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advenella incenata gen. nov., sp. nov., a novel member of the alcaligenaceae, isolated from various clinical samples.a polyphasic taxonomic study of 14 isolates recovered from various human and veterinary clinical samples was performed. phenotypically these isolates shared several characteristics with members of the alcaligenaceae and related genera. random amplified polymorphic dna fingerprinting and whole-cell protein analysis suggested the presence of multiple genomic groups, which was confirmed by dna-dna hybridization experiments. 16s rrna gene sequence analysis indicated that these isolates were related ...200515653883
reclassification of the members of the genus tetrathiobacter ghosh et al. 2005 to the genus advenella coenye et al. 2005.the taxonomic position of the genera advenella and tetrathiobacter was examined. 16s rrna gene sequence analysis revealed that the two genera are closely related, representing a monophyletic cluster with high sequence similarity (98.1-99.7%) within the family alcaligenaceae. the phenotypic characteristics of the type strains of advenella incenata, tetrathiobacter kashmirensis and tetrathiobacter mimigardefordensis were re-examined using the api 20ne, api zym and api 50ch systems. phylogenetic da ...200919567588
the role of a groundwater bacterial community in the degradation of the herbicide terbuthylazine.a bacterial community in an aquifer contaminated by s-triazines was studied. groundwater microcosms were treated with terbuthylazine at a concentration of 100 microg l(-1) and degradation of the herbicide was assessed. the bacterial community structure (abundance and phylogenetic composition) and function (carbon production and cell viability) were analysed. the bacterial community was able to degrade the terbuthylazine; in particular, betaproteobacteria were involved in the herbicide biotransfo ...201019840114
16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens.a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ...200919937215
dihydrolipoamide dehydrogenases of advenella mimigardefordensis and ralstonia eutropha catalyze cleavage of 3,3'-dithiodipropionic acid into 3-mercaptopropionic acid.the catabolism of the disulfide 3,3'-dithiodipropionic acid (dtdp) is initiated by the reduction of its disulfide bond. three independent tn5::mob-induced mutants of advenella mimigardefordensis strain dpn7(t) were isolated that had lost the ability to utilize dtdp as the sole source of carbon and energy and that harbored the transposon insertions in three different sites of the same dihydrolipoamide dehydrogenase gene encoding the e3 subunit of the pyruvate dehydrogenase multi-enzyme complex of ...201020833784
a type ib plasmid segregation machinery of the advenella kashmirensis plasmid pbtk445.pbtk445 is a newly described large (∼60kb), low-copy number, conjugative plasmid indigenous to the sulfur-chemolithoautotroph advenella kashmirensis. based on its minimal replication region, a shuttle vector, pbtks was constructed which can be used for diverse alcaligenaceae members. the construct was found to be stably maintained both in the native host as well as in escherichia coli in the absence of selective pressure which indicated that pbtks harbors the stabilizing system of pbtk445, that ...201021192970
novel reaction of succinyl coenzyme a (succinyl-coa) synthetase: activation of 3-sulfinopropionate to 3-sulfinopropionyl-coa in advenella mimigardefordensis strain dpn7t during degradation of 3,3'-dithiodipropionic acid.the succd gene of advenella mimigardefordensis strain dpn7(t) encodes a succinyl coenzyme a (succinyl-coa) synthetase homologue (ec 6.2.1.4 or ec 6.2.1.5) that recognizes, in addition to succinate, the structural analogues 3-sulfinopropionate (3sp) and itaconate as substrates. accumulation of 3sp during 3,3'-dithiodipropionic acid (dtdp) degradation was observed in tn5::mob-induced mutants of a. mimigardefordensis strain dpn7(t) disrupted in succd and in the defined deletion mutant a. mimigardef ...201121515777
genetic diversity and phylogeny of antagonistic bacteria against phytophthora nicotianae isolated from tobacco rhizosphere.the genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by boxair-pcr, 16s-rflp, 16s rrna sequence homology and phylogenetic analysis methods. these studies revealed that 4.01% of the 6652 tested had some inhibitory activity against phytophthora nicotianae. boxair-pcr analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. a total of 25 16s-rflp patterns were iden ...201121686169
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