| enzymatic synthesis of methylbutenol from dimethylallyl diphosphate in needles of pinus sabiniana. | methylbutenol (2-methyl-3-buten-2-ol) is an abundant volatile organic compound released from western u.s. pines. to understand the mechanism of methylbutenol formation, we developed a sensitive gas chromatographic assay for its detection and determined that needles of gray pine (pinus sabiniana) contain an enzyme that catalyzes the synthesis of methylbutenol from dimethylallyl diphosphate (dmapp). the methylbutenol synthase activity was partially purified; its ph optimum was 7-8, and, like other ... | 2000 | 11097185 |
| rapid nitrogen transfer from ectomycorrhizal pines to adjacent ectomycorrhizal and arbuscular mycorrhizal plants in a california oak woodland. | nitrogen transfer among plants in a california oak woodland was examined in a pulse-labeling study using 15n. the study was designed to examine n movement among plants that were mycorrhizal with ectomycorrhizas (em), arbuscular mycorrhizas (am), or both. isotopically enriched n (k15no3-) was applied to gray pine (pinus sabiniana) foliage (donor) and traced to neighboring gray pine, blue oak (quercus douglasii), buckbrush (ceanothus cuneatus) and herbaceous annuals (cynosurus echinatus, torilis a ... | 2006 | 16539611 |
| are true multihost fungi the exception or the rule? dominant ectomycorrhizal fungi on pinus sabiniana differ from those on co-occurring quercus species. | | 2009 | 19302178 |
| analysis of sesquiterpene emissions by plants using solid phase microextraction. | solid phase microextraction (spme) was characterized for the sampling and analysis of sesquiterpenes (sqts) emitted by plants. constant mixing ratio sqt standards were produced using a capillary diffusion system. polydimethylsiloxane spme fibers were characterized with respect to relative absorption of sqts, and the effects of sample linear velocity and sample relative humidity on sqt absorption. spme was then utilized to measure sqt emissions from gray pine (pinus sabiniana) and ponderosa pine ... | 2009 | 19819461 |
| a molecular survey of ectomycorrhizal hyphae in a california quercus-pinus woodland. | ectomycorrhizal (ecm) hyphal communities have not been well characterized. furthermore, there have been few studies where the ecm hyphal community is compared to fungi detected as sporocarps or ecm-colonized root tips. we investigated fungi present as hyphae in a well-studied california quercus-pinus woodland. hyphal species present were compared to those found as sporocarps and ecm root tips at the same site. hyphae were extracted from root-restrictive nylon mesh in-growth bags buried in the so ... | 2010 | 19826841 |
| litter contributions to dissolved organic matter and disinfection byproduct precursors in california oak woodland watersheds. | export of dissolved organic matter (dom) from california oak woodland ecosystems is of a great concern because dom is a precursor for carcinogenic disinfection byproducts (dbps) formed during drinking water treatment. fresh litter and decomposed duff materials for the four dominant vegetation components of california oak woodlands: blue oak (quercus douglassi h. & a.), live oak (quercus wislizenii a. dc.), foothill pine (pinus sabiniana dougl.), and annual grasses, were exposed in natural condit ... | 2009 | 19875789 |
| biochemical characterization and homology modeling of methylbutenol synthase and implications for understanding hemiterpene synthase evolution in plants. | 2-methyl-3-buten-2-ol (mbo) is a five-carbon alcohol produced and emitted in large quantities by many species of pine native to western north america. mbo is structurally and biosynthetically related to isoprene and can have an important impact on regional atmospheric chemistry. the gene for mbo synthase was identified from pinus sabiniana, and the protein encoded was functionally characterized. mbo synthase is a bifunctional enzyme that produces both mbo and isoprene in a ratio of ∼90:1. divale ... | 2011 | 21504898 |
| a nitrogen fertilization field study of carbon-13 and nitrogen-15 transfers in ectomycorrhizas of pinus sabiniana. | ectomycorrhizal (em) fungi form relationships with higher plants; plants transfer c to fungi, and fungi transfer nutrients to their host. while evidence indicates that this interaction is largely mutualistic, less is known about how nutrient supply and em associates may alter c and nutrient exchanges, especially in intact plant-soil-microbe systems in the field. in a dual-labeling experiment with n fertilization, we used c and n stable isotopes to examine in situ transfers in em pine trees in a ... | 2013 | 23912260 |
| engineering an isoprenoid pathway in escherichia coli for production of 2-methyl-3-buten-2-ol: a potential biofuel. | 2-methyl-3-buten-2-ol (mbo) is a natural volatile 5-carbon alcohol produced by several pine species that have the potential to be used as biofuel. mbo has a high energy content making it superior to ethanol in terms of energy output, and due to its volatility and lower solubility in water, mbo is easier to recover than ethanol. pine's mbo synthase enzyme utilizes the intermediate dimethylallyl pyrophosphate (dmapp) produced by the methyl-erythritol-4-phosphate isoprenoid pathway for the producti ... | 2014 | 24271564 |