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near-full-length genome sequence of a novel reovirus from the chinese mitten crab, eriocheir sinensis.a novel eriocheir sinensis reovirus (esrv) was identified using deep-sequencing techniques in crabs afflicted with trembling disease (td). near-full-length genome sequences of 12 segments of esrv were obtained. the genome of esrv will facilitate further studies on the causative agent of td.201525999569
detecting a novel eriocheir sinensis reovirus by reverse transcription loop-mediated isothermal amplification assay.the novel eriocheir sinensis reovirus (esrv) is a pathogen that causes severe disease and high mortality rates in cultivated crabs. here, we established a highly sensitive and specific rapid reverse transcription loop-mediated isothermal amplification (rt-lamp) assay that was cheaper and more suitable for field applications in crab aquaculture than those of traditional reverse transcription-polymerase chain reaction (rt-pcr) analysis. the amplification was completed within 45 min under isotherma ...201627495940
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