| anti-inflammatory and anthelmintic activities of solanum khasianum clarke. | in order to scientifically appraise some of the folkloric uses of solanum khasianum clarke (solanaceae), the present study was undertaken to examine the anti-inflammatory and anthelmintic properties of the berries of ethanol extract. anti-inflammatory activity was tested in carrageenan induced rat hind paw edema method at three dose level of 200, 300, and 400 mg kg(-1) respectively, diclofenac sodium (100 mg kg(-1)) was used as the reference standard. the anti-inflammatory activity of the extrac ... | 2008 | 18266159 |
| sequential injection analysis with lab-at-valve (si-lav) for the determination of solasodine in solanum species. | the development of sequential injection analysis with lab-at-valve (lav) semi-automated system on-line liquid-liquid extraction is demonstrated for spectrophotometric determination of solasodine in various solanum species fruits. the main proposed is semi-automated extractive determination of solasodine using methyl orange as colorimetric reagent. after optimization of the system, sample, reagent and organic solvent were sequentially aspirated into an extraction coil connected to the center of a ... | 2010 | 20188963 |
| rapid separation of solasodine glycosides by an immunoaffinity column using anti-solamargine monoclonal antibody. | immunoaffinity column using anti-solamargine monoclonal antibody for separation of solasodine glycosides was established. this method was specific for solasodine glycosides which was detected by thin layer chromatography and the western blotting. total solasodine glycosides have been separated directly from the crude extract of solanum khasianum fruit by the newly established immunoaffinity column. | 1999 | 19003135 |
| a novel glucosyltransferase involved in steroid saponin biosynthesis in solanum aculeatissimum. | steroidal saponins are widely distributed in many plant species. their diverse structures have resulted in a wide range of applications, including drug and medicine production. it has been suggested that the nature of the non-saccharide and oligosaccharide portions of the saponin molecule both contribute to the properties of individual saponins. despite numerous studies on the occurrence, chemical structure, and varying pharmaceutical activities of steroidal saponins, their biosynthesis pathway ... | 2005 | 15821879 |
| cold-responsive mirnas and their target genes in the wild eggplant species solanum aculeatissimum. | low temperature is an important abiotic stress in plant growth and development, especially for thermophilic plants. eggplants are thermophilic vegetables, although the molecular mechanism of their response to cold stress remains to be elucidated. micrornas (mirnas) are a class of endogenous small non-coding rnas that play an essential role during plant development and stress responses. although the role of many plant mirnas in facilitating chilling tolerance has been verified, little is known ab ... | 2017 | 29287583 |
| characterization and engineering of glycosyltransferases responsible for steroid saponin biosynthesis in solanaceous plants. | solanaceous plants contain steroid saponins that have diverse biological and pharmacological activities. the structures of their sugar chains play an important role in their activities. a functional glucosyltransferase sagt4a from solanum aculeatissimum glucosylates both steroidal sapogenins and steroidal alkaloids. a potato (s. tuberosum) glycosyltransferase stsgt, which has a high degree of sequence homology with sagt4a, exhibits the same substrate specificity toward steroidal compounds as sag ... | 2007 | 17204296 |
| growth and steroidal saponin production in hairy root cultures of solanum aculeatissimum. | hairy root cultures of solanum aculeatissimum were established by trans-formation using agrobacterium rhizogenes strain 15834. root growth and production of steroidal saponin were investigated under various culture conditions. transformed roots grew better in gamborg's b5 medium containing 3 % sucrose under continuous light than in the dark. also, the roots turned light green when cultured under continuous light. green hairy roots produced aculeatiside a (6.71mg ·) l(-1) and aculeatiside b (6.39 ... | 1995 | 24185447 |
| elicitation based enhancement of secondary metabolites in rauwolfia serpentina and solanum khasianum hairy root cultures. | rauwolfia serpentina and solanum khasianum are well-known medicinally important plants contained important alkaloids in their different parts. elicitation of these alkaloids is important because of associated pharmaceutical properties. targeted metabolites were ajmaline and ajmalicine in r. serpentina; solasodine and α-solanine in s. khasianum. | 2016 | 27563218 |
| technology of compact mab and its application for medicinal plant breeding named as missile type molecular breeding. | single chain fragment-variable (scfv) enhanced solasodine glycoside accumulation in solanum khasianum hairy root cultures transformed by the scfv solamargine (as)-scfv gene. the scfv protein was expressed at a high level in inclusion bodies of e. coli. after being renatured, the scfv protein was purified in a one-step manner by metal chelate affinity chromatography. the yield of refolded and purified scfv was 12.5 mg per 100 ml of cell culture. the characteristics of the as-scfv expressed in e. ... | 2011 | 21143130 |
| anti-solasodine glycoside single-chain fv antibody stimulates biosynthesis of solasodine glycoside in plants. | we constructed a recombinant antibody fragment--single chain fragment-variable (scfv) antibody--derived from hybridoma cell lines to control the concentration of solasodine glycosides in hairy root cultures of solanum khasianum transformed by the anti-solamargine (as)-scfv gene. the properties of the as-scfv protein expressed in escherichia coli were almost identical to those of the parent monoclonal antibody (mab). up to 220 ng recombinant as-scfv was expressed per milligram of soluble protein ... | 2003 | 14504907 |
