| new records of endoparasites infecting hypsiboas albopunctatus (anura: hylidae) in a savanna area in brasília, brazil. | seventy specimens of hypsiboas albopunctatus (anura: hylidae) were collected and surveyed for endoparasites in brasília, brazil at the end of the rainy season (february and march) in 2005. sixteen males (22.86%) were infected by rhabdias sp., aplectana sp., cosmocerca sp., and falcaustra mascula. rhabdias sp., a lung parasite, presented the highest prevalence (8.57%), mean intensity of infestation (1.333), and mean abundance (0.114). moreover, this is the first report of a rhabdiasid nematode in ... | 2008 | 18058130 |
| hylin a1, the first cytolytic peptide isolated from the arboreal south american frog hypsiboas albopunctatus ("spotted treefrog"). | rp-hplc fractionation of the electrically stimulated skin secretion of the arboreal south american frog hypsiboas albopunctatus ("spotted treefrog") led to the isolation of a cytolytic c-terminally amidated peptide. this novel peptide, named hylin a1 (hy-a1), consists of 18 amino acid residues (ifgailplalgalknlik-nh(2)). the alpha-helical structure of the synthetic hylin a1 peptide was confirmed by cd spectroscopy in the presence of 60% (v/v) tfe. the synthetic peptide displayed broad-spectrum a ... | 2009 | 19056441 |
| hemoparasites of the genus trypanosoma (kinetoplastida: trypanosomatidae) and hemogregarines in anurans of the são paulo and mato grosso do sul states - brazil. | wild animals are exposed to numerous pathogens, including hemoparasites. the trypanosoma and hemogregarinegroup are frequently reported as parasites in anurans (frogs, tree frogs and toads). the identification of these hemoparasites is usually made through stage observation of their morphology in the peripheral blood of the host. there areno studies, however, based on the biological cycle of these hemoparasites. the objective of the present study was toevaluate the presence of hemogregarines and ... | 2009 | 19488624 |
| influence of n-terminus modifications on the biological activity, membrane interaction, and secondary structure of the antimicrobial peptide hylin-a1. | recently the peptide hy-a1 (ifgailplalgalknlik), with antimicrobial activity, was isolated from the skin secretion of the frog hypsiboas albopunctatus. the aim of the present work was to evaluate four analogues with introduction of acetyl group, asp or lys at the n-terminus of antimicrobial peptide hy-al to supply information about the relationship of structure-biological activity. the antimicrobial activities were assayed by measuring growth inhibition of four species of bacteria and four speci ... | 2011 | 20560142 |
| antimicrobial activity of the synthetic peptide lys-a1 against oral streptococci. | the peptide lys-[trp(6)]-hy-a1 (lys-a1) is a synthetic derivative of the peptide hy-a1, initially isolated from the frog species hypsiboas albopunctatus. according to previous research, it is a molecule with broad antimicrobial activity. the objective of this study was to evaluate the antimicrobial activity of the synthetic peptide lys-a1 (kifgaiwplalgalknlik-nh2) on the planktonic and biofilm growth of oral bacteria. the methods used to evaluate antimicrobial activity include the following: det ... | 2013 | 23340019 |
| possible interspecific origin of the b chromosome of hypsiboas albopunctatus (spix, 1824) (anura, hylidae), revealed by microdissection, chromosome painting, and reverse hybridisation. | the b chromosome in the hylid hypsiboas albopunctatus (2n = 22 + b) is small, almost entirely composed of c-positive heterochromatin, and does not pair with any chromosome of the a complement. b probe, obtained by microdissection and dop-pcr amplification, was used to search for homology between the b and regular chromosomes of h. albopunctatus and of the related species h. raniceps (cope, 1862). reverse hybridisation was also carried out in the investigation. the b probe exclusively painted the ... | 2014 | 25349670 |
| mechanism of action and relationship between structure and biological activity of ctx-ha: a new ceratotoxin-like peptide from hypsiboas albopunctatus. | the increase in bacterial resistance to current antibiotics has led to the development of new active molecules. we have isolated the antimicrobial peptide ctx-ha from the skin secretion of the frog hypsiboas albopunctatus. the aim of the present work was to elucidate the mechanism of action of this new antimicrobial peptide. the sequence similarity with ceratotoxin, the pore size, and the pore-like release of carboxyfluorescein from vesicles indicated that ctx(ile21)-ha has a mechanism of action ... | 2012 | 22519531 |
| chromosome banding in three species of hypsiboas (hylidae, hylinae), with special reference to a new case of b-chromosome in anuran frogs and to the reduction of the diploid number of 2n = 24 to 2n = 22 in the genus. | the chromosomes of hylids hypsiboas albopunctatus, h. raniceps, and h. crepitans from brazil were analyzed with standard and differential staining techniques. the former species presented 2n = 22 and 2n = 23 karyotypes, the odd diploid number is due to the presence of an extra element interpreted as b chromosome. although morphologically very similar to the small-sized chromosomes of the a complement, the b was promptly recognized, even under standard staining, on the basis of some characteristi ... | 2007 | 17031494 |
| calling rate, corticosterone plasma levels and immunocompetence of hypsiboas albopunctatus. | during the breeding season, male anuran amphibians produce advertisement calls. androgens play a permissive role in the activation of calling activity, which is often positively correlated to androgen plasma levels and testes mass. additionally, calling effort is also correlated to corticosterone plasma levels (hereinafter referred to as cort), which is associated with the mobilization of energy substrates to sustain the high energy flux associated with this activity. however, high cort also has ... | 2016 | 27364933 |
| micelle bound structure and model membrane interaction studies of the peptide hylin a1 from the arboreal south american frog hypsiboas albopunctatus. | antimicrobial peptides (amps) appear as a promising therapeutic candidate against multiresistant pathogens, because they are able to kill microorganisms and have low toxicity of resistance cells. hylin a1 (hy-a1, ifgailplalgalknlik-nh2) is a peptide extracted from the skin secretion of the frog hypsiboas albopunctatus, which displays antimicrobial and hemolytic activities. we report here structural studies of hy-a1 using different techniques such as fluorescence, cd and nmr. our data showed that ... | 2015 | 26059694 |
| dynamics and conformational studies of toac spin labeled analogues of ctx(ile(21))-ha peptide from hypsiboas albopunctatus. | antimicrobial peptides (amps) isolated from several organisms have been receiving much attention due to some specific features that allow them to interact with, bind to, and disrupt cell membranes. the aim of this paper was to study the interactions between a membrane mimetic and the cationic amp ctx(ile(21))-ha as well as analogues containing the paramagnetic amino acid 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (toac) incorporated at residue positions n = 0, 2, and 13. circ ... | 2013 | 23585852 |
| cryptic lineages and pleistocene population expansion in a brazilian cerrado frog. | diversification of south american species endemic to open habitats has been attributed to both tertiary events and pleistocene climatic fluctuations. nonetheless, phylogeographical studies of taxa in these regions are few, precluding generalizations about the timing and processes leading to differentiation and speciation. we inferred population structure of hypsiboas albopunctatus, a frog widely distributed in the brazilian cerrado. three geographically distinct lineages were recovered in our ph ... | 2012 | 22211375 |