| [ngari virus (bunyaviridae: bunyavirus). first isolation from humans in senegal, new mosquito vectors, its epidemiology]. | ngari virus (nri) (bunyaviridae, genus bunyavirus) was isolated first from male aedes simpsoni mosquitoes in southeastern senegal in 1979. then, it was recovered from several mosquito species in senegal, burkina faso, central african republic and madagascar. a potential pathogenicity of nri virus in humans was suspected when the virus was isolated from two patients in dakar in october and november 1993. the large diversity of culicidae vectors and feeding patterns showed a large heterogeneity of ... | 1996 | 8765950 |
| ngari virus is a bunyamwera virus reassortant that can be associated with large outbreaks of hemorrhagic fever in africa. | two isolates of a virus of the genus orthobunyavirus (family bunyaviridae) were obtained from hemorrhagic fever cases during a large disease outbreak in east africa in 1997 and 1998. sequence analysis of regions of the three genomic rna segments of the virus (provisionally referred to as garissa virus) suggested that it was a genetic reassortant virus with s and l segments derived from bunyamwera virus but an m segment from an unidentified virus of the genus orthobunyavirus. while high genetic d ... | 2004 | 15280501 |
| rift valley fever virus. | rift valley fever is considered to be one of the most important viral zoonoses in africa. in 2000, the rift valley fever virus spread to the arabian peninsula and caused two simultaneous outbreaks in yemen and saudi arabia. it is transmitted to ruminants and to humans by mosquitoes. the viral agent is an arbovirus, which belongs to the phlebovirus genus in the bunyaviridae family. this family of viruses comprises more than 300 members grouped into five genera: orthobunyavirus, phlebovirus, hanta ... | 2005 | 16375716 |
| batai and ngari viruses: m segment reassortment and association with severe febrile disease outbreaks in east africa. | ngari virus is an orthobunyavirus recently recognized as a reassortant between bunyamwera virus and an as yet unidentified m segment donor. analysis of m segment sequences of batai and ilesha viruses revealed 95% deduced amino acid identity between batai virus and ngari virus. these findings suggest batai virus as the donor of ngari virus m segment sequence. analysis of batai virus-related african isolates identified ugmp-6830, isolated from mosquitoes in uganda, as an isolate of batai virus. kv ... | 2006 | 16699043 |
| genetic characterization of batai virus indicates a genomic reassortment between orthobunyaviruses in nature. | two viruses were isolated from bovine blood in the southernmost part of japan in 1994 and 2001, respectively. genetic analyses showed that the viruses were batai virus of the genus orthobunyavirus of the family bunyaviridae. the sequencing of three genomic rna segments of the japanese and malaysian batai virus strains revealed that the m rna segment of batai virus had high sequence identity with that of ngari virus. our results indicate that ngari virus is a genetic reassortant with s and l rna ... | 2006 | 16820982 |
| natural m-segment reassortment in potosi and main drain viruses: implications for the evolution of orthobunyaviruses. | recently, we identified batai virus as the m-segment reassortment partner of ngari virus. extension of genetic analyses to other orthobunyaviruses related to the bunyamwera serogroup indicates additional natural genome reassortments. whereas the relative phylogenetic positions of all three genome segment sequences were similar for northway and kairi viruses, the relative positions of potosi and main drain virus m-segment sequences diverged from those of their s- and l-segments. our findings indi ... | 2007 | 17891328 |
| [sequencing and analysis of the full coding sequence of batai virus isolated in china]. | by rt-pcr and tail-pcr, the full coding region of batai virus isolated in china (yn92-4 strain)was sequenced for the first time. according to the results, the genome of the virus contained three segments s, m and l of 947, 4,371 and 6,860 nucleotides, respectively. the s segment coded a nucleoprotein of 234 amino acids and a nonstructural protein of 102 amino acids, the m and l segments coded a precursor protein of 1 ,435 amino acids and rna polymerase of 2,239 amino acids, respectively. compare ... | 2009 | 19678560 |
| mosquito-borne arbovirus surveillance at selected sites in diverse ecological zones of kenya; 2007 - 2012. | increased frequency of arbovirus outbreaks in east africa necessitated the determination of distribution of risk by entomologic arbovirus surveillance. a systematic vector surveillance programme spanning 5 years and covering 11 sites representing seven of the eight provinces in kenya and located in diverse ecological zones was carried out. | 2013 | 23663381 |
