| mitochondrial genome of babesia orientalis, apicomplexan parasite of water buffalo (bubalus babalis, linnaeus, 1758) endemic in china. | apicomplexan parasites of the genus babesia, theileria and plasmodium are very closely related organisms. interestingly, their mitochondrial (mt) genomes are highly divergent. among babesia, babesia orientalis is a new species recently identified and specifically epidemic to the southern part of china, causing severe disease to water buffalo. however, no information on the mt genome of b. orientalis was available. | 2014 | 24580772 |
| molecular cloning and characterization of a novel heat shock protein 20 of babesia orientalis. | the heat shock protein 20 (hsp20) gene of babesia orientalis (bohsp20) was identified from both genomic dna and cdna. the full-length bohsp20 gene was 690bp with one intron from position 88-243bp. the amplicon obtained from cdna corresponded to a full-length open reading frame (orf) with a length of 534bp, encoding a polypeptide of 178 amino acid residues with a predicted size of 20kda. the orf was cloned into a pet-28a plasmid and subsequently expressed as a his-fusion protein. the recombinant ... | 2014 | 24857770 |
| molecular cloning and characterization of babesia orientalis rhoptry-associated protein 1. | the rhoptry-associated protein 1 (rap-1) gene of babesia orientalis was obtained from a cdna expression library by immunoscreening with b. orientalis-infected water buffalo sera. the nucleotide sequence of the cdna was 1732 bp with an open reading frame (orf) of 1434 bp, encoding a polypeptide of 478 amino acid residues with a predicted size of 52.5 kda. the orf was cloned into a pgex-kg plasmid and subsequently expressed as a gst-fusion protein. the recombinant rap-1 of b. orientalis (rborap-1) ... | 2014 | 25199690 |
| identification and characterization of a novel 34 kda merozoite protein in babesia orientalis. | a novel babesia orientalis 34 kda protein (designated bop34) was obtained by immunoscreening of a cdna expression library using b. orientalis infected water buffalo serum. the complete nucleotide sequence of the bop34 was 1088 bp, which contained one open reading frame (orf), two untranslated regions (utrs) and a poly (a) tail. the length of orf was 933 bp, encoding a polypeptide of 310 aa with a predicted size of 34 kda. blast analysis showed that the nucleotide sequence of bop34 had 71% simila ... | 2015 | 26169218 |
| characterization and annotation of babesia orientalis apicoplast genome. | babesia orientalis is an obligate intraerythrocytic protozoan parasite of the buffalo (bubalus bubalis, linnaeus, 1758) transmitted by the tick rhipicephalus heamaphysaloides. it is the causative agent of water buffalo babesiosis, one of the most important pathogens of water buffalo in central and southern china. as a member of the phylum apicomplexa, b. orientalis possesses a relatively independent and alga originated organelle the apicoplast. apicoplasts in other apicomplexa parasites are invo ... | 2015 | 26474853 |
| observation of a novel babesia spp. in eastern grey kangaroos (macropus giganteus) in australia. | the roles and epidemiological features of tick-borne protozoans are not well elicited in wildlife. babesia spp. are documented in many domestic animals, including cattle, horses, pigs, dogs and cats. three cases affecting eastern grey kangaroos are described. the kangaroos exhibited neurological signs, depression and marked anaemia, and microscopic examination of blood smears revealed intraerythrocytic piroplasms. one to seven intraerythrocytic spherical, oval, pyriform and irregularly-shaped pa ... | 2012 | 24533316 |
| identification of two novel hsp90 proteins in babesia orientalis: molecular characterization, and computational analyses of their structure, function, antigenicity and inhibitor interaction. | hsp90 protects the cells from heat stress and facilitates protein maturation and stability. the full genome sequences of piroplasms contain two putative hsp90 proteins, which are yet uncharacterized. to this end, the two putative hsp90 proteins of babesia orientalis were identified and characterized by molecular and in silico methods. | 2014 | 24970594 |
