| rapid diagnosis of turbot reddish body iridovirus in turbot using the loop-mediated isothermal amplification method. | turbot reddish body iridovirus (trbiv) is a new piscine iridovirus that infects the turbot, scophthalmus maximus, cultured in northern china and can cause high mortality. in this study, a loop-mediated isothermal amplification (lamp) method was developed for the specific detection of this virus using primers designed from an msp i restriction dna fragment of the trbiv genome. mg(2+) concentrations, the reaction temperature, and the reaction time of lamp were optimized to 6mm, 65 degrees c, and 6 ... | 2009 | 19187785 |
| a novel heart-cell line from brown-marbled grouper epinephelus fuscoguttatus and its susceptibility to iridovirus. | a novel cell line (bmgh) was established from the heart of brown-marbled grouper epinephelus fuscoguttatus and its viral susceptibility was evaluated. the bmgh cells have been subcultured to passage 65 in dulbecco's modified eagle medium:ham's nutrient mixture f-12 (1:1) medium (dmem/f12) which was further supplemented with foetal bovine serum (fbs), carboxymethyl-chitosan, basic fibroblast growth factor (bfgf) and insulin-like growth factor-i (igf-i) at 24 degrees c. the heart cells have a fibr ... | 2010 | 20409167 |
| development and characterization of a new marine fish cell line from turbot (scophthalmus maximus). | a new marine fish cell line, tk, derived from turbot (scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. the tk cells were maintained in minimum essential medium eagle (mem) supplemented with hepes, antibiotics, fetal bovine serum (fbs), 2-mercaptoethanol (2-me), and basic fibroblast growth factor (bfgf). the suitable growth temperature for tk cells was 24°c, and microscopically, tk cells were comp ... | 2010 | 20496112 |
| establishment of a turbot fin cell line and its susceptibility to turbot reddish body iridovirus. | a turbot, scophthalmus maximus, fin (tf) cell line was established and susceptibility to turbot reddish body iridovirus (trbiv) was determined in this study. primary culture of tf cells was initiated from fin tissue pieces partially digested with trypsin, collagenase ii and hyaluronidase. digested tissue pieces were cultured at 24 degrees c in leibovitz-15 medium (ph 7.2), supplemented with 20% fetal bovine serum, carboxymethyl chitosan, n-acetylglucosamine hydrochloride, basic fibroblast growth ... | 2010 | 20502962 |
| complete genome sequence of a megalocytivirus (family iridoviridae) associated with turbot mortality in china. | turbot reddish body iridovirus (trbiv) causes serious systemic diseases with high mortality in the cultured turbot, scophthalmus maximus. we here sequenced and analyzed the complete genome of trbiv, which was identified in shandong province, china. | 2010 | 20630106 |
| development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of ostreid herpesvirus 1 dna. | a loop-mediated isothermal amplification (lamp) assay was developed for rapid, specific and sensitive detection of ostreid herpesvirus 1 (oshv-1) dna. a set of four primers was designed, based on the sequence of the atpase subunit of the oshv-1 dna-packaging terminase gene. the reaction temperature and time were optimized to 64°c and 60min, respectively. lamp products were detected by agarose gel electrophoresis or by visual inspection of a color change due to addition of fluorescent dye. the de ... | 2010 | 20813133 |
| cloning and expression analysis of interferon regulatory factor 7 (irf-7) in turbot, scophthalmus maximus. | interferon regulatory factor (irf) 7 is known as the master regulator of type i interferon (ifn)-dependent immune responses in mammals. in this study, the cdna and genomic sequences of turbot (scophthalmus maximus) irf-7 (smirf-7) were cloned and found to encode a putative protein of 439 amino acids. the gene is composed of 10 exons and 9 introns similar to known irf-7 genes of fish. the smirf-7 shows the highest amino acid identity of 49.0-80.3% to fish irf-7 and possesses a dna-binding domain ... | 2010 | 21147158 |
| establishment and characterization of a testicular cell line from the half-smooth tongue sole, cynoglossus semilaevis. | spermatogenesis within the adult testis is an excellent system for studying stem cell renewal and differentiation, which is under the control of testicular somatic cells. in order to understanding spermatogenesis in the half-smooth tongue sole (cynoglossus semilaevis) as a marine fish model of aquaculture importance, we established a cell line called csgc from a juvenile gonad of this organism. csgc is composed of fibroblast-like cells, retains a diploid karyotype of 42 chromosomes, lacks the he ... | 2011 | 21547062 |
| an irf-3 homolog that is up-regulated by dna virus and poly i:c in turbot, scophthalmus maximus. | in this study, we described the structure, mrna tissue distribution and regulation of an irf-3 gene from turbot, scophthalmus maximus (smirf-3). the gene sequence of smirf-3 is 6077 bp long, composed of 11 exons and 10 introns similar to known irf-3 genes of fish, and encodes a peptide of 466 amino acids. the deduced protein sequence shares the highest identity of 56.0-81.2% with fish irf-3 and possesses a dna-binding domain (dbd), an irf association domain (iad) and a serine-rich domain (srd) k ... | 2011 | 21782955 |
| Molecular characterization and expression analysis of interferon regulatory factor 5 (IRF-5) in turbot, Scophthalmus maximus. | Interferon regulatory factor 5 (IRF-5) plays a role both in the antiviral and inflammatory responses. In this study, we described the structure, mRNA tissue distribution and regulation of an IRF-5 gene from turbot, Scophthalmus maximus (SmIRF-5). The gene sequence of SmIRF-5 is 4275 bp long, composed of 9 exons and 8 introns similar to known IRF-5 genes of vertebrates, and encodes a peptide of 487 amino acids. The deduced protein sequence shares the highest identity of ~60-70% with fish IRF-5 an ... | 2012 | 22062122 |
