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description of four novel species of xenorhabdus, family enterobacteriaceae: xenorhabdus budapestensis sp. nov., xenorhabdus ehlersii sp. nov., xenorhabdus innexi sp. nov., and xenorhabdus szentirmaii sp. nov.the taxonomic affiliation was determined for four xenorhabdus strains isolated from four steinernema hosts from different countries. as compared to the five validly described xenorhabdus species, i.e., x. nematophila, x. japonica, x. beddingii, x. bovienii and x. poinarii, these isolates represented novel species on the basis of 16s rrna gene sequences and riboprint patterns, as well as by physiological and metabolic properties. they were named xenorhabdus budapestensis sp. nov., type strain dsm ...200515830803
xenorhabdus thuongxuanensis sp. nov. and xenorhabdus eapokensis sp. nov., isolated from steinernema species.two slightly yellowish pigmented, oxidase-negative, rod-shaped and gram-negative bacterial strains (30tx1t and dl20t), isolated from steinernema sangi and steinernema eapokense, respectively, during soil sampling in vietnam were studied in a polyphasic taxonomic approach. strain 30tx1t showed highest 16s rrna gene sequence similarity to xenorhabdus ehlersii (98.9%) and strain dl20t to xenorhabdus ishibashii (98.7%). sequence similarities to all other xenorhabdus species were lower (< 98.4%). the ...201728056225
an insecticidal protein from xenorhabdus ehlersii stimulates the innate immune response in galleria mellonella.the bacteria xenorhabdus spp. are entomopathogenic symbionts that can produce several toxic proteins that interfere with the immune system of insects. recently, we purified the insecticidal protein xegroel from xenorhabdus ehlersii and discovered that injection of xegroel into larvae of galleria mellonella triggers strong immune responses. in this study, we determined the level of induction of several immune-responsive proteins that were secreted into the hemolymph using comparative proteomic an ...201323529358
xenorhabdus ishibashii sp. nov., isolated from the entomopathogenic nematode steinernema aciari.gram-negative bacteria of the genus xenorhabdus exhibit a mutualistic association with steinernematid entomopathogenic nematodes and a pathogenic relationship with insects. here we describe two isolates of the entomopathogenic nematode steinernema aciari collected from china and japan. 16s rrna gene sequence similarity and phylogenetic analysis indicated that the isolates obtained from s. aciari belonged to the genus xenorhabdus. multilocus sequence analysis based on five universal protein-codin ...201322922533
an insecticidal protein from xenorhabdus ehlersii triggers prophenoloxidase activation and hemocyte decrease in galleria mellonella.the bacteria xenorhabdus spp. are entomopathogenic symbionts that can produce several toxic proteins that interfere the immune system of insects. we purified an insecticidal protein from xenorhabdus ehlersii, and designated it as xegroel with an estimated molecular mass of ~58 kda. galleria mellonella larva injected with xegroel presented prophenoloxidase activation and hemocyte decrease. xegroel can kill g. mellonella larva in 48 h with an ld(50) of 0.76 ± 0.08 μg/larva. our results demonstrate ...201222477033
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