| strain characterization of candida parapsilosis fungemia by molecular typing methods. | the present study used two molecular typing methods to investigate a cluster of eight cases of candida parapsilosis fungemia in a hospital in rio de janeiro, brazil. candida parapsilosis is an important opportunistic pathogen that is frequently involved in outbreaks of nosocomial fungemia. identification of a common source of infection and determination of genetic relatedness among the strains involved in outbreaks are important for infection control. candida parapsilosis strains were isolated f ... | 2000 | 10968322 |
| candida orthopsilosis and candida metapsilosis spp. nov. to replace candida parapsilosis groups ii and iii. | two new species, candida orthopsilosis and c. metapsilosis, are proposed to replace the existing designations of c. parapsilosis groups ii and iii, respectively. the species c. parapsilosis is retained for group i isolates. attempts to construct a multilocus sequence typing scheme to differentiate individual strains of c. parapsilosis instead revealed fixed dna sequence differences between pairs of subgroups in four genes: cox3, l1a1, sadh, and sya1. pcr amplicons for sequencing were obtained fo ... | 2005 | 15634984 |
| rapid identification of fungal pathogens in bact/alert, bactec, and bbl mgit media using polymerase chain reaction and dna sequencing of the internal transcribed spacer regions. | we report a direct polymerase chain reaction/sequence (d-pcrs)-based method for the rapid identification of clinically significant fungi from 5 different types of commercial broth enrichment media inoculated with clinical specimens. media including bact/alert fa (biomérieux, marcy l'etoile, france) (n = 87), bactec plus aerobic/f (becton dickinson, microbiology systems, sparks, md) (n = 16), bactec peds plus/f (becton dickinson) (n = 15), bactec lytic/10 anaerobic/f (becton dickinson) (n = 11) b ... | 2006 | 16466900 |
| complete dna sequences of the mitochondrial genomes of the pathogenic yeasts candida orthopsilosis and candida metapsilosis: insight into the evolution of linear dna genomes from mitochondrial telomere mutants. | we determined complete mitochondrial dna sequences of the two yeast species, candida orthopsilosis and candida metapsilosis, and compared them with the linear mitochondrial genome of their close relative, c.parapsilosis. mitochondria of all the three species harbor compact genomes encoding the same set of genes arranged in the identical order. differences in the length of these genomes result mainly from the presence/absence of introns. multiple alterations were identified also in the sequences ... | 2006 | 16684995 |
| candida hyderabadensis sp. nov., a novel ascomycetous yeast isolated from wine grapes. | three ascomycetous yeast strains were isolated from decaying green wine grapes, collected from hyderabad city in india. two strains, ys9 and ys21, were identified as kodamaea ohmeri and candida fermentati, respectively. the third strain, ys12(t), differs from candida parapsilosis, candida orthopsilosis and candida metapsilosis, the nearest phylogenetic neighbours, by 1.6-1.9% with respect to the nucleotide sequence of the d1/d2 domain of the 26s rrna gene and by 1.4-9.2% with respect to the nucl ... | 2007 | 17253980 |
| genotyping of candida orthopsilosis clinical isolates by amplification fragment length polymorphism reveals genetic diversity among independent isolates and strain maintenance within patients. | candida parapsilosis former groups ii and iii have recently been established as independent species named c. orthopsilosis and c. metapsilosis, respectively. in this report, 400 isolates (290 patients) previously classified as c. parapsilosis by conventional laboratory tests were screened by bani digestion profile analysis of the secondary alcohol dehydrogenase gene fragment and by amplification fragment length polymorphism (aflp). thirty-three strains collected from 13 patients were identified ... | 2007 | 17329454 |
| virulence of candida parapsilosis, candida orthopsilosis, and candida metapsilosis in reconstituted human tissue models. | candida parapsilosis is an increasingly important human pathogen. to study the interactions of c. parapsilosis with human tissues, we evaluated the effects of the cbs 604 type strain and three different clinical isolates on reconstituted human oral epithelial and epidermal tissues. the newly described species candida orthopsilosis and candida metapsilosis were also examined in these models. microscopy of reconstituted tissues infected with yeast cells revealed severe attenuation, morphological c ... | 2007 | 17391997 |
| yeasts isolated from plant-associated beetles and other insects: seven novel candida species near candida albicans. | yeasts related to candida albicans were isolated from the digestive tracts of beetles in eight families and various orders of insects such as earwigs, crickets, and roaches, most of which were caught at light traps or in a few cases directly from plant materials. based on comparisons of dna sequences and other taxonomic characteristics, a total of 41 isolates were identified as candida orthopsilosis, candida pseudorhagii, candida maltosa, candida parapsilosis, candida tropicalis, candida neerlan ... | 2008 | 17986254 |
| dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. | an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ... | 2008 | 18160452 |
| molecular identification of candida orthopsilosis isolated from blood culture. | the incidence of candidemia and invasive candidiasis have increased markedly due to the increasing number of immunocompromised patients. there are five major medically important species of candida with their frequency of isolation in the diminishing order namely candida albicans, candida parapsilosis, candida tropicalis, candida glabrata and candida krusei. in addition, there are numerous other species of candida which differ in their genetic makeup, virulence properties, drug susceptibilities a ... | 2008 | 18266075 |
| prevalence and susceptibility profile of candida metapsilosis and candida orthopsilosis: results from population-based surveillance of candidemia in spain. | we describe the prevalences and susceptibility profiles of two recently described species, candida metapsilosis and candida orthopsilosis, related to candida parapsilosis in candidemia. the prevalences of these species (1.7% for c. metapsilosis and 1.4% for c. orthopsilosis) are significant. differences observed in their susceptibility profiles could have therapeutic importance. | 2008 | 18285486 |
| a naturally occurring proline-to-alanine amino acid change in fks1p in candida parapsilosis, candida orthopsilosis, and candida metapsilosis accounts for reduced echinocandin susceptibility. | candida parapsilosis has emerged as a common cause of invasive fungal infection, especially in latin america and in the neonatal setting. c. parapsilosis is part of a closely related group of organisms that includes the species candida orthopsilosis and candida metapsilosis. all three species show elevated mics for the new echinocandin class drugs caspofungin, micafungin, and anidulafungin relative to other candida species. despite potential impacts on therapy, the mechanism behind this reduced ... | 2008 | 18443110 |
