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self-assembled glucosamine monolayers as biomimetic receptors for detecting wga lectin and influenza virus with a quartz crystal microbalance.n-acetylglucosamine (glcnac) is a natural ligand that interacts with the binding sites of wheat germ agglutinin (wga) lectin. for immobilization, glcnac was linked to p-nitrophenol, and the nitro group was reduced and then bound to cysteine via two-step synthesis. scanning tunneling microscopy studies revealed proper immobilization of the ligand on the gold surface of a quartz crystal microbalance (qcm) via the cysteine s-h bond as well as binding between glcnac and wga. qcm measurements reveale ...201323715677
genetically diverse low pathogenicity avian influenza a virus subtypes co-circulate among poultry in bangladesh.influenza virus surveillance, poultry outbreak investigations and genomic sequencing were assessed to understand the ecology and evolution of low pathogenicity avian influenza (lpai) a viruses in bangladesh from 2007 to 2013. we analyzed 506 avian specimens collected from poultry in live bird markets and backyard flocks to identify influenza a viruses. virus isolation-positive specimens (n = 50) were subtyped and their coding-complete genomes were sequenced. the most frequently identified subtyp ...201627010791
a novel reassortant h2n3 influenza virus isolated from china.to analyze the genetic composition of a novel h2n3 virus isolate identified from a duck cage swab in a live poultry market (lpm) in 2009 in guangdong province of china.201424758752
genetic analysis of avian influenza viruses: cocirculation of avian influenza viruses with allele a and b nonstructural gene in northern pintail (anas acuta) ducks wintering in japan.the pandemic influenza virus strains of 1918 (h1n1), 1957 (h2n2), 1968 (h3n2), and 2009 (h1n1) have genes related to avian influenza viruses (aivs). the nonstructural (ns) gene of aivs plays a significant role in host-viral interaction. however, little is known about the degree of diversity of this gene in northern pintail (anas acuta) ducks wintering in japan. this study describes characteristics of pintail-originated h1n1, h1n2, h1n3, h5n2, h5n3, h5n9, and h7n7 viruses. most of the viruses wer ...201223320157
live bird markets of bangladesh: h9n2 viruses and the near absence of highly pathogenic h5n1 influenza.avian influenza surveillance in bangladesh has been passive, relying on poultry farmers to report suspected outbreaks of highly pathogenic h5n1 influenza. here, the results of an active surveillance effort focusing on the live-bird markets are presented. prevalence of influenza infection in the birds of the live bird markets is 23.0%, which is similar to that in poultry markets in other countries. nearly all of the isolates (94%) were of the non-pathogenic h9n2 subtype, but viruses of the h1n2, ...201121541296
the isolation of influenza a viruses and paramyxoviruses from feral ducks in new zealand.tracheal and cloacal swabs from apparently healthy ducks, gulls, shearwaters and terns in new zealand were tested for myxoviruses by inoculation into embryonated eggs. seven influenza a viruses belonging to three antigenic subtypes (h4n6; h1n3; h11n3) and nine paramyxoviruses of two antigenic subtypes (pmv-1; pmv-4) were isolated from feral ducks. the occurrence of the same virus subtypes in birds, including ducks, in other countries suggests that they were introduced into new zealand by the imp ...19846497784
isolation of a natural recombinant of influenza a virus (h1n3) from a sick child. 19836139585
[physicochemical properties of the rna and proteins of an influenza virus h1n3 isolated from an ill child and antigenically analogous to a/whale/to/19/76].a comparative analysis of rna and proteins of influenza a/baku/799/82, a/whale/to/19/76, and a/pr8/34 viruses was carried out. the viruses were shown to be similar in their polypeptide composition and oligopeptide maps of the heavy (ha1) and light (ha2) chains of hemagglutinin; in their migration properties of rna fragments in polyacrylamide gel the a/baku/799/82 and a/whale/to/19/76 viruses were similar but not identical. marked differences in the electrophoretic mobility in gel of rna fragment ...19854002690
isolation of influenza a viruses from migratory waterfowls in san-in district, western japan in winters of 1980-1982.in the two winters of 1980-1982, we surveyed migratory waterfowl of some species staying in san-in district, western japan for influenza virus at a few stations. from november 1980 to april 1981, only two strains of influenza virus, h13n1 and h11n6 subtypes, were isolated from 465 fecal samples from pintails but none from 255 samples from whistling swans nor from 625 black-tailed gulls. from november 1981 to march 1982, 17 viruses were isolated from 1156 fecal samples. fourteen viruses, 10 h7n3, ...19873673333
[intracellular proteolytic cleavage of the influenza virus protein np as a sign of the epidemicity of virus strains?].the main nucleocapsid protein (np) of human epidemic viruses was found to be cleaved via np56----hp53 mol. wt. reduction in infected cells, while the np of animal influenza viruses was refractory to analogous intracellular modification. like animal influenza viruses, the strain a/baku/799/82(h1n3) isolated from a sick child has been observed to exhibit the intracellular resistance of np to intracellular proteolysis. the similar np resistance has been revealed for a/new jersey/8/76(h1n1) and a/se ...19873553909
genetic characterization of influenza a virus subtypes h1n3 and h1n9 isolated from free-grazing ducks in thailand.influenza a virus (iav) subtype h1 has been reported to infect birds, pigs and humans. in this study, we characterized iavs subtype h1n3 and h1n9 isolated from free-grazing ducks in thailand. phylogenetic analysis showed that thai iav-h1 isolates cluster with avian eurasian-lineage but not pandemic h1n1 viruses. analysis of the viruses indicated low-pathogenic avian influenza (lpai) characteristics. this study is the first report of avian h1n3 and h1n9 in thailand. although thai iav-h1 viruses d ...201627383209
complete genome sequences of a novel reassortant h4n2 avian influenza virus isolated from a live poultry market in eastern china.a/duck/shanghai/28-1/2009(h4n2) (dk28) was isolated from a live poultry market in shanghai, china. using pcr and sequencing analysis, we obtained the complete genome sequences of the dk28 virus. the sequence analysis demonstrated that this h4n2 virus was a novel multiple-gene reassortant avian influenza virus (aiv) whose genes originated from h1n1, h1n3, h3n3, h4n2, and h4n6. knowledge regarding the complete genome sequences of the dk28 virus will be useful for epidemiological surveillance.201223043180
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