| occurrence of theileria and babesia species in water buffalo (bubalus babalis, linnaeus, 1758) in the hubei province, south china. | the presence and prevalence of tick-borne haemoparasites in water buffalo from the hubei province, south china was investigated using the reverse line blot (rlb) hybridization assay and phylogenetic analysis of the parasite 18s rrna gene. theileria buffeli (19.1%) was the most frequently found species in all of the locations, followed by babesia orientalis (8.9%), babesia bovis (1.0%) and babesia bigemina (0.7%). only 12 (3.9%) of the samples had mixed infections. eleven samples with single infe ... | 2011 | 22154255 |
| development and evaluation of real-time pcr assay for the detection of babesia orientalis in water buffalo (bubalus bubalis, linnaeus, 1758). | abstract babesia orientalis is the causative agent of babesiosis in water buffalo (bubalus babalis, linnaeus, 1758). in this study, a taqman real-time pcr assay was developed for quantitative detection of b. orientalis in water buffalo. hybridization probe and oligonucleotide primers were designed based on the v4 region of 18s rrna gene. detection limit was determined at 2 parasites. blood samples were collected from experimentally infected water buffalo, as well as from 180 field samples, which ... | 2011 | 21711103 |
| an indirect elisa for detection of theileria sergenti antibodies in water buffalo using a recombinant major piroplasm surface protein. | in this study we investigated the prevalence and enzootic potential of theileria spp. in water buffalo in the hubei province in china. an indirect elisa based on a recombinant major piroplasma surface protein was developed. the complete orf of the 33-kda major piroplasma surface protein (p33) was obtained from theileria sergenti genomic dna by pcr, cloned into the pet-28(a) vector and expressed in e. coli as a his-fusion protein. then the recombinant p33 (rp33) was purified and used as the antig ... | 2010 | 20207484 |
| [construction and immunoscreening of cdna library of babesia orientalis]. | to construct a cdna library for babesia orientalis and screen immunologically positive clones. | 2009 | 19852360 |
| loop-mediated isothermal amplification (lamp) detection of babesia orientalis in water buffalo (bubalus babalis, linnaeus, 1758) in china. | loop-mediated isothermal amplification (lamp) is a rapid method with high specificity and efficiency under isothermal condition using a set of four specifically designed primers that recognize six distinct sequences on the target gene. in this study, a lamp method was developed for specific detection of babesia orientalis in water buffalo (bubalus babalis, linnaeus, 1758). four primers were designed from the v4 hypervariable region of the 18s rrna gene of b. orientalis. blood samples were collec ... | 2009 | 19665847 |
| molecular cloning and phylogenetic analysis of babesia orientalis heat shock protein 70. | the heat shock protein 70 (hsp70) gene of babesia orientalis was obtained from a cdna expression library by immunoscreening with b. orientalis infected buffalo sera. the nucleotide sequence of the cdna was 2192bp with an open reading frame (orf) of 1944bp encoding a polypeptide of 648 amino acid residues. phylogenetic analysis of the 1944bp sequence together with 30 inter-erythrocytic protozoa hsp70 nucleotide sequences available from genbank was performed. the results showed that b. orientalis ... | 2009 | 19375234 |
| a 29-kda merozoite protein is a prospective antigen for serological diagnosis of babesia orientalis infection in buffaloes. | a gene encoding a 29-kda protein of babesia orientalis (bop29) was isolated by immunoscreening the b. orientalis cdna expression library using monoclonal antibodies and its nucleotide sequence of the cdna was 971bp with an open reading frame of 750bp. the results of blastn and blastx in ncbi showed that this cdna sequence had similarity with babesia bovis hypothetical protein (bbov_ii005480). western blotting showed that the recombinant protein (rbop29) had positive seroreactivity to b. oriental ... | 2009 | 19345505 |
| semi-nested pcr detection of babesia orientalis in its natural hosts rhipicephalus haemaphysaloides and buffalo. | babesiosis has recently been recognized as an emerging infectious disease of buffalo in china. in order to investigate the epidemiology and enzootic potential of this parasite in hubei province, we sought to develop a semi-nested pcr to detect babesia orientalis in buffalo and the potential tick vector-rhipicephalus haemaphysaloides by amplifying a specific 257bp fragment of b. orientalis 18s rrna gene. the practical limit of detection showed that it had high sensitivity and an approximate paras ... | 2007 | 17005323 |