| significant differences in drug susceptibility among species in the candida parapsilosis group. | candida parapsilosis family has 3 proposed species: c. parapsilosis sensu stricto, candida orthopsilosis, and candida metapsilosis. c. parapsilosis sensu stricto had significantly higher caspofungin (cas) and anidulafungin mics than c. orthopsilosis or c. metapsilosis; c. metapsilosis was least susceptible to fluconazole. c. parapsilosis sensu stricto more frequently displayed (37%) paradoxical growth in cas (p < or = 0.02). these species susceptibility differences could affect therapeutic choic ... | 2008 | 18555634 |
| geographic distribution and antifungal susceptibility of the newly described species candida orthopsilosis and candida metapsilosis in comparison to the closely related species candida parapsilosis. | candida orthopsilosis and candida metapsilosis are recently described species, having previously been grouped with the more prevalent species candida parapsilosis. current literature contains very little data pertaining to the distributions and antifungal susceptibilities of these candida species. we determined the species and antifungal susceptibilities of 1,929 invasive clinical isolates from the artemis antifungal surveillance program collected between 2001 and 2006 and identified as c. parap ... | 2008 | 18562582 |
| comparison of killing activity of caspofungin against candida parapsilosis, candida orthopsilosis and candida metapsilosis. | | 2008 | 18824459 |
| molecular differentiation and antifungal susceptibilities of candida parapsilosis isolated from patients with bloodstream infections. | the genetic heterogeneity and antifungal susceptibility patterns of candida parapsilosis isolated from blood cultures of patients were investigated in this study. randomly amplified polymorphic dna (rapd) analysis generated 5 unique profiles from 42 isolates. based on the major dna fragments of the rapd profiles, the isolates were identified as rapd type p1 (29 isolates), p2 (6 isolates), p3 (4 isolates), p4 (2 isolates) and p5 (1 isolate). sequence analysis of the internal transcribed spacer (i ... | 2009 | 19141735 |
| correlation of restriction fragment length polymorphism genotyping with internal transcribed spacer sequence, randomly amplified polymorphic dna and multilocus sequence groupings for candida parapsilosis. | epidemiological studies of candida parapsilosis have been performed by molecular methods. to compare two prominent methods, 29 isolates, typed by multilocus sequence typing (mlst), were typed by restriction fragment length polymorphism (rflp). of the 19 proposed candida parapsilosis sensu stricto isolates [group i by internally transcribed spacer (its1) sequence], the most commonly encountered species, 17 were rflp type vii-1. the species candida orthopsilosis (eight isolates) and candida metaps ... | 2009 | 19207835 |
| in vitro efficacy of 5 antifungal agents against candida parapsilosis, candida orthopsilosis, and candida metapsilosis as determined by time-kill methodology. | killing activity of amphotericin b, fluconazole, voriconazole, posaconazole, and 5-fluorocytosine was determined against 6 candida parapsilosis, 3 candida orthopsilosis, and 4 candida metapsilosis clinical isolates. after 24 h, 1 of 6 c. parapsilosis, 1 of 3 c. orthopsilosis, and 3 of 4 c. metapsilosis isolates were killed at 1 to 4 microg/ml (1-8x mic) amphotericin b. the remaining isolates were killed by 2 to 4 microg/ml amphotericin b after 48 h. fluconazole was fungistatic at > or =1x mic (0 ... | 2009 | 19394787 |
| pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates candida parapsilosis, candida metapsilosis, and candida orthopsilosis. | two new cryptic sister species, candida orthopsilosis and candida metapsilosis, were recently identified by consistent dna sequence differences among several genes within the genetically heterogeneous candida parapsilosis complex. here, we present data demonstrating that pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related candida species. | 2009 | 19403763 |
| rapid molecular differentiation and genotypic heterogeneity among candida parapsilosis and candida orthopsilosis strains isolated from clinical specimens in kuwait. | recent molecular studies have led to the recognition of three distinct species within the candida parapsilosis complex, namely candida parapsilosis, candida orthopsilosis and candida metapsilosis. as currently available yeast identification systems fail to differentiate these species, there is a paucity of information on their occurrence in different geographical regions. this study describes a simple pcr-based protocol for rapid discrimination among c. parapsilosis, c. orthopsilosis and c. meta ... | 2009 | 19429750 |
| yeast diversity of ghanaian cocoa bean heap fermentations. | the fermentation of the theobroma cacao beans, involving yeasts, lactic acid bacteria, and acetic acid bacteria, has a major influence on the quality of the resulting cocoa. an assessment of the microbial community of cocoa bean heap fermentations in ghana resulted in 91 yeast isolates. these were grouped by pcr-fingerprinting with the primer m13. representative isolates were identified using the d1/d2 region of the large subunit rrna gene, internal transcribed spacer sequences and partial actin ... | 2009 | 19473277 |
| prevalence rates and antifungal susceptibility profiles of the candida parapsilosis species complex: results from a nationwide surveillance of candidaemia in brazil. | the genetically heterogeneous taxon candida parapsilosis was recently reclassified into three species: candida parapsilosis, candida orthopsilosis and candida metapsilosis. the prevalences of these species among 141 bloodstream isolates tested in brazil were 88% for c. parapsilosis, 9% for c. orthopsilosis, and 3% for c. metapsilosis. except for three c. orthopsilosis isolates that were considered resistant to 5-flucytosine, all isolates representing the different species of this complex were su ... | 2010 | 19686280 |
| molecular screening for candida orthopsilosis and candida metapsilosis among danish candida parapsilosis group blood culture isolates: proposal of a new rflp profile for differentiation. | candida orthopsilosis and candida metapsilosis are recently described species phenotypically indistinguishable from candida parapsilosis . we evaluated phenotyping and molecular methods for the detection of these species among 79 unique blood culture isolates of the c. parapsilosis group obtained during the years 2004-2008. the isolates were screened by pcr amplification of the secondary alcohol dehydrogenase-encoding gene ( sadh) followed by digestion with the restriction enzyme ban i, using c. ... | 2010 | 20056771 |
