| characterization of a novel close-to-root papillomavirus from a florida manatee by using multiply primed rolling-circle amplification: trichechus manatus latirostris papillomavirus type 1. | by using an isothermal multiply primed rolling-circle amplification protocol, the complete genomic dna of a novel papillomavirus was amplified from a skin lesion biopsy of a florida manatee (trichechus manatus latirostris), one of the most endangered marine mammals in united states coastal waters. the nucleotide sequence, genome organization, and phylogenetic position of the trichechus manatus latirostris papillomavirus type 1 (tmpv-1) were determined. tmpv-1 is the first virus isolated from the ... | 2004 | 15507660 |