| candida bracarensis sp. nov., a novel anamorphic yeast species phenotypically similar to candida glabrata. | two yeast strains, 153mt and ncyc 3133, isolated from clinical sources in separate hospitals were found to be almost identical in the sequences of the d1/d2 domain of large-subunit rdna, the pcr fingerprinting profiles and physiological characteristics. the isolates are phenotypically similar, although not identical, to candida glabrata and kluyveromyces delphensis (recently renamed nakaseomyces delphensis). sequence analysis of the 26s rdna d1/d2 gene variable region revealed that the two clini ... | 2006 | 16403904 |
| candida bracarensis detected among isolates of candida glabrata by peptide nucleic acid fluorescence in situ hybridization: susceptibility data and documentation of presumed infection. | molecular taxonomic studies have revealed new candida species among phenotypically delineated species, the best example being candida dubliniensis. this study was designed to determine the occurrence of two new molecularly defined species, candida bracarensis and candida nivariensis, which are closely related to and identified as candida glabrata by phenotypic assays. a total of 137 recent clinical isolates of c. glabrata identified by phenotypic characteristics was tested with c. bracarensis an ... | 2008 | 18077641 |
| identification of candida nivariensis and candida bracarensis in a large global collection of candida glabrata isolates: comparison to the literature. | we analyzed 1,598 candida glabrata isolates for the presence of the cryptic species candida nivariensis and candida bracarensis. both species were very rare in this collection (0.2% prevalence), despite the number of isolates analyzed and the global distribution of the isolates. we saw no associated antifungal resistance in c. nivariensis. | 2009 | 19193845 |
| a multiplex pcr protocol for rapid identification of candida glabrata and its phylogenetically related species candida nivariensis and candida bracarensis. | we have developed a multiplex pcr protocol for the detection of candida glabrata and its closely related species candida nivariensis and candida bracarensis. the method uses four pcr primers, targeting the its1 region and the 5.8s ribosomal rna gene. the combination of these primers yielded unique results to all candida species tested. the pcr assay we developed was found to be a rapid, specific and easy to perform method and it will be useful for characterizing large numbers of isolates for epi ... | 2009 | 19635503 |
| candida bracarensis bloodstream infection in an immunocompromised patient. | candida bracarensis is a recently described candida species which is phenotypically similar to candida glabrata. a case of c. bracarensis bloodstream infection in a bone marrow transplant patient is described and confirms this organism as an opportunistic human pathogen. the organism can be distinguished from c. glabrata by its white color on chromagar and by dna sequence analysis using d1/d2 and internal transcribed spacer (its) primers. | 2010 | 20881164 |
| prevalence of candida bracarensis and candida nivariensis in a spanish collection of yeasts: comparison of results from a reference centre and from a population-based surveillance study of candidemia. | two new species related to candida glabrata, i.e., candida nivariensis and candida bracarensis, have been proposed. the occurrence of these species among isolates collected in a spanish mycology reference laboratory in 2008-2009 was reviewed. in addition, strains recovered as part of an active population-based surveillance of candidemia conducted in barcelona between 2002 and 2003 were also analyzed. among 143 clinical isolates received in 2008-2009, three (2%) were identified as c. bracarensis ... | 2011 | 21198347 |
| candida uthaithanina sp. nov., an anamorphic yeast species in nakaseomyces clade isolated in thailand. | three yeast stains were isolated from two unknown fruits (strains dd2-22-1(t) and sk44) and moss (strain st-449) in thailand. analysis of the d1/d2 domain of the large subunit (lsu) rrna gene sequences of the three strains revealed that they belonged to the same species. in terms of pairwise sequence similarity, candida cf. glabrata uwo(ps) 98-110.4 and candida nivariensis were the closest undescribed and recognized taxa, but the levels of nucleotide substitutions were 1.7-1.9% and 2.0-2.2%, res ... | 2011 | 21311971 |
| prevalence and antifungal susceptibility patterns of new cryptic species inside the species complexes candida parapsilosis and candida glabrata among blood isolates from a spanish tertiary hospital. | objectives there is scarce information on the clinical relevance and antifungal susceptibility of candida bracarensis, candida nivariensis, candida orthopsilosis and candida metapsilosis. the objective of this study was to assess the prevalence and in vitro antifungal susceptibility of these cryptic species among 173 blood isolates previously identified as candida glabrata or candida parapsilosis at the hospital of cruces (barakaldo, spain). the survey was extended to 518 clinical isolates from ... | 2011 | 21795259 |
| rapid discrimination between candida glabrata, candida nivariensis and candida bracarensis using a simple-plex pcr. | we report here a pcr-based assay using a single primer pair targeting the rpl31 gene that allows the discrimination between candida glabrata, c. bracarensis and c. nivariensis according to the size of the generated amplicon. | 2011 | 21752976 |
| rapid and accurate identification of isolates of candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca). | rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. we evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca) as an identification tool for distinguishing between 16 candida spp., i.e. candida albicans, candida bracarensis, candida dubliniensis, candida famata, candida glabrata, candida guilliermondii, candida inconspicua, candida k ... | 2013 | 23142490 |
