isolation of potosi virus from aedes albopictus in north carolina. | a total of 4,169 adult mosquitoes were aspirated from 3 tire disposal sites in north carolina for virus assays. aedes albopictus was the dominant species, with a relative abundance of approximately 99%. potosi virus was isolated from one pool of 68 female ae. albopictus. priorities for future potosi virus research and the implications of the north carolina isolate are discussed. | 1995 | 7595449 |
isolations of potosi virus from mosquitoes collected in the united states, 1989-94. | potosi (pot) virus, a recently characterized bunyamwera serogroup virus, was discovered when it was isolated from aedes albopictus collected at a waste-tire site in potosi, washington county, missouri, during 1989. during the following year, pot virus was not isolated from 39,048 mosquitoes, including 17,519 ae. albopictus, collected in washington county. in 1991, mosquito collections from south carolina, ohio, and michigan yielded 8 strains of pot virus: 6 from coquillettidia perturbans and one ... | 1996 | 8723251 |
the role of deer as a possible reservoir host of potosi virus, a newly recognized arbovirus in the united states. | potosi (pot) virus (bunyaviridae) was isolated from aedes albopictus, an introduced asian mosquito species, collected at a used tire yard in potosi, missouri (usa), in august and september, 1989. in september, 1990, small animals were trapped at the tire yard and six cattle were sampled at an adjacent farm; in november 1990 and 1991, blood samples were collected with filter paper strips from 364 hunter-killed, white-tailed deer (odocoileus virginianus) in the region to determine the possible res ... | 1996 | 8827670 |
isolation of la crosse, cache valley, and potosi viruses from aedes mosquitoes (diptera: culicidae) collected at used-tire sites in illinois during 1994-1995. | prospective studies were conducted at used-tire sites in illinois during 1994-1995 in an effort to isolate arboviruses from mosquitoes, particularly aedes albopictus (skuse) and aedes triseriatus (say). three isolates of potosi virus were obtained from ae. albopictus collected at a waste tire site in jasper county during 1994 and 1995. also, a single isolate of cache valley virus was obtained from ae. albopictus collected at the jasper county site during 1995. these are the first records of arbo ... | 1998 | 9701947 |
cache valley and potosi viruses (bunyaviridae) in white-tailed deer (odocoileus virginianus): experimental infections and antibody prevalence in natural populations. | cache valley virus (cvv) and potosi virus (potv) are two closely related mosquito-borne viruses (bunyaviridae: bunyamwera group) that appear to circulate in several regions of the united states, especially the midwest. we determined the prevalence of specific neutralizing antibodies to both viruses in indiana white-tailed deer and conducted infection experiments to assess whether deer could serve as an vertebrate-amplifying host. cross-infection experiments also were carried out to investigate t ... | 1998 | 9840585 |
arbovirus surveillance in south carolina, 1996-98. | arboviruses isolated and identified from mosquitoes in south carolina (usa) are described, including new state records for eastern equine encephalitis virus (eee), st. louis encephalitis virus (sle), flanders virus, tensaw virus (ten), and a variant of jamestown canyon virus (jc). mosquitoes were collected at 52 locations in 30 of 46 south carolina counties beginning in june 1996, and ending in october 1998, and tested for arboviruses. of 1,329 mosquito pools tested by virus isolation (85,806 mo ... | 2001 | 11345423 |
isolations of potosi virus from mosquitoes (diptera: culicidae) collected in connecticut. | potosi virus (potv) (bunyaviridae: orthobunyavirus) was first isolated from aedes albopictus (skuse) collected in potosi, mo, in 1989, and subsequent isolations were reported from illinois, michigan, ohio, and the carolinas. to determine whether the distribution of this virus extends into the northeastern united states, we analyzed arboviruses acquired from mosquitoes collected in connecticut from 1998 to 2004. in 2001, a bunyavirus was isolated from aedes vexans (meigen) that was different from ... | 2005 | 16363172 |
isolation of bunyamwera serogroup viruses (bunyaviridae, orthobunyavirus) in new york state. | during routine arbovirus surveillance from 2000 to 2004 in new york state (nys), 14,788 mosquito pools making up 36 species and nine genera were inoculated onto vero cell cultures to test for abroad spectrum of viruses. forty-six percent of viruses isolated in cell culture from species, excluding culex pipiens l. and culex restuans theobald, were identified as bunyamwera serogroup viruses. here, we report the distribution and level of bunyamwera activity in nys detected during this period. we de ... | 2006 | 17017240 |
detection of infectious virus from field-collected mosquitoes by vero cell culture assay. | mosquitoes transmit a number of distinct viruses including important human pathogens such as west nile virus, dengue virus, and chickungunya virus. many of these viruses have intensified in their endemic ranges and expanded to new territories, necessitating effective surveillance and control programs to respond to these threats. one strategy to monitor virus activity involves collecting large numbers of mosquitoes from endemic sites and testing them for viral infection. in this article, we descr ... | 2011 | 21694689 |
arboviruses in north dakota, 2003-2006. | to investigate arbovirus transmission in north dakota, we collected and screened mosquitoes for viral infection by vero cell culture assay. seven viruses were isolated from 13 mosquito species. spatial and temporal distributions of the important vectors of west nile virus (wnv), cache valley virus, jamestown canyon virus (jcv), and trivittatus virus are reported. snowshoe hare virus, potosi virus, and western equine encephalomyelitis virus were also isolated. the risks of culex tarsalis and aede ... | 2014 | 25487728 |
monitoring sheep and culicoides midges in montana for evidence of bunyamwera serogroup virus infection. | a serological and entomological investigation was performed to monitor for potential bunyamwera (bun) serogroup virus activity in montana. | 2014 | 26392881 |
management factors associated with operation-level prevalence of antibodies to cache valley virus and other bunyamwera serogroup viruses in sheep in the united states. | a cross-sectional study was performed to identify operation-level risk factors associated with prevalence of antibody to bunyamwera (bun) serogroup viruses in sheep in the united states. sera were obtained from 5150 sheep in 270 operations located in 22 states (three in the west, nine central states, and 10 in the east) and tested at a dilution of 1:20 by a plaque reduction neutralization test (prnt) using cache valley virus (cvv). antibodies that neutralized cvv were identified in 1455 (28%) sh ... | 2015 | 26565774 |
sequence and phylogenetic data indicate that an orthobunyavirus recently detected in the yucatan peninsula of mexico is a novel reassortant of potosi and cache valley viruses. | we determined the complete nucleotide sequences of the small (s) and medium (m) rna segments of an orthobunyavirus isolated from mosquitoes in the yucatan peninsula of mexico. a 528-nt region of the large (l) rna segment was also sequenced. the s rna segment has greatest nucleotide identity to the homologous region of cache valley virus (cvv; 98%) followed by potosi virus (potv; 89%) and northway virus (86%). the m rna segment has 96% nucleotide identity to the homologous region of potv, and les ... | 2012 | 22407405 |
host interactions of aedes albopictus, an invasive vector of arboviruses, in virginia, usa. | as an invasive mosquito species in the united states, aedes albopictus is a potential vector of arboviruses including dengue, chikungunya, and zika, and may also be involved in occasional transmission of other arboviruses such as west nile, saint louis encephalitis, eastern equine encephalitis, and la crosse viruses. aedes albopictus feeds on a wide variety of vertebrate hosts, wild and domestic, as well as humans. | 2021 | 33600413 |