| proposal of the genus thermoactinomyces sensu stricto and three new genera, laceyella, thermoflavimicrobium and seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analyses. | phylogenetic analysis based on 16s rrna gene sequences revealed that thermoactinomyces species with validly published names can be assigned to four clusters or lineages. the type strains of thermoactinomyces sacchari and thermoactinomyces putidus were differentiated from the type strains of thermoactinomyces vulgaris and thermoactinomyces intermedius by the predominant menaquinone and fatty acid profiles. the type strains of thermoactinomyces dichotomicus and thermoactinomyces peptonophilus form ... | 2005 | 15653907 |
| occurrence and characterization of actinobacteria and thermoactinomycetes isolated from pulp and board samples containing recycled fibres. | the aim of this study was to characterize the actinobacterial population present in pulps and boards containing recycled fibres. a total of 107 isolates was identified on the basis of their pigmentation, morphological properties, fatty acid profiles and growth temperature. of the wet pulp and water sample isolates (n=87), 74.7% belonged to the genus streptomyces, 17.2% to nocardiopsis and 8.0% to thermoactinomycetes, whereas all the board sample isolates (n=20) were thermoactinomycetes. the iden ... | 2006 | 16292555 |
| two novel cyclodextrin-degrading enzymes isolated from thermophilic bacteria have similar domain structures but differ in oligomeric state and activity profile. | in this paper, we present the expression and characterization of two novel enzymes from the alpha-amylase family exhibiting cyclomaltodextrinase specificity. the nucleotide sequences encoding the enzymes were isolated from the genomic dna of two thermophilic bacterial strains originating from icelandic hot springs and belonging to the genera anoxybacillus (afcda13) and laceyella (lscda13). the genes were amplified using a consensus primer strategy utilizing two of the four conserved regions pres ... | 2005 | 16310726 |
| characterisation of two novel cyclodextrinases using on-line microdialysis sampling with high-performance anion exchange chromatography. | in this work, a real-time sampling/analytical method for on-line measurements of two newly discovered cyclomaltodextrinases (cdases) has been developed and evaluated. this novel methodology not only allows the final products to be investigated, but it also reveals enzyme-specific differences in the degradation pathways during the hydrolysis of different substrates, which is a great advantage in the important tasks of investigating the mechanisms of and classifying new hydrolases, and is an advan ... | 2006 | 16804672 |
| desmospora activa gen. nov., sp. nov., a thermoactinomycete isolated from sputum of a patient with suspected pulmonary tuberculosis, and emended description of the family thermoactinomycetaceae matsuo et al. 2006. | a novel gram-positive, aerobic, catalase-positive, filamentous micro-organism, designated strain immib l-1269(t), originating from sputum was characterized using phenotypic and molecular taxonomic methods. it showed cell-wall chemotype iii, phospholipid type pii (with phosphatidylethanolamine as the diagnostic phospholipid) and contained an unsaturated menaquinone with seven isoprene units (mk-7) as the predominant isoprenoid quinone. it synthesized long-chain cellular fatty acids of the straigh ... | 2009 | 19244421 |
| detection and molecular characterization of filamentous actinobacteria and thermoactinomycetes present in water-damaged building materials. | in this study the dominant filamentous actinobacteria occurring in water-damaged building materials were detected by culture and characterized by automated ribotyping and 16s rrna gene sequencing. fifty-two samples were taken from 20 water-damaged houses in four different countries. a total of 122 bacterial isolates were analyzed. actinobacteria or thermoactinomycetes were present in 48% of the samples. the dominant genus was streptomyces (58% of isolates), followed by thermoactinomyces (23%), l ... | 2009 | 19302504 |
| alkalithermophilic actinomycetes in a subtropical area of jujuy, argentina. | the objective of this study was to examine the alkalithermophilic actinomycete communities in the subtropical environment of jujuy, argentina, characterized by sugarcane crops. laceyella putida, laceyella sacchari, thermoactinomyces intermedius, thermoactinomyces vulgaris and thermoflavimicrobium dichotomicum were isolated on the media with novobiocin, from sugar cane plants and renewal rhizospheres, and grass and wood soils. soil ph was almost neutral or lightly alkaline, except for grass soil ... | 2009 | 19623902 |
| laceyella tengchongensis sp. nov., a thermophile isolated from soil of a volcano. | a thermophilic strain, designated yim 10002(t), was isolated from a soil sample of big empty volcano in tengchong county, yunnan province, south-west china, and a polyphasic approach was used to investigate its taxonomic position. strain yim 10002(t) formed endospores on both aerial and substrate mycelia. whole-cell hydrolysates contained meso-diaminopimelic acid, ribose, xylose and glucose. the major fatty acids were iso-c(15 : 0) and anteiso-c(15 : 0). the predominant menaquinone was mk-9. the ... | 2010 | 19915116 |
| laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring in china. | a thermophilic bacterium strain, designated rha1t, was isolated from a sedimental sample of a hot spring collected from tengchong county, yunnan province, south-west china and was characterized by using a polyphasic approach. the strain was affiliated to the genus laceyella based on its phenotypic and phylogenetic characteristics. the strain rha1t formed white aerial and yellow-white substrate mycelia, bearing single endospore on short sporophores. the whole cell hydrolysates contained meso-diam ... | 2011 | 21317276 |