| assembly of viral metagenomes from yellowstone hot springs. | thermophilic viruses were reported decades ago; however, knowledge of their diversity, biology, and ecological impact is limited. previous research on thermophilic viruses focused on cultivated strains. this study examined metagenomic profiles of viruses directly isolated from two mildly alkaline hot springs, bear paw (74 degrees c) and octopus (93 degrees c). using a new method for constructing libraries from picograms of dna, nearly 30 mb of viral dna sequence was determined. in contrast to pr ... | 2008 | 18441115 |
| morphology and genome organization of the virus psv of the hyperthermophilic archaeal genera pyrobaculum and thermoproteus: a novel virus family, the globuloviridae. | a novel virus, termed pyrobaculum spherical virus (psv), is described that infects anaerobic hyperthermophilic archaea of the genera pyrobaculum and thermoproteus. spherical enveloped virions, about 100 nm in diameter, contain a major multimeric 33-kda protein and host-derived lipids. a viral envelope encases a superhelical nucleoprotein core containing linear double-stranded dna. the psv infection cycle does not cause lysis of host cells. the viral genome was sequenced and contains 28337 bp. th ... | 2004 | 15193919 |