| candida metapsilosis as the least virulent member of the 'c. parapsilosis' complex. | results of recent molecular studies have provided evidence of three distinct species within the candida parapsilosis complex, namely candida parapsilosis, candida orthopsilosis and candida metapsilosis. while there are initial data pertaining to the virulence of these candida species with respect to reconstituted epidermal and oral epithelial tissues, there have been no studies, as of yet, on their interaction with immune cells. employing an in vitro infection model using microglial cells, we in ... | 2010 | 20507266 |
| characterization of candida parapsilosis complex isolates. | candida parapsilosis former groups ii and iii have recently been established as independent species, named candida orthopsilosis and candida metapsilosis, respectively. we investigated the distribution of c. parapsilosis complex species in 122 isolates from blood and other sources in a southern spain tertiary-care hospital, and we examined the relationship between species, site of isolation and biofilm positivity. we also evaluated the planktonic mics and sessile mics (smics) of voriconazole, am ... | 2011 | 20636431 |
| molecular epidemiology and antifungal susceptibility of candida parapsilosis sensu stricto, candida orthopsilosis, and candida metapsilosis in taiwan. | candida parapsilosis was recently reclassified into 3 closely related species, c. parapsilosis sensu stricto, candida orthopsilosis, and candida metapsilosis. variation in susceptibility characteristics and prevalence of the 3 genomic species could have therapeutic and epidemiologic implications. the aim of this study is to characterize the genetic and antifungal susceptibility profiles of 97 c. parapsilosis isolates from 71 patients. among the 71 nonduplicate isolates, 85.9% (61/71) were identi ... | 2010 | 20851551 |
| [emergence of candida parapsilosis candidemia at cochin hospital. characterization of isolates and search for risk factors]. | candida parapsilosis is a normal saprophyte of the skin, characterized by their affinity for catheters. this species has, in vitro, a level of sensitivity against the echinocandins, significantly lower than that observed with other candida species. recently, new species: candida orthopsilosis and candida metapsilosis, phenotypically identical to c. parapsilosis, have been identified by molecular biology. from 2003 to 2007, in the cochin hospital, the proportion of c. parapsilosis among non-albic ... | 2011 | 20889268 |
| improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. | clin microbiol infect abstract: the key to therapeutic success with yeast infections is an early onset of antifungal treatment with an appropriate drug regimen. to do this, yeast species identification is necessary, but conventional biochemical and morphological approaches are time-consuming. the recent arrival of biophysical methods, such as matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms), in routine diagnostic laboratories holds the promise of signif ... | 2010 | 20946411 |
| biofilm production and evaluation of antifungal susceptibility amongst clinical candida spp. isolates, including strains of the candida parapsilosis complex. | candida cells can form biofilms that frequently are sources of infections and are less susceptible to antifungal drugs. some authors have reported that candida orthopsilosis and candida metapsilosis isolates are not able to produce biofilms in vitro and there are no studies available on biofilm susceptibility for these species to antifungals. the aims of this study were to (i) quantify candida spp. biofilms in vitro, and (ii) test the in vitro susceptibilities of candida spp. biofilms to flucona ... | 2010 | 21039308 |
| rapid and accurate identification of species belonging to the candida parapsilosis complex by real-time pcr and melting curve analysis. | candida parapsilosis is the second most frequent candida species isolated from blood cultures. since 2005, c. parapsilosis has been divided into three distinct species based on genetic traits: candida parapsilosis, candida metapsilosis and candida orthopsilosis. the aim of this study was to develop a rapid real-time pcr assay able to distinguish these closely related species via a melting curve analysis. this identification method was optimized by using reference strains and well-characterized c ... | 2010 | 21183600 |
| evolution of mating within the candida parapsilosis species group. | candida orthopsilosis and candida metapsilosis are closely related to candida parapsilosis, a major cause of infection in premature neonates. mating has not been observed in these species. we show that ~190 isolates of c. parapsilosis contain only an mtla idiomorph at the mating-type-like locus. here, we describe the isolation and characterization of the mtl loci from c. orthopsilosis and c. metapsilosis. among 16 c. orthopsilosis isolates, 9 were homozygous for mtla, 5 were homozygous for mtla, ... | 2011 | 21335529 |
| characterization of oleaginous yeasts revealed two novel species: trichosporon cacaoliposimilis sp. nov. and trichosporon oleaginosus sp. nov. | two new species in the anamorphic basidiomycetous genus trichosporon (tremellomycetes, agaricomycotina) were uncovered in a dna sequence-based molecular analysis of oleaginous yeasts maintained in the atcc mycology collection. one yeast is named as trichosporon cacaoliposimilis sp. nov for its capability of synthesizing and accumulating a large amount of lipids having a composition equivalent to that of natural cacao butter. the type strain is atcc 20505t, originally deposited as trichosporon sp ... | 2011 | 21558504 |
| separation of phenotypically indistinguishable candida species, c. orthopsilosis, c. metapsilosis and c. parapsilosis, by capillary electromigration techniques. | at the current state of laboratory diagnostics, methods for fast identification of phenotypically indistinguishable species are difficult or inaccurate. an example is represented by candida parapsilosis, which is the second most common yeast species isolated from bloodstream infections. c. parapsilosis comprises a complex of three genetically distinct groups. genotypes ii and iii have been designated as the separate species candida orthopsilosis and candida metapsilosis, phenotypically indisting ... | 2011 | 21571287 |
| comparative in vitro susceptibility of clinical isolates of candida paparsilosis complex and other candida species to caspofungin and anidulafungin by etest. | in vitro susceptibility of 141 clinical isolates of candida species to caspofungin and anidulafungin is reported. the etest was performed according to recommended procedure and minimum inhibitory concentrations (mics) were read after 24 h of incubation at 35°c. applying a breakpoint of <2 mg/ml, all candida spp. isolates, except those belonging to c. parapsilosis complex, were susceptible. the geometric mean for caspofungin and anidulafungin for different candida spp. were as follows: candida pa ... | 2011 | 21571626 |
