detecting a novel eriocheir sinensis reovirus by reverse transcription loop-mediated isothermal amplification assay. | the novel eriocheir sinensis reovirus (esrv) is a pathogen that causes severe disease and high mortality rates in cultivated crabs. here, we established a highly sensitive and specific rapid reverse transcription loop-mediated isothermal amplification (rt-lamp) assay that was cheaper and more suitable for field applications in crab aquaculture than those of traditional reverse transcription-polymerase chain reaction (rt-pcr) analysis. the amplification was completed within 45 min under isotherma ... | 2016 | 27495940 |