| genome sequence analysis of in vitro and in vivo phenotypes of bunyamwera and ngari virus isolates from northern kenya. | biological phenotypes of tri-segmented arboviruses display characteristics that map to mutation/s in the s, m or l segments of the genome. plaque variants have been characterized for other viruses displaying varied phenotypes including attenuation in growth and/or pathogenesis. in order to characterize variants of bunyamwera and ngari viruses, we isolated individual plaque size variants; small plaque (sp) and large plaque (lp) and determined in vitro growth properties and in vivo pathogenesis in ... | 2014 | 25153316 |
| ngari virus in goats during rift valley fever outbreak, mauritania, 2010. | | 0 | 25419696 |
| complete genome sequencing of mosquito and human isolates of ngari virus. | ngari virus (nriv) is a recently described, naturally occurring reassortant between two other orthobunyaviruses, bunyamwera virus (bunv) and batai virus (batv). intriguingly, this reassortment was associated with the acquisition of heightened virulence, although the molecular basis for this is not understood. here we report the first complete genome sequences of ngari virus. we include five isolates from various geographical locations, as well as samples isolated from both mosquitos and human ca ... | 0 | 23166252 |
| serological evidence of batai virus infections, bovines, northern italy, 2011. | batai virus (batv) was identified in mosquitoes in the caltignaga region of novarra, northern italy in 2009. here, we report the identification of antibodies to batv in serum samples that were taken from healthy bovines in that region in 2011. batv has been associated with a mild febrile human illness and identified as the likely parental segment donor in a reassortment event that resulted in the generation of the virulent progeny, ngari virus. the possible veterinary disease associations of bat ... | 2014 | 25198523 |
| molecular characterization of the african orthobunyavirus ilesha virus. | ilesha virus is an arthropod-borne virus belonging to the genus orthobunyavirus of the bunyaviridae family. ilesha virus has been isolated from humans in several african countries, mostly in relation with febrile illness and erythema, though there are reported cases of fatal meningoencephalitis and hemorrhagic fever. in the present study, we report the complete genomic sequence of all three ilesha virus segments (s, m, l) and characterize the open reading frames. the nucleoprotein encoded by seg ... | 2013 | 23988729 |
| vector competence of selected mosquito species in kenya for ngari and bunyamwera viruses. | bunyamwera and ngari viruses have been isolated from a range of mosquito species in kenya but their actual role in the maintenance and transmission of these viruses in nature remains unclear. identification of the mosquito species efficient in transmitting these viruses is critical for estimating the risk of human exposure and understanding the transmission and maintenance mechanism. we determined the vector competence of, aedes aegypti (l.), culex quinquefasciatus say, and anopheles gambiae gil ... | 2014 | 26309314 |
| phylogenetic analysis of bunyamwera and ngari viruses (family bunyaviridae, genus orthobunyavirus) isolated in kenya. | orthobunyaviruses, tri-segmented, negative-sense rna viruses, have long been associated with mild to severe human disease in africa, but not haemorrhagic fever. however, during a rift valley fever outbreak in east africa in 1997-1998, ngari virus was isolated from two patients and antibody detected in several others with haemorrhagic fever. the isolates were used to identify ngari virus as a natural orthobunyavirus reassortant. despite their potential to reassort and cause severe human disease, ... | 2016 | 26118981 |
| clarifying bunyamwera virus riddles of the past. | pyrosequencing data and phylogenetic analysis for the full genome of ilesha virus, ngari virus and calovo virus are described clarifying their much discussed relationship within the species bunyamwera virus of the genus orthobunyavirus of the bunyaviridae. | 2013 | 23686694 |
| ticks and tick-borne viruses from livestock hosts in arid and semiarid regions of the eastern and northeastern parts of kenya. | biodiversity and relative abundance of ticks and associated arboviruses in garissa (northeastern) and isiolo (eastern) provinces of kenya were evaluated. ticks were collected from livestock, identified to species, pooled, and processed for virus isolation. in garissa, rhipicephalus pulchellus gerstacker (57.8%) and hyalomma truncatum koch (27.8%) were the most abundant species sampled, whereas r. pulchellus (80.4%) and amblyomma gemma donitz (9.6%) were the most abundant in isiolo. forty-four vi ... | 2014 | 24605478 |
| association of batai virus infection and encephalitis in harbor seals, germany, 2016. | we isolated batai virus from the brain of a euthanized, 26-year-old, captive harbor seal with meningoencephalomyelitis in germany. we provide evidence that this orthobunyavirus can naturally infect the central nervous system of a mammal. the full-genome sequence showed differences from a previously reported virus isolate from a mosquito in germany. | 2018 | 30124416 |