| prevalence and antifungal susceptibility patterns of new cryptic species inside the species complexes candida parapsilosis and candida glabrata among blood isolates from a spanish tertiary hospital. | objectives there is scarce information on the clinical relevance and antifungal susceptibility of candida bracarensis, candida nivariensis, candida orthopsilosis and candida metapsilosis. the objective of this study was to assess the prevalence and in vitro antifungal susceptibility of these cryptic species among 173 blood isolates previously identified as candida glabrata or candida parapsilosis at the hospital of cruces (barakaldo, spain). the survey was extended to 518 clinical isolates from ... | 2011 | 21795259 |
| anticandidal efficacy of cinnamon oil against planktonic and biofilm cultures of candida parapsilosis and candida orthopsilosis. | candida parapsilosis is yeast capable of forming biofilms on medical devices. novel approaches for the prevention and eradication of the biofilms are desired. this study investigated the anticandidal activity of sixteen essential oils on planktonic and biofilm cultures of c. parapsilosis complex. we used molecular tools, enumeration of colony-forming units, the colourimetric mtt assay, scanning electron microscopy (sem) and a chequerboard assay coupled with software analyses to evaluate the grow ... | 2011 | 21761153 |
| diversity of bacteria and yeast in the naturally fermented cotton seed and rice beverage produced by brazilian amerindians. | microorganisms associated with the fermentation of cotton seed and rice were studied using a combination of culture-dependent and -independent methods. samples of the cotton seed and rice beverage were collected every 8-áh during the fermentation process for analysis of the microbiota present over 48-áh. the lactic acid bacteria (lab) population reached values of approximately 8.0-álog-ácfu/ml. a total of 162 bacteria and 81 yeast isolates were identified using polyphasic methods. lab (lactobaci ... | 2011 | 21839389 |
| candida theae sp. nov., a new anamorphic beverage-associated member of the lodderomyces clade. | four strains representing a novel yeast species belonging to the genus candida were independently isolated in taiwan and ecuador. two strains (g17(t) and g31) were isolated in taiwan, by pellet precipitation from plastic-bottled tea drinks produced in indonesia, while two additional strains (clqca 10-049 and clqca 10-062) were recovered from ancient chicha fermentation vessels found in tombs in quito, ecuador. these four strains were morphologically, and phylogenetically identical to each other. ... | 2011 | 22088606 |
| Prospective multicenter study of the epidemiology, molecular identification, and antifungal susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis isolated from patients with candidemia. | A 13-month prospective multicenter study including 44 hospitals was carried out to evaluate the epidemiology of Candida parapsilosis complex candidemia in Spain. Susceptibility to amphotericin B, flucytosine, fluconazole, itraconazole, voriconazole, posaconazole, anidulafungin, caspofungin, and micafungin was tested by the microdilution colorimetric method. A total of 364 C. parapsilosis complex isolates were identified by molecular methods: C. parapsilosis (90.7%), Candida orthopsilosis (8.2%), ... | 2011 | 21930869 |
| Effect of Usnic Acid on Candida orthopsilosis and C. parapsilosis. | The activity of usnic acid against Candida orthopsilosis and Candida parapsilosis on planktonic and biofilm conditions was investigated by using a broth microdilution and microplate methods. Potent in vitro activities against different Candida species were obtained. The metabolic activity of sessile cells of C. parapsilosis complex was reduced by 80% at four times the 80% inhibitory concentration. The in vitro studies support further efforts to determine whether usnic acid can be used clinically ... | 2012 | 22006006 |
| Prevalence and Distribution Profiles of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis Responsible for Superficial Candidiasis in a Chinese University Hospital. | The Candida parapsilosis complex consists of C. parapsilosis sensu stricto, C. orthopsilosis and C. metapsilosis. Recently, many studies described the prevalence of this species complex mainly in invasive candidiasis. Additionally, data showed that these three species are different in virulence and in vitro drug susceptibility. However, to our knowledge, the prevalence and distribution of the species complex in superficial candidiasis is not very clear to date. In this study, 2,128 Candida isola ... | 2011 | 22102007 |
| Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals. | Candida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate th ... | 2011 | 22012217 |
| pharmacokinetic/pharmacodynamic analysis of voriconazole against candida spp. and aspergillus spp. in children, adolescents and adults by monte carlo simulation. | the objective of this study was to investigate the cumulative fraction of response of various voriconazole dosing regimens against six candida and six aspergillus spp. in immunocompromised children, immunocompromised adolescents, and adults. using pharmacokinetic parameters and pharmacodynamic data, 5000-subject monte carlo simulations (mcss) were conducted to evaluate the ability of simulated dosing strategies in terms of fauc/mic targets of voriconazole. according to the results of the mcss, c ... | 2016 | 27179818 |
| activity of a long-acting echinocandin (cd101) and seven comparator antifungal agents tested against a global collection of contemporary invasive fungal isolates in the sentry 2014 antifungal surveillance program. | the activity of cd101 and comparator antifungal agents against 606 invasive fungal isolates collected worldwide during 2014 was evaluated using the clinical and laboratory standards institute (clsi) method. all candida albicans (n = 251), candida tropicalis (n = 51), candida krusei (n = 16), and candida dubliniensis (n = 11) isolates were inhibited by ≤0.12 μg/ml of cd101 and were susceptible or showed wild-type susceptibility to the other echinocandins tested. five c. glabrata isolates (n = 100 ... | 2017 | 28052853 |
| candidaemia due to candida parapsilosis species complex at a hospital in brazil: clinical characteristics and antifungal susceptibility profile. | recent decades have seen a global emergence of candidaemia caused by non-candida albicans candida species, particularly the candida parapsilosis complex. | 2017 | 28214273 |
| epidemiological investigation of candida species causing bloodstream infection in paediatric small bowel transplant recipients. | small bowel transplantation (sbt) can be a life-saving medical procedure. however, these recipients experience high risk of bloodstream infections caused by candida. this research aims to characterise the sbt recipient gut microbiota over time following transplantation and investigate the epidemiology of candidaemia in seven paediatric patients. candida species from the recipients' ileum and bloodstream were identified by internal transcribed spacer sequence and distinguished to strain by multil ... | 2017 | 28139856 |