| study on some molecular characterization of babesia orientalis. | the study on buffalo babesiosis indicated that its pathogen was different from other babesia on many aspects such as morphology, transmission and pathogenicity. therefore, it was named as a new species-babesia orientalis. in order to prove the validity of this taxon, molecular taxonomic study on the pathogen was done in this experiment. the complete 18s rrna gene sequence of b. orientalis was determined by pcr. it was sequenced and blasted. the results indicated that the classification of the pa ... | 2005 | 15925722 |
| serological investigations on babesia orientalis infection. status of water buffaloes in hubei province. | a total of 230 water buffaloes were sampled for the determination of the epidemiological situation of babesia orientalis infection in hubei province, using the latex agglutination test. this serological test revealed that 74.7% of the collected sera were positive for antibodies against b. orientalis in endemic areas. in contrast, only 1.79% of water buffaloes were positive in non-epidemic areas. the incidence of the infection was substantially higher in older than in younger animals. the results ... | 2002 | 12051598 |
| culture-derived babesia orientalis exoantigens used as a vaccine against buffalo babesiosis. | buffalo babesiosis represents a major problem for the livestock industry in china. the pathogen of this disease was isolated and identified as babesia orientalis and subsequently propagated in vitro, using the microaerophilus stationary phase culture system. the aim of this study was to determine the efficacy of exoantigens derived from b. orientalis cultures to induce a protective immunity against challenge exposure to virulent organisms in laboratory and field. the results showed that exoantig ... | 2002 | 12051606 |
| a historical overview of research on babesia orientalis, a protozoan parasite infecting water buffalo. | babesiosis is a globally important zoonotic disease caused by tick-borne intraerythrocytic protozoan of the genus babesia (phylum apicomplexa). in china, there are five species that infect cattle buffalo and cause great economic loss, which include babesia bigemina, b. bovis, b. major, b. ovata, and b. orientalis. among them, b. orientalis is the most recently identified new babesia species epidemic in china. this review summarized the work done in the past 33 years to give an overview of what l ... | 2017 | 28769894 |
| molecular cloning and characterization of babesia orientalis rhoptry neck 2 boron2 protein. | babesiosis caused by babesia orientalis is one of the most prevalent infections of water buffalo transmitted by rhipicephalus haemaphysaloides causing a parasitic and hemolytic disease. the organelles proteins localized in apical membrane especially rhoptries neck and microneme protein form a complex called moving junction important during invasion process of parasites belonging to apicomplexan group, including babesia species. a truncated fragment coding a 936 bps fragment was cloned in pmd-19t ... | 2017 | 28775897 |
| characterisation of a babesia orientalis apical membrane antigen, and comparison of its orthologues among selected apicomplexans. | in the present study, we identified and characterised the complete coding sequence of babesia orientalis apical membrane antigen 1 (designated bo-ama1); it is 1803bp in length and encodes a polypeptide of 601 amino acids (aa). the bo-ama-1 gene product (bo-ama1) is predicted to be 67kda in size and contains a signal peptide. mature bo-ama1 is predicted to have one transmembrane region and a short cytoplasmic tail (c-terminal domain). the extracellular part of bo-ama1 has three functional domains ... | 2015 | 25732411 |
| a survey of canine haemoprotozoan parasites from turkey, including molecular evidence of an unnamed babesia. | canine tick-borne apicomplexan parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. a reverse line blot assay was performed on 219 blood samples collected from domestic dogs for simultaneous detection of all named canine piroplasm species as well as hepatozoon canis. ten samples hybridized to the theileria/babesia and babesia catch all probes but did not hybridize to any species-specific probe tested, suggesting the presence of an unrec ... | 2017 | 28673460 |