| megalocytiviruses. | the genus megalocytivirus, represented by red sea bream iridovirus (rsiv), the first identified and one of the best characterized megalocytiviruses, infectious spleen and kidney necrosis virus (isknv), the type species of the genus, and numerous other isolates, is the newest genus within the family iridoviridae. viruses within this genus are causative agents of severe disease accompanied by high mortality in multiple species of marine and freshwater fish. to date outbreaks of megalocytivirus-ind ... | 2012 | 22590684 |
| detection of infectious spleen and kidney necrosis virus (isknv) and turbot reddish body iridovirus (trbiv) from archival ornamental fish samples. | although infections caused by megalocytiviruses have been reported from a wide range of finfish species for several decades, molecular characterisation of the viruses involved has been undertaken only on more recent cases. sequence analysis of the major capsid protein and adenosine triphosphatase genes is reported here from formalin-fixed, paraffin-embedded material from 2 archival ornamental fish cases from 1986 and 1988 in conjunction with data for a range of genes from fresh frozen tissues fr ... | 2016 | 28000602 |
| detection and molecular characterization of infectious spleen and kidney necrosis virus from major ornamental fish breeding states in peninsular malaysia. | 'gold standard' oie reference pcr assay was utilized to detect the presence of infectious spleen and kidney necrosis virus (isknv) in freshwater ornamental fish from malaysia. from total of 210 ornamental fish samples representing 14 species, isknv was detected in 36 samples representing 5 fish species. all positive cases did not show any clinical signs of isknv. three restriction enzymes analyses showed that the fish were infected by identical strains of the same virus species within megalocyti ... | 2014 | 23952914 |
| cloning and expression analysis of a toll-like receptor 21 (tlr21) gene from turbot, scophthalmus maximus. | toll-like receptor 21 (tlr21) is a non-mammalian tlr recognizing unmethylated cpg dna and considered as a functional homolog of mammalian tlr9. in the present study, a tlr21 gene was cloned from turbot, scophthalmus maximus, its immune responsive expression was subsequently studied in vivo. the turbot (sm)tlr21 gene is an intronless gene with a length of 3527 bp and encodes a peptide of 984 amino acids. the deduced protein possesses a signal peptide sequence, a leucine-rich repeat (lrr) domain c ... | 2017 | 28359672 |
| molecular cloning and expression analysis of interferon stimulated gene 15 (isg15) in turbot, scophthalmus maximus. | the interferon stimulated gene 15 (isg15) is strongly induced in many cell types by double-stranded rna (polyinosinic: polycytidylic acid, poly i:c) and viral infection. in this study, we described the nucleotide, mrna tissue distribution and regulation of an isg15 gene from turbot, scophthalmus maximus (smisg15). smisg15 gene is 862 bp in length, composed of two exons and one intron, and encodes 158 amino acids. the deduced protein exhibits the highest homology (44.7-71.2% identity) with isg15s ... | 2015 | 26095010 |
| molecular cloning and expression studies of the adapter molecule myeloid differentiation factor 88 (myd88) in turbot (scophthalmus maximus). | myeloid differentiation factor 88 (myd88) is an adapter protein involved in the interleukin-1 receptor (il-1r) and toll-like receptor (tlr)-mediated activation of nuclear factor-kappab (nf-κb). in this study, a full length cdna of myd88 was cloned from turbot, scophthalmus maximus. it is 1619 bp in length and contains an 858-bp open reading frame that encodes a peptide of 285 amino acid residues. the putative turbot (sm)myd88 protein possesses a n-terminal death domain and a c-terminal toll/il-1 ... | 2015 | 26025195 |
| cloning and expression analysis of a toll-like receptor 22 (tlr22) gene from turbot, scophthalmus maximus. | toll-like receptor 22 (tlr22) exists exclusively in aquatic animals and recognizes double stranded rna (dsrna). in the present study, a tlr22 gene and its 5'-flanking sequence were cloned from turbot, scophthalmus maximus, its immune responsive expression was subsequently studied in vivo. the turbot (sm)tlr22 gene spans over 5.6 kb with a structure of 4 exon-3 intron and encodes 962 amino acids. the deduced protein shows the highest sequence identity (76.7%) to japanese flounder tlr22 and posses ... | 2015 | 25770871 |
| a toll-like receptor 3 homologue that is up-regulated by poly i:c and dna virus in turbot scophthalmus maximus. | in this study, the gene and promoter sequences of turbot scophthalmus maximus (sm) toll-like receptor 3 (tlr3) were cloned and its mrna tissue distribution and gene expression in response to polyinosinic:polycytidylic acid (poly i:c) and turbot reddish body iridovirus (trbiv) challenges were studied in vivo. the smtlr3 gene spans over 4·4 kb with a structure of five exons-four introns and encodes a peptide of 916 amino acids. the putative protein shares the highest sequence identity of 52·8-78·5 ... | 2015 | 25631305 |
| cloning and expression study of an irf4a gene and its two transcript variants in turbot, scophthalmus maximus. | interferon regulatory factor 4 (irf4) is known to be involved in antiviral response as well as regulation of functional and developmental processes in lymphomyeloid cell lineages in mammals. in this study, the gene of irf4a and its two transcript variants (named irf4a1 and -2) were cloned from turbot, scophthalmus maximus, the tissue distributions and in vivo immune responsive expression patterns of the two transcripts were subsequently examined. the scophthalmus maximus (sm)irf4a gene is 8367 n ... | 2018 | 29054828 |