| identification of non-coding rnas in the candida parapsilosis species group. | the candida ctg clade is a monophyletic group of fungal species that translates ctg as serine, and includes the pathogens candida albicans and candida parapsilosis. research has typically focused on identifying protein-coding genes in these species. here, we use bioinformatic and experimental approaches to annotate known classes of non-coding rnas in three ctg-clade species, candida parapsilosis, candida orthopsilosis and lodderomyces elongisporus. we also update the annotation of ncrnas in the ... | 2017 | 27658249 |
| in vitro activity of anidulafungin in combination with amphotericin b or voriconazole against biofilms of five candida species. | to evaluate the in vitro activity of anidulafungin combined with amphotericin b or voriconazole against candida spp. biofilms. | 2016 | 27543657 |
| postantifungal effect of caspofungin against the candida albicans and candida parapsilosis clades. | killing and postantifungal effects could be relevant for the selection of optimal dosing schedules. this study aims to compare time-kill and postantifungal effects with caspofungin on candida albicans (c. albicans, candida dubliniensis, candida africana) and candida parapsilosis (c. parapsilosis, candida metapsilosis, candida orthopsilosis) clades. in the postantifungal effect experiments, strains were exposed to caspofungin for 1 h at concentrations 0.12-8 μg/ml. time-kill experiments were cond ... | 2016 | 27492134 |
| fungal peritonitis in patients undergoing peritoneal dialysis (pd) in brazil: molecular identification, biofilm production and antifungal susceptibility of the agents. | this paper presents data on fungal peritonitis (fp) in patients undergoing peritoneal dialysis (pd) at the university hospital of botucatu medical school, são paulo, brazil. in a total of 422 patients, 30 developed fp, from which the medical records and the fungal isolates of 23 patient cases were studied. all patients presented abdominal pain, cloudy peritoneal effluent, needed hospitalization, had the catheter removed and were treated with fluconazole or fluconazole plus 5-flucitosine; six of ... | 2016 | 27143636 |
| yeast microbiota of natural cavities of manatees (trichechus inunguis and trichechus manatus) in brazil and its relevance for animal health and management in captivity. | the aim of this study was to characterize the yeast microbiota of natural cavities of manatees kept in captivity in brazil. sterile swabs from the oral cavity, nostrils, genital opening, and rectum of 50 trichechus inunguis and 26 trichechus manatus were collected. the samples were plated on sabouraud agar with chloramphenicol and incubated at 25 °c for 5 days. the yeasts isolated were phenotypically identified by biochemical and micromorphological tests. overall, 141 strains were isolated, of w ... | 2015 | 26308797 |
| postantifungal effect of micafungin against the species complexes of candida albicans and candida parapsilosis. | micafungin is an effective antifungal agent useful for the therapy of invasive candidiasis. candida albicans is the most common cause of invasive candidiasis; however, infections due to non-c. albicans species, such as candida parapsilosis, are rising. killing and postantifungal effects (pafe) are important factors in both dose interval choice and infection outcome. the aim of this study was to determinate the micafungin pafe against 7 c. albicans strains, 5 candida dubliniensis, 2 candida afric ... | 2015 | 26168269 |
| candida albicans isolates from a malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans candida isolates. | this study was aimed at determining the phospholipase and haemolysin activity of candida isolates in malaysia. a total of 37 candida clinical isolates representing seven species, candida albicans (12), candida tropicalis (8), candida glabrata (4), candida parapsilosis (1), candida krusei (4), candida orthopsilosis (1) and candida rugosa (7) were tested. in vitro phospholipase activity was determined by using egg yolk plate assay whereas in vitro haemolysin activity was tested by using blood plat ... | 2013 | 24522136 |
| species distribution and antifungal susceptibility of bloodstream fungal isolates in paediatric patients in mexico: a nationwide surveillance study. | to establish the species distribution and in vitro susceptibilities of 358 bloodstream fungal isolates from paediatric patients in mexico. | 2013 | 23869052 |
| candida orthopsilosis fungemias in a spanish tertiary care hospital: incidence, epidemiology and antifungal susceptibility. | few studies exist on prevalence of fungemia by candida orthopsilosis, with variable results. | 2014 | 23680638 |
| species distribution and virulence factors of candida spp. isolated from the oral cavity of kidney transplant recipients in brazil. | although yeasts belonging to the genus candida are frequently seen as commensals in the oral cavity, they possess virulence attributes that contribute for pathogenicity. the aims of the present study were to study the prevalence of candida spp. isolated from the oral cavity of renal transplant recipients and to analyze strains virulence factors. we isolated a total of 70 candida strains from 111 transplant recipients, and candida albicans was the most prevalent species (82.86 %). oral candidiasi ... | 2013 | 23539354 |
| rapid and accurate identification of isolates of candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca). | rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. we evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca) as an identification tool for distinguishing between 16 candida spp., i.e. candida albicans, candida bracarensis, candida dubliniensis, candida famata, candida glabrata, candida guilliermondii, candida inconspicua, candida k ... | 2013 | 23142490 |
| voriconazole inhibits biofilm formation in different species of the genus candida. | to determine the ability of voriconazole to inhibit the formation of biofilms. | 2012 | 22733651 |
| sequence and analysis of the genome of the pathogenic yeast candida orthopsilosis. | candida orthopsilosis is closely related to the fungal pathogen candida parapsilosis. however, whereas c. parapsilosis is a major cause of disease in immunosuppressed individuals and in premature neonates, c. orthopsilosis is more rarely associated with infection. we sequenced the c. orthopsilosis genome to facilitate the identification of genes associated with virulence. here, we report the de novo assembly and annotation of the genome of a type 2 isolate of c. orthopsilosis. the sequence was o ... | 2012 | 22563396 |
| epidemiology, species distribution and in vitro antifungal susceptibility of fungaemia in a spanish multicentre prospective survey. | to update the knowledge of the epidemiology of fungaemia episodes in spain, the species implicated and their in vitro antifungal susceptibilities. | 2012 | 22351683 |
