| anisakis simplex and anisakis physeteris: physicochemical properties of larval and adult hemoglobins. | | 1979 | 573208 |
| the identity of anisakis type ii larvae with anisakis physeteris confirmed by restriction fragment length polymorphism analysis of genomic dna. | the identity of anisakis type ii larvae with adult a. physeteris was confirmed by comparison of restriction fragment length polymorphisms (rflps) of 25s ribosomal dna (rdna). patterns of rflps in larvae were almost identical with those in adult worms. directly labelled 25s rdna might serve as an appropriate probe with highly specific activity for examining rflps of larvae and adult worms. | 1992 | 1361503 |
| [infestation by larvae of anisakis simplex a and anisakis physeteris in fish species of the italian seas]. | data on the occurrence of larvae of anisakis simplex a and anisakis physeteris in marine fishes from italian waters are reported. the larvae have been identified by multilocus electrophoresis using biochemical keys. considerations on the life-history pattern of these species in the mediterranean sea are advanced. | 1989 | 2487892 |
| genetic structure of anisakis physeteris, and its differentiation from the anisakis simplex complex (ascaridida: anisakidae). | the genetic structure of anisakis physeteris from the mediterranean sea has been analysed electrophoretically at 22 enzyme loci. the samples studied, although differing in the life-stage (larvae and adults), and in the host (the fishes micromesistius poutassou and trachurus trachurus, and the sperm whale physeter macrocephalus) were genetically homogeneous. of these loci 11 (ldh, sod, np, adk-2, pgm-1, est-1, est-2, acph-1, acph-2, lap-2 and ca) were found to be monomorphic, while the other 11 ( ... | 1986 | 3785976 |
| anisakis physeteris: amino acids in body tissues. | | 1972 | 5049755 |
| hemoglobin derivatives of the zooparasitic nematode anisakis physeteris and the sperm whale host. | | 1972 | 5057032 |
| occurrence of anisakis physeteris baylis, 1923 and pseudoterranova sp. (nematoda) in pygmy sperm whale kogia breviceps (de blainvillei, 1838) (physeteridae) in northeastern coast of brazil. | | 1998 | 9698890 |
| genetic markers in ribosomal dna for the identification of members of the genus anisakis (nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. | polymerase-chain-reaction-based restriction fragment length polymorphism analysis was performed to establish genetic markers in rdna, for the identification of the three sibling species of the anisakis simplex complex and morphologically differentiated anisakis species, i.e. anisakis physeteris, anisakis schupakovi, anisakis typica and anisakis ziphidarum. different restriction patterns were found between a. simplex sensu stricto and anisakis pegreffii with two of the restriction endonucleases u ... | 2000 | 10704605 |
| genetic divergence and reproductive isolation between anisakis brevispiculata and anisakis physeteris (nematoda: anisakidae)s. | in order to assess the taxonomic status of anisakis brevispiculata dollfus, 1966 population samples of this taxon from central and south-eastern atlantic ocean were compared at 22 enzymatic loci with samples belonging to anisakis physeteris baylis, 1923 from the mediterranean sea and central-eastern atlantic ocean. very low interpopulational genetic divergence was observed both within a. brevispiculata (average d(nei) = 0.008) and within a. physeteris (d(nei) = 0.009) despite the geographic dist ... | 2001 | 11165265 |
| cross-reactivity between antigens of anisakis simplex s.l. and other ascarid nematodes. | a study of the cross-reactivity among somatic and excretory-secretory antigens of the third stage larvae of anisakis simplex s.l. and somatic antigens of other ascarid nematodes (ascaris lumbricoides, a. suum, toxocara canis, anisakis physeteris, hysterothylacium aduncum and h. fabri) was carried out by immunoblotting. it was revealed a high degree of cross-reactivity among ascarids in the 30 and > 212 kda range by using sera against somatic and excretory-secretory antigens of a. simplex s.l. it ... | 2004 | 15224584 |
| [anisakidosis a marine parasitic zoonosis: unknown or emerging in peru?]. | the purpose of this review is to show the experimental studies carried out on the biological cycle, culture, pathogenicity of the anisakidae nematode larvae and to disseminate the information regarding current epidemy and the probable emergence of anisakidosis in peru, and in addition, to propose measures of prevention and control, as well as the perspective and need for investigation. the studies of experimental pathogenicity in cats, dos, and hamsters are incomplete. eight cases of acute human ... | 2004 | 15614302 |
