| engineered bacillus thuringiensis go33a with broad insecticidal activity against lepidopteran and coleopteran pests. | a recombinant plasmid pstk-3a containing cry3aa7 gene encoding a coleopteran-specific insecticidal protein was constructed and introduced into wild bacillus thuringiensis subsp. aizawai g03, which contained cry1aa, cry1ac, cry1ca, and cry2ab genes and was highly toxic to lepidopteran insect pests. the genetically engineered strain were named g033a. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot analysis demonstrated that the cry3aa7 gene was expressed normally and pro ... | 2006 | 16572346 |
| identification of cry1i-type genes from bacillus thuringiensis strains and characterization of a novel cry1i-type gene. | a pcr-restriction fragment length polymorphism method for identification of cry1i-type genes from bacillus thuringiensis was established by designing a pair of universal primers based on the conserved regions of the genes to amplify 1,548-bp cry1i-type gene fragments. amplification products were digested with the bsp119i and bani enzymes, and four kinds of known cry1i-type genes were successfully identified. the results showed that cry1i-type genes appeared in 95 of 115 b. thuringiensis isolates ... | 2003 | 12957903 |