| survey and analysis of simple sequence repeats (ssrs) in three genomes of candida species. | simple sequence repeats (ssrs) or microsatellites, which composed of tandem repeated short units of 1-6 bp, have been paying attention continuously. here, the distribution, composition and polymorphism of microsatellites and compound microsatellites were analyzed in three available genomes of candida species (candida dubliniensis, candida glabrata and candida orthopsilosis). the results show that there were 118,047, 66,259 and 61,119 microsatellites in genomes of c. dubliniensis, c. glabrata and ... | 2016 | 26883055 |
| rapid identification of candida spp. frequently involved in invasive mycoses by using flow-through hybridization and gene chip (fhgc) technology. | the incidence of invasive fungal infections in immunocompromised patients has increased in recent decades. rapid and accurate identification of these pathogenic fungi is crucial for initiating a timely, safe, and effective antifungal therapy. here we developed a microarray based on flow-through hybridization gene chip technology. the microarray was tested for its specificity using a panel of reference and blinded clinical isolates. the results proved that this microarray was highly discriminativ ... | 2017 | 27913132 |
| the identification and differentiation of the candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rdna. | currently, it is accepted that there are three species that were formerly grouped under candida parapsilosis: c. para- psilosis sensu stricto, candida orthopsilosis, and candida metapsilosis. in fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences in these nosocomial pathogens. an accurate, fast, and economical identification of fungal species has been the main goal in mycology. in the present study, we searched sequences that were available ... | 2016 | 27074256 |
| candida parapsilosis (sensu lato) isolated from hospitals located in the southeast of brazil: species distribution, antifungal susceptibility and virulence attributes. | candida parapsilosis (sensu lato), which represents a fungal complex composed of three genetically related species - candida parapsilosis sensu stricto, candida orthopsilosis and candida metapsilosis, has emerged as an important yeast causing fungemia worldwide. the goal of the present work was to assess the prevalence, antifungal susceptibility and production of virulence traits in 53 clinical isolates previously identified as c. parapsilosis (sensu lato) obtained from hospitals located in the ... | 2015 | 26319940 |
| molecular identification and antifungal susceptibility profiles of candida parapsilosis complex species isolated from culture collection of clinical samples. | candida parapsilosis is a common yeast species found in cases of onychomycosis and candidemia associated with infected intravascular devices. in this study, we differentiated candida parapsilosis sensu stricto, candida orthopsilosis , and candida metapsilosis from a culture collection containing blood and subungual scraping samples. furthermore, we assessed the in vitro antifungal susceptibility of these species to fluconazole, itraconazole, voriconazole, posaconazole, amphotericin b, and caspof ... | 2015 | 26312937 |
| biofilm formation and genetic variability of bcr1 gene in the candida parapsilosis complex. | candida parapsilosis sensu stricto, candida orthopsilosis, and candida metapsilosis are cryptic species that belong to the c. parapsilosis complex, which has been increasingly associated to fungemia in various geographic regions, principally due to the capability of these yeasts to form biofilms on indwelling medical devices. bcr1 is one of the most studied genes related to candida spp. biofilms. | 2015 | 25843001 |
| clinical characteristics and treatment outcomes of patients with candidaemia due to candida parapsilosis sensu lato species at a medical centre in taiwan, 2000-12. | to investigate the clinical characteristics and treatment outcomes of adult patients with candida parapsilosis sensu lato candidaemia. | 2015 | 25558079 |
| different scenarios for candida parapsilosis fungaemia reveal high numbers of mixed c. parapsilosis and candida orthopsilosis infections. | nosocomial fungal bloodstream infections (bsi) are increasing significantly in hospitalized patients and candida parapsilosis has emerged as an important pathogen responsible for numerous outbreaks. the objective of this study was to evaluate c. parapsilosis sensu lato infection scenarios, regarding species distribution and strain relatedness. one hundred isolates of c. parapsilosis sensu lato derived from blood cultures and catheter tips were analysed by multiplex microsatellite typing and by s ... | 2015 | 25351711 |
| candida parapsilosis sensu stricto and the closely related species candida orthopsilosis and candida metapsilosis in vulvovaginal candidiasis. | this study aimed to determine the clinical characteristics and in vitro susceptibilities of candida parapsilosis sensu stricto, candida orthopsilosis and candida metapsilosis isolates from patients with vulvovaginal candidiasis (vvc). we analysed 63 vaginal c. parapsilosis specimens. after the molecular analyses, the isolates were characterised as c. parapsilosis sensu stricto (77.8%), c. orthopsilosis (7.9%) and c. metapsilosis (14.3%). the signs and symptoms of vvc caused by c. parapsilosis se ... | 2015 | 25322705 |
| antifungal susceptibility and virulence attributes of animal-derived isolates of candida parapsilosis complex. | this study aimed to identify strains of the candida parapsilosis complex isolated from animals, as well as to assess their in vitro antifungal susceptibility profile and in vitro production of virulence attributes. we used 28 isolates of c. parapsilosis sensu lato recovered from clinically healthy animals. the strains were characterized phenotypically, followed by molecular identification of the species through pcr-restriction enzyme analysis. the susceptibility of the strains to amphotericin b, ... | 2014 | 25190736 |
| [prevalence of candida orthopsilosis and candida metapsilosis in clinical samples]. | | 2015 | 25091385 |
| evaluation of the maldi-tof vitek ms™ system for the identification of candida parapsilosis, c. orthopsilosis and c. metapsilosis from bloodstream infections. | twenty-nine candida parapsilosis, seventeen candida orthopsilosis and two candida metapsilosis bloodstream isolates were submitted for identification by vitek-ms™ mass spectrometer. four isolates, two c. orthopsilosis and two c. metapsilosis, were not identified. inclusion of superspectra of both species in this database is required to improve its discrimination power. | 2014 | 25072839 |
| killer behavior within the candida parapsilosis complex. | a group of 29 isolates of candida parapsilosis sensu stricto, 29 of candida orthopsilosis, and 4 of candida metapsilosis were assayed for the presence of killer activity using saccharomyces cerevisiae atcc 26609 as a sensitive strain. all c. metapsilosis isolates showed killer activity at 25 °c while strains of c. parapsilosis sensu stricto or c. orthopsilosis did not exhibit this activity. sensitivity to killer toxins was evaluated using a set of previously reported killer strains of clinical o ... | 2014 | 24898174 |
