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a new black aspergillus species, a. vadensis, is a promising host for homologous and heterologous protein production.a new species of the group of black aspergilli, aspergillus vadensis, was analyzed for its potential as a host for homologous and heterologous protein production. unlike the other black aspergilli, this strain does not acidify the culture medium when nitrate is the nitrogen source and only produces very low levels of extracellular proteases, mainly serine metalloproteases. the stability of a. tubingensis feruloyl esterase a (faea) was compared upon production in wild-type a. vadensis, a. tubinge ...200415240269
aspergillus vadensis, a new species of the group of black aspergilli.a strain from the group of black aspergilli was analysed in detail to determine the species to which it belongs. a detailed analysis of morphology, rflp patterns and metabolite profiles was carried out. in addition, a phylogenetic tree was constructed for the black aspergilli using the its and the beta-tubulin sequences of the individual strains. the new species differs by its poor growth on glycerol and galacturonate and its unique extrolite profile consisting of aurasperone b, nigragillin, asp ...200515803385
two glucuronoyl esterases of phanerochaete chrysosporium.the white-rot fungus phanerochaete chrysosporium produces glucuronoyl esterase, a recently discovered carbohydrate esterase, during growth on sugar beet pulp. two putative genes encoding this enzyme, ge1 and ge2, were isolated and cloned. heterologous expression in aspergillus vadensis, pycnoporus cinnabarinus and schizophyllum commune resulted in extracellular glucuronoyl esterase activity, demonstrating that these genes encode this enzymatic function. the amino acid sequence of ge1 was used to ...200918854978
technical advance in fungal biotechnology: development of a miniaturized culture method and an automated high-throughput screening.the goal of the study was to develop a reliable, reproducible and rapid method of culture in order to screen a large number of fungal transformants.200919486280
Aspergillus niger contains the cryptic phylogenetic species A. awamori.Aspergillus section Nigri is an important group of species for food and medical mycology, and biotechnology. The Aspergillus niger 'aggregate' represents its most complicated taxonomic subgroup containing eight morphologically indistinguishable taxa: A. niger, Aspergillus tubingensis, Aspergillus acidus, Aspergillus brasiliensis, Aspergillus costaricaensis, Aspergillus lacticoffeatus, Aspergillus piperis, and Aspergillus vadensis. Aspergillus awamori, first described by Nakazawa, has been compar ...201122036292
overexpression, purification and characterisation of homologous α-l-arabinofuranosidase and endo-1,4-β-d-glucanase in aspergillus vadensis.in the recent past, much research has been applied to the development of aspergillus, most notably a. niger and a. oryzae, as hosts for recombinant protein production. in this study, the potential of another species, aspergillus vadensis, was examined. the full length gdna encoding two plant biomass degrading enzymes, i.e. α-l-arabinofuranosidase (abfb) (gh54) and endo-1,4-β-d-glucanase (egla) (gh12) from a. vadensis were successfully expressed using the gpda promoter from a. vadensis. both enzy ...201425260653
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