| members of the cytophaga-flavobacterium-bacteroides phylum as intracellular bacteria of acanthamoebae: proposal of 'candidatus amoebophilus asiaticus'. | three gram-negative, rod-shaped bacteria that were found intracellularly in two environmental and one clinical acanthamoeba sp. isolates were analysed. two endocytobionts showing a parasitic behaviour were propagated successfully outside their amoebal host cells and were identified subsequently by comparative 16s rrna sequence analysis as being most closely affiliated with flavobacterium succinicans (99% 16s rrna sequence similarity) or flavobacterium johnsoniae (98% 16s rrna sequence similarity ... | 2001 | 11553234 |
| characterization of a 'bacteroidetes' symbiont in encarsia wasps (hymenoptera: aphelinidae): proposal of 'candidatus cardinium hertigii'. | previously, analysis of 16s rdna sequences placed a newly discovered lineage of bacterial symbionts of arthropods in the 'bacteroidetes'. this symbiont lineage is associated with a number of diverse host reproductive manipulations, including induction of parthenogenesis in several encarsia parasitoid wasps (hymenoptera: aphelinidae). in this study, electron microscopy and phylogenetic analysis of the 16s rrna and gyrb genes of symbionts from encarsia hispida and encarsia pergandiella are used to ... | 2004 | 15143050 |
| endosymbionts of acanthamoeba isolated from domestic tap water in korea. | in a previous study, we reported our discovery of acanthamoeba contamination in domestic tap water; in that study, we determined that some acanthamoeba strains harbor endosymbiotic bacteria, via our molecular characterization by mitochondrial dna restriction fragment length polymorphism (mt dna rflp). five (29.4%) among 17 acanthamoeba isolates contained endosymbionts in their cytoplasm, as demonstrated via orcein staining. in order to estimate their pathogenicity, we conducted a genetic charact ... | 2009 | 19967080 |
| the genome of the amoeba symbiont "candidatus amoebophilus asiaticus" reveals common mechanisms for host cell interaction among amoeba-associated bacteria. | protozoa play host for many intracellular bacteria and are important for the adaptation of pathogenic bacteria to eukaryotic cells. we analyzed the genome sequence of "candidatus amoebophilus asiaticus," an obligate intracellular amoeba symbiont belonging to the bacteroidetes. the genome has a size of 1.89 mbp, encodes 1,557 proteins, and shows massive proliferation of is elements (24% of all genes), although the genome seems to be evolutionarily relatively stable. the genome does not encode pat ... | 2010 | 20023027 |
| genome analysis of microorganisms living in amoebae reveals a melting pot of evolution. | abstract amoebae-resistant microorganisms exhibit a specific lifestyle. unlike allopatric specialized intracellular pathogens, they have not specialized because they infect the amoebae via amoebal attack and present a sympatric lifestyle with species from different phyla. in this review, we compare the genomes from bacteria (legionella pneumophila, legionella drancourtii, candidatus'protochlamydia amoebophila,'rickettsia bellii, candidatus'amoebophilus asiaticus') and a virus (mimivirus) that mu ... | 2010 | 20132312 |
| the genome of the amoeba symbiont "candidatus amoebophilus asiaticus" encodes an afp-like prophage possibly used for protein secretion. | the recently sequenced genome of the obligate intracellular amoeba symbiont 'candidatus amoebophilus asiaticus' is unique among prokaryotic genomes due to its extremely large fraction of genes encoding proteins harboring eukaryotic domains such as ankyrin-repeats, tpr/sel1 repeats, leucine-rich repeats, as well as f- and u-box domains, most of which likely serve in the interaction with the amoeba host. here we provide evidence for the presence of additional proteins which are presumably presente ... | 2010 | 21178499 |
| two new subfamilies of dna mismatch repair proteins (muts) specifically abundant in the marine environment. | muts proteins are ubiquitous in cellular organisms and have important roles in dna mismatch repair or recombination. in the virus world, the amoeba-infecting mimivirus, as well as the recently sequenced cafeteria roenbergensis virus are known to encode a muts related to the homologs found in octocorals and ɛ-proteobacteria. to explore the presence of muts proteins in other viral genomes, we performed a genomic survey of four giant viruses ('giruses') (pyramimonas orientalis virus (pov), phaeocys ... | 2011 | 21248859 |
| [sequence analysis of 16s rdna gene of endosymbiont of acanthamoeba sp. cb/s1 isolated from soil]. | the endosymbiont of acantlhamoeba sp. cb/si was identified by orcein-carmine staining and 16s rdna sequence analysis. the endosymbiont bacteria were rod-shaped and darkly stained, and irregularly localized within the cytoplasm. the length of the 16s rdna was 1534 bp and its dna sequence was closely related to those of candidatus amoebophilus asioticus and acanthamoeba sp. ka/e21 with 98% homology. phylogenetic analysis showed that the endosymbiont of cb/si, the endosymbiont of ka/e21, candidatus ... | 2011 | 21826891 |
| a bacterial genome in transition--an exceptional enrichment of is elements but lack of evidence for recent transposition in the symbiont amoebophilus asiaticus. | insertion sequence (is) elements are important mediators of genome plasticity and are widespread among bacterial and archaeal genomes. the 1.88 mbp genome of the obligate intracellular amoeba symbiont amoebophilus asiaticus contains an unusually large number of transposase genes (n = 354; 23% of all genes). | 2011 | 21943072 |