmir. the main objective envisaged was to analyze bacterial community composition (bcc) and for this purpose systematic and regular sampling of waters from ten different sampling stations, predetermined in the lake according to differences in degree of human interference and also as zones of ... | 2017 | 28087491 |
| plant growth-promotion and biofortification of chickpea and pigeonpea through inoculation of biocontrol potential bacteria, isolated from organic soils. | seven strains of bacteria [pseudomonas plecoglossicida sri-156, brevibacterium antiquum sri-158, bacillus altitudinis sri-178, enterobacter ludwigii sri-211, e. ludwigii sri-229, acinetobacter tandoii sri-305 and pseudomonas monteilii sri-360; demonstrated previously for control of charcoal rot disease in sorghum and plant growth-promotion (pgp) in rice] were evaluated for their pgp and biofortification traits in chickpea and pigeonpea under field conditions. when treated on seed, the seven sele ... | 2016 | 27833841 |
| enhanced cd extraction of oilseed rape (brassica napus) by plant growth-promoting bacteria isolated from cd hyperaccumulator sedum alfredii hance. | four plant growth-promoting bacteria (pgpb) were used as study materials, among them two heavy metal-tolerant rhizosphere strains srn1 (arthrobacter sp.) and srn9 (bacillus altitudinis) were isolated from rhizosphere soil, while two endophytic strains san1 (bacillus megaterium) and samr12 (sphingomonas) were identified from roots of the cadmium (cd)/zinc (zn) hyperaccumulator sedum alfredii hance. a pot experiment was carried out to investigate the effects of these pgpb on plant growth and cd ac ... | 2017 | 27593491 |
| simultaneous preconcentrations of co(2+), cr(6+), hg(2+) and pb(2+) ions by bacillus altitudinis immobilized nanodiamond prior to their determinations in food samples by icp-oes. | a novel solid phase extraction method was developed for simultaneous preconcentration-separation of co(2+), cr(6+), hg(2+) and pb(2+) ions prior to their determinations in food samples by icp-oes. thermophilic bacillus altitudinis immobilized nanodiamond was used as a new biosorbent. sem and ft-ir analysis were studied to characterize the biosorbent. the optimum ph values of quantitative biosorption for co(2+), cr(6+), hg(2+) and pb(2+) were found to be 5.0, 6.0, 6.0 and 6.0, respectively. a flo ... | 2017 | 27542497 |
| identification of "bacillus cellulasensis" strain nio-1130(t) as a member of bacillus altitudinis and emendation of the latter. | in the study by mawlankar et al. in arch microbiol 198:83-89 (2016), the phylogenetic position of strain "bacillus cellulasensis" nio-1130(t) based on 16s rrna and gyrb genes was inconsistent. therefore, the aim of this study is to re-determine its taxonomic status using diverse genotypic approaches including single gene analysis, multilocus sequence analysis, and genomic analyses. the reconstructed phylogenetic trees based on 16s rrna gene and six concatenated genes showed that "b. cellulasensi ... | 2016 | 27339257 |
| three-step procedure for preparation of pure bacillus altitudinis ribonuclease. | ribonucleases are considered as promising tools for anticancer treatment due to their selective cytotoxicity against tumor cells. we investigated a new rnase from bacillus altitudinis termed balnase (b. altitudinis rnase). balnase is a close homolog of the well-known cytotoxic binase, differing by only one amino acid residue: nonpolar hydrophobic alanine at position 106 in the balnase molecule is replaced by a polar uncharged threonine in binase. the most exciting question is how the physico-che ... | 2016 | 27047739 |
| differential toxicity of al2o3 particles on gram-positive and gram-negative sediment bacterial isolates from freshwater. | the current study was aimed to explore the differential effects on gram-positive and gram-negative freshwater sediment bacterial isolates upon exposure to nano-particles and bulk particles of al2o3 at low concentrations (0.25, 0.5, and 1 mg/l). the gram-negative pseudomonas aeruginosa was more susceptible to both the nano-forms and bulk forms than the gram-positive bacillus altitudinis. the generation of reactive oxygen species (ros) and release of lipopolysaccharide due to membrane damage were ... | 2016 | 26965278 |