| anisakid parasites of two forkbeards (phycis blennoides and phycis phycis) from the mediterranean coasts of andalucía (southern spain). | an epidemiological survey of anisakids was carried out on 209 specimens of greater forkbeard (phycis blennoides) and 58 of forkbeard (phycis phycis) captured off the mediterranean coasts of andalucía (southern spain). four species of nematodes were identified: anisakis simplex s.l., anisakis physeteris, hysterothylacium aduncum and hysterothylacium fabri. the total prevalence was 62.06% in the forkbeard and 58.85% in the greater forkbeard. the highest values of prevalence (56.90%), mean intensit ... | 2006 | 16168705 |
| molecular characterization of larval anisakid nematodes from marine fishes of madeira by a pcr-based approach, with evidence for a new species. | one-hundred and fifteen anisakid larvae from 3 different fish hosts, aphanopus carbo, scomber japonicus, and trachurus picturatus, caught in madeiran waters, were identified by pcr-rflp. three distinct species were identified in a. carbo, namely anisakis simplex sensu srricto, anisakis pegreffii, and anisakis ziphidarum; 5 in s. japonicus, i.e., a. simplex s.s., a. pegreffii, anisakis physeteris, anisakis typica, and a. ziphidarum; and 3 in t. picturatus, i.e., a. simplex s.s., a. pegreffii, and ... | 2005 | 16539027 |
| anisakid parasites of two forkbeards (phycis blennoides and phycis phycis) from the eastern mediterranean coasts in tunisia. | two gadiform species with a successive bathymetric and an ecological and economical importance in the mediterranean fishing industry, phycis blennoides and phycis phycis, were selected for the present study. a total of 592 fresh specimens belonging to the gadiformes genus were obtained from local commercial fisheries. the investigation was centred on anisakid parasites of 272 specimens of the greater forkbeard (p. blennoides) and 320 of the forkbeard (p. phycis) captured off the mediterranean co ... | 2006 | 16847607 |
| occurrence and molecular identification of anisakis spp. from the north african coasts of mediterranean sea. | larval forms of the genus anisakis were reported infecting several fish species from the north african coasts of central mediterranean sea. polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis was used to investigate the occurrence of larval forms of different anisakis species in teleost fishes and squid from north african coasts of the mediterranean sea and to establish the geographical and host range of these parasites in this area. a total of 282 anisakis lar ... | 2008 | 18026753 |
| application of the pcr-sequence-specific primers for the discrimination among larval anisakis simplex complex. | the fish-borne zoonotic nematode, anisakis simplex, is not a single specie but a complex composed of three sibling species, a. simplex sensu stricto, anisakis pegreffii, and a. simplex c. discrimination among these sibling species have been performed by polymerase chain reaction (pcr)-restriction fragment length polymorphism or sequencing analysis of the internal transcribed spacer (its) region of ribosomal dna (rdna). in the present study, pcr-sequence-specific primers were applied for the disc ... | 2008 | 18239942 |
| parasites in stranded cetaceans of patagonia. | there is an increasing interest in parasites of marine mammals of argentina. here, we examined several poorly known cetaceans, i.e., 2 spectacled porpoises and 1 burmeister's porpoise (phocoenidae), and 1 gray's beaked whale and 1 cuvier's beaked whale (ziphidae); we also updated the parasite information for 1 sperm whale (physeteridae). these hosts strand only occasionally. we found anisakis simplex s.l. in 2 spectacled porpoises and the burmeister's porpoise, and recorded its distribution amon ... | 2008 | 18576713 |
| the role of pelagic swarm fish (myctophidae: teleostei) in the oceanic life cycle of anisakis sibling species at the mid-atlantic ridge, central atlantic. | first information is provided on the parasitation and feeding ecology of the myctophid fish species myctophum punctatum and notoscopelus kroyeri from the mid-atlantic ridge (mar), central atlantic. four different parasite species were found in both fish with a similar high prevalence and intensity of infestation. the digeneans gonocerca phycidis and lethadena sp. were isolated as adults from the stomach, larval tetraphyllidean cestodes (scolex pleuronectis) from the intestine, and genetically id ... | 2008 | 18758825 |