| epidemiology, species distribution and in vitro antifungal susceptibility of fungaemia in a spanish multicentre prospective survey. | to update the knowledge of the epidemiology of fungaemia episodes in spain, the species implicated and their in vitro antifungal susceptibilities. | 2012 | 22351683 |
| evaluation of the commercial rapid trehalose test (glabrata rtt) for the point of isolation identification of candida glabrata isolates in primary cultures. | candidaemias account for 10-20% of nosocomial bloodstream infections depending on the study. whilst candida albicans remains the most frequently isolated species, candida glabrata may be responsible for as many as 10-25% of all candidaemias. moreover, c. glabrata is generally less susceptible to the azole antifungals than the majority of other pathogenic yeast species. thus, a rapid test for the specific identification of isolates of c. glabrata would be useful for patient management if it could ... | 2012 | 22234529 |
| identification and antifungal susceptibility profiles of candida nivariensis and candida bracarensis in a multi-center chinese collection of yeasts. | candida nivariensis and c. bracarensis are two emerging cryptic species within the c. glabrata complex. thirteen of these isolates from 10 hospitals in china were studied for their species identification and antifungal susceptibilities. phenotypic and molecular [rdna its sequencing, d1/d2 sequencing and its sequencer-based capillary gel electrophoresis (scge)] and matrix-assisted laser desorption ionization-time of flight (maldi-tof) ms identification methods were compared for their performance ... | 2017 | 28154553 |
| multiplex pcr designed to differentiate species within the candida glabrata complex. | no phenotypic methods are available to unequivocally differentiate species within the candida glabrata complex. | 2017 | 27810263 |
| candida glabrata species complex prevalence and antifungal susceptibility testing in a culture collection: first description of candida nivariensis in argentina. | the presence of the cryptic species belonging to the candida glabrata complex has not been studied in argentina. we analyzed a collection of 117 clinical isolates of c. glabrata complex belonging to a national culture collection of instituto nacional de microbiología "dr. carlos g. malbrán" from argentina (40 isolates from blood samples, 18 from other normally sterile sites, 20 from vagina, 14 from urine, 7 from oral cavity, 3 from catheter, 1 from a stool sample and 14 isolates whose clinical o ... | 2016 | 27681573 |
| candida bracarensis: evaluation of virulence factors and its tolerance to amphotericin b and fluconazole. | candida bracarensis is an uncommon candida species found during an epidemiological study of candidiasis performed in braga, portugal. initially, it was identified as c. glabrata, but recently detailed analyses pointed out their differences. so, little information is still available about c. bracarensis virulence factors and antifungal susceptibilities. therefore, the main goal of this work is to evaluate the ability of c. bracarensis to form biofilms, to produce hydrolytic enzymes (proteases, ph ... | 2015 | 26179982 |
| in vitro fungicidal activities of anidulafungin, caspofungin, and micafungin against candida glabrata, candida bracarensis, and candida nivariensis evaluated by time-kill studies. | anidulafungin, caspofungin, and micafungin killing activities against candida glabrata, candida bracarensis, and candida nivariensis were evaluated by the time-kill methodology. the concentrations assayed were 0.06, 0.125, and 0.5 μg/ml, which are achieved in serum. anidulafungin and micafungin required between 13 and 26 h to reach the fungicidal endpoint (99.9% killing) against c. glabrata and c. bracarensis. all echinocandins were less active against c. nivariensis. | 2015 | 25801575 |
| candida nivariensis in comparison to different phenotypes of candida glabrata. | the purpose of the study was to establish the prevalence of new candida glabrata complex species: candida nivariensis and candida bracarensis isolated from clinical material, evaluate their phenotypes and the prevalence of gene family encoding extracellular glycosylphosphatidylinositol-linked aspartyl proteases, crucial for c. glabrata virulence. study material included 224 c. glabrata clinical strains. candida glabrata phenotypes were identified using chromagar candida medium. strains were anal ... | 2014 | 25267284 |
| prevalence of candida nivariensis and candida bracarensis in vulvovaginal candidiasis. | candida nivariensis and candida bracarensis were isolated from patients with vulvovaginal candidiasis (vvc). candida nivariensis and candida bracarensis were found in presumptive candida glabrata isolates, which were identified using the api candida system. we retrospectively re-examined vaginal presumptive candida glabrata isolates for candida nivariensis and candida bracarensis from january 1, 2003, through december 31, 2012, via detection of the its1 region and the 5.8s ribosomal rna gene. am ... | 2014 | 25118875 |
| [first case report of catheter-related fungemia by candida nivariensis in the iberian peninsula]. | in recent years the incidence of candidemia caused by non-albicans candida species has been increasing. two cryptic species have been described within the candida glabrata complex, candida nivariensis and candida bracarensis, which may be troublesome in laboratory identification and have lower susceptibility to fluconazole. | 2013 | 22982698 |
| validation of the pcr-dhplc method for rapid identification of candida glabrata phylogenetically related species in different biological matrices. | since two new species phylogenetically related to candida glabrata with slightly different phenotypes and antifungal susceptibility profiles have been described, it seems to be necessary from clinical point of view, to develop a rapid and accurate identification system in order to distinguish between these three fungal species. we studied the performance of denaturing high performance liquid chromatography (dhplc) as a faster (less than 7 min) and alternative novel technique for simultaneous ana ... | 2012 | 22456535 |