| bacillus zhangzhouensis sp. nov. and bacillus australimaris sp. nov. | two strains, designated dw5-4t and nh7i_1t, were isolated from aquaculture water of shrimp farm of zhangzhou city in china and surface sediment of the south china sea, respectively. cells of the strains were gram-stain-positive, oxidase- and catalase-positive, rod-shaped and motile. the phylogenetic analyses based on 16s rrna gene sequences indicated that two strains belonged to the bacillus pumilus clade. the similarities of 16s rrna gene sequences between two strains dw5-4t and nh7i_1t was 99. ... | 2015 | 26705002 |
| identification of bacillus strains by maldi tof ms using geometric approach. | microorganism identification by maldi tof mass-spectrometry is based on the comparison of the mass spectrum of the studied organism with those of reference strains. it is a rapid and reliable method. however, commercial databases and programs are mostly designed for identification of clinically important strains and can be used only for particular mass spectrometer models. the need for open platforms and reference databases is obvious. in this study we describe a geometric approach for microorga ... | 2015 | 26592761 |
| the response of maize (zea mays l.) plant assisted with bacterial consortium and fertilizer under oily sludge. | the objective of this study was to evaluate the role of pgpr consortium and fertilizer alone and in combination on the physiology of maize grown under oily sludge stress environment as well on the soil nutrient status. consortium was prepared from bacillus cereus (acc kr232400), bacillus altitudinis (acc kf859970), comamonas (delftia) belonging to family comamonadacea (acc kf859971) and stenotrophomonasmaltophilia (acc kf859973). the experiment was conducted in pots with complete randomized desi ... | 2016 | 26587972 |
| reclassification of bacillus invictae as a later heterotypic synonym of bacillus altitudinis. | the aim of this study was to reclarify the taxonomic status of strain bacillus invictae bi.ffup1t by performing comparative analyses with the other four type strains within the bacillus pumilus group. the digital dna-dna hybridization (dddh) and average nucleotide identity (ani) values between strains b. invictae bi.ffup1t ( = dsmz 26896t = mccc 1a07089t), b. altitudinis 41kf2bt ( = dsmz 21631t = mccc 1a06452t), b. safensis fo-36bt ( = dsmz 19292t = mccc 1a6451t), b. pumilus atcc 7061t ( = dsmz ... | 2015 | 25977285 |
| xylanases of bacillus spp. isolated from ruminant dung as potential accessory enzymes for agro-waste saccharification. | polysaccharide hydrolase producing bacteria were isolated for biomass saccharification step in two-step bioethanol production. xylanolytic bacteria were found to be common in ruminant dung. seven bacillus dung isolates exhibited high xylanolytic activity; three of which were identified as bacillus safensis and four as bacillus altitudinis, based on 16s rdna and gyrb gene sequencing. interestingly, comparison of activity profiles for b. safensis m35 and b. altitudinis r31 and j208 crude xylanases ... | 2015 | 25645626 |
| draft whole-genome sequence of bacillus altitudinis strain b-388, a producer of extracellular rnase. | here, we present a draft genome sequence of bacillus altitudinis strain b-388, including a putative plasmid. the strain was isolated from the intestine of indian meal moth, a common pest of stored grains, and it is characterized by the production of extracellular rnase, similar to binase, which is of interest for comparative studies and biotechnology. | 2015 | 25635022 |
| engineering of a thermostable β-1,3-1,4-glucanase from bacillus altitudinis yc-9 to improve its catalytic efficiency. | error-prone polymerase chain reaction (pcr) is frequently used in directed evolution of enzymes to modify their quality. in this study, error-prone pcr was used to improve the catalytic efficiency of β-1,3-1,4-glucanase from bacillus altitudinis yc-9. | 2016 | 25546703 |