| genetic characterization of anisakis typica and anisakis physeteris from marine mammals and fish from the atlantic ocean off brazil. | until now, anisakis typica has been the sole anisakid identified by means of genetic markers from the atlantic ocean off brazil. in this study we developed, and applied to larvae and adults, an a. typica-specific molecular identification protocol based on the complete intergenic transcribed spacer (its) region. anisakids from the dolphin sotalia guianensis and from the frigate tuna, auxis thazard, caught off the coast of brazil, were processed using two dna extraction methods. molecular diagnosi ... | 2009 | 19651475 |
| multiple primer pcr for the identification of anisakid nematodes from taiwan strait. | there were six major larval anisakid species found in commercial marine fishes caught in the minnan fishing ground in the taiwan strait: anisakis physeteris, anisakis pegreffii, raphidascaris trichiuri, contracaecum aduncum, contracaecum muraenesoxi, contracaecum sp. for rapid identification of the parasite species above, a single and a multiple primer pcr (multiplex pcr) method, using specific primers based on aligned sequences of the internal transcribed spacer its-1, 5.8s, and its-2 of nuclea ... | 2010 | 19751726 |
| molecular characterization and phylogeny of anisakid nematodes from cetaceans from southeastern atlantic coasts of usa, gulf of mexico, and caribbean sea. | in the present study, 407 anisakid nematodes, collected from 11 different species of cetaceans of the families delphinidae, kogiidae, physeteridae, and ziphiidae, from the southeastern atlantic coasts of usa, the gulf of mexico, and the caribbean sea, were examined morphologically and genetically characterized by pcr restriction fragment length polymorphism to identify them to species level, assess their relative frequencies in definitive hosts, and determine any host preference. sequence data f ... | 2011 | 21212982 |
| morphological and molecular characterization of anisakis larvae (nematoda: anisakidae) in beryx splendens from japanese waters. | the third-stage (l3) larvae of anisakis, which are the etiological agents of human anisakiasis, have been categorized morphologically into anisakis type i larvae and anisakis type ii larvae. genetic analysis has allowed easy identification of these larvae: anisakis type i larvae include the species anisakis simplex sensu stricto, anisakis pegreffii, anisakis simplex c, anisakis typica, anisakis ziphidarum, and anisakis nascettii, whereas anisakis type ii larvae include the species anisakis physe ... | 2011 | 21377540 |
| molecular characterization of anisakis larvae from fish caught off sardinia. | abstract anisakis spp. larvae are parasitic, and potentially zoonotic, nematodes transmitted by marine fish and cephalopods, which are the main intermediate hosts of the third larval stage. the accidental consumption of infected raw or poorly cooked fish may cause gastroenteric diseases and allergies in humans. the aim of the present study was to use pcr-rflp to define the occurrence, species variability, and host preferences of anisakis spp. larvae in fish caught off the coast of sardinia. necr ... | 2011 | 21501045 |
| Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters. | The blackspot seabream, Pagellus bogaraveo, is a sparid fish of great economic importance in the northeast Atlantic. The main aim of this work was to assess the infection levels and diversity of anisakid nematodes parasitizing P. bogaraveo from Portuguese waters. The anisakid larvae were identified by polymerase chain reaction-restriction fragment length polymorphism analysis and ten different patterns were observed, four of which were not previously reported in the literature. Moreover, several ... | 2011 | 22139402 |
| scavenging hagfish as a transport host of anisakid nematodes. | hagfish are the most primitive craniates and scavengers, feeding on dead organisms as well as fish and invertebrates. hagfish play an important ecological role in recycling nutrients, helping to recycle biomass from the upper water column. we investigated 265 specimens of four hagfish species, including eptatretus burgeri, eptatretus yangi, eptatretus sheni and eptatretus taiwanae from northeastern taiwanese waters of the northwestern pacific from november 2013 to june 2014. eight species of ani ... | 2016 | 26872923 |
| occurrence and prevalence of fish-borne anisakis larvae in the spotted mackerel scomber australasicus from taiwanese waters. | anisakid nematodes have been found in a variety of marine fishes throughout the world and they are known to cause anisakiasis in human hosts. the present study investigated the prevalence of potentially zoonotic anisakid larvae in spotted mackerel caught from taiwanese waters where fish represents an important food sources. anisakis third-stage larvae (l3, n=502) were isolated from 250 spotted mackerel scomber australasicus. anisakis l3 larvae were divided morphologically into two types, anisaki ... | 2015 | 25733490 |
