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pseudomonas matsuisoli sp. nov., isolated from a soil sample.an aerobic, gram-stain-negative, rod-shaped and polar-flagellated bacterium, designated strain cc-mhh0089(t), was isolated from a soil sample taken on matsu island (taiwan). strain cc-mhh0089(t) grew at 15-30 °c and ph 5.0-10.0 and tolerated ≤8 % (w/v) nacl. 16s rrna gene sequence analysis showed high pairwise sequence similarity to pseudomonas azotifigens 6h33b(t) (97.3 %) and pseudomonas balearica sp1402(t) (96.7 %) and lower sequence similarity to other strains (<96.0 %). in dna-dna reassocia ...201525525124
the nitrogen-fixation island insertion site is conserved in diazotrophic pseudomonas stutzeri and pseudomonas sp. isolated from distal and close geographical regions.the presence of nitrogen fixers within the genus pseudomonas has been established and so far most isolated strains are phylogenetically affiliated to pseudomonas stutzeri. a gene ortholog neighborhood analysis of the nitrogen fixation island (nfi) in four diazotrophic p. stutzeri strains and pseudomonas azotifigens revealed that all are flanked by genes coding for cobalamin synthase (cobs) and glutathione peroxidise (gshp). the putative nfis lack all the features characterizing a mobilizable gen ...201425251496
draft genome sequence of pseudomonas azotifigens strain dsm 17556t (6h33bt), a nitrogen fixer strain isolated from a compost pile.pseudomonas azotifigens strain 6h33b(t) is a nitrogen fixer isolated from a hyperthermal compost pile in 2005 by hatayama and collaborators. here we report the draft genome, which has an estimated size of 5.0 mb, exhibits an average g+c content of 66.73%, and is predicted to encode 4,536 protein-coding genes and 100 rna genes.201324179119
pseudomonas azotifigens sp. nov., a novel nitrogen-fixing bacterium isolated from a compost pile.a nitrogen-fixing bacterium, designated strain 6h33b(t), was isolated from a compost pile in japan. the nitrogenase activity of this strain was detected based on its acetylene-reducing activity under low oxygen concentrations (2-4%). an analysis of the genes responsible for nitrogen fixation in this strain, nifh and nifd, indicated a close relationship to those of pseudomonas stutzeri a15 (a1501). sequence similarity searches based on the 16s rrna gene sequences showed that strain 6h33b(t) belon ...200516014478
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