| fungemia in a spanish hospital: the role of candida parapsilosis over a 15-year period. | candida parapsilosis is one of the main causes of fungemia in tertiary-care hospitals. few studies have analysed the changes in its distribution over a long period. we compared the distribution of c. parapsilosis with that of other fungi over a 15-y period in a tertiary hospital. | 2014 | 24762093 |
| the calcineurin inhibitor cyclosporin a exhibits synergism with antifungals against candida parapsilosis species complex. | candida parapsilosis complex comprises three closely related species, c. parapsilosis sensu stricto, candida metapsilosis and candida orthopsilosis. in the last decade, antifungal resistance to azoles and caspofungin among c. parapsilosis sensu lato strains has been considered a matter of concern worldwide. in the present study, we evaluated the synergistic potential of antifungals and the calcineurin inhibitor cyclosporin a (cys) against planktonic and biofilms of c. parapsilosis complex from c ... | 2014 | 24722799 |
| identification and differentiation of candida parapsilosis complex species by use of exon-primed intron-crossing pcr. | the candida parapsilosis complex is composed of candida parapsilosis sensu stricto, candida orthopsilosis, candida metapsilosis, and the closely related species lodderomyces elongisporus. an exon-primed intron-crossing pcr assay was developed here to distinguish the members of the species complex on the basis of the distinct sizes of amplicons, and candida orthopsilosis and candida metapsilosis were further discriminated by restriction enzyme analysis. | 2014 | 24622093 |
| evaluation of in vivo pathogenicity of candida parapsilosis, candida orthopsilosis, and candida metapsilosis with different enzymatic profiles in a murine model of disseminated candidiasis. | six isolates of the candida parapsilosis complex with different enzymatic profiles were used to induce systemic infection in immunocompetent balb/c mice. fungal tissue burden was determined on days 2, 5, 10, and 15 post challenge. the highest fungal load irrespective of post-infection day was detected in the kidney, followed by the spleen, lung, and liver, with a tendency for the fungal burden to decrease by day 15 in all groups. significant differences among the strains were not detected, sugge ... | 2014 | 24577011 |
| identification and typing of the candida parapsilosis complex: maldi-tof ms vs. aflp. | in this study we compare the capability of amplification fragment-length polymorphism (aflp) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) to identify and subtype isolates of members of the candida parapsilosis complex (c. parapsilosis, c. orthopsilosis, c. metapsilosis) and lodderomyces elongisporus, which cannot be differentiated with biochemical methods. both techniques correctly identified all isolates included in this study and clustered iso ... | 2014 | 24577004 |
| galleria mellonella as a model host to study virulence of candida. | | 2014 | 24384470 |
| candida parapsilosis, candida orthopsilosis, and candida metapsilosis virulence in the non-conventional host galleria mellonella. | the incidence of fungal infections due to c. parapsilosis and closely related cryptic species (-psilosis complex) has increased in the last few years, but differences in virulence among these species have not been widely studied. fifteen clinical isolates of c. parapsilosis, c. orthopsilosis, and c. metapsilosis, including the type strains, were used to evaluate their virulence in galleria mellonella larvae. fluctuations in the hemocyte density and in the phagocytic activity were also tested. di ... | 2014 | 24193303 |
| candidemia and death by candida orthopsilosis and candida metapsilosis in neonates and children. | | 2014 | 24113226 |
| differential sensitivity of the species of candida parapsilosis sensu lato complex against statins. | candida parapsilosis sensu stricto, candida orthopsilosis and candida metapsilosis are human fungal pathogens with clinical importance. the recently reclassified three closely related species have significant variation in virulence, clinical prevalence and susceptibility characteristics to different antifungal compounds. the aim of this study was to investigate the in vitro activity of atorvastatin and fluvastatin against c. metapsilosis, c. orthopsilosis and c. parapsilosis. susceptibility test ... | 2013 | 23943427 |
| analysis of inteins in the candida parapsilosis complex for simple and accurate species identification. | inteins are coding sequences that are transcribed and translated with flanking sequences and then are excised by an autocatalytic process. there are two types of inteins in fungi, mini-inteins and full-length inteins, both of which present a splicing domain containing well-conserved amino acid sequences. full-length inteins also present a homing endonuclease domain that makes the intein a mobile genetic element. these parasitic genetic elements are located in highly conserved genes and may allow ... | 2013 | 23784117 |
| comparison of candida parapsilosis, candida orthopsilosis, and candida metapsilosis adhesive properties and pathogenicity. | retrospective studies indicate that candida metapsilosis and candida orthopsilosis each represents 1-10% of the infections/colonisations attributed to c. parapsilosis by conventional biochemical tests. little is known on the virulence properties of these fungi and on their role in the establishment/progression of the infection. in this study, the adhesive properties of clinical isolates belonging to the 'psilosis' species were assessed in an in vitro model of co-incubation with human buccal epit ... | 2013 | 23403338 |
| aspartyl proteinase, phospholipase, esterase and hemolysin activities of clinical isolates of the candida parapsilosis species complex. | candida parapsilosis is considered as an important emerging fungal pathogen and was recently found to be a complex that include three species, i.e., candida parapsilosis sensu stricto, candida orthopsilosis and candida metapsilosis. the aim of this study was to determine the in vitro aspartyl proteinase, phospholipase, esterase and hemolysin activities of 65 clinical isolates of the c. parapsilosis complex, which had been previously identified by rflp-bani analysis. of the enzymes evaluated, asp ... | 2013 | 22928925 |
| epidemiology and echinocandin susceptibility of candida parapsilosis sensu lato species isolated from bloodstream infections at a spanish university hospital. | the aims of this work were to study the epidemiological profiles, differences in echinocandin susceptibilities and clinical relevance of the candida parapsilosis sensu lato species isolated from proven fungaemia cases at la fe university hospital of valencia (spain) from 1995 to 2007. | 2012 | 22868644 |
| in vitro and in vivo effects of echinocandins against candida parapsilosis sensu stricto, candida orthopsilosis and candida metapsilosis. | the aim of the present study was to compare, in vitro and in vivo, the effects of caspofungin, micafungin and anidulafungin against candida parapsilosis complex isolates. | 2012 | 22635526 |
