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kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (kordiimonadales ord. nov.) in the 'alphaproteobacteria'.a marine bacterium, designated strain gw14-5(t), capable of degrading high-molecular-mass polycyclic aromatic hydrocarbons was isolated from the sediments of gwangyang bay, republic of korea, after enrichment culture for 2 years with a mixture of benzo[a]pyrene and pyrene. phylogenetic analysis based on 16s rrna gene sequences indicated that the isolate forms a phyletic lineage that is distinct from the seven known orders within the 'alphaproteobacteria'. 16s rrna gene sequence similarity of str ...200516166706
novel marine carbazole-degrading bacteria.eleven carbazole (car)-degrading bacterial strains were isolated from seawater collected off the coast of japan using two different media. seven isolates were shown to be most closely related to the genera erythrobacter, hyphomonas, sphingosinicella, caulobacter, and lysobacter. meanwhile, strains oc3, oc6s, oc9, and oc11s showed low similarity to known bacteria, the closest relative being kordiimonas gwangyangensis gw14-5 (90% similarity). southern hybridization analysis revealed that only five ...200919187209
gene diversity of cyp153a and alkb alkane hydroxylases in oil-degrading bacteria isolated from the atlantic ocean.alkane hydroxylases, including the integral-membrane non-haem iron monooxygenase (alkb) and cytochrome p450 cyp153 family, are key enzymes in bacterial alkane oxidation. although both genes have been detected in a number of bacteria and environments, knowledge about the diversity of these genes in marine alkane-degrading bacteria is still limited, especially in pelagic areas. in this report, 177 bacterial isolates, comprising 43 genera, were obtained from 18 oil-degrading consortia enriched from ...201020148932
kordiimonas lacus sp. nov., isolated from a ballast water tank, and emended description of the genus kordiimonas.a gram-negative, motile, rod-shaped bacterial strain, designated s3-22(t), was isolated from a sediment sample collected from a ballast water tank of a commercial ship and subjected to a polyphasic taxonomic characterization. the isolate formed small, light-yellow, semi-translucent and circular colonies on solid complex media. the strain was oxidase- and catalase-positive and metabolized a large number of carbon sources. chemotaxonomic analysis showed ubiquinone q-10 as predominant respiratory q ...201120348321
isolation and characterization of the gene encoding the chloroplast-type ferredoxin component of carbazole 1,9a-dioxygenase from a putative kordiimonas sp.carbazole (car)-degrading genes (carraacbabb) were isolated from marine car-degrading isolate strain oc9 (probably kordiimonas gwangyangensis) using shotgun cloning experiments and showed 35-65% similarity with previously reported car-degrading genes. in addition, a ferredoxin-like gene (carac) was found downstream of carr, although it was not homologous with any reported ferredoxin components of the car 1,9a-dioxygenase (cardo) system. the carac-deduced amino acid sequence possessed consensus s ...201020652619
genetic characterisation of genes involved in the upper pathway of carbazole metabolism from the putative kordiimonas sp.the car genes from a carbazole (car)-degrading bacterium, kordiimonas sp. oc9, were functionally and transcriptionally analysed. the enzymatic activity for the protein coded by carbabb using pboc93 (caraaacba), pboc93-2 (caraaacbb), and pboc94 (caraaacbabb) was confirmed. resting cells using escherichia coli harbouring pboc95 (caraaacbabbc) revealed the function of the carc gene product in the conversion of car to anthranilic acid by expressing it with caraaacbabb. the pathway of car metabolism ...201121553289
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