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novel cellulase screening and optimal production from the wood decaying xylariaceae: daldinia species.the highest cellulases production from daldinia caldariorum 263 (d-263) was found among daldinia eschscholzii and daldinia childiae. three cellulases, one xylanase and one β-glucosidase of the molecular weights 55, 43, 34, 30, and 105 kda, respectively, were determined by zymographic sodium dodecyl sulfate polyacrylamide gel electrophoresis. from the n-terminal sequencing, the major cellulase cela belonging to glycosyl hydrolase family 5 was determined. by following an orthogonal experiment desi ...201020960239
unprecedented immunosuppressive polyketides from daldinia eschscholzii, a mantis-associated fungus. 200818576440
immunosuppressive polyketides from mantis-associated daldinia eschscholzii.polyketides with unknown architectures are highly desired for the discovery of new drugs and agrochemicals. here, the mantis-associated daldinia eschscholzii, a fungus known to produce immunosuppressants dalesconols a and b, was found to simultaneously generate four novel skeletons capable of shaping the unusual chemistry of the fungal polyketides, of which seven were structurally unique and substantially immunosuppressive. in particular, the scaled-up fermentation of the microbe enabled the str ...201121434637
purification and biochemical characterization of an extracellular beta-glucosidase from the wood-decaying fungus daldinia eschscholzii (ehrenb.:fr.) rehm.an extracellular beta-glucosidase was purified from culture filtrates of the wood-decaying fungus daldinia eschscholzii (ehrenb.:fr.) rehm grown on 1.0% (w/v) carboxymethyl-cellulose using ammonium sulfate precipitation, ion-exchange, hydrophobic interaction and gel filtration chromatography. the enzyme is monomeric with a molecular weight of 64.2 kda as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and has a pi of 8.55. the enzyme catalyzes the hydrolysis of p-nitrophe ...200717439636
concentricol, a taxonomically significant triterpenoid from daldinia concentrica.in the course of a chemotaxonomic study of xylariaceous ascomycetes, a major metabolite was isolated and identified from the ascostromata of daldinia concentrica. the compound, for which the name concentricol is proposed, constitutes a highly oxidised squalene derivative. a survey of several daldinia spp. from around the world (including several type materials), employing analytical hplc-uv/vis (with diode array detection) and positive electrospray hplc-ms of stromatal meoh extracts revealed tha ...200111324905
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