| biochemical characterization of c4 protein of cotton leaf curl kokhran virus-dabawali. | cotton leaf curl kokhran virus-dabawali (clcukv-dab) is a monopartite begomovirus encoding two proteins v1 and v2 in the virion sense and four proteins c1, c2, c3 and c4 in the complementary sense. the c4 protein of monopartite begomoviruses has been implicated to play a role in symptom determination and virus movement. the present work aims at the biochemical characterization of this protein. | 2013 | 23500017 |
| functional characterization of coat protein and v2 involved in cell to cell movement of cotton leaf curl kokhran virus-dabawali. | the functional attributes of coat protein (cp) and v2 of the monopartite begomovirus, cotton leaf curl kokhran virus- dabawali were analyzed in vitro and in vivo by their overexpression in e. coli, insect cells and transient expression in the plant system. purified recombinant v2 and cp proteins were shown to interact with each other using elisa and surface plasmon resonance. confocal microscopy of sf21 cells expressing v2 and cp proteins revealed that v2 localized to the cell periphery and cp t ... | 2011 | 22110597 |
| kinetics of interaction of cotton leaf curl kokhran virus-dabawali (clcukv-dab) coat protein and its mutants with ssdna. | gemini viral assembly and transport of viral dna into nucleus for replication, essentially involve dna-coat protein interactions. the kinetics of interaction of cotton leaf curl kokhran virus-dabawali recombinant coat protein (rcp) with dna was studied by electrophoretic mobility shift assay (emsa) and surface plasmon resonance (spr). the rcp interacted with ssdna with a k(a), of 2.6+/-0.29 x 10(8) m(-1) in a sequence non-specific manner. the cp has a conserved c2h2 type zinc finger motif compos ... | 2009 | 19237182 |
| genetic variability of begomoviruses associated with cotton leaf curl disease originating from india. | cotton leaf curl disease (clcud) causing viruses belong to the begomovirus genus of the family geminiviridae. most begomoviruses are bipartite with two molecules of circular single stranded dna (a and b) encapsidated in icosahedral geminate particles. however, the begomoviruses associated with clcud have dna-beta instead of dna-b. in this communication we report the complete genomic sequence of dna-a component of two clcud-causing begomoviruses, cotton leaf curl kokhran virus-dabawali (clcukv-da ... | 2004 | 15669112 |