| effect of different temperatures on the expression of the newly characterized heat shock protein 90 (hsp90) in l3 of anisakis spp. isolated from scomber australasicus. | anisakid nematodes are distributed worldwide in a wide variety of marine fishes and they are known to cause the zoonotic disease, anisakiasis. the temperature control is commonly applied for prevention and control of anisakiasis. to analyze the cellular response to temperature stress in anisakis, the heat shock protein 90 (hsp90) was chosen in the present study, as it plays a key role in many cellular processes and responds to stress conditions such as heat or cold shock. anisakids were sampled ... | 2014 | 25269988 |
| morphological and genetic identification of anisakis paggiae (nematoda: anisakidae) in dwarf sperm whale kogia sima from brazilian waters. | anisakid nematodes have been identified in a wide variety of fish and marine mammal species. in brazil, anisakis physeteris, a. insignis, a. typica, a. nascetti, and those of the a. simplex complex have been reported infecting fishes and cetaceans. in this study, specimens collected from a dwarf sperm whale kogia sima (owen, 1866) stranded on the northeastern coast of brazil were identified through morphological and genetic analyses as a. paggiae. anisakids were examined through differential int ... | 2015 | 25751853 |
| experimental demonstration of pathogenic potential of anisakis physeteris and anisakis paggiae in wistar rats. | anisakis morphotype i is the principal etiologic agent of human anisakiasis, with differences in pathogenicity found between the anisakis simplex s.s. and a. pegreffii species; however, the role of morphotype ii larvae in this illness is not well understood. the purpose of this study is to verify the ability of morphotype ii larvae to invade tissues via the experimental infection of wistar rats, an animal model which simulates infection in humans. in the in vivo assay, 7.1% (4/56 l3 morphotype i ... | 2014 | 25240961 |
| three anisakis spp. isolated from toothed whales stranded along the eastern adriatic sea coast. | knowledge concerning cetacean ecology in the mediterranean is limited but important for sustainable planning and enforcement of appropriate conservation measures. any information that might help to elucidate their ecology is essential. we explored the population and genetic structures of anisakis spp. nematodes isolated from four toothed whale species - bottlenose dolphins (tursiops truncatus), striped dolphins (stenella coeruleoalba), risso's dolphins (grampus griseus) and cuvier's beaked whale ... | 2015 | 25449948 |
| molecular and epidemiological data on anisakis spp. (nematoda: anisakidae) in commercial fish caught off northern sardinia (western mediterranean sea). | anisakiasis is a fish-borne zoonosis caused by third stage larvae of the nematode anisakis sp. present in fish or cephalopods. this is the first contribution to the molecular identification and epidemiology of anisakis spp. in commercial fish from the gulf of asinara (sardinia, western mediterranean sea). between april 2006 to november 2011, 777 specimens of 10 fish species (engraulis encrasicolus, merluccius merluccius, micromesistius poutassou, phycis blennoides, sardina pilchardus, sardinella ... | 2014 | 24630706 |
| molecular identification of anisakis spp. from fishes collected in the tyrrhenian sea (nw mediterranean). | the accurate identification of anisakid nematodes at any life cycle stage is important both to deepen the knowledge on their taxonomy, ecology, epidemiology and for diagnosis and control, as larval stages cause a clinical disease in humans known as anisakidosis. with the aim to investigate the presence of anisakid larvae, specimens of horse mackerel, trachurus trachurus (linnaeus, 1758), silver scabbardfish, lepidopus caudatus (euphrasen, 1788), european anchovy, engraulis encrasicolus (linnaeus ... | 2012 | 22360842 |
| anisakis spp. larvae in three mesopelagic and bathypelagic fish species of the central mediterranean sea. | in this work 437 fish samples of species belonging to the families myctophidae (electrona risso and diaphus metopoclampus) and phosichthyidae (vinciguerria attenuata) were examined for the presence of anisakidae larvae. the study was performed with fishes in the central mediterranean sea, particularly in the strait of sicily and in the strait of messina. the visual inspection and chloro-peptic analysis revealed the presence of nematode parasites with prevalence values between 2.9% in electrona r ... | 2018 | 28965943 |