| bacillus invictae sp. nov., isolated from a health product. | a gram-positive, rod-shaped, endospore-forming bacillus isolate, bi.(ffup1) (t), recovered in portugal from a health product was subjected to a polyphasic study and compared with the type strains of bacillus pumilus, bacillus safensis, bacillus altitudinis and bacillus xiamenensis, the phenotypically and genotypically most closely related species. acid production from cellobiose, d-glucose and d-mannose and absence of acid production from d-arabinose, erythritol, inositol, maltose, mannitol, raf ... | 2014 | 25171924 |
| [weathering of potash feldspar by bacillus sp. l11]. | to determine the taxonomic position of mineral-weathering bacterium l11 isolated from soil of potassium mine tailing of nanjing and to elucidate the weathering mechanism of the strain, which will offer the basis for the interaction between microorganism and mineral. | 2013 | 24617258 |
| biocontrol efficacy and plant growth promoting activity of bacillus altitudinis isolated from darjeeling hills, india. | a total of 18 bacterial isolates were obtained from the rhizosphere of sechium edule growing in the lower foothills of darjeeling, india. the bacterial isolates were tested for pgpr traits in vitro such as phosphate solubilization, hcn, siderophore, iaa, chitinase, protease production as well as inhibition of pthytopathogens. of all the bacterial isolates, one bacterium designated as brhs/s-73 was found to possess all the tested characters which was identified on the basis of 16s rrna gene seque ... | 2015 | 23996212 |
| biodiversity of aerobic endospore-forming bacterial species occurring in yanyanku and ikpiru, fermented seeds of hibiscus sabdariffa used to produce food condiments in benin. | yanyanku and ikpiru made by the fermentation of malcavene bean (hibiscus sabdariffa) are used as functional additives for parkia biglobosa seed fermentations in benin. a total of 355 aerobic endospore-forming bacteria (aefb) isolated from yanyanku and ikpiru produced in northern and southern benin were identified using phenotypic and genotypic methods, including gtg5-pcr, m13-pcr, 16s rrna, gyra and gyrb gene sequencing. generally, the same 5-6 species of the genus bacillus predominated: bacillu ... | 2013 | 23571124 |
| purification, characterization, and heterologous expression of a thermostable β-1,3-1,4-glucanase from bacillus altitudinis yc-9. | purification, characterization, gene cloning, and heterologous expression in escherichia coli of a thermostable β-1,3-1,4-glucanase from bacillus altitudinis yc-9 have been investigated in this paper. the donor strain b. altitudinis yc-9 was isolated from spring silt. the native enzyme was purified by ammonium sulfate precipitation, diethylaminoethyl-cellulose anion exchange chromatography, and sephadex g-100 gel filtration. the purified β-1,3-1,4-glucanase was observed to be stable at 60 °c and ... | 2013 | 23292246 |
| production of alkaline protease by bacillus altitudinis gvc11 using castor husk in solid-state fermentation. | castor (ricinus communis l.) is an important oil seed crop having its main cultivated area in india, china, and brazil in dry land farming. castor husk is generated as waste in castor oil production. use of castor husk waste as substrate is studied for alkaline protease production by bacillus altitudinis gvc11 in solid-state fermentation. various parameters like moisture content, incubation period, particle size, effect of carbon and nitrogen sources are studied and optimized for enzyme producti ... | 2012 | 22415781 |
| evaluation of bacteria isolated from rice rhizosphere for biological control of charcoal rot of sorghum caused by macrophomina phaseolina (tassi) goid. | a total of 360 bacteria, isolated from the rhizospheres of a system of rice intensification (sri) fields, were characterized for the production of siderophore, fluorescence, indole acetic acid (iaa), hydrocyanic acid (hcn) and solubilization of phosphorus. of them, seven most promising isolates (sri-156, -158, -178, -211, -229, -305 and -360) were screened for their antagonistic potential against macrophomina phaseolina (causes charcoal rot in sorghum) by dual culture assay, blotter paper assay ... | 2011 | 25187130 |
