| na+ and k+ concentration and regulation of protein synthesis in l-a9 and aedes albopictus cells infected with marituba virus (bunyaviridae). | infection of l-a9 cells with marituba virus produces a severe inhibition of protein synthesis. this inhibition is temporally correlated with an increase in the intracellular na+ concentration and a decrease in the intracellular k+ concentration. however, in marituba virus-infected aedes albopictus cells the intracellular level of na+ and k+ ions and protein synthesis remained unaltered. incubation of both cell types at high nacl concentration facilitated the translation of viral rna whereas the ... | 1989 | 2607344 |
| the surface charge of l-a9 cells and aedes albopictus cells infected with marituba (bunyaviridae) virus. | the surface charge of marituba virus infected l-a9 cells and aedes albopictus cells was estimated by direct measurement of their electrophoretic mobilities. uninfected l-a9 cells and a. albopictus cells have mean electrophoretic mobilities of -1.083 microns/s x cm/v and -1.019 microns/s x cm/v, respectively. in marituba virus infected l-a9 cells a progressive decline in the electrophoretic mobility was observed. in contrast, in marituba virus infected a. albopictus cells the electrophoretic mobi ... | 1987 | 3111119 |
| selective inhibition of protein synthesis by hypertonic medium in marituba (bunyaviridae) virus-infected l-a9 cells. | elevation of the nacl concentration in the growth medium of l-a9 cells caused an inhibition of the protein synthesis accompanied by a complete breakdown of polyribosomes. however, a complete recovery of the rate of protein synthesis was observed when isotonicity was restored. in marituba virus infected cells, protein synthesis became resistant to hypertonic treatment. under hypertonic conditions cellular protein synthesis was selectively suppressed and an enhancement of virus proteins was observ ... | 1987 | 3680461 |
| marituba (bunyaviridae) virus replication in cultured aedes albopictus cells and in l-a9 cells. | the replication of marituba virus (bunyavirus genus, family bunyaviridae), was studied in aedes albopictus (mosquito) cells. infection of aedes albopictus cells with marituba virus was characterized by an initial acute phase of infection in which large amounts of virus were produced and further by a persistent phase of infection in which virus yield was much lower. no changes in host cell dna, rna and protein synthesis were observed in aedes albopictus cells infected with marituba virus. in cont ... | 1986 | 3729731 |
| interferon induction in mouse fibroblast l-a9 cells. | mouse l-a9 cell interferon was induced by infection with newcastle disease virus. interferon production was 1.5 x 10(5) iu/10(7) cells. interferon was partially purified by precipitation with ammonium sulphate, chromatography on cm-sephadex and hydrophobic chromatography on octyl-agarose. the specific activity of the final preparation was 1.7 x 10(7) iu/mg protein. treatment of l-a9 cells with 20 iu/ml interferon prior to viral infection inhibited the intracellular accumulation of reovirus-speci ... | 1985 | 3835978 |
| macromolecular synthesis in l-a9 cells infected with marituba virus. | | 1981 | 6168249 |
| autointerference of marituba virus (bunyaviridae) in mouse l cells by defective interfering particles. | the growth characteristics of marituba virus, a member of the bunyaviridae family, were studied in l-a9 cells. virus yield was strictly dependent on the moi. quantitation of infectious virus released from the cells revealed a decrease in magnitude with continued serial passage. specificity of the marituba virus inhibitory response was investigated in relation to interference within homologous and heterologous viral classes. virus particles were studied by isopycnic centrifugation in sucrose grad ... | 1983 | 6629698 |
| structural proteins of marituba virus (bunyaviridae). | marituba virus was purified by rate-zonal sedimentation and equilibrium density centrifugation in sucrose gradients. the buoyant density of virus particles was 1.19 g/ml. purified virus was dissociated and its proteins were analyzed by sds-page. four virus polypeptides were identified and designated l, g1, g2 and n. their average molecular weights were 190 x 10(3) [l, range (180-200) x 10(3)], 120 x 10(3) [g1, range (118-122) x 10(3)], 26 x 10(3) [g2, range (24-28) x 10(3)], and 20 x 10(3) [n, r ... | 1983 | 6629700 |