| effect of fruit stage and maturity on the gluco-alkaloid content in solanum khasianum. | | 1972 | 5018496 |
| effect of seed number and fruit weight on content and localization of glycoalkaloids in solanum khasianum. | | 1981 | 17401982 |
| a simple method for purification of glycoalkaloids in the quantitative estimation of solanum khasianum. | the property of hydroxylapatite (ca (5) (po (4)) (3) oh) to adsorb plant components selectively is used to isolate and purify steroid glycoalkaloids from solanum khasianum. this method is suitable for botanists seeking higher alkaloid yielding plants and can be performed in experimental plantations where limited laboratory facilities are available. | 1981 | 17402013 |
| plant regeneration from cultured hypocotyl explants of diploid and tetraploid solanum khasianum clarke. | plant regeneration through shoot bud formation was induced in cultured hypocotyl explants of diploid and tetraploid varieties of solanum khasianum. optimum regeneration of multiple shoot buds accompanied by rooting occurred on murashige and skoog's medium supplemented with iaa (1 mg/1) and kinetin or benzyladenine (1 mg/1). plantlets obtained in vitro were successfully established in soil. | 1982 | 24257708 |
| association between berry characters and solasodine content of solanum khasianum. | correlation between number, grren weight, dry weight and solasodine percent of berry of the treated and control plant progenies in second generation raised from x-rayed (5 kr, 7.5 kr, 10 kr, 12.5 kr and 15 kr), ems-treated (0.5%, 0.75% and 1% solution in po (4)-buffer) and untreated dry seeds of solanum khasianum clarke revealed that only the numbers of large and small berries had significant correlation with solasodine percent. correlation between induced variations (cv%) in these characters in ... | 1983 | 17404959 |
| the effect of water stress on growth and solasodine content in solanum khasianum. | the effect of water stress on growth and development of solanum khasianum (clarke) and on the solasodine content of its fruits, was studied under mild (field experiment) and severe (pot experiment) conditions. under field conditions, vegetative development was inhibited but fruit production or yield were not affected and fruit maturity was enhanced considerably. solasodine content was not affected and yield per plant remained the same with and without irrigation. under conditions of severe water ... | 1984 | 17340252 |
| growth and production of steroid saponin in solanum aculeatissimum during one vegetation period. | solanum aculeatissimum (solanaceae) plants, which were cultivated in the open field, were harvested once a month from june 1985 to january 1986. the growth matter and content of steroid saponins, aculeatiside a and b which are precursors of steroid hormones in root, were then measured in order to examine their seasonal variations during one vegetation period. the aerial parts of the plants died of chill and frost in january. in november the dry weight of root reached the maximum, and the content ... | 1988 | 17265223 |
| interspecific somatic hybrids between solanum khasianum and s. aculeatissimum produced by electrofusion. | mesophyll derived protoplasts of solanum khasianum clarke and s. aculeatissimum jacq. were fused by electrofusion and resulted in interspecific hybrids. in total, 249 calluses were obtained from which more than 65% produced shoots. from the regenerated plantlets 45% could be identified as hybrids by chromosome counts (4x = 48) and esterase isozyme pattern. most hybrids showing intermediate phenotypic characteristics, had a normal flower morphology with fertile pollen and produced fertile berries ... | 1994 | 24193650 |
| effect of transplanting time on incidence of alternataria leaf blight in solanum khasianum clark. | solanum khasi clark is a shrub which yields solasodine, a steroidal alkaloid. transplating of seedlings of s. khasianum during november was found to be favourable for development of alternaria leaf blight followed by transplanting in december, january and february. this paper presents the findings of the investigation. | 1997 | 22556822 |
| solakhasoside, a novel steroidal saponin from solanum khasianum. | solakhasoside (1), a novel steroidal saponin, was isolated from the fruit of solanum khasianum. its structure was determined as (23s, 25s)-spirot-5-en-3beta,17alpha, 23-triol-3-o-[alpha-l-rhamnopyranosyl-(1-->2)[beta-d-xylopyranosyl-(1 -->3)]-beta-d-galactopyranoside] (1) by spectroscopic analysis. | 1999 | 9917316 |
| survey of solasodine-type glycoalkaloids by western blotting and elisa using anti-solamargine monoclonal antibody. | solasodine glycosides were analyzed for the selection of higher-producing natural resources in the fruits of solanum spp. by western blotting and elisa using anti-solamargine monoclonal antibody (mab). western blotting of solanum khasianum showed a correlation with the analysis by elisa. the results indicated that the fruits of s. khasianum contained the highest level of solasodine glycosides, compared to other species having 53.43 +/- 3.90 microg/mg dry wt. the high level of solasodine glycosid ... | 2000 | 10706414 |
| isolation of solasodine and other steroidal alkaloids and sapogenins by direct hydrolysis-extraction of solanum plants or glycosides therefrom. | concomitant extraction and hydrolysis of solanum steroidal glycoalkaloids in a two-phase system containing an aqueous mineral acid and an organic water immiscible solvent, and having a boiling point under 100(o)c, is described. it is essentially a "one-pot" process, combining direct acid hydrolysis of the glycosides in the plant material and in situ extraction of the released aglycones after alkali treatment, in a single step, the various ingredients being added simultaneously or sequentially, a ... | 2001 | 11557084 |