| accurate identification of candida parapsilosis (sensu lato) by use of mitochondrial dna and real-time pcr. | candida parapsilosis is the candida species isolated the second most frequently from blood cultures in south america and some european countries, such as spain. since 2005, this species has been considered a complex of 3 closely related species: c. parapsilosis, candida metapsilosis, and candida orthopsilosis. here, we describe a real-time taqman-mgb pcr assay, using mitochondrial dna (mtdna) as the target, which readily distinguishes these 3 species. we first used comparative genomics to locate ... | 2012 | 22535986 |
| molecular characterization and antifungal susceptibility of the candida parapsilosis species complex of clinical isolates from monterrey, mexico. | recently, it was proposed that the opportunistic yeast pathogen candida parapsilosis was a complex composed of the following three species: candida parapsilosis sensu stricto, candida orthopsilosis, and candida metapsilosis. a set of 344 clinical isolates of candida parapsilosis from monterrey, mexico was re-identified by rflp. their antifungal susceptibility to fluconazole, caspofungin, anidulafungin and micafungin was determined using the clinical and laboratory standards institute m27-a3 prot ... | 2012 | 22493945 |
| prevalence, distribution and antifungal susceptibility profiles of candida parapsilosis, candida orthopsilosis and candida metapsilosis bloodstream isolates. | the candida parapsilosis group encompasses three species: c. parapsilosis, candida orthopsilosis and candida metapsilosis. these species are phenotypically indistinguishable, and molecular methods are needed for their detection. we analysed 152 unique blood culture isolates of the c. parapsilosis group obtained during 1997-2011. the isolates were screened by pcr amplification of the gene encoding secondary alcohol dehydrogenase, followed by digestion with the restriction enzyme bani. isolates wi ... | 2012 | 22493277 |
| candida "psilosis"--electromigration techniques and maldi-tof mass spectrometry for phenotypical discrimination. | biofilm-positive strains of candida parapsilosis are the second most common yeasts responsible for bloodstream infections. this pathogen is difficult to identify by standard methods from other phenotypically indistinguishable species, biofilm-negative candida orthopsilosis and biofilm-positive candida metapsilosis. from a medical point of view, important information is especially whether the strains form biofilm. the biofilm formation enables yeast to colonize artificial surfaces thereby protect ... | 2012 | 22396948 |
| diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid. | yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ... | 2014 | 24442819 |
| comparison of four molecular approaches to identify candida parapsilosis complex species. | since the description of candida orthopsilosis and c. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from c. parapsilosis sensu stricto. species-specific uniplex polymerase chain reaction (pcr) was performed and compared with sequencing of the d1/d2 region of the lsu 28s rdna gene, microsatellite typing of c. parapsilosis sensu stricto, and pcr-restriction fragment length polymorphism patterns in the its1-5.8s-its2 region of the rdna gene. th ... | 2017 | 28225905 |
| comparison of four molecular approaches to identify candida parapsilosis complex species. | since the description of candida orthopsilosis and c. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from c. parapsilosis sensu stricto. species-specific uniplex polymerase chain reaction (pcr) was performed and compared with sequencing of the d1/d2 region of the lsu 28s rdna gene, microsatellite typing of c. parapsilosis sensu stricto, and pcr-restriction fragment length polymorphism patterns in the its1-5.8s-its2 region of the rdna gene. th ... | 2017 | 28225899 |
| multiple origins of the pathogenic yeast candida orthopsilosis by separate hybridizations between two parental species. | mating between different species produces hybrids that are usually asexual and stuck as diploids, but can also lead to the formation of new species. here, we report the genome sequences of 27 isolates of the pathogenic yeast candida orthopsilosis. we find that most isolates are diploid hybrids, products of mating between two unknown parental species (a and b) that are 5% divergent in sequence. isolates vary greatly in the extent of homogenization between a and b, making their genomes a mosaic of ... | 2016 | 27806045 |
| metabolic profiles of planktonic and biofilm cells of candida orthopsilosis. | this study aims to understand which candida orthopsilosis protein aids fungus adaptation upon its switching from planktonic growth to biofilm. | 2016 | 27662506 |
| simple, low-cost detection of candida parapsilosis complex isolates and molecular fingerprinting of candida orthopsilosis strains in kuwait by its region sequencing and amplified fragment length polymorphism analysis. | candida parapsilosis has now emerged as the second or third most important cause of healthcare-associated candida infections. molecular studies have shown that phenotypically identified c. parapsilosis isolates represent a complex of three species, namely, c. parapsilosis, c. orthopsilosis and c. metapsilosis. lodderomyces elongisporus is another species phenotypically closely related to the c. parapsilosis-complex. the aim of this study was to develop a simple, low cost multiplex (m) pcr assay ... | 2015 | 26580965 |
| a case of candida orthopsilosis associated septic arthritis in a patient with systemic lupus erythematosus (sle). | we report a case of persistent candida orthopsilosis associated septic arthritis. repeated isolation of c. orthopsilosis from tissue and joint fluid was confirmed by identification of the its region of the rrna gene using a candida-specific luminex based assay and gene sequencing of the d1/d2 regions. this was the first case of c. orthopsilosis associated septic arthritis reported in jamaica and in the literature. | 2015 | 27330938 |
| comparative evaluation of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) systems for the identification of clinically significant yeasts. | to prospectively evaluate the performance of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems (maldi-tof ms) for the identification of clinically significant yeast isolates compared to the vitek 2 system. | 2014 | 25080355 |
| occurrence of candida orthopsilosis in brazilian tomato fruits (lycopersicum esculentum mill.). | we aimed to isolate and identify yeasts found in the tomato fruit in order to obtain isolates with biotechnological potential, such as in control of fungal diseases that damage postharvest fruits. we identified candida orthopsilosis strains lt18 and lt24. this is the first report of this yeast on lycopersicum esculentum fruits in brazil. | 2014 | 24948920 |