| phylogenetic and clonality analysis of bacillus pumilus isolates uncovered a highly heterogeneous population of different closely related species and clones. | bacillus pumilus is a gram-positive bacterium with a wide range of attributed applications, namely as a plant growth promoting rhizobacteria (pgpr), animal, and human probiotic. however, a rare putative role in human diseases has been reported, namely in food poisoning or as anthrax-like cutaneous infectious agent. this species is difficult to distinguish from its closely related species on the basis of phenotypic or biochemical characteristics and 16s rrna gene sequences. in this study, the phy ... | 2014 | 25230950 |
| biological control of heterodera glycines by spore-forming plant growth-promoting rhizobacteria (pgpr) on soybean. | heterodera glycines, the soybean cyst nematode, is the most economically important plant-parasitic nematode on soybean production in the u.s. the objectives of this study were to evaluate the potential of plant growth-promoting rhizobacteria (pgpr) strains for mortality of h. glycines j2 in vitro and for reducing nematode population density on soybean in greenhouse, microplot, and field trials. the major group causing mortality to h. glycines in vitro was the genus bacillus that consisted of 92. ... | 2017 | 28704498 |
| draft genome sequence of bacillus altitudinis ynp4-tsu, isolated from yellowstone national park. | undisturbed hot springs inside yellowstone national park remain a dynamic biome for novel cellulolytic thermophiles. we report here the draft genome sequence of one of these isolates, bacillus altitudinis ynp4-tsu. | 2017 | 28705979 |
| chicken egg shell as a potential substrate for production of alkaline protease by bacillus altitudinis gvc11 and its applications. | chicken egg shell and membrane were used as substrate for production of alkaline protease by bacillus altitudinis gvc11. maltose as additional carbon source enhanced enzyme production up to 13%. addition of organic nitrogen sources like peptone and yeast extract increased enzyme production by 9% and 5%, respectively and inorganic nitrogen sources did not have any positive effect. the resultant protein hydrolyzate after fermentation was found to have essential amino acids such as leucine, phenyl ... | 2017 | 28664373 |
| draft genome report of bacillus altitudinis sorb11, isolated from the indian sector of the southern ocean. | here, we present the draft genome sequence of bacillus altitudinis sorb11, which is tolerant to uv radiation. the strain was isolated from the indian sector of the southern ocean at a depth of 3.8 km. the genome sequence information reported here for b. altitudinis sorb11 gives the basis of its uv resistance mechanism and provides data for further comparative studies with other bacteria resistant to uv radiation. | 2017 | 28596391 |
| endophytic bacteria isolated from panax ginseng improves ginsenoside accumulation in adventitious ginseng root culture. | ginsenoside is the most important secondary metabolite of ginseng. natural sources of wild ginseng have been overexploited. although root culture could reduce the length of the growth cycle of ginseng, the number of ginsenosides is fewer and their contents are lower in adventitious roots of ginseng than that in ginseng cultivated in the field. in this study, we investigated the effects of endophytic bacterial elicitors on biomass and ginsenoside production in adventitious roots cultures of panax ... | 2017 | 28545250 |
| surfactant- and oxidant-stable alkaline proteases from bacillus invictae: characterization and potential applications in chitin extraction and as a detergent additive. | a newly alkaline proteases producing strain was isolated from sea water. the strain was identified as bacillus invictae on the basis of biochemical characteristics and 16s rrna sequence analysis. the crude protease activity showed an optimal activity at approximately 60°c and in wide ph interval ranging from 9.0 to 11.0. at least six clear caseinolytic protease bands were observed in a zymogram. phenylmethylsulfonyl fluoride (pmsf), a serine-protease inhibitor, was found to inhibit completely th ... | 2017 | 27988295 |