| characterization of virus-like particles assembled in a recombinant baculovirus system expressing the capsid protein of a fish nodavirus. | betanodaviruses are causative agents of neurological disorders in several species of fish. we cloned and sequenced the rna2 segment of two grouper viruses isolated from epinephelus malabaricus (malabaricus grouper nervous necrosis virus, mgnnv) and epinephelus lanceolatus (dragon grouper nervous necrosis virus, dgnnv). the sequences of the two rnas were 99% identical and comparison with previously sequenced rna2 segments of fish nodaviruses striped jack nervous necrosis virus, atlantic halibut v ... | 2001 | 11883005 |
| early endocytosis pathways in ssn-1 cells infected by dragon grouper nervous necrosis virus. | many fish undergo betanodavirus infection. to study the infection process of dragon grouper nervous necrosis virus (dgnnv), native virus and virus-like particles (vlps) were used to analyse the binding and internalization in ssn-1 cells. the binding of dgnnv and vlps to ssn-1 cells was demonstrated using western blotting and immunofluorescence microscopy. as estimated by indirect elisa, the dgnnv particles bound ssn-1 cells in a dose-dependent manner up to 8 x 10(4) particles per cell. the bindi ... | 2005 | 16099914 |
| role of the dxxdxd motif in the assembly and stability of betanodavirus particles. | piscine betanodavirus possesses a bipartite genome of single-stranded (+)rnas. rna2 cdna of dragon grouper nervous necrosis virus (dgnnv) has been expressed previously to form virus-like particles (vlps), which are highly similar to the native virion. experiments with calcium-chelating or reducing/oxidizing reagents showed that the dgnnv vlps required only calcium for particle assembly. with the recombinant vlps, site-directed mutagenesis can be employed to investigate the roles of calcium-bindi ... | 2008 | 18626568 |
| roles of cysteines cys115 and cys201 in the assembly and thermostability of grouper betanodavirus particles. | the virus-like particle (vlp) assembled from capsid subunits of the dragon grouper nervous necrosis virus (dgnnv) is very similar to its native t = 3 virion. in order to investigate the effects of four cysteine residues in the capsid polypeptide on the assembly/dissociation pathways of dgnnv virions, we recombinantly cloned mutant vlps by mutating each cysteine to destroy the specific disulfide linkage as compared with thiol reduction to destroy all s-s bonds. the mutant vlps of c187a and c331a